Abstract:
:In this study, a method combining Raman spectroscopy with chemometric analysis was developed for detection of phage presence in raw milk and discrimination of Streptococcus thermophilus and Lactobacillus bulgaricus phages which are among the main phages causing problems in dairy industry. For this purpose, S. thermophilus and L. bulgaricus phages were added into raw milk separately, and then some pretreatments such as fat separation, removal of casein, and filtration were applied to the raw milk samples. Raman spectra of the samples were collected and then analyzed using principal component analysis in order to discriminate these phages in raw milk. In the next step, dilutions of S. thermophilus phages in pretreated raw milk were prepared, and Raman spectra were collected. These spectra were analyzed by using partial least squares method to quantify phages in low titer. Consequently, it has been demonstrated that S. thermophilus and L. bulgaricus phages, which have titers sufficient to fail the fermentation (~ 107 pfu/mL) and have lower titers (102-103 pfu/mL), could be discriminated from antibiotic and each other. Additionally, low concentrations of S. thermophilus phages (102 pfu/mL) could be detected through Raman spectroscopy with a short analysis time (60 min) and high coefficient of determination (R2) values for both calibration (0.985) and validation (0.906) with a root mean square error of calibration of 70.54 and root mean square error of prediction of 165.47. However, a lower success was achieved with L. bulgaricus phages and the obtained coefficient of determination values were not sufficiently high (0.649).
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Tayyarcan EK,Acar Soykut E,Boyaci IHdoi
10.1007/s12223-018-0604-5subject
Has Abstractpub_date
2018-09-01 00:00:00pages
627-636issue
5eissn
0015-5632issn
1874-9356pii
10.1007/s12223-018-0604-5journal_volume
63pub_type
杂志文章abstract::Differences in the effect of volatile and gaseous metabolites of germinating pea seeds on the germination of spores of Mucor racemosus and macroconidia of Fusarium oxysporum are described. Germination of spores of M. racemosus was inhibited by seed metabolites whereas germination of macroconidia of F. oxysporum was st...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02933019
更新日期:1980-01-01 00:00:00
abstract::The gene encoding the coat protein (CP) of a potato virus Y (PVY) was cloned into expression vector pMPM-A4Omega. PVY CP was expressed in Escherichia coli and the purified recombinant protein was used for raising rabbit polyclonal antibodies. The sera and antibodies were tested for the detection of PVY in the laborato...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-008-0067-1
更新日期:2008-01-01 00:00:00
abstract::Based on the ability to attach to polymeric surfaces, the formation of biofilms was determined in 5 wild-type strains (Pseudomonas aeruginosa, Klebsiella pneumoniae, Acinetobacter baumanii, Escherichia coli, Staphylococcus warneri). Using modified Christensen method, minimum regrowth concentration (MRC) of piperacilli...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931650
更新日期:2004-01-01 00:00:00
abstract::The levels of anti-Candida antibodies were determined in experimental animals immunized with 4 different yeast doses (0.3, 0.6, 1.2, 2.4 x 10(8) CFU) at weekly intervals. After immunization (sampling intervals 1, 2, and 3 months), the intravenous blood was examined for the presence of serum anti-Candida antibodies. Af...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931340
更新日期:2003-01-01 00:00:00
abstract::Addition of glucose to a resting cell suspension of the yeast Saccharomyces cerevisiae was accompanied by marked shifts of the G alpha-protein subunits from the plasma membrane to the cell interior. This process was rapid with half-times between < 10 and 20 s. The decrease of the plasma membrane pool of the Gi alpha/G...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02814427
更新日期:2001-01-01 00:00:00
abstract::Strains ofRhodotorula sp.,Candida spp. andLangermania sp. cultivated on polyunsaturated oil preferentially incorporated more unsaturated fatty acids. These fatty acids were used mainly for growth needs whereas the saturated ones accumulated in the microbial cell. The cellular oil and the remaining oil in the culture h...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02898718
更新日期:1997-04-01 00:00:00
abstract::Moderately halophilic bacteria utilizing phenol as the sole carbon source were isolated by selective enrichment from sea water. The isolate (Gram-negative motile rods) was identified as Deleya venusta. It grew well in the presence of up to 1600 mg/L of phenol and 8% NaCl under aerobic conditions. When the cells were t...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02815617
