Abstract：
:Biofilms represent mixed communities present in a diverse range of environments; however, their utility as inoculants is less investigated. Our investigation was aimed towards in vitro development of biofilms using fungal mycelia (Trichoderma viride) as matrices and nitrogen-fixing and P-solubilizing bacteria as partners, as a prelude to their use as biofertilizers (biofilmed biofertilizers, BBs) and biocontrol agents for different crops. The most suitable media in terms of population counts, fresh mass and dry biomass for Trichoderma and Bacillus subtilis/Pseudomonas fluorescens was found to be Pikovskaya broth ± 1 % CaCO(3), while for Trichoderma and Azotobacter chroococcum, Jensen's medium was most optimal. The respective media were then used for optimization of the inoculation rate of the partners in terms of sequence of addition of partners, fresh/dry mass of biofilms and population counts of partners for efficient film formation. Microscopic observations revealed significant differences in the progress of growth of biofilms and dual cultures. In the biofilms, the bacteria were observed growing intermingled within the fungal mycelia mat. Further, biofilm formation was compared under static and shaking conditions and the fresh mass of biofilms was higher in the former. Such biofilms are being further characterized under in vitro conditions, before using them as inoculants with crops.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Triveni S,Prasanna R,Saxena AKdoi
10.1007/s12223-012-0154-1subject
Has Abstractpub_date
2012-09-01 00:00:00pages
431-7issue
5eissn
0015-5632issn
1874-9356journal_volume
57pub_type
杂志文章abstract：:Fibrobacter succinogenes is a major cellulolytic species in the rumen. On the basis of molecular data, this species can be classified into four phylogenetic groups. Recently gathered ecological and physiological data have revealed the importance of this species, particularly phylogenetic group 1, in rumen fiber digest...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/s12223-008-0024-z
更新日期：2008-01-01 00:00:00
abstract：:Patients suffering from periodontitis or periodontosis were selected for the study. Further subdivision of these groups was based on the presence or absence of herpes and/or adenoviruses in their oral lymphocytes and epithelial cells. The phagocytic and bactericidal activities of oral leukocytes isolated from the same...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02887577
更新日期：1987-01-01 00:00:00
abstract：:Among 18 tested strains of Claviceps spp., 7 produced significant amounts of exocellular polysaccharide (EPS). The maximum production of EPS was found in fermentation broth of Claviceps viridis. The kinetics of growth, substrate consumption, and EPS production in the batch, aerobic, submerged culture of this fungus we...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02993470
更新日期：2003-01-01 00:00:00
abstract：:We present the results of analysis of membrane phosphoproteomes from individual morphological stages of Streptomyces coelicolor that reflect developmentally dependent heterogeneity and phosphorylation of intrinsic and externally added purified Strepomyces aureofaciens EF-Tu. Fast growing nonpathogenic Mycobacterium sm...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-010-0029-2
更新日期：2010-05-01 00:00:00
abstract：:Murine herpesvirus 68 (MHV-68) is a natural pathogen that infects murid rodents, which serves as hosts for Ixodes ricinus ticks. For the first time, MHV-68 was detected in immature I. ricinus ticks feeding on Lacerta viridis lizards trapped in Slovakia, which supports the idea that ticks can acquire the virus from fee...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-018-0586-3
更新日期：2018-07-01 00:00:00
abstract：:Strains of Rhodospordium torloides produced two peptides into the culture filtrate. Peptide I contained 6 amino acids and peptide II contained 5 amino acids. The peptides however, are, not homologous to the alpha-factor, and become assimilated at a later phase of growth. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02876614
更新日期：1980-01-01 00:00:00
abstract：:The submerged culture of the Claviceps purpurea strain studied was polymorphous. In the process of alkaloid synthesis, cytodifferentiation preceded biochemical differentiation. The onset of the alkaloid phase was characterized by: predominance of chlamydospores in the culture, the presence of vegetative cells with red...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02876766
更新日期：1975-01-01 00:00:00
