Abstract:
:Staphylococcus epidermidis is a biofilm-forming bacterial strain that can cause major problems as an agent of nosocomial infections. Bacteria in biofilms are shielded from the environment and can survive high doses of antibiotics. We here test the antibiotic susceptibility of Staphylococcus epidermidis to rising gentamicin concentrations in optimal growth conditions as used in routine bacteriology laboratories with low nutrient situations as suggested to be found in clinical situations. We found that gentamicin-resistant Staphylococcus epidermidis biofilms survived in the absence of external nutrient supply in PBS. While addition of gentamicin sulfate significantly reduced the pH value of all used media and solutions, this acidification did not alter survival of bacteria in the biofilm. We found a statistically significant and dose-dependent reduction of survival in low nutrient situations using gentamicin sulfate in three out of four patient isolates of Staphylococcus epidermidis which have been tested to be gentamicin-resistant under optimal growth conditions. Supporting the original profiling, survival in full media under the same antibiotic dosages was not significantly reduced. Our data here show that antibiotic resistance is a function of the provided nutrient concentration. Antibiotic resistance profiling should consider variations in nutrient availability.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Ammann CG,Neuhauser D,Eberl C,Nogler M,Coraça-Huber Ddoi
10.1007/s12223-017-0568-xsubject
Has Abstractpub_date
2018-05-01 00:00:00pages
299-305issue
3eissn
0015-5632issn
1874-9356pii
10.1007/s12223-017-0568-xjournal_volume
63pub_type
杂志文章abstract::The majority of antibiotics and substances with diverse biological activity used in medicine are produced by actinomycetes, nonfilamentous bacteria and fungi. Other microorganisms, such as myxobacteria, pseudomonads, nocardias, basidiomycetes, marine microorganisms, enterobacteria, halobacteria, hyperthermophiles etc....
journal_title:Folia microbiologica
pub_type: 杂志文章,评审
doi:10.1007/BF02993460
更新日期:2003-01-01 00:00:00
abstract::The effect of paraquat on the activities of antioxidant defense and detoxifying enzymes of the white-rot fungus Funalia trogii was determined. Paraquat increased the activities of glutathione reductase (GR), glutathione transferase (GT) and superoxide dismutase at 1 mmol/L, while at 0.1 mmol/L it did not affect the ac...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02818700
更新日期:2002-01-01 00:00:00
abstract::Mineral nutrition of crop plants is one of the major challenges faced by modern agriculture, particularly in arid and semi-arid regions. In alkaline calcareous soils, the availability of phosphorus and zinc is critically less due to their fixation and precipitation as complexes. Farmers use fertilizers to fulfill crop...
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abstract::Strains of Rhodospordium torloides produced two peptides into the culture filtrate. Peptide I contained 6 amino acids and peptide II contained 5 amino acids. The peptides however, are, not homologous to the alpha-factor, and become assimilated at a later phase of growth. ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02876614
更新日期:1980-01-01 00:00:00
abstract::Bifidobacteria (246 strains in total) were isolated from rectal samples of infants and adult humans and animals, and from intestinal samples of calves. Twenty-five strains grew well on mucin: 20 from infants, two from adults, and three from goatlings. Poor or no growth on mucin was observed in 156 bifidobacterial stra...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-011-0022-4
更新日期:2011-03-01 00:00:00
abstract::When studying the kinetics of DNA synthesis, growth and cell division in Escherichia coli B/r after irradiation with different doses of UV-radiation (254 nm) we could demonstrate, by means of pulse incorporation of 3H-thymidine, a lag in DNA synthesis after the irradiation. The relative rate of the restored DNA synthe...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02876446
更新日期:1982-01-01 00:00:00
abstract::Biofilms represent mixed communities present in a diverse range of environments; however, their utility as inoculants is less investigated. Our investigation was aimed towards in vitro development of biofilms using fungal mycelia (Trichoderma viride) as matrices and nitrogen-fixing and P-solubilizing bacteria as partn...
