Abstract:
:The prevalence of potential virulence markers was determined among the population of Polish Campylobacter jejuni and Campylobacter coli isolates from children, chickens, pigs and dogs. The presence of the flaA, flaB, cdtA, cdtB, cdtC, cdtABC, virB11, and cj0588 genes among 74 C. jejuni and 15 C. coli isolates was detected by PCR. High prevalence of five different putative virulence and toxin genes (flaA, cdtA, cdtB, cdtC, and cj0588) was found among isolates obtained from children, chickens and dogs. The occurrence of these genes among isolates obtained from pigs was significantly different than for strains isolated from other sources. Two methods for genotyping Campylobacter spp. strains were applied - flaA-typing, and ADSRRS-fingerprinting method, which was used for the first time for Campylobacter spp. strains. Similarity of the genetic profiles was demonstrated in strains isolated from chickens and dogs, and in isolates from chickens and children. Strains isolated from pigs, both C. jejuni as well as C. coli, did not group with isolates from other sources.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Krutkiewicz A,Klimuszko Ddoi
10.1007/s12223-010-0025-6subject
Has Abstractpub_date
2010-03-01 00:00:00pages
167-75issue
2eissn
0015-5632issn
1874-9356journal_volume
55pub_type
杂志文章abstract::Anaerobic ammonium-oxidizing (anammox) bacteria play an essential part in nitrogen removal in constructed wetlands. The objective of the present study was to explore the spatiotemporal dynamics of anammox bacterial communities and the associated factors in a full-scale constructed wetland for the treatment of polluted...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-019-00726-y
更新日期:2020-04-01 00:00:00
abstract::Frequency of heterocytes and nitrogenase activity (NA) under light and dark cultivation conditions was determined in 12 cyanobacterial strains isolated from various soil habitats. In spite of a high variability, significant differences in NA among the strains were found in response of light and dark cultivation. Relat...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931606
更新日期:2004-01-01 00:00:00
abstract::The present study was designed to characterize six Trueperella (T.) abortisuis strains, cultured over a period of 5 months from fetus and abortion material of six pigs of a single farm in Mecklenburg-West Pomerania federal state, Germany. It was of interest to investigate the epidemiological relationships of the six s...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-019-00753-9
更新日期:2020-06-01 00:00:00
abstract::A series of experiments were performed with P. aeruginosa to demonstrate which of the biochemical mechanisms are responsible for the resistance to the beta-lactam antibiotics. The constitutive beta-lactamase was isolated and characterized for the strain used as type OXA whose pI was 7.1, with a molar mass of 49 kg/mol...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02877216
更新日期:1987-01-01 00:00:00
abstract::A collection of 34 lactococcal strains were characterized using the polymerase chain reaction (PCR) for the acmA gene, and for the 16S rDNA gene, and DNA fingerprinting methods for randomly amplified polymorphic DNA (RAPD) and repetitive extragenic palindrome-PCR (rep-PCR). PCR experiments corroborated the genotypic i...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931464
更新日期:2005-01-01 00:00:00
abstract::A wild-type strain of Candida albicans (S1, ATCC 10261) was used to obtain stable auxotrophic colony morphological mutants (mutant M5 producing only true hyphae and mutant M2 containing 90 % blastospores and 10 % pseudohyphae) by induced mutagenesis. A hybrid was produced by somatic hybridization between these 2 mutan...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931528
更新日期:2004-01-01 00:00:00
abstract::Composition of sterol fractions of nystatin-resistant Candida maltosa strains was determined. Using UV-spectrometry, TLC and GLC-MS it was demonstrated that resistance to nystatin is connected with the composition alterations of yeast cell sterols. Block of different stages of ergosterol biosynthesis was revealed in s...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02814494
更新日期:1991-01-01 00:00:00
abstract::Bacteria accumulate high amounts of potassium in the cytoplasm. For studying transport of K+ (with 86Rb as a marker) in bacteria (Staphylococcus aureus 17810S), the cells were depleted of the internal K+ pool by a DNP treatment. Kinetics and energetics of 86Rb transport was assayed with glucose as an exogenous energy ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02816378
