Abstract：
:Although humic acids (HA) are involved in many biological processes in soils and thus their ecological importance has received much attention, the degradative pathways and corresponding catalytic genes underlying the HA degradation by bacteria remain unclear. To unveil those uncertainties, we analyzed transcriptomes extracted from Pseudomonas sp. PAMC 26793 cells time-dependently induced in the presence of HA in a lab flask. Out of 6288 genes, 299 (microarray) and 585 (RNA-seq) were up-regulated by > 2.0-fold in HA-induced cells, compared with controls. A significant portion (9.7% in microarray and 24.1% in RNA-seq) of these genes are predicted to function in the transport and metabolism of small molecule compounds, which could result from microbial HA degradation. To further identify lignin (a surrogate for HA)-degradative genes, 6288 protein sequences were analyzed against carbohydrate-active enzyme database and a self-curated list of putative lignin degradative genes. Out of 19 genes predicted to function in lignin degradation, several genes encoding laccase, dye-decolorizing peroxidase, vanillate O-demethylase oxygenase and reductase, and biphenyl 2,3-dioxygenase were up-regulated > 2.0-fold in RNA-seq. This induction was further confirmed by qRT-PCR, validating the likely involvement of these genes in the degradation of HA.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Kim D,Park HJ,Sul WJ,Park Hdoi
10.1007/s12223-017-0573-0subject
Has Abstractpub_date
2018-05-01 00:00:00pages
315-323issue
3eissn
0015-5632issn
1874-9356pii
10.1007/s12223-017-0573-0journal_volume
63pub_type
杂志文章abstract：:Moderately halophilic bacteria utilizing phenol as the sole carbon source were isolated by selective enrichment from sea water. The isolate (Gram-negative motile rods) was identified as Deleya venusta. It grew well in the presence of up to 1600 mg/L of phenol and 8% NaCl under aerobic conditions. When the cells were t...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02815617
更新日期：2001-01-01 00:00:00
abstract：:Purification of streptococcal extracellular NAD+ nucleosidase is associated with changes of kinetic properties. A high-molecular weight component is required for a full activity of the enzyme. The component is not produced by bacteria and is present in the Todd-Hewitt cultivation medium, the beef-heart extract serving...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02876396
更新日期：1980-01-01 00:00:00
abstract：:Mathematical expressions were derived for the dilution rate and concentration of algae determining the maximum biomass production in a semicontinuous algal culture. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02926655
更新日期：1979-01-01 00:00:00
abstract：:A strain of Aspergillus giganteus cultivated in a medium with xylan produced two xylanases (xylanase I and II) which were purified to homogeneity. Their molar mass, estimated by SDS-PAGE, were 21 and 24 kDa, respectively. Both enzymes are glycoproteins with 50 degrees C temperature optimum; optimum pH was 6.0-6.5 for ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931639
更新日期：2004-01-01 00:00:00
abstract：:The microbial fuel cells (MFCs) are recognized to be highly effective for the biodegradation of phenol. For isolating the phenol-degrading bacteria, the sample containing 500 mg/L phenol was collected from the MFCs. The strain (WL027) was identified basing on the 16S rRNA gene analysis and phylogenetic analysis as Bac...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-017-0536-5
更新日期：2018-01-01 00:00:00
abstract：:Changes in leukocyte counts and in the gut microflora of laboratory rats irradiated with single whole-body dose of gamma rays (5.0 Gy) were determined. The number of leukocytes was lower especially 1 and 2 weeks after irradiation. A significant decrease in lymphocytes was observed 1 week and in monocytes 1 and 2 weeks...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02818710
更新日期：2002-01-01 00:00:00
abstract：:A naturally deficient thiamine and methionine requiring strain of Bacillus coagulans (Ms 5) accumulates lysine in medium only when exogenous pyridoxine (optimal concentration, 0.1 mu g/ml) are supplied. Threonine exerts an inhibitory effect at higher concentrations but pyridoxine does not. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02877085
更新日期：1975-01-01 00:00:00
abstract：:A total of 368 rotavirus RNA-positive (PAGE) stool samples collected continually during 1992-95 from infants and young children under five years of age hospitalized with acute gastroenteritis were serotyped using an enzyme immunoassay with VP7-specific monoclonal antibodies (ELISA with MAbs) for serotypes G1-G4. The s...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02817421
