Abstract:
:The pathogenesis of bacillary dysentery can be studied at different levels of integration of the cellular components that constitute the colonic mucosal barrier. We considered the interaction of Shigella flexneri in three experimental systems that provide complementary information and a scheme of events occurring in human colorectal mucosa as Shigella invasion proceeds. Interaction of S. flexneri with individual epithelial cells shows a series of events in which the bacterium, upon contact with the cell surface, releases a set of Ipa proteins (i.e. invasins) through a specialized, activable, type-III secretory apparatus (i.e. Mxi/Spa). Via a complex signaling process, these invasins cause major rearrangements of the subcortical cytoskeletal network which allow bacterial entry by a macropinocytotic event. Then the bacterium lyses its phagocytotic vacuole and initiates intracytoplasmic movement, due to polar assembly of actin filaments caused by a bacterial surface protein, IcsA. This allows very efficient colonization of the host cell cytoplasm and passage to adjacent cells via protrusions which are engulfed by a cadherin-dependent process. However, when invasive Shigella are deposited on the apical side of polarized monolayers of human colonic cells, they appear unable to invade, indicating that bacteria need to reach the subepithelial area to invade the epithelium. In this system, it has been shown that transepithelial signaling caused by apical bacteria induces adherence and transmigration of basal polymorphonuclears (PMN), thus disrupting the monolayer permeability and facilitating bacterial invasion. LPS accounts for a large part of this transepithelial signalization to PMN. Such a process could account for invasion in intestinal crypts. Finally, models of infection, such as the rabbit ligated intestinal loop show that initial bacterial entry occurs essentially via M cells of the follicular associated epithelium. It then causes apoptosis of macrophages located in the follicular dome, inducing release of IL-1 beta which, in turn, initiates inflammation, leading to destabilization of the epithelial structures as modeled above. These data can now be used to understand the mechanisms of mucosal protection against bacillary dysentery.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Sansonetti PJdoi
10.1007/BF02818608subject
Has Abstractpub_date
1998-01-01 00:00:00pages
239-46issue
3eissn
0015-5632issn
1874-9356journal_volume
43pub_type
杂志文章,评审abstract::A total of 40 substances were tested for their inhibitory effect on the multiplication of a bacteriophage in a growing culture of Bacillus licheniformis and their influence on bacitracin production. Acriflavine was the only substance which, at a concentration of 3 microgram ml-1, completely suppressed phage multiplica...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02876597
更新日期:1978-01-01 00:00:00
abstract::A small-colony forming variant of Escherichia coli with a mutation in the ncf gene was analysed. The alternation of the protein composition in the cytoplasmic membrane and the interaction with K and E group colicins indicated a membrane mutation. The effect of this mutation on some membrane-bound processes, the activi...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02885601
更新日期:1977-01-01 00:00:00
abstract::Vibrio el tor cells contain a constitutive reductase enzyme which converts nitrofurantoin to an active principle that is responsible for the observed antibacterial activity of the drug. Acquisition of resistance of this strain towards nitrofurantoin is associated with the loss of this reductase. This enzyme is located...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02927181
更新日期:1979-01-01 00:00:00
abstract::This study presents the effects of the probiotic and enterocin M-producing strain Enterococcus faecium AL41 on microbiota, phagocytic activity (PA), oxidative stress, performance and biochemical parameters when applied individually or in combination with Eleutherococcus senticosus in rabbits. The novelty of the study ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-015-0423-x
更新日期:2016-03-01 00:00:00
abstract::The synthesis of exocellular proteinase decreases with increasing concentration of amino acids in the growth medium. After removal of amino acids the enzyme synthesis is gradually restored to normal values. The presence of inhibitors of transcription (actinomycin D) or translation (chloramphenicol) blocks the restorat...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02875957
更新日期:1984-01-01 00:00:00
