Abstract:
:The uvrE (E. coli KS 114) cells carry a mutation in the gene that codes for helicase II. This is the protein responsible for replicative unwinding of double-helical DNA. The repair mode of such cells may be altered as compared with the wild type. The survival of uvrE cells during postirradiation incubation under inhibition of de novo protein synthesis was increased which indicates that this process of repair in uvrE cells is mediated by constitutive proteins and does not require any inducible products but takes a certain time. This inhibition of de novo protein synthesis causes also an inhibition of dimer excision, an increase of the parental DNA degradation and a decrease of parental and daughter DNA molar mass. On the other hand, it seems that induced proteins are formed in uvrE cells after UV irradiation but their influence is low in inducible repair and they can act only under conditions of complete protein synthesis.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Bencová Mdoi
10.1007/BF02922491subject
Has Abstractpub_date
1985-01-01 00:00:00pages
3-16issue
1eissn
0015-5632issn
1874-9356journal_volume
30pub_type
杂志文章abstract::Two Bacillus cereus feather-degrading strains (23/1 and 6/2) were transformed using a recombinant plasmid p5.2 carrying the alkaline proteinase gene (aprE). A high level of the aprE gene expression was observed when the recombinant strains were grown on sporulation medium. The expression of the aprE gene proceeded dur...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-010-0004-y
更新日期:2010-01-01 00:00:00
abstract::This is the first report describing precipitation of bacteriophage particles with zinc chloride as a method of choice to isolate infectious lytic bacteriophages against Pectobacterium spp. and Dickeya spp. from environmental samples. The isolated bacteriophages are ready to use to study various (ecological) aspects of...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-015-0411-1
更新日期:2016-01-01 00:00:00
abstract::The induction of tryptophanase was not affected by rifampicin in the rifampicin-resistant mutant of Vibrio el tor while the antibiotic inhibited the induction of tryptophanase in the normal strain at level of ribonucleic acid and protein synthesis. ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02877677
更新日期:1977-01-01 00:00:00
abstract::Aliphatic 1,2-alkanolamines inhibited beta-glucanase from Candida utilis. The highest inhibitory effect was observed with 1-dodecylamino-3-chloro-2-propanol (Ki = 1.0 mumol/L) and it was much higher than that of D-glucono-delta-lactone. Simple 1,2-alkanolamines, like 2-aminoethanol or 2-methylaminoethanol, did not exh...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02898763
更新日期:1993-01-01 00:00:00
abstract::Hemolytic factor production by pathogenic Candida species is considered an important attribute in promoting survival within the mammal host through the ability to assimilate iron from the hemoglobin-heme group. Hemolytic capability has been evaluated for Candida species based on hemolysis zones on plate assay, analysi...
journal_title:Folia microbiologica
pub_type: 杂志文章,评审
doi:10.1007/s12223-018-0584-5
更新日期:2018-07-01 00:00:00
abstract::Cardiolipin synthesis was studied during the aerobic synchronous growth of aerobically grown yeast Saccharomyces cerevisiae. The time course of the synthesis was stepwise and the rise in cardiolipin level in cells coincided in time with the increase in cytochrome oxidase activity. This finding supports the notion of d...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02876990
更新日期:1977-01-01 00:00:00
abstract::Contemporary aspects of cerebrospinal fluid analysis are discussed, including the relationship to neuro-infective, autoimmune and other neurological diseases. The actual state of cerebrospinal fluid microbiological and cytological investigation and analysis of cerebrospinal fluid protein fractions are described in det...