更新日期：2000-01-01 00:00:00
abstract：:The ability of surfactants obtained from three Lactobacillus acidophilus strains to inhibit Staphylococcus aureus and S. epidermidis biofilms was evaluated. Their influence was determined on bacterial initial adhesion, biofilm formation and dispersal using MTT-reduction assay, confocal laser scanning microscopy and im...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-008-0009-y
更新日期：2008-01-01 00:00:00
abstract：:This study was undertaken to develop a new Gram-staining machine controlled by a micro-controller and to investigate the quality of slides that were stained in the machine. The machine was designed and produced by the authors. It uses standard 220 V AC. Staining, washing, and drying periods are controlled by a timer b...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02818557
更新日期：1999-01-01 00:00:00
abstract：:The present study was conducted to determine the gene responsible for beta-glucosidase (BGL) production and to generate a full-length complementary DNA (cDNA) of one of the putative BGL genes, which showed a significant expression level when Schizophyllum commune KUC9397 was grown in optimized medium. The relative exp...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-016-0484-5
更新日期：2017-05-01 00:00:00
abstract：:The gene encoding the coat protein (CP) of a potato virus Y (PVY) was cloned into expression vector pMPM-A4Omega. PVY CP was expressed in Escherichia coli and the purified recombinant protein was used for raising rabbit polyclonal antibodies. The sera and antibodies were tested for the detection of PVY in the laborato...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-008-0067-1
更新日期：2008-01-01 00:00:00
abstract：:Using simultaneous fusion of protoplasts of three strains the auxotrophic K3 killer designated MH1 was prepared at a very low frequency and further employed for the transfer of the K3 killer character into a commercial wine yeast. A novel Saccharomyces Bratislava 1K3 strain with desirable technological and anti-yeast ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814566
更新日期：1992-01-01 00:00:00
abstract：:Biochemical properties of a termostable alkaline phosphatase obtained from the mycelium extract of A. caespitosus were described. The enzyme was purified 42-fold with 32% recovery by DEAE-cellulose and concanavalin A-Sepharose chromatography. The molar mass estimated by Sephacryl S-200 or by 7% SDS-PAGE was 138 kDa an...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931303
更新日期：2007-01-01 00:00:00
abstract：:Two Bacillus cereus feather-degrading strains (23/1 and 6/2) were transformed using a recombinant plasmid p5.2 carrying the alkaline proteinase gene (aprE). A high level of the aprE gene expression was observed when the recombinant strains were grown on sporulation medium. The expression of the aprE gene proceeded dur...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-010-0004-y
更新日期：2010-01-01 00:00:00
abstract：:The aim of our study was to find out the optimal conditions for short-term storage of cerebrospinal fluid (CSF) samples for direct diagnosis of Lyme disease. A mixture of Borrelia-negative CSFs spiked with a defined amount of cultured Borrelia garinii was used. Borrelia stability was investigated over 7 days at four d...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-015-0408-9
更新日期：2016-01-01 00:00:00
abstract：:Virus-like particles (VLPs) were isolated from the yeast Wickerhamia fluorescens strain CCY61-1-1. The VLPs are approximately 42 nm in diameter and contain only one species of dsRNA molecule. The apparent length of the dsRNA determined by native agarose gel electrophoresis was 4.6 kbp. Analysis of protein content of t...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814620
更新日期：1996-01-01 00:00:00
abstract：:Based on the ability to attach to polymeric surfaces, the formation of biofilms was determined in 5 wild-type strains (Pseudomonas aeruginosa, Klebsiella pneumoniae, Acinetobacter baumanii, Escherichia coli, Staphylococcus warneri). Using modified Christensen method, minimum regrowth concentration (MRC) of piperacilli...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931650
更新日期：2004-01-01 00:00:00
abstract：:Stabilized sewage sludge is applied to agricultural fields and farmland due to its high organic matter content. The aim of this study was to investigate the effects of two types of sludge stabilization, mesophilic anaerobic digestion (MAD) and thermophilic anaerobic digestion (TAD), on bacterial communities in sludge,...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-015-0396-9