更新日期:1998-01-01 00:00:00
abstract::Procedures to introduce point mutations, restriction sites and insert or delete DNA fragments are very important tools to study protein function. We describe here two-step PCR-based method for generating single or multiple mutations, insertions and deletions in a small region of the sequence. In the first step, a uniq...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02816213
更新日期:1999-01-01 00:00:00
abstract::Five trisubstituted quinazolones and eight trisubstituted quinazoline-4-thiones have been tested for antibacterial effects by a microdilution method. Four derivatives exerted a significant effect on E. coli, P. aeruginosa, S. aureus and B. subtilis (IC50 < 100 mg/L). In the bacterium P. aeruginosa six quinazolines sho...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02817411
更新日期:2000-01-01 00:00:00
abstract::This work was aimed to develop a multiplex PCR assay to detect infectious agents such as Clavibacter michiganensis subsp. michiganensis, Fusarium sp, Leveillula taurica, and begomoviruses in tomato (Solanum lycopersicum) plants. Specific primer sets of each pathogen were designed based on intergenic ribosomal RNA sequ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-012-0206-6
更新日期:2013-07-01 00:00:00
abstract::The biological activity and the presence of genes sfp and ituD (surfactin and iturin A) among Bacillus strains isolated from the Amazon basin were determined. Bacillus spp. were tested for hemolytic activity and inhibition of fungal growth by agar plate assays in parallel with PCR for identification of sfp and ituD ge...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-011-0056-7
更新日期:2011-07-01 00:00:00
abstract::Verotoxin-producing Escherichia coli (VTEC) are nowadays among the most important emerging group of food-borne pathogens (VTEC strains cause gastroenteritis that can be complicated by the hemorrhagic colitis or hemolytic uremic syndrome, HUS). Escherichia coli 026 producing verotoxin 2 was isolated and its identity co...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931454
更新日期:2005-01-01 00:00:00
abstract::Isolation and structure elucidation was carried out of flavonoid constituents found in fractionated extracts of the seeds and green leaves of Daucus carota L. var. boissieri (Apiaceae). The flavonoids are mainly apigenin, luteolin, their glycosidic precursors and 2,4,5-trimethoxybenzaldehyde. Fatty acids, hydrocarbons...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02903720
更新日期:1999-01-01 00:00:00
abstract::Acmycin, a new polyene antibiotic, was fungistatic at low and fungicidal at high concentrations. It produced malformations in conidia of Helminthosporium oryzae and Curvularia lunata and caused leakage of yeast cells. Acmycin was fairly nontoxic to germinating seeds. The intravenous LD50 was 300-400 and intraperitonea...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02879559
更新日期:1983-01-01 00:00:00
abstract::A small-colony forming variant of Escherichia coli with a mutation in the ncf gene was analysed. The alternation of the protein composition in the cytoplasmic membrane and the interaction with K and E group colicins indicated a membrane mutation. The effect of this mutation on some membrane-bound processes, the activi...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02885601
更新日期:1977-01-01 00:00:00
abstract::We report a novel application of calcein-acetomethyl ester in flow cytometry for rapid estimation of the number of G+-bacteria in rodent feces (Apodemus sylvaticus and Mus sp.f. muridae). We also use the combined application of flow cytometry and Syto-13 or Sypro Orange staining to count rapidly the total bacterial po...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931538
更新日期:2004-01-01 00:00:00
abstract::Virulence factors were compared in 15 Aeromonas spp. isolated from faeces of patients with Aeromonas-associated gastroenteritis and in 81 strains isolated from food. Strains from food did not show differences in the distribution of virulence factors when compared with strains isolated from faeces. However, 88.8% of Ae...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02814323
更新日期:1994-01-01 00:00:00
abstract::The color of light (white, red, blue, and green) had a significant effect on the growth and reproductive processes (both in the nucleocytoplasmic and chloroplast compartment of the cells) in synchronous cultures of Scenedesmus obliquus. This effect decreased in the order red > white > blue > green. In the same order, ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931828
更新日期:2006-01-01 00:00:00