更新日期:2001-01-01 00:00:00
abstract::Among 18 tested strains of Claviceps spp., 7 produced significant amounts of exocellular polysaccharide (EPS). The maximum production of EPS was found in fermentation broth of Claviceps viridis. The kinetics of growth, substrate consumption, and EPS production in the batch, aerobic, submerged culture of this fungus we...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02993470
更新日期:2003-01-01 00:00:00
abstract::Virulence factors were compared in 15 Aeromonas spp. isolated from faeces of patients with Aeromonas-associated gastroenteritis and in 81 strains isolated from food. Strains from food did not show differences in the distribution of virulence factors when compared with strains isolated from faeces. However, 88.8% of Ae...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02814323
更新日期:1994-01-01 00:00:00
abstract::Inactivation of seven strains derived from Escherichia coli B differing in their capacity to repair damage to their DNA (exc, pol, rec) after irradiation with far (254 nm) and middle and near (300 to 380 and 320--400 nm) UV light was investigated. The same bacterial strains were also used as hosts for the UV-irradiate...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02876690
更新日期:1980-01-01 00:00:00
abstract::Ultrastructural changes induced by Rickettsia slovaca standard type (ST) and wild type (WT) were examined during their life cycle in L929 and Vero cells. R. slovaca invaded the cytoplasm of the host cell by phagocytosis on the 1st d p.i. Rickettsiae adhering to the cytoplasmic membrane were engulfed by cellular extens...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-009-0019-4
更新日期:2009-01-01 00:00:00
abstract::Small plasmid pKST23 was isolated from sheep ruminal Escherichia coli population. Plasmid sequence was determined to be 2,779 bp in length and was found to have an overall 42 % of GC pairs. However, its sequence can be divided into two regions based on genetic composition and the GC content. It was found that the high...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-012-0124-7
更新日期:2012-07-01 00:00:00
abstract::In yeast, the STB5 gene encodes a transcriptional factor belonging to binuclear cluster class (Zn2Cys6) of transcriptional regulators specific to ascomycetes. In this study, we prepared the Kluyveromyces lactis stb5Δ strain and assessed its responses to different stresses. We showed that KlSTB5 gene is able to complem...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-019-00682-7
更新日期:2019-07-01 00:00:00
abstract::During cultivation in a minimal medium with glucose Alternaria tenuissima and Aspergillus vesicolor produce constitutively alpha- and beta-glucanases. Fractions of beta-1,3-glucanases exhibiting affinity for laminarin were separated by means of gel filtration chromatography. Two neutral beta-1,3-glucanases with affini...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02876394
更新日期:1980-01-01 00:00:00
abstract::Hemolytic factor production by pathogenic Candida species is considered an important attribute in promoting survival within the mammal host through the ability to assimilate iron from the hemoglobin-heme group. Hemolytic capability has been evaluated for Candida species based on hemolysis zones on plate assay, analysi...
journal_title:Folia microbiologica
pub_type: 杂志文章,评审
doi:10.1007/s12223-018-0584-5
更新日期:2018-07-01 00:00:00
abstract::Rabbits, sensitized with M. kansasii, responded by a profound inhibition of migration of macrophages elicited by both antigens: the migration index for homologous antigen was 0.51 in the direct test and 0.65 in the indirect test; for heterologous antigen the indexes were 0.53 and 0.67. However, significant differences...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02877345
更新日期:1980-01-01 00:00:00
abstract::Crude lipids isolated from Bacillus firmus, but not from other bacilli, were previously found to induce significant resistance against Listeria monocytogenes infection in mice. In this study, formaldehyde- and heat-killed bacterins of eight Bacillus species and some cellular fractions of B. firmus were prepared and te...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02906811
更新日期:1994-01-01 00:00:00
abstract::Treatment of human group C and G streptococci with cyanogen bromide results in solubilization of surface protein G molecules. Strain-to-strain variation in the quantity, molar mass and functional activity of protein G extracted from representative group C and G isolates led to the identification of three structurally ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02825666
更新日期:1999-01-01 00:00:00