更新日期：2015-11-01 00:00:00
abstract：:Eleven purified lectins were compared in their IgE-precipitating, mast-cell-degranulating and histamine-releasing activity and in eliciting skin allergic reactions in atopic or normal people. A significantly positive relationship could be proved between their IgE-precipitating activity and degranulation of IgE-sensiti...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02923516
更新日期：1985-01-01 00:00:00
abstract：:A series of experiments were performed with P. aeruginosa to demonstrate which of the biochemical mechanisms are responsible for the resistance to the beta-lactam antibiotics. The constitutive beta-lactamase was isolated and characterized for the strain used as type OXA whose pI was 7.1, with a molar mass of 49 kg/mol...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02877216
更新日期：1987-01-01 00:00:00
abstract：:The sorption ability of Candida utilis biomass for cadmium ions with accumulating competence of dried cells and cells in alginate was compared. After an optimization of process conditions (pH 5.5, biomass concentration 1 g/L and c0 50 mg/L), the cadmium sorption capacity of dried yeast biomass was perceptibly higher t...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931807
更新日期：2006-01-01 00:00:00
abstract：:Manganese peroxidase (MnP) was produced by shallow stationary cultures of Phanerochaete chrysosporium growing on N-limited medium. Decolorization of sulfonphthalein (SP) dyes by MnP was investigated. The MnP activity profile and decolorization of SP dyes was correlated and almost all dyes were decolorized at pH 4.0. T...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931512
更新日期：2003-01-01 00:00:00
abstract：:Aliphatic 1,2-alkanolamines inhibited beta-glucanase from Candida utilis. The highest inhibitory effect was observed with 1-dodecylamino-3-chloro-2-propanol (Ki = 1.0 mumol/L) and it was much higher than that of D-glucono-delta-lactone. Simple 1,2-alkanolamines, like 2-aminoethanol or 2-methylaminoethanol, did not exh...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02898763
更新日期：1993-01-01 00:00:00
abstract：:Adhesion of group B streptococci to human epithelial vaginal and buccal cells proceeded in three phases which differed qualitatively. Maximum adhesion took place within 10 min of interaction, during the second phase (10-15 min), the percentage of adherent cells decreased significantly (P less than 0.05) whereas during...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02884088
更新日期：1983-01-01 00:00:00
abstract：:The mean lengths of G1, S, G2 and M phases of the cell cycle were determined on the basis of the population distribution of Candida utilis grown in a continuous culture under steady-state conditions by using an original mathematical method. The length of the G2 phase was proportional to that of G1; the length of M was...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02821281
更新日期：1990-01-01 00:00:00
abstract：:The color of light (white, red, blue, and green) had a significant effect on the growth and reproductive processes (both in the nucleocytoplasmic and chloroplast compartment of the cells) in synchronous cultures of Scenedesmus obliquus. This effect decreased in the order red > white > blue > green. In the same order, ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931828
更新日期：2006-01-01 00:00:00
abstract：:Common variable immunodeficiency (CVID) comprises a heterogeneous group of patients with as yet undefined genetic defects. Patients with CVID have in common a decrease in the levels of one or more serum immunoglobulin isotypes and a severe defect in the production of specific antibodies. Typically, the patients suffer...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/BF02814744
更新日期：1995-01-01 00:00:00
abstract：:The effect of dental plaque bacteria on LFA-1 beta expression on peripheral blood leukocytes was studied in 20 patients with early-onset periodontitis and in 10 healthy controls. Stimulation of PMN with selected dental plaque bacteria which play a role in the pathogeny of periodontitis significantly increased the expr...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02815697
更新日期：1996-01-01 00:00:00
abstract：:Staphylococcus epidermidis is a biofilm-forming bacterial strain that can cause major problems as an agent of nosocomial infections. Bacteria in biofilms are shielded from the environment and can survive high doses of antibiotics. We here test the antibiotic susceptibility of Staphylococcus epidermidis to rising genta...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-017-0568-x
更新日期：2018-05-01 00:00:00