abstract::Two Bacillus cereus feather-degrading strains (23/1 and 6/2) were transformed using a recombinant plasmid p5.2 carrying the alkaline proteinase gene (aprE). A high level of the aprE gene expression was observed when the recombinant strains were grown on sporulation medium. The expression of the aprE gene proceeded dur...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-010-0004-y
更新日期:2010-01-01 00:00:00
abstract::Staphylococcus epidermidis is a biofilm-forming bacterial strain that can cause major problems as an agent of nosocomial infections. Bacteria in biofilms are shielded from the environment and can survive high doses of antibiotics. We here test the antibiotic susceptibility of Staphylococcus epidermidis to rising genta...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-017-0568-x
更新日期:2018-05-01 00:00:00
abstract::The screening of 17 SAg genes of S. aureus isolated from the sputum of cystic fibrosis (CF) patients revealed that among 47 genetically different strains, 39 (83 %) carried SAg genes. Superantigens forming enterotoxin gene cluster were detected in 20 strains. The 2nd most common superantigen type was selk detected in ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931628
更新日期:2006-01-01 00:00:00
abstract::The ribosomal stalk is involved directly in the interaction of the elongation factors with the ribosome during protein synthesis. The stalk is formed by a complex of five proteins, four small acidic polypeptides and a larger protein which directly interacts with the rRNA at the GTPase center. In eukaryotes, the acidic...
journal_title:Folia microbiologica
pub_type: 杂志文章,评审
doi:10.1007/BF02816234
更新日期:1999-01-01 00:00:00
abstract::There is a growing interest in the use of bioinoculants to assist mineral fertilizers in improving crop production and yield. Azotobacter and Pseudomonas are two agriculturally relevant strains of bacteria which have been established as efficient bioinoculants. An experiment involving addition of graded concentrations...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-017-0495-x
更新日期:2017-05-01 00:00:00
abstract::Fibrobacter succinogenes is a major cellulolytic species in the rumen. On the basis of molecular data, this species can be classified into four phylogenetic groups. Recently gathered ecological and physiological data have revealed the importance of this species, particularly phylogenetic group 1, in rumen fiber digest...
journal_title:Folia microbiologica
pub_type: 杂志文章,评审
doi:10.1007/s12223-008-0024-z
更新日期:2008-01-01 00:00:00
abstract::Physiological and biochemical properties were tested in 45 isolates of Aspergillus egyptiacus (16 isolates), Emericella nidulans (16) and Aspergillus versicolor (13). The three fungal species exhibited common and similar features. The big similarity between A. egyptiacus and E. nidulans was greater than between A. egy...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02814449
更新日期:1994-01-01 00:00:00
abstract::When investigating the effect of aeration on the utilization of a lipid substrate from the cultivation broth and production of salinomycin in Streptomyces albus it was demonstrated that a higher aeration results in a better utilization of soya oil and a higher production of the antibiotic. ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02879490
更新日期:1983-01-01 00:00:00
abstract::Ecosystems worldwide are exposed to pollutants connected to the industrial production of pharmaceuticals. The objective of this study was to study the composition and characteristics of the soil microbial communities that had been exposed to long-term selection pressure caused by the industrial production of penicilli...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-020-00819-z
更新日期:2020-12-01 00:00:00
abstract::Small plasmid pKST23 was isolated from sheep ruminal Escherichia coli population. Plasmid sequence was determined to be 2,779 bp in length and was found to have an overall 42 % of GC pairs. However, its sequence can be divided into two regions based on genetic composition and the GC content. It was found that the high...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-012-0124-7
更新日期:2012-07-01 00:00:00
abstract::Asymptomatic uropathogenic Escherichia coli (UPECs) are the leading cause of asymptomatic bacteriuria (ABU) in humans. So this study aimed to identify and characterize ABU UPECs from hospitalized patients of Kolkata, India, with respect to their antibiogram profile, phylogeny, pathogenicity islands, and virulence fact...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-019-00685-4
更新日期:2019-07-01 00:00:00