journal_title:Folia microbiologica
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doi:10.1007/s12223-012-0154-1
更新日期:2012-09-01 00:00:00
abstract::Secreted aspartyl proteinase (Sap) distribution among different C. albicans isolates was determined using SAP-specific primers in polymerase chain reaction (PCR) assay. SAP1, SAP2, and SAP3 were detected in 13 of 40 (32.5%), SAP4 in 38/40 (95%), SAP5 were detected in 30/40 (75%), SAP6 in 23/40 (57.5%) of C. albicans s...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931422
更新日期:2005-01-01 00:00:00
abstract::Cultivation media from 11 wood-rotting fungi contained alpha-mannosidase and mannanase activity, alpha-Mannosidase was studied in more detail in Phellinus abietis and mannanase was studied more intimately in basidiomycetes Phellinus abietis, Trametes sanguinea and Pholiota aurivella. Suitable cultivation conditions an...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02876996
更新日期:1977-01-01 00:00:00
abstract::In yeast, the STB5 gene encodes a transcriptional factor belonging to binuclear cluster class (Zn2Cys6) of transcriptional regulators specific to ascomycetes. In this study, we prepared the Kluyveromyces lactis stb5Δ strain and assessed its responses to different stresses. We showed that KlSTB5 gene is able to complem...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-019-00682-7
更新日期:2019-07-01 00:00:00
abstract::Three methods were used for the isolation of fungi in the present investigation: the dilution-plate method, hair-baiting technique with horse hairs and the "exposed plate" method. Sabouraud's glucose agar at 28 degrees C was also used as isolation medium. 102 species and 2 species varieties belonging to 36 genera were...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02821282
更新日期:1990-01-01 00:00:00
abstract::The temperature dependence of fluorescence anisotropy, lifetime and differential tangent of 1,6-diphenyl-1,3,5-hexatriene (DPH) and its polar trimethylammonium derivative (TMA-DPH) were investigated in cytoplasmic membranes of Bacillus subtilis. The fluorescence parameters were compared in the two types of membranes p...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02821406
更新日期:1990-01-01 00:00:00
abstract::The chloramphenicol resistance gene from pSa was introduced into T-DNA of pTi T37 of Agrobacterium tumefaciens by cointegration with intermediary plasmid based on pBR322. The resulting intermediary vector was mobilized to A. tumefaciens T37 by conjugative plasmid pRK2. The RK2 plasmid also forms contegrates with pTi d...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02926824
更新日期:1986-01-01 00:00:00
abstract::It is proposed that the transcription of the c-2 repressor gene initiates at two promoters, pre and prm, similarly to the transcription of the c-I gene of lambda phage. In the P22 phage, these promoters are defined by mutations of c54 and K5. ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02876780
更新日期:1975-01-01 00:00:00
abstract::Crude lipids isolated from Bacillus firmus, but not from other bacilli, were previously found to induce significant resistance against Listeria monocytogenes infection in mice. In this study, formaldehyde- and heat-killed bacterins of eight Bacillus species and some cellular fractions of B. firmus were prepared and te...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02906811
更新日期:1994-01-01 00:00:00
abstract::A total of 40 substances were tested for their inhibitory effect on the multiplication of a bacteriophage in a growing culture of Bacillus licheniformis and their influence on bacitracin production. Acriflavine was the only substance which, at a concentration of 3 microgram ml-1, completely suppressed phage multiplica...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02876597
更新日期:1978-01-01 00:00:00
abstract::Single chain Fv (scFv) antibodies (generated by phage display technology, molecules representing new and efficient tools in the research and diagnostics of infectious diseases) against the capsid protein (p25) of Maedi-Visna virus were selected. Several clones of p25 specific scFv antibodies were identified; one of th...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931380
更新日期:2003-01-01 00:00:00
abstract::The antifungal effect of 2-alkylthio-6-amino- and 2-alkylthio-6-formamidobenzothiazoles (22 derivatives in all) on Aspergillus niger and various Candida yeasts was tested. No significant effect was observed with A. niger. With the pathogenic yeast species C. albicans and C. guilliermondii, the most efficient derivativ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02814461
更新日期:1989-01-01 00:00:00