abstract：:Ultrastructural changes induced by Rickettsia slovaca standard type (ST) and wild type (WT) were examined during their life cycle in L929 and Vero cells. R. slovaca invaded the cytoplasm of the host cell by phagocytosis on the 1st d p.i. Rickettsiae adhering to the cytoplasmic membrane were engulfed by cellular extens...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-009-0019-4
更新日期：2009-01-01 00:00:00
abstract：:Lactobacillus pobuzihii is a novel species which has been previously found in pobuzihi (fermented cummingcordia), a traditional fermented food in Taiwan. However, the lactic acid bacteria (LAB) microflora in pobuzihi has not been studied in detail. In this study, LAB from pobuzihi were isolated, identified, and charac...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-012-0188-4
更新日期：2013-03-01 00:00:00
abstract：:Viridans group streptococci bloodstream infections (VGS BSI) remain a significant cause of mortality and morbidity in patients with severe neutropenia. The goal of our study was to evaluate clinical course and microbiological susceptibility of VGS BSI at our center. Retrospective analysis of all microbiologically docu...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-017-0542-7
更新日期：2018-03-01 00:00:00
abstract：:Small plasmid pKST23 was isolated from sheep ruminal Escherichia coli population. Plasmid sequence was determined to be 2,779 bp in length and was found to have an overall 42 % of GC pairs. However, its sequence can be divided into two regions based on genetic composition and the GC content. It was found that the high...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-012-0124-7
更新日期：2012-07-01 00:00:00
abstract：:The production of bacterial enzymes in batch fermentations is compared with results obtained in continuous culture. When studying the production of alpha-amylase in Bacillus subtilis it was found that instability of the enzyme synthesis was due to nonhomogeneity of the population rather than to "the culture's history"...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02883137
更新日期：1982-01-01 00:00:00
abstract：:A quarantine organism, "Candidatus Phytoplasma mali," is the causal agent of apple proliferation, one of the most important apple diseases in Europe. The genetic diversity of this pathogen in Central and Southern Europe has already been reported; however, almost no data exists from Eastern Europe. In this study, "Ca. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-018-0660-x
更新日期：2019-05-01 00:00:00
abstract：:Phosphatidylcholine is one of the most frequent phospholipid components of the inner mitochondrial membrane of Neurospora crassa. Quantitative analysis of phosphlipids of the wild strain of Neurospora crassa and of its two cho mutants showed that these strains did not significantly differ in the content of phosphatidy...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02879763
更新日期：1982-01-01 00:00:00
abstract：:Mitochondria isolated from Neurospora crassa were purified by centrifugation in a Percoll density gradient. Enzyme activities and cytochrome differential spectra revealed a high purity of the mitochondria. As compared with a crude mitochondrial fraction the purified mitochondria exhibited a high respiratory activity a...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02879491
更新日期：1983-01-01 00:00:00
abstract：:The sorption ability of Candida utilis biomass for cadmium ions with accumulating competence of dried cells and cells in alginate was compared. After an optimization of process conditions (pH 5.5, biomass concentration 1 g/L and c0 50 mg/L), the cadmium sorption capacity of dried yeast biomass was perceptibly higher t...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931807
更新日期：2006-01-01 00:00:00
abstract：:Skin microbiome main cultivable aerobes in human are coagulase-negative staphylococci and lipophilic corynebacteria. Staphylococcus strains (155) belonging to 10 species and 105 strains of Corynebacterium belonging to nine species from the skin swabs of healthy male volunteers were investigated to determine their enzy...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-014-0326-2
更新日期：2014-11-01 00:00:00
abstract：:The effect of dental plaque bacteria on LFA-1 beta expression on peripheral blood leukocytes was studied in 20 patients with early-onset periodontitis and in 10 healthy controls. Stimulation of PMN with selected dental plaque bacteria which play a role in the pathogeny of periodontitis significantly increased the expr...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02815697
更新日期：1996-01-01 00:00:00
abstract：:Purification of streptococcal extracellular NAD+ nucleosidase is associated with changes of kinetic properties. A high-molecular weight component is required for a full activity of the enzyme. The component is not produced by bacteria and is present in the Todd-Hewitt cultivation medium, the beef-heart extract serving...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02876396
