Production of alkaloids and differentiation in a submerged culture of Claviceps purpurea (Fr.) Tul.

Abstract:

:The submerged culture of the Claviceps purpurea strain studied was polymorphous. In the process of alkaloid synthesis, cytodifferentiation preceded biochemical differentiation. The onset of the alkaloid phase was characterized by: predominance of chlamydospores in the culture, the presence of vegetative cells with reduced or arrested proliferation, maximum acetylCoA carboxylase activity, the maximum amount of total fatty acids, an over-average cell pool tryptophan level and minimum tryptophan synthetase activity. Intracellular ricinoleic acid was an indicator of differentiation of the culture towards alkaloid formation and also of alkaloid synthesis. The cytodifferentiation period in the initial phases of fermentation, when the cell has several alternative possibilities of development, is regarded as the most sensitive sector of the regulatory mechanisms of alkaloid formation.

journal_title

Folia microbiologica

authors

Rehácek Z,Kozová J

doi

10.1007/BF02876766

subject

Has Abstract

pub_date

1975-01-01 00:00:00

pages

112-7

issue

2

eissn

0015-5632

issn

1874-9356

journal_volume

20

pub_type

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