Abstract：
:Ecosystems worldwide are exposed to pollutants connected to the industrial production of pharmaceuticals. The objective of this study was to study the composition and characteristics of the soil microbial communities that had been exposed to long-term selection pressure caused by the industrial production of penicillin G. Soil samples from four sites among the penicillin G production plant were analysed using 16S rRNA profiling via Illumina MiSeq platform and were compared with the control samples from four sites outside the plant. Total metagenomic DNA from the impacted soil was also used for the preparation of E. coli T1R-based fosmid library which was consequently qualitatively tested for the presence of penicillin G acylase (PGA)-encoding genes using the method of sequence homology. Analyses of alpha diversity revealed that the long-term antibiotic presence in the soil significantly increased the microbial diversity and richness in terms of Shannon diversity index (p = 0.002) and Chao estimates (p = 0.004). Principal component analysis showed that the two types of communities (on-site and control) could be separated at the phylum, class and genus level. The on-site soil was enriched in Betaproteobacteria, Deltaproteobacteria, Gemmatimonadetes, Acidobacteria and Planctomycetia, while a significant decrease in Actinobacteria was observed. Metagenomic fosmid library revealed high hit rates in identifying PGAs (14 different genes identified) and confirmed the biotechnological potential of soils impacted by anthropogenic activity. This study offers new insights into the changes in microbial communities of soils exposed to anthropogenic activity as well as indicates that those soils may represent a hotspot for biotechnologically interesting targets.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Borčinová M,Pitkina A,Marešová H,Štěpánek V,Palyzová A,Kyslík Pdoi
10.1007/s12223-020-00819-zsubject
Has Abstractpub_date
2020-12-01 00:00:00pages
1061-1072issue
6eissn
0015-5632issn
1874-9356pii
10.1007/s12223-020-00819-zjournal_volume
65pub_type
杂志文章abstract：:Acmycin, a new polyene antibiotic, was fungistatic at low and fungicidal at high concentrations. It produced malformations in conidia of Helminthosporium oryzae and Curvularia lunata and caused leakage of yeast cells. Acmycin was fairly nontoxic to germinating seeds. The intravenous LD50 was 300-400 and intraperitonea...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02879559
更新日期：1983-01-01 00:00:00
abstract：:Euglena ignobilis cells in natural puddle water of pH 7.8, when kept at 21 +/- 2 degrees C and under continuous light (intensity of approximately 30 micromol m(-2) s(-1)) in a culture chamber, decreased their speed of movement from > or = 78000 microm/min (after a 12-h cultivation), to 850-1300 microm/min after 18 h. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931412
更新日期：2005-01-01 00:00:00
abstract：:An extracellular acid phosphatase isolated from the culture of a wild strain Aspergillus niger, producing the dephosphorylating 3-phytase, was obtained in a homogeneous form by sequential application of ultrafiltration through PS 50 membrane, gel filtration on Sephadex G-100 and ion exchange chromatography on DEAE-Sep...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931618
更新日期：2006-01-01 00:00:00
abstract：:Although humic acids (HA) are involved in many biological processes in soils and thus their ecological importance has received much attention, the degradative pathways and corresponding catalytic genes underlying the HA degradation by bacteria remain unclear. To unveil those uncertainties, we analyzed transcriptomes e...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-017-0573-0
更新日期：2018-05-01 00:00:00
abstract：:A new metabolite was isolated from the culture of Penicillium rugulosum and its structure was determined from physico-chemical data. Accompanying metabolites skyrin and rugulosin were characterized by UV, IR, CD, mass and NMR spectra. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02876598
更新日期：1978-01-01 00:00:00
abstract：:The structural gene coding for YompC has been identified in the genome of a pathogenic strain of Yersinia enterocolitica O:9, and was subsequently cloned and sequenced. Detailed alignment of the deduced amino acid sequence showed that YompC is a member of the OmpC porin family with the highest degree of homology to Kl...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02932142
更新日期：2007-01-01 00:00:00
abstract：:As a part of a program to develop starter cultures aiding in the spoilage control and sanitation of African fermented foods, a cereal-based food ('ogi' and its solid form 'agidi' or 'eko') was prepared using a bacteriocin-producing Lactobacillus strain as the starter culture. The survival of an enterotoxigenic Escheri...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02898642