abstract::The synergistic anticandidal activity of three diketopiperazines [cyclo-(L-Pro-L-Leu) (1), cyclo-(D-Pro-L-Leu) (2), and cyclo-(D-Pro-L-Tyr) (3)] purified from a Bacillus sp. N strain associated with entomopathogenic nematode Rhabditis (Oscheius) in combination with amphotericin B and clotrimazole was investigated usin...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-013-0234-x
更新日期:2013-11-01 00:00:00
abstract::Thirty-three fully breast-fed infants aged between 1 and 12 weeks were screened for bifidobacteria in feces. Bifidobacteria counts in most fecal samples determined both by TPY agar and FISH procedure ranged from 10(8) to 10(11) CFU/g. Three infants did not contain any bifidobacteria in their fecal samples. One child w...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931404
更新日期:2004-01-01 00:00:00
abstract::Fourteen of 200 Lactobacillus isolates from African fermented foods, viz. 'wara', 'kenkey', 'ugba', 'ogi', 'kunuzarki', 'fufu' and 'iru' were found to produce bacteriocins against L. plantarum and only three bacteriocinogenic isolates inhibited some of the food pathogens. Plasmid analysis of the 14 bacteriocin-produci...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02814645
更新日期:1994-01-01 00:00:00
abstract::Dithiocarbamates were found to enhance the production of citric acid under solid-state fermentation conditions by Aspergillus niger. Maximum increase was observed with tetramethylthiuram disulfide (TMTD). Percent increases observed were 74.2% with 2.5 micrograms/mL of TMTD, 19.6% with 2.5 micrograms/mL of sodium dimet...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02926953
更新日期:1986-01-01 00:00:00
abstract::Using mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine, a number of homoserine auxotrophs have been isolated from a glutamate-producing Arthrobacter globiformis excreting L-lysine in good amounts. For further improvement, mutants resistant to the lysine analog S-(2-aminoethyl)-L-cysteine have been isolated from h...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02935823
更新日期:1991-01-01 00:00:00
abstract::The present study was designed to characterize phenotypically and genotypically a Trueperella pyogenes strain isolated from a brain abscess of an adult roebuck (Capreolus capreolus). The species identity could be confirmed by phenotypical investigations, by MALDI-TOF MS analysis, and by sequencing the 16S ribosomal RN...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-017-0533-8
更新日期:2018-01-01 00:00:00
abstract::In this study, a method combining Raman spectroscopy with chemometric analysis was developed for detection of phage presence in raw milk and discrimination of Streptococcus thermophilus and Lactobacillus bulgaricus phages which are among the main phages causing problems in dairy industry. For this purpose, S. thermoph...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-018-0604-5
更新日期:2018-09-01 00:00:00
abstract::Mathematical expressions were derived for the dilution rate and concentration of algae determining the maximum biomass production in a semicontinuous algal culture. ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02926655
更新日期:1979-01-01 00:00:00
abstract::Some Bacillus subtilis mutants with different levels of homoserine dehydrogenase were described. Strains that do not accumulate methionine have a high homoserine dehydrogenase activity. Low activity was detected in mutants where cell growth was completely inhibited by 0.7 mmol/L methionine. A low concentration of dime...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02884064
更新日期:1991-01-01 00:00:00
abstract::Cord and newborn sera were found to possess a significantly decreased in vitro bactericidal activity to Escherichia coli as compared to sera of adult individuals. Hyperbilirubinaemia in neonates suffering from both Rh and ABO incompatibility results in a further significant impairment of the bactericidal activity of t...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02927298
更新日期:1979-01-01 00:00:00
abstract::In Slovakia, dairy products made from ewes' milk have a long tradition. These products include the lactic acid product called "žinčica" which is a by-product occurring during the preparation of ewes' lump cheese. There is no information in the literature regarding the special properties of the microbiota, especially l...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-019-00703-5
更新日期:2020-02-01 00:00:00
abstract::Inhibition of methanogenesis in ruminal cultures was attempted by hindering thiamine availability through its degradation by 'polyphenols' and competition for active sites on enzymes and transporters using thiamine structural analogs. Effects on fermentation were small and not consistently reversed by adding thiamine....
