Abstract:
:In the present study, the cDNA sequences of Hsp70 and Hsp90 genes of Isaria farinosa (designated IFHSP70 and IFHSP90) were cloned and characterized using multiple techniques of molecular biology and bioinformatics. The genetic differentiation of the two genes was investigated among 10 geographically separated populations distributed in the Yunnan province. The complete sequence of the IFHSP70 cDNA had a length of 2158 bp, and contained an open reading frame (ORF) of 1962 bp, encoding a 71-kDa polypeptide comprising of 653 amino acids. IFHSP90 cDNA had a length of 2144 bp, and contained an ORF of 2103 bp, encoding a polypeptide of 79.23 kDa, comprising of 700 amino acids. The deduced amino acid sequences of IFHSP70 and IFHSP90 shared high sequence identities with other fungi. Fundamental information pertaining to the protein families, signatures, and conserved motifs of Hsp70 and Hsp90 were also identified. Analysis of molecular variances (AMOVA) from the Hsp70 and Hsp90 genes showed that the genetic variation within-population (83.26%, 83.08%) was greater than among the populations (16.74%, 16.92%). The values of nucleotide diversity (Pi), haplotype diversity (Hd), coefficient of genetic differentiation (Fst), and gene flow (Nm) were calculated. For Hsp70, Pi = 0.0425, Hd = 0.888, Fst = 0.167, Nm = 1.24; For Hsp90, Pi = 0.0420, Hd = 0.894, Fst = 0.169, and Nm = 1.22. These data indicated that the genetic differentiation among 10 different geographical populations of I. farinosa was limited. This study describes, for the first time, cloning, characterization and identification of Isaria farinosa Hsp70 and Hsp90 genes, and provides a preliminary basis for exploring the genetic structure of the genus Isaria using the sequences of Hsp70 and Hsp90 as molecular markers.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
He L,Yu H,Dai Y,Wang Ydoi
10.1007/s12223-018-00676-xsubject
Has Abstractpub_date
2019-07-01 00:00:00pages
521-534issue
4eissn
0015-5632issn
1874-9356pii
10.1007/s12223-018-00676-xjournal_volume
64pub_type
杂志文章abstract::Bacterial metabolites with communicative functions could provide protection against stress conditions to members of the same species. Yet, information remains limited about protection provided by metabolites in Bacillus cereus and inter-species. This study investigated the effect of extracellular compounds derived fro...
journal_title:Folia microbiologica
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journal_title:Folia microbiologica
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pub_type: 杂志文章
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更新日期:2010-05-01 00:00:00
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journal_title:Folia microbiologica
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journal_title:Folia microbiologica
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doi:10.1007/BF02876583
更新日期:1978-01-01 00:00:00
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journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-015-0411-1
更新日期:2016-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/BF02818710
更新日期:2002-01-01 00:00:00
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journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02932103
更新日期:2007-01-01 00:00:00
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journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-010-0047-0
更新日期:2010-07-01 00:00:00
abstract::The plasmid R1drd-19 Km- and its derivative pON5300 were split by the PstI restriction enzyme; they differed in a 2.4 Mg/mol fragment which was present only in the pON5300. Both plasmids were present in 1-2 copies per chromosomal equivalent in E. coli JC5455 cells. The pON5300 is stable in its original mutagenized hos...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02879554
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journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-012-0165-y
更新日期:2012-11-01 00:00:00
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journal_title:Folia microbiologica
pub_type: 杂志文章
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更新日期:2008-01-01 00:00:00
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journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02932132
更新日期:2007-01-01 00:00:00
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更新日期:1988-01-01 00:00:00
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journal_title:Folia microbiologica
pub_type: 杂志文章
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更新日期:1980-01-01 00:00:00
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journal_title:Folia microbiologica
pub_type: 杂志文章,评审
doi:10.1007/BF02816234
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journal_title:Folia microbiologica
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journal_title:Folia microbiologica
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journal_title:Folia microbiologica
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更新日期:1980-01-01 00:00:00
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journal_title:Folia microbiologica
pub_type: 杂志文章
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更新日期:2018-11-01 00:00:00
abstract::For the first time, it was reported in France a cluster of autochthonous severe community-acquired (CA) infections due to the USA300 methicillin-resistant Staphylococcus aureus (MRSA) clone. The three cases belonged to the same family without any identified clue of abroad importation pathway. The domestic spread of US...
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更新日期:2014-11-01 00:00:00