更新日期：1997-01-01 00:00:00
abstract：:Three indigenous plasmids designated pRK1, pRK2 and pRK3 were identified among producers of penicillin G acylase (PGA) derived from the strain Escherichia coli W ATCC 9637. Their size and copy number (CN) in E. coli W were determined (kb; CN): pRK1 (80; 3.4), pRK2 (5.1; 71), and pRK3 (4.8; 13.7). Strain E. coli RE2 ha...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02818544
更新日期：1999-01-01 00:00:00
abstract：:We have investigated the expression of cyclins, cyclin dependent kinases (CDK), and CDK inhibitors (CKI) at the mRNA level in a panel of small-cell lung cancer (SCLC) cell lines in vitro and in vivo as xenografts in nude mice. The results showed that the cell lines expressed varying amounts of most cyclin and CDK's bu...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02820804
更新日期：1998-01-01 00:00:00
abstract：:Streptococcus dysgalactiae subsp. equisimilis (SDSE), belonging to the group C and G streptococci, are human pathogens reported to cause clinical manifestations similar to infections caused by Streptococcus pyogenes. To scrutinize the distribution of gene coding for S. pyogenes virulence factors in SDSE, 255 isolates ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-018-0595-2
更新日期：2018-09-01 00:00:00
abstract：:The effect of dental plaque bacteria on LFA-1 beta expression on peripheral blood leukocytes was studied in 20 patients with early-onset periodontitis and in 10 healthy controls. Stimulation of PMN with selected dental plaque bacteria which play a role in the pathogeny of periodontitis significantly increased the expr...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02815697
更新日期：1996-01-01 00:00:00
abstract：:Lactobacillus pobuzihii is a novel species which has been previously found in pobuzihi (fermented cummingcordia), a traditional fermented food in Taiwan. However, the lactic acid bacteria (LAB) microflora in pobuzihi has not been studied in detail. In this study, LAB from pobuzihi were isolated, identified, and charac...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-012-0188-4
更新日期：2013-03-01 00:00:00
abstract：:The reserves of fossil-based fuels, which currently seem sufficient to meet the global demands, is inevitably on the verge of exhaustion. Contemporary raw material for alternate fuel like biodiesel is usually edible plant commodity oils, whose increasing public consumption rate raises the need of finding a non-edible ...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/s12223-018-0602-7
更新日期：2018-09-01 00:00:00
abstract：:The sorption ability of Candida utilis biomass for cadmium ions with accumulating competence of dried cells and cells in alginate was compared. After an optimization of process conditions (pH 5.5, biomass concentration 1 g/L and c0 50 mg/L), the cadmium sorption capacity of dried yeast biomass was perceptibly higher t...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931807
更新日期：2006-01-01 00:00:00
abstract：:Moderately halophilic bacteria utilizing phenol as the sole carbon source were isolated by selective enrichment from sea water. The isolate (Gram-negative motile rods) was identified as Deleya venusta. It grew well in the presence of up to 1600 mg/L of phenol and 8% NaCl under aerobic conditions. When the cells were t...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02815617
更新日期：2001-01-01 00:00:00
abstract：:Pullulanase of Klebsiella oxytoca is one of a wide variety of extracellular proteins that are secreted by Gram-negative bacteria by the complex main terminal branch (MTB) of the general secretory pathway. The roles of some of the 14 components of the MTB are now becoming clear. In this review it is proposed that most ...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/BF02818976
更新日期：1997-01-01 00:00:00
abstract：:Aliphatic 1,2-alkanolamines inhibited beta-glucanase from Candida utilis. The highest inhibitory effect was observed with 1-dodecylamino-3-chloro-2-propanol (Ki = 1.0 mumol/L) and it was much higher than that of D-glucono-delta-lactone. Simple 1,2-alkanolamines, like 2-aminoethanol or 2-methylaminoethanol, did not exh...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02898763
更新日期：1993-01-01 00:00:00
abstract：:The effect of the newly synthesized phosphonic compound dibutyl 2-octylamino-2-propanephosphonate (DBOP) on the growth of the aquatic plant Spirodela oligorrhiza and stability of red blood cells (RBC) and planar lipid membranes (BLM) was studied to determine its physiological activity and, if possible, correlate this ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02908944
更新日期：2000-01-01 00:00:00
