Nitrate reductase whole-cell assay: side effects associated with the use of benzyl viologen.

Abstract:

:A comparative examination of reduced methyl [MV·](+) and benzyl [BV·](+) viologens (as artificial electron donors for quantitative estimation of the respiratory periplasmic (Nap) and membrane-embedded (Nar) nitrate reductases) using a newly constructed nap mutant strain of Paracocccus denitrificans was done. The activity with [MV·](+) was high in whole-cell assays, confirming that this compound donates electrons to Nar. Initial rates of the more lipophilic [BV·](+) were considerably lower, which was interpreted to be caused by an inhibition of the active transport of nitrate into the cells. Anionophoric activity of [BV·](+) was detectable but too low to effectively circumvent the inhibition of nitrate transporter.

journal_title

Folia microbiologica

authors

Illichmanová A,Janů M,Sedláček V,Kučera I

doi

10.1007/s12223-011-0004-6

subject

Has Abstract

pub_date

2011-01-01 00:00:00

pages

72-6

issue

1

eissn

0015-5632

issn

1874-9356

journal_volume

56

pub_type

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