Abstract：
:Extrusion of metabolites (glycerol, lactic, malic, and succinic acid) during the medium acidification caused by resting baker's yeast supplied with 200 mM glucose was studied under aerobic and anaerobic conditions and in the absence and presence of 14 mM KCl. The maximum levels of glycerol and of the sum of acids (about 13 and 8 mM, respectively) were attained anaerobically; aerobiosis reduced the levels by 40-50% and the presence of K+ ions by another 10-20%. The time courses of glucose consumption and medium acidification were similar aerobically and anaerobically. The glucose consumption curves exhibited a short plateau about 2 min after glucose addition, caused probably by a rapid osmotic equilibration of glucose across the cell mambrane. Metabolite extrusion indicates that at high glucose concentrations the alcohol dehydrogenase reaction is supplemented by other reactions aiding in the maintenance of a balanced NAD+/NADH ratio in the cells.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Sigler K,Knotková A,Páca J,Wurst Mdoi
10.1007/BF02876611subject
Has Abstractpub_date
1980-01-01 00:00:00pages
311-7issue
4eissn
0015-5632issn
1874-9356journal_volume
25pub_type
杂志文章abstract：:The biological activity and the presence of genes sfp and ituD (surfactin and iturin A) among Bacillus strains isolated from the Amazon basin were determined. Bacillus spp. were tested for hemolytic activity and inhibition of fungal growth by agar plate assays in parallel with PCR for identification of sfp and ituD ge...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-011-0056-7
更新日期：2011-07-01 00:00:00
abstract：:Candidiases, infections caused by germination forms of the Candida fungus, represent a heterogeneous group of diseases from systemic infection, through mucocutaneous form, to vulvovaginal form. Although caused by one organism, each form is controlled by distinct host immune mechanisms. Phagocytosis by polymorphonuclea...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/BF02931313
更新日期：2007-01-01 00:00:00
abstract：:Poly-gamma-L-glutamic acid was synthesized in optimized fermentor cultivation of Bacillus licheniformis. The polypeptide obtained was esterified using benzyl- and butylbromides. Benzyl esters with 30, 60 and 100% degree of esterification were prepared. Solubility of the products in dimethyl sulfoxide, chloroform, meth...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02818530
更新日期：2001-01-01 00:00:00
abstract：:This study presents the effects of the probiotic and enterocin M-producing strain Enterococcus faecium AL41 on microbiota, phagocytic activity (PA), oxidative stress, performance and biochemical parameters when applied individually or in combination with Eleutherococcus senticosus in rabbits. The novelty of the study ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-015-0423-x
更新日期：2016-03-01 00:00:00
abstract：:The ability of Phanerochaete chrysosporium, Trametes versicolor, Coriolopsis polyzona, and Pleurotus ostreatus growing in a mitogen-limited mineral medium (NMM) to degrade PCBs in a commercial, Delor 106 mixture at a concentration of 0.9 ppm was compared. The respective amount of PCBs removed from the fungal cultures ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02898723
更新日期：1997-01-01 00:00:00
abstract：:The bacterial strain KDF8 capable of growth in the presence of diclofenac and codeine analgesics was obtained after chemical mutagenesis of nature isolates from polluted soils. The strain KDF8 was identified as Raoultella sp. based on its morphology, biochemical properties, and 16S rRNA gene sequence. It was deposited...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-017-0563-2
更新日期：2018-05-01 00:00:00
abstract：:Rabbits, sensitized with M. kansasii, responded by a profound inhibition of migration of macrophages elicited by both antigens: the migration index for homologous antigen was 0.51 in the direct test and 0.65 in the indirect test; for heterologous antigen the indexes were 0.53 and 0.67. However, significant differences...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02877345
更新日期：1980-01-01 00:00:00
abstract：:NaN3 was found to inhibit transformation but not the irreversible binding of donor 3H-DNA in competent cells of the original low-transformable strain Bacillus subtilis 168 trp2. Addition of NaN3 to cells of two mutants Bacillus subtilis HT39 and HT46 with an increased transformability decreased substantially the irrev...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02877669
更新日期：1977-01-01 00:00:00