abstract：:We isolated and identified functional groups of bacteria in the rumen of Creole goats involved in ruminal fermentation of native forage shrubs. The functional bacterial groups were evaluated by comparing the total viable, total anaerobic, cellulolytic, hemicellulolytic, and amylolytic bacterial counts in the samples t...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-012-0219-1
更新日期：2013-09-01 00:00:00
abstract：:The ability of six bifidobacterial strains (3 of human origin and 3 isolates from fermented milk products) to utilize glucose, lactose, melezitose, sucrose, raffinose, and stachyose was determined. Dairy-related bifidobacterial strains were identified as Bifidobacterium animalis (2 strains) or as B. pseudolongum (1 st...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02818784
更新日期：2002-01-01 00:00:00
abstract：:A 6.5 kb DNA fragment containing the gene (thrC) encoding threonine synthase, the last enzyme of the threonine biosynthetic pathway, has been cloned from the DNA of Bacillus sp. ULM1 by complementation of Escherichia coli and Brevibacterium lactofermentum thrC auxotrophs. Complementation studies showed that the thrB g...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02818515
更新日期：1995-01-01 00:00:00
abstract：:Antibody response to major porin proteins of S. Typhi (OmpC and OmpF) was evaluated in sera of typhoid patients (culture positive, n = 28; Widal positive, n = 16). Sera from fever patients (n = 6) having etiology other than Salmonella, and normal healthy human controls (n = 18) were also included. No significant diffe...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-010-0086-6
更新日期：2010-09-01 00:00:00
abstract：:Secreted aspartyl proteinase (Sap) distribution among different C. albicans isolates was determined using SAP-specific primers in polymerase chain reaction (PCR) assay. SAP1, SAP2, and SAP3 were detected in 13 of 40 (32.5%), SAP4 in 38/40 (95%), SAP5 were detected in 30/40 (75%), SAP6 in 23/40 (57.5%) of C. albicans s...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931422
更新日期：2005-01-01 00:00:00
abstract：:Biological activity of secondary metabolites produced by a plant-growth-promoting Pseudomonas fluorescens was evaluated. The strain produced antibiotics phenazine (PHE), 2,4-diacetylphloroglucinol (PHL) and siderophore pyoverdin (PYO) in standard King's B and succinic acid media, respectively. After extraction, PYO wa...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02818690
更新日期：2002-01-01 00:00:00
abstract：:Fourteen of 200 Lactobacillus isolates from African fermented foods, viz. 'wara', 'kenkey', 'ugba', 'ogi', 'kunuzarki', 'fufu' and 'iru' were found to produce bacteriocins against L. plantarum and only three bacteriocinogenic isolates inhibited some of the food pathogens. Plasmid analysis of the 14 bacteriocin-produci...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814645
更新日期：1994-01-01 00:00:00
abstract：:Using fluorescein-labelled antibodies against gamma, mu and alpha chains, Ig-containing cells in palatine tonsils were studied in 120 patients. The aim of this study was to determine the most frequently repeated typical findings as regards the numbers and localisation of these cells in tonsils and to confront the data...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02927432
更新日期：1981-01-01 00:00:00
abstract：:In the present study, the cDNA sequences of Hsp70 and Hsp90 genes of Isaria farinosa (designated IFHSP70 and IFHSP90) were cloned and characterized using multiple techniques of molecular biology and bioinformatics. The genetic differentiation of the two genes was investigated among 10 geographically separated populati...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-018-00676-x
更新日期：2019-07-01 00:00:00
abstract：:Streptomyces aureofaciens glucosidizes 1,2,4-trihydroxy-9,10-anthraquinone (purpurin) added to the cultivation medium to yield the corresponding 2-beta-D-glucoside. The identity of the glucoside was demonstrated by comparing its physico-chemical properties with data of an authentic sample prepared synthetically. A fur...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02873156
更新日期：1984-01-01 00:00:00
abstract：:A collection of 34 lactococcal strains were characterized using the polymerase chain reaction (PCR) for the acmA gene, and for the 16S rDNA gene, and DNA fingerprinting methods for randomly amplified polymorphic DNA (RAPD) and repetitive extragenic palindrome-PCR (rep-PCR). PCR experiments corroborated the genotypic i...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931464
更新日期：2005-01-01 00:00:00
abstract：:Oxidizing and assimilating ability of the yeast Candida utilis 49 was tested with 21 different low-boiling organic compounds which come as components of raw synthetic ethanol. The highest yields of yeast dry weight were obtained with ethanol (72.0%), propanol (48.2%), ethyl acetate (43.4%) and acetic acid (34.2%). To ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02876965
更新日期：1976-01-01 00:00:00