更新日期：1980-01-01 00:00:00
abstract：:Propionate at concentrations up to 0.05% decreased at 25 degrees C the growth and sporulation of Penicillium aurantiogriseum. The standard size of conidiogenous structures (metulae, phialids) and conidia was diminished. The effect was more pronounced at a higher temperature (30 degrees C). Inhibition of ochratoxin pro...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814202
更新日期：1995-01-01 00:00:00
abstract：:Overpopulation of domestic pigeons is considered to be one of the major problems of urban centers, as these birds are responsible for the dissemination of relevant pathogens to animal and human health. The aim of this study was to detect potentially pathogenic Escherichia coli and Salmonella spp. in domestic pigeons c...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-020-00781-w
更新日期：2020-08-01 00:00:00
abstract：:Based on the ability to attach to polymeric surfaces, the formation of biofilms was determined in 5 wild-type strains (Pseudomonas aeruginosa, Klebsiella pneumoniae, Acinetobacter baumanii, Escherichia coli, Staphylococcus warneri). Using modified Christensen method, minimum regrowth concentration (MRC) of piperacilli...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931650
更新日期：2004-01-01 00:00:00
abstract：:Physiological and biochemical properties were tested in 45 isolates of Aspergillus egyptiacus (16 isolates), Emericella nidulans (16) and Aspergillus versicolor (13). The three fungal species exhibited common and similar features. The big similarity between A. egyptiacus and E. nidulans was greater than between A. egy...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814449
更新日期：1994-01-01 00:00:00
abstract：:Streptomyces aureofaciens glucosidizes 1,2,4-trihydroxy-9,10-anthraquinone (purpurin) added to the cultivation medium to yield the corresponding 2-beta-D-glucoside. The identity of the glucoside was demonstrated by comparing its physico-chemical properties with data of an authentic sample prepared synthetically. A fur...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02873156
更新日期：1984-01-01 00:00:00
abstract：:Streptomyces caelestis DSM 40084 produces two osmolytes, viz. 2-O-(alpha-D-glucopyranosyl)-zeta-glyceric acid (GG) and trehalose. Both compounds were isolated and identified by nuclear magnetic resonance spectroscopy and mass spectrometry. A very sensitive regulation of the cell osmolytes was demonstrated in exponenti...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02932103
更新日期：2007-01-01 00:00:00
abstract：:Resistance determinants for HgCl2 and CdCl2 were eliminated along with a number of antibiotic resistance factors from Acinetobacter lwoffi and Micrococcus sp. at 44 degrees C. These organisms were orginally resistant to HgCl2, merbromin, CdCl2, Pb(NO3)2, benzylpenicillin, erythromycin, carbenicillin, tetracycline and ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02925907
更新日期：1988-01-01 00:00:00
abstract：:A comparative examination of reduced methyl [MV·](+) and benzyl [BV·](+) viologens (as artificial electron donors for quantitative estimation of the respiratory periplasmic (Nap) and membrane-embedded (Nar) nitrate reductases) using a newly constructed nap mutant strain of Paracocccus denitrificans was done. The activ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-011-0004-6
更新日期：2011-01-01 00:00:00
abstract：:The majority of antibiotics and substances with diverse biological activity used in medicine are produced by actinomycetes, nonfilamentous bacteria and fungi. Other microorganisms, such as myxobacteria, pseudomonads, nocardias, basidiomycetes, marine microorganisms, enterobacteria, halobacteria, hyperthermophiles etc....
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/BF02993460
更新日期：2003-01-01 00:00:00
abstract：:The microflora of the chernozem soil mineralized 62% of the lactose retained on a column consisting of three 10-g layers of the soil at a daily flow of 48 mg of the sugar. Only 45% of the sugar was mineralized when the daily flow was 136 mg. The highest value of the beta-galactosidase activity in the system of heteroc...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02927259
更新日期：1981-01-01 00:00:00
abstract：:One hundred clinical isolates of Pseudomonas aeruginosa were checked for their sensitivity towards silver nitrate. Majority of the isolates were resistant at 20 mg/L and the resistance decreased with increasing concentration of silver nitrate, only 5% of the organisms showed resistance above 70 mg/L. These silver-resi...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02820752
更新日期：1989-01-01 00:00:00