abstract::Sodium fluoride showed pH-dependent physiological responses in the two test microalgae Anabaena khannae and Chlorococcum humicola. A. khannae showed severe membrane damage with fluoride at low pH with leakage of pigments and electrolytes. Annihilation of photosynthesis along with inhibition in 14C uptake was observed ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931045
更新日期:2004-01-01 00:00:00
abstract::3-(1,1-Dioxadibenzothien-4-oyl)acrylic acid (1) was condensed with compounds containing active methylene groups under Michael reaction conditions to furnish the Michael adducts (lactones 2a-c, lactams 3a-c, ketones 4a,b). The behavior of these adducts toward the action of hydrazine hydrate were investigated. The compo...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02817670
更新日期:2002-01-01 00:00:00
abstract::Candida bracarensis is an emerging cryptic species within the Candida glabrata clade. To date, little is known about its epidemiology, virulence, and antifungal susceptibility. This study documents the occurrence of C. bracarensis for the first time in Mexico and focuses on its in vitro production of hydrolytic enzyme...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-018-0592-5
更新日期:2018-07-01 00:00:00
abstract::Kocuria spp. are widely distributed in nature. They are Gram-positive, coagulase-negative, coccoid bacteria belonging to the family Micrococcaceae, suborder Micrococcineae, order Actinomycetales, class Actinobacteria. In general, limited knowledge exists concerning the properties associated with the representants of t...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-015-0431-x
更新日期:2016-05-01 00:00:00
abstract::Common variable immunodeficiency (CVID) comprises a heterogeneous group of patients with as yet undefined genetic defects. Patients with CVID have in common a decrease in the levels of one or more serum immunoglobulin isotypes and a severe defect in the production of specific antibodies. Typically, the patients suffer...
journal_title:Folia microbiologica
pub_type: 杂志文章,评审
doi:10.1007/BF02814744
更新日期:1995-01-01 00:00:00
abstract::Blastoschizomyces capitatus is an uncommon, opportunistic pathogenic fungus, which causes invasive and disseminated infections. This microorganism is normally present in both environmental and normal human flora. Within a host, B. capitatus is able to grow in both unicellular yeast and multicellular filamentous growth...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-013-0273-3
更新日期:2014-03-01 00:00:00
abstract::Virulence factors were compared in 15 Aeromonas spp. isolated from faeces of patients with Aeromonas-associated gastroenteritis and in 81 strains isolated from food. Strains from food did not show differences in the distribution of virulence factors when compared with strains isolated from faeces. However, 88.8% of Ae...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02814323
更新日期:1994-01-01 00:00:00
abstract::One-week dextran sulfate treatment of conventional (CV) immunodeficient (SCID) mice gave rise to acute colitis in the colon mucosa; germ-free (GF) SCID mice did not exhibit any changes in colon morphology. Dextran sulfate application to CV immunocompetent (BALB/c) mice did induce substantial changes in the colon mucos...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02818004
更新日期:2001-01-01 00:00:00
abstract::Treatment of human group C and G streptococci with cyanogen bromide results in solubilization of surface protein G molecules. Strain-to-strain variation in the quantity, molar mass and functional activity of protein G extracted from representative group C and G isolates led to the identification of three structurally ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02825666
更新日期:1999-01-01 00:00:00
abstract::The postantibiotic effect (PAE) and the postantibiotic sub-MIC effect (PASME) of ciprofloxacin, pefloxacin and amikacin were studied for Salmonella typhimurium and S. enteritidis strains. PAE was induced by 2 x and 4 x MIC of antibiotics studied for 0.5 h. After PAE and PASME their effect on prophage induction of a ly...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02816944
更新日期:1997-01-01 00:00:00
abstract::A gram-negative bacterium GXGL-4A was originally isolated from maize roots. It displayed nitrogen-fixing (NF) ability under nitrogen-free culture condition, and had a significant promotion effect on cucumber growth in the pot inoculation test. The preliminary physiological and biochemical traits of GXGL-4A were charac...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-018-0608-1
更新日期:2018-11-01 00:00:00
abstract::Enzyme synthesis of tryptophan from indole, pyruvate and ammonium salts was studied using Escherichia coli cells exhibiting a significant tryptophanase activity. In addition to the effect of cultivation medium composition and cultivation conditions, factors affecting the course of the conversion were investigated. Pro...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02820485
更新日期:1990-01-01 00:00:00