journal_title:Folia microbiologica
pub_type: 杂志文章,评审
doi:10.1007/BF02931522
更新日期:2003-01-01 00:00:00
abstract::The aim of this study was to evaluate the contribution of plasmid-mediated genes and efflux to fluoroquinolone resistance in collection of Achromobacter spp. gathered during a 3-year period. Susceptibility to ciprofloxacin and levofloxacin was tested by disk diffusion and microdilution tests for a collection of 98 Ach...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-018-0639-7
更新日期:2019-03-01 00:00:00
abstract::Small plasmid pKST23 was isolated from sheep ruminal Escherichia coli population. Plasmid sequence was determined to be 2,779 bp in length and was found to have an overall 42 % of GC pairs. However, its sequence can be divided into two regions based on genetic composition and the GC content. It was found that the high...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-012-0124-7
更新日期:2012-07-01 00:00:00
abstract::Stabilized sewage sludge is applied to agricultural fields and farmland due to its high organic matter content. The aim of this study was to investigate the effects of two types of sludge stabilization, mesophilic anaerobic digestion (MAD) and thermophilic anaerobic digestion (TAD), on bacterial communities in sludge,...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-015-0396-9
更新日期:2015-11-01 00:00:00
abstract::Addition of glucose to a resting cell suspension of the yeast Saccharomyces cerevisiae was accompanied by marked shifts of the G alpha-protein subunits from the plasma membrane to the cell interior. This process was rapid with half-times between < 10 and 20 s. The decrease of the plasma membrane pool of the Gi alpha/G...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02814427
更新日期:2001-01-01 00:00:00
abstract::Cryptococcus neoformans is encapsulated yeast that causes cryptococcosis. The cryptococcal meningitis may cause neuropsychiatric symptoms. Here, we evaluated the in vitro activity of amphotericin B (AMB), chlorpromazine (CLOR), and sertraline (SERT) alone or in combination against clinical isolates of C. neoformans co...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-016-0449-8
更新日期:2016-09-01 00:00:00
abstract::The chloramphenicol resistance gene from pSa was introduced into T-DNA of pTi T37 of Agrobacterium tumefaciens by cointegration with intermediary plasmid based on pBR322. The resulting intermediary vector was mobilized to A. tumefaciens T37 by conjugative plasmid pRK2. The RK2 plasmid also forms contegrates with pTi d...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02926824
更新日期:1986-01-01 00:00:00
abstract::Vinasse, a recalcitrant waste of the ethanol industry was employed for the production of polyhydroxyalkanoate (PHA) by the extremely halophilic archaeon, Haloarcula marismortui in shake flasks. The PHA was recovered by osmotic lysis of the cells and subsequent purification by sodium hypochlorite and organic solvents. ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-011-0092-3
更新日期:2012-01-01 00:00:00
abstract::A hexokinase preparation was obtained from a Saccharomyces cerevisiae mutant strain deficient in glucosephosphate isomerase (GPI) and mannosephosphate isomerase (MPI) by precipitation with ammonium sulfate. The supernatant fraction corresponding to 40-60 % saturation showed the lowest content in GPI and MPI activity. ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02927364
更新日期:1981-01-01 00:00:00
abstract::An experimental model in white mice, infected with a mildly virulent strain of Klebsiella pneumoniae, was elaborated for studies on local immunity of the respiratory tract. Instillation of klebsiella into the supralaryngeal space of anaesthetised animals proved to be more suitable than the commonly used method of intr...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02879494
更新日期:1983-01-01 00:00:00
abstract::Raoultella spp. representatives are Gram-negative capsulated, nonmotile rods. These bacteria are found in the natural environment: plants, water, soil and insects. R. ornithinolytica is one of the three species of Raoultella. R. ornithinolytica is the only species within the genus which has the ability to produce orni...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-015-0390-2
更新日期:2015-11-01 00:00:00
abstract::Strains of genus Citrobacter (152 isolates from 1950 to 1988 deposited in the Czech National Collection of Type Cultures, Prague) were re-classified using biological and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) methods. One-hundred thirty-six strains (ca. 90 %) were i...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-014-0340-4
更新日期:2015-01-01 00:00:00