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-009-0075-9
更新日期:2009-11-01 00:00:00
abstract::The effect of subinhibitory concentrations (subMICs) of new organic ammonium salts of four homologous series of alkylammonium bromides (32 compounds) was determined with respect to the induction of lysogenic strain prophage, influence of permeability reactions in a rabbit skin test and cytotoxic changes of monolayers ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02815414
更新日期:1995-01-01 00:00:00
abstract::When investigating the effect of aeration on the utilization of a lipid substrate from the cultivation broth and production of salinomycin in Streptomyces albus it was demonstrated that a higher aeration results in a better utilization of soya oil and a higher production of the antibiotic. ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02879490
更新日期:1983-01-01 00:00:00
abstract::In yeast, the STB5 gene encodes a transcriptional factor belonging to binuclear cluster class (Zn2Cys6) of transcriptional regulators specific to ascomycetes. In this study, we prepared the Kluyveromyces lactis stb5Δ strain and assessed its responses to different stresses. We showed that KlSTB5 gene is able to complem...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-019-00682-7
更新日期:2019-07-01 00:00:00
abstract::The treatment of three patients suffering from chronic bacterial prostatitis who were qualified for an experimental phage therapy protocol managed at the Phage Therapy Unit in Wrocław is described. They had previously been treated unsuccessfully with long-term targeted antibiotics, autovaccines, and laser biostimulati...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-009-0064-z
更新日期:2009-09-01 00:00:00
abstract::The assumption that the pyruvate decarboxylase activity of Saccharomyces carlsbergensis is the main limiting factor determining the formation rate and the total amount of d(--)-l-hydroxy-l-phenyl-propanone (phenylacetylcarbinol, PAC) produced was not confirmed. An increase of about 30% of the total amount of the PAC p...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02877396
更新日期:1982-01-01 00:00:00
abstract::Overpopulation of domestic pigeons is considered to be one of the major problems of urban centers, as these birds are responsible for the dissemination of relevant pathogens to animal and human health. The aim of this study was to detect potentially pathogenic Escherichia coli and Salmonella spp. in domestic pigeons c...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-020-00781-w
更新日期:2020-08-01 00:00:00
abstract::Antibody response to major porin proteins of S. Typhi (OmpC and OmpF) was evaluated in sera of typhoid patients (culture positive, n = 28; Widal positive, n = 16). Sera from fever patients (n = 6) having etiology other than Salmonella, and normal healthy human controls (n = 18) were also included. No significant diffe...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-010-0086-6
更新日期:2010-09-01 00:00:00
abstract::Bacterial metabolites with communicative functions could provide protection against stress conditions to members of the same species. Yet, information remains limited about protection provided by metabolites in Bacillus cereus and inter-species. This study investigated the effect of extracellular compounds derived fro...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-016-0487-2
更新日期:2017-05-01 00:00:00
abstract::Isolation and structure elucidation was carried out of flavonoid constituents found in fractionated extracts of the seeds and green leaves of Daucus carota L. var. boissieri (Apiaceae). The flavonoids are mainly apigenin, luteolin, their glycosidic precursors and 2,4,5-trimethoxybenzaldehyde. Fatty acids, hydrocarbons...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02903720
更新日期:1999-01-01 00:00:00
abstract::Biofilms represent mixed communities present in a diverse range of environments; however, their utility as inoculants is less investigated. Our investigation was aimed towards in vitro development of biofilms using fungal mycelia (Trichoderma viride) as matrices and nitrogen-fixing and P-solubilizing bacteria as partn...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-012-0154-1
更新日期:2012-09-01 00:00:00
abstract::The regulation of gene transcription allows yeast cells to respond properly to changing environmental conditions. Several protein complexes take part in this process. They involve RNA polymerase complexes, chromatin remodeling complexes, mediators, general transcription factors and specific transcriptional regulators....
journal_title:Folia microbiologica
pub_type: 杂志文章,评审
doi:10.1007/s12223-008-0044-8
更新日期:2008-01-01 00:00:00
abstract::A total of 152 lactic acid bacteria (LAB) were isolated from pickles produced in the Ankara-Çubuk region. These isolates were clustered into eight groups on the basis of their phenotypic characteristics including cell morphology, CO2 production from glucose, growth at 10 and 45 °C, growth in 6.5 % NaCl, and growth at ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-014-0363-x
更新日期:2015-05-01 00:00:00
abstract::Data on the production of clostridial toxins in dialyzed cultures are summarized. Principal modifications of this cultivation technique suitable for both research and production are shown. If toxins are released from the cells by autolysis (neurotoxins of Clostridium tetani, C. botulinum; lethal factors of C. novyi an...
journal_title:Folia microbiologica
pub_type: 杂志文章,评审
doi:10.1007/BF02928083
更新日期:1988-01-01 00:00:00