abstract::Regulatory mechanism of primary metabolism responsible for the biosynthesis of peptide ergot alkaloids was proposed on the basis of experimental results from the production phase of Claviceps purpurea (FR.) Tul. in parasitic and saprophytic cultures. The production-phase metabolism is characterized by uncoupling of gl...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02927365
更新日期:1981-01-01 00:00:00
abstract::Fungal cells of Aureobasidium pullulans ATCC 201253 were immobilized by entrapment in chitosan beads, and the immobilized cells were investigated for their ability to produce the polysaccharide pullulan using batch fermentation. The 1% chitosan-entrapped fungal cells were capable of producing pullulan for two cycles o...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-011-0048-7
更新日期:2011-07-01 00:00:00
abstract::We report a novel application of calcein-acetomethyl ester in flow cytometry for rapid estimation of the number of G+-bacteria in rodent feces (Apodemus sylvaticus and Mus sp.f. muridae). We also use the combined application of flow cytometry and Syto-13 or Sypro Orange staining to count rapidly the total bacterial po...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931538
更新日期:2004-01-01 00:00:00
abstract::A total of 152 lactic acid bacteria (LAB) were isolated from pickles produced in the Ankara-Çubuk region. These isolates were clustered into eight groups on the basis of their phenotypic characteristics including cell morphology, CO2 production from glucose, growth at 10 and 45 °C, growth in 6.5 % NaCl, and growth at ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-014-0363-x
更新日期:2015-05-01 00:00:00
abstract::Purification of streptococcal extracellular NAD+ nucleosidase is associated with changes of kinetic properties. A high-molecular weight component is required for a full activity of the enzyme. The component is not produced by bacteria and is present in the Todd-Hewitt cultivation medium, the beef-heart extract serving...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02876396
更新日期:1980-01-01 00:00:00
abstract::The newly described type strains Arcanobacterium pinnipediorum DSM 28752T and Arcanobacterium wilhelmae DSM 102162T, initially isolated from an anal swab of a harbor seal (Sammra et al. Int J Syst Evol Microbiol 65:4539-4543, 2015) and the genital tract of a rhinoceros (Sammra et al. Int J Syst Evol Microbiol 67:2093-...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-018-0610-7
更新日期:2018-11-01 00:00:00
abstract::The uvrE (E. coli KS 114) cells carry a mutation in the gene that codes for helicase II. This is the protein responsible for replicative unwinding of double-helical DNA. The repair mode of such cells may be altered as compared with the wild type. The survival of uvrE cells during postirradiation incubation under inhib...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02922491
更新日期:1985-01-01 00:00:00
abstract::A wild-type strain of Candida albicans (S1, ATCC 10261) was used to obtain stable auxotrophic colony morphological mutants (mutant M5 producing only true hyphae and mutant M2 containing 90 % blastospores and 10 % pseudohyphae) by induced mutagenesis. A hybrid was produced by somatic hybridization between these 2 mutan...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931528
更新日期:2004-01-01 00:00:00
abstract::Stabilized sewage sludge is applied to agricultural fields and farmland due to its high organic matter content. The aim of this study was to investigate the effects of two types of sludge stabilization, mesophilic anaerobic digestion (MAD) and thermophilic anaerobic digestion (TAD), on bacterial communities in sludge,...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-015-0396-9
更新日期:2015-11-01 00:00:00
abstract::Aliphatic 1,2-alkanolamines inhibited beta-glucanase from Candida utilis. The highest inhibitory effect was observed with 1-dodecylamino-3-chloro-2-propanol (Ki = 1.0 mumol/L) and it was much higher than that of D-glucono-delta-lactone. Simple 1,2-alkanolamines, like 2-aminoethanol or 2-methylaminoethanol, did not exh...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02898763
更新日期:1993-01-01 00:00:00
abstract::A selection of tert-butylhydroperoxide (tBOOH)-tolerant Candida albicans mutants showed increased tolerances to 19 different stress conditions. These mutants are characterized by a constitutively upregulated antioxidative defense system and, therefore, adaptation to oxidative stress may play an important role in gaini...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-014-0305-7
更新日期:2014-07-01 00:00:00
abstract::It is proposed that the transcription of the c-2 repressor gene initiates at two promoters, pre and prm, similarly to the transcription of the c-I gene of lambda phage. In the P22 phage, these promoters are defined by mutations of c54 and K5. ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02876780
更新日期:1975-01-01 00:00:00