abstract：:In the present study, the cDNA sequences of Hsp70 and Hsp90 genes of Isaria farinosa (designated IFHSP70 and IFHSP90) were cloned and characterized using multiple techniques of molecular biology and bioinformatics. The genetic differentiation of the two genes was investigated among 10 geographically separated populati...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-018-00676-x
更新日期：2019-07-01 00:00:00
abstract：:Acmycin, a new polyene antibiotic, was fungistatic at low and fungicidal at high concentrations. It produced malformations in conidia of Helminthosporium oryzae and Curvularia lunata and caused leakage of yeast cells. Acmycin was fairly nontoxic to germinating seeds. The intravenous LD50 was 300-400 and intraperitonea...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02879559
更新日期：1983-01-01 00:00:00
abstract：:Sodium fluoride showed pH-dependent physiological responses in the two test microalgae Anabaena khannae and Chlorococcum humicola. A. khannae showed severe membrane damage with fluoride at low pH with leakage of pigments and electrolytes. Annihilation of photosynthesis along with inhibition in 14C uptake was observed ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931045
更新日期：2004-01-01 00:00:00
abstract：:Five trisubstituted quinazolones and eight trisubstituted quinazoline-4-thiones have been tested for antibacterial effects by a microdilution method. Four derivatives exerted a significant effect on E. coli, P. aeruginosa, S. aureus and B. subtilis (IC50 < 100 mg/L). In the bacterium P. aeruginosa six quinazolines sho...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02817411
更新日期：2000-01-01 00:00:00
abstract：:The resistance to killing by free radicals of two mutants ofPhaffia rhodozyma was determined. Mutant 5-7 did not produce astaxanthin but produced beta-carotene, while mutant 3-4 did not produce any carotenoid pigments. The resistance of mutant 5-7 was the same as that of the wild type but mutant 3-4 was rapidly killed...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02816505
更新日期：1998-01-01 00:00:00
abstract：:The geographical distribution of Bacillus anthracis strains and isolates bearing some of the same genetic markers as the Amerithrax Ames isolate was examined and evaluated. At least one mechanism for the horizontal movement of genetic markers was shown amongst isolates and closely related species and the effect of suc...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-008-0074-2
更新日期：2008-01-01 00:00:00
abstract：:The aim of this study was to determine the prevalence of Listeria sp. and Listeria monocytogenes in soil samples with reference to type of fertilizers (natural and artificial) and distance from places intensively exploited by men, as well as to determine the relationship between the presence of L. monocytogenes in the...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-013-0260-8
更新日期：2014-01-01 00:00:00
abstract：:The mathematical theory of substrate-induced acidification by microorganisms was based on special formulation of mass and charge conservation laws for distinct species. The strength of the theory was tested on the ethanol-induced acidification by Candida utilis in pure water and in phthalate buffer. We showed that the...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02836618
更新日期：1992-01-01 00:00:00
abstract：:Cultures of 33 basidiomycete strains out of 35 tested were viable with unchanged characteristics after four years of maintenance on perlite in cryovials. These cultures can be a good substitute for agar cultures in long-term maintenance of fungi. For comparison, the storage under oil was evaluated but it turned out to...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-008-0084-0
更新日期：2008-01-01 00:00:00
abstract：:Vinasse, a recalcitrant waste of the ethanol industry was employed for the production of polyhydroxyalkanoate (PHA) by the extremely halophilic archaeon, Haloarcula marismortui in shake flasks. The PHA was recovered by osmotic lysis of the cells and subsequent purification by sodium hypochlorite and organic solvents. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-011-0092-3
更新日期：2012-01-01 00:00:00
abstract：:Chemical and microscopic features of wood decay by the basidiomycete Coriolopsis occidentalis are described. The fungus was grown on blocks of poplar, oak, and fir wood and caused significant mass, lignin, and saccharide losses in all kinds of wood. Poplar wood was particularly strongly affected. Twelve weeks after in...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814527
更新日期：1994-01-01 00:00:00