abstract::Frequency of heterocytes and nitrogenase activity (NA) under light and dark cultivation conditions was determined in 12 cyanobacterial strains isolated from various soil habitats. In spite of a high variability, significant differences in NA among the strains were found in response of light and dark cultivation. Relat...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931606
更新日期:2004-01-01 00:00:00
abstract::The characterization of 11- and 18-residue peptaibols (peptides synthesized by peptide synthetases) at Trichoderma harzianum CECT 2413 (a filamentous fungus) was performed. Using a heterologous probe from tex1, the only peptaibol synthetase cloned and characterized so far in Trichoderma species, was cloned; a region t...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02932165
更新日期:2006-01-01 00:00:00
abstract::Thirty-three fully breast-fed infants aged between 1 and 12 weeks were screened for bifidobacteria in feces. Bifidobacteria counts in most fecal samples determined both by TPY agar and FISH procedure ranged from 10(8) to 10(11) CFU/g. Three infants did not contain any bifidobacteria in their fecal samples. One child w...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931404
更新日期:2004-01-01 00:00:00
abstract::The highest level of secreted brefeldin A was present in glucose-grown cultures, intermediate levels in glucose-fructose, and xylose cultures and low levels in fructose- and galactose-grown cultures of Curvularia lunata. The biomass decreased from glucose, fructose, xylose, glucose-fructose to galactose cultures. Bref...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02876993
更新日期:1977-01-01 00:00:00
abstract::Twenty-one healthy bottle-fed infants were screened monthly (1-4 months) for bifidobacteria in their stools. Bifidobacteria were detected by culture and isolates specified by PCR. Alternatively, direct PCR in undiluted fecal suspensions was carried out for detection of bifidobacteria under the cultural detection level...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931594
更新日期:2006-01-01 00:00:00
abstract::The antifungal effect of 2-alkylthio-6-amino- and 2-alkylthio-6-formamidobenzothiazoles (22 derivatives in all) on Aspergillus niger and various Candida yeasts was tested. No significant effect was observed with A. niger. With the pathogenic yeast species C. albicans and C. guilliermondii, the most efficient derivativ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02814461
更新日期:1989-01-01 00:00:00
abstract::Mitochondria isolated from Neurospora crassa were purified by centrifugation in a Percoll density gradient. Enzyme activities and cytochrome differential spectra revealed a high purity of the mitochondria. As compared with a crude mitochondrial fraction the purified mitochondria exhibited a high respiratory activity a...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02879491
更新日期:1983-01-01 00:00:00
abstract::Ten thermophilic Gram-positive bacteria were isolated from various soils of Saudi Arabia. The strains are spore-forming rods belonging to the species Bacillus stearothermophilus. The cells are motile, strictly aerobic, catalase and oxidase positive. The sporangia appear to be swollen and their position varies from ter...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02927328
更新日期:1981-01-01 00:00:00
abstract::Submerged cultures of the low-production strain of Tolypocladium inflatum DSM 63544 formed a mixture of cyclosporins (CS) consisting of CS-A, CS-B, "CS-3" and "CS-4". Glucose, sucrose and maltose were highly favored for biomass production but provided a different physiological state necessary for CS biosynthesis. Not ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02884035
更新日期:1991-01-01 00:00:00
abstract::Using phenol--water extraction, a lipopeptidopolysaccharide complex (LPPS) was isolated from Listeria monocytogenes. Some biological and immunological properties of LPPS were compared with lipopolysaccharide isolated by the same procedure from Salmonella typhimurium. LPPS possesses low pyrogenicity, but the immunologi...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02923515
更新日期:1985-01-01 00:00:00
abstract::Coxiella burnetii (C. burnetii), an intracellular zoonotic bacterium causing Q fever, occurs widely in cattle herds. After invasion of the pregnant uterus and initial localization in the placenta, active C. burnetii infections may spread to the fetus hematogenously or by the amniotic-oral route and thus may cause abor...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-017-0553-4
更新日期:2018-03-01 00:00:00
abstract::The possibility of utilizing derivatives of 4-methylumbelliferone (4-MUBF) for the determination of activity of enzymes from the group of hydrolyses was tested in 20 strains of 12 species of mycobacteria. The method proved to be suitable especially for determination of arylsulphatase, beta-D-galactose and acid phospha...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02876698
更新日期:1980-01-01 00:00:00