abstract：:In this report, Gluconobacter strains were screened for coenzyme Q10 (CoQ10) production. A thermotolerant strain, Gluconobacter japonicus FM10, was eventually employed for CoQ10 production optimization. To do so, a two-step optimization strategy was used. The first step focused on biomass increase and the second step ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-018-0666-4
更新日期：2019-07-01 00:00:00
abstract：:Streptomyces caelestis DSM 40084 produces two osmolytes, viz. 2-O-(alpha-D-glucopyranosyl)-zeta-glyceric acid (GG) and trehalose. Both compounds were isolated and identified by nuclear magnetic resonance spectroscopy and mass spectrometry. A very sensitive regulation of the cell osmolytes was demonstrated in exponenti...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02932103
更新日期：2007-01-01 00:00:00
abstract：:A small-colony forming variant of Escherichia coli with a mutation in the ncf gene was analysed. The alternation of the protein composition in the cytoplasmic membrane and the interaction with K and E group colicins indicated a membrane mutation. The effect of this mutation on some membrane-bound processes, the activi...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02885601
更新日期：1977-01-01 00:00:00
abstract：:The pathogenesis of bacillary dysentery can be studied at different levels of integration of the cellular components that constitute the colonic mucosal barrier. We considered the interaction of Shigella flexneri in three experimental systems that provide complementary information and a scheme of events occurring in h...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/BF02818608
更新日期：1998-01-01 00:00:00
abstract：:Using fermentation with a selected strain of Acetomonas oxydans it was possible to convert 2-deoxy-2-fluro-D-glucitol to 5-deoxy-5-fluoro-L-sorbose, in agreement with Bertrand's and Hudson's rule. The last-named compound was isolated in a yield of 88%. Both compounds were little toxic against Acetomonas oxydans. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02877659
更新日期：1977-01-01 00:00:00
abstract：:An economic method for a rapid estimation of the number of copies of plasmid R6K delta 1 in E. coli cells using lysis of the cells directly on the agarose gel and electrophoretic separation of radioactively labelled plasmid and chromosomal DNA's is described. The method, particularly useful for screening purposes, per...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02879773
更新日期：1982-01-01 00:00:00
abstract：:This is the first report describing precipitation of bacteriophage particles with zinc chloride as a method of choice to isolate infectious lytic bacteriophages against Pectobacterium spp. and Dickeya spp. from environmental samples. The isolated bacteriophages are ready to use to study various (ecological) aspects of...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-015-0411-1
更新日期：2016-01-01 00:00:00
abstract：:A feeding study was performed to monitor the effect of chitosan intake on the fecal microbiota of ten healthy human subjects. Diversity of microflora was monitored during 8 weeks including 4 weeks of chitosan supplementations. Using denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA gene amplicons and...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-010-0057-y
更新日期：2010-07-01 00:00:00
abstract：:The prevalence of potential virulence markers was determined among the population of Polish Campylobacter jejuni and Campylobacter coli isolates from children, chickens, pigs and dogs. The presence of the flaA, flaB, cdtA, cdtB, cdtC, cdtABC, virB11, and cj0588 genes among 74 C. jejuni and 15 C. coli isolates was dete...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-010-0025-6
更新日期：2010-03-01 00:00:00
abstract：:Using mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine, a number of homoserine auxotrophs have been isolated from a glutamate-producing Arthrobacter globiformis excreting L-lysine in good amounts. For further improvement, mutants resistant to the lysine analog S-(2-aminoethyl)-L-cysteine have been isolated from h...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02935823
更新日期：1991-01-01 00:00:00
abstract：:Infiltration of immune cells into CNS is one of the essential events in multiple sclerosis (MS) development. Adhesion molecules like the intercellular adhesion molecule 1 (ICAM-1) play critical role in this process. Therefore, the ICAM1 gene containing two important single-nucleotide polymorphisms (SNPs) belongs to ca...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-017-0499-6
更新日期：2017-07-01 00:00:00
