Abstract:
:A hexokinase preparation was obtained from a Saccharomyces cerevisiae mutant strain deficient in glucosephosphate isomerase (GPI) and mannosephosphate isomerase (MPI) by precipitation with ammonium sulfate. The supernatant fraction corresponding to 40-60 % saturation showed the lowest content in GPI and MPI activity. The fraction was used without further purification in the determination of glucose, either free or in a mixture with fructose and mannose. The results were similar to those obtained with pure commercial hexokinase.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Cabrera V,Pascual C,Herrera LSdoi
10.1007/BF02927364subject
Has Abstractpub_date
1981-01-01 00:00:00pages
107-11issue
2eissn
0015-5632issn
1874-9356journal_volume
26pub_type
杂志文章abstract::White-rot fungi, Coriolus versicolor and Funalia trogii, produced laccase on media with diluted olive-oil mill wastewater and vinasse. Addition of spent cotton stalks enhanced the laccase activity with a maximum after 12 d of cultivation. ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02873591
更新日期:2001-01-01 00:00:00
abstract::Mitochondria isolated from Neurospora crassa were purified by centrifugation in a Percoll density gradient. Enzyme activities and cytochrome differential spectra revealed a high purity of the mitochondria. As compared with a crude mitochondrial fraction the purified mitochondria exhibited a high respiratory activity a...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02879491
更新日期:1983-01-01 00:00:00
abstract::A selection of tert-butylhydroperoxide (tBOOH)-tolerant Candida albicans mutants showed increased tolerances to 19 different stress conditions. These mutants are characterized by a constitutively upregulated antioxidative defense system and, therefore, adaptation to oxidative stress may play an important role in gaini...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-014-0305-7
更新日期:2014-07-01 00:00:00
abstract::Streptococcus dysgalactiae subsp. equisimilis (SDSE), belonging to the group C and G streptococci, are human pathogens reported to cause clinical manifestations similar to infections caused by Streptococcus pyogenes. To scrutinize the distribution of gene coding for S. pyogenes virulence factors in SDSE, 255 isolates ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-018-0595-2
更新日期:2018-09-01 00:00:00
abstract::Virus-like particles (VLPs) were isolated from the yeast Wickerhamia fluorescens strain CCY61-1-1. The VLPs are approximately 42 nm in diameter and contain only one species of dsRNA molecule. The apparent length of the dsRNA determined by native agarose gel electrophoresis was 4.6 kbp. Analysis of protein content of t...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02814620
更新日期:1996-01-01 00:00:00
abstract::Five arsenic-resistant bacterial strains (designated MP1400, MP1400a, MP1400d, APSLA3, and BPSLA3) were isolated from soils collected at the Alps region (Italy), which showed no contamination by arsenic. Phylogenetic analysis of the 16S rRNA gene sequences assigned them to the genera Pseudomonas and Bacillus. Bacillus...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-011-0010-8
更新日期:2011-01-01 00:00:00
abstract::Staphylococcus aureus is a leading cause of bloodstream infections (BSI) and diseases that may be caused by hematogenous spread. The staphylococcal adhesin, for which the association with the infections emerging as a complication of septicemia has been well documented, is a bone sialoprotein-binding protein (Bbp). The...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-014-0321-7
更新日期:2014-11-01 00:00:00
abstract::In Slovakia, dairy products made from ewes' milk have a long tradition. These products include the lactic acid product called "žinčica" which is a by-product occurring during the preparation of ewes' lump cheese. There is no information in the literature regarding the special properties of the microbiota, especially l...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-019-00703-5
更新日期:2020-02-01 00:00:00
abstract::Virulence factors were compared in 15 Aeromonas spp. isolated from faeces of patients with Aeromonas-associated gastroenteritis and in 81 strains isolated from food. Strains from food did not show differences in the distribution of virulence factors when compared with strains isolated from faeces. However, 88.8% of Ae...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02814323
更新日期:1994-01-01 00:00:00
abstract::When investigating the effect of aeration on the utilization of a lipid substrate from the cultivation broth and production of salinomycin in Streptomyces albus it was demonstrated that a higher aeration results in a better utilization of soya oil and a higher production of the antibiotic. ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02879490
更新日期:1983-01-01 00:00:00
abstract::Vinasse, a recalcitrant waste of the ethanol industry was employed for the production of polyhydroxyalkanoate (PHA) by the extremely halophilic archaeon, Haloarcula marismortui in shake flasks. The PHA was recovered by osmotic lysis of the cells and subsequent purification by sodium hypochlorite and organic solvents. ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-011-0092-3
更新日期:2012-01-01 00:00:00
abstract::Regulatory mechanism of primary metabolism responsible for the biosynthesis of peptide ergot alkaloids was proposed on the basis of experimental results from the production phase of Claviceps purpurea (FR.) Tul. in parasitic and saprophytic cultures. The production-phase metabolism is characterized by uncoupling of gl...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02927365
更新日期:1981-01-01 00:00:00
abstract::One hundred clinical isolates of Pseudomonas aeruginosa were checked for their sensitivity towards silver nitrate. Majority of the isolates were resistant at 20 mg/L and the resistance decreased with increasing concentration of silver nitrate, only 5% of the organisms showed resistance above 70 mg/L. These silver-resi...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02820752
更新日期:1989-01-01 00:00:00
abstract::Lengths of permuted regions in the P22 and L phage genomes were estimated from the relative yields of DNA in many electrophoretic bands obtained using several restriction endonucleases. It was found that 3.6 kb (8.7%) of P22-DNA and 7.2 kb (17.8%) of L-DNA were circularly permuted. In both phages the sequential packag...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02933145
更新日期:1992-01-01 00:00:00
abstract::Pseudomonas aeruginosa is an opportunistic pathogen that causes serious infections, especially in patients with immunodeficiency. It exhibits multiple mechanisms of resistance, including efflux pumps, antibiotic modifying enzymes and limited membrane permeability. The primary reason for the development of novel therap...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-014-0333-3
更新日期:2015-01-01 00:00:00
abstract::A total of 152 lactic acid bacteria (LAB) were isolated from pickles produced in the Ankara-Çubuk region. These isolates were clustered into eight groups on the basis of their phenotypic characteristics including cell morphology, CO2 production from glucose, growth at 10 and 45 °C, growth in 6.5 % NaCl, and growth at ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-014-0363-x
更新日期:2015-05-01 00:00:00
abstract::The level of an antibiotic capable of inhibiting the etiological agent at the site of infection is an essential prerequisite for successful antibiotic therapy. In some cases, locally applied antibiotics may compensate for limitations of systemic administration and shorten systemic therapy. We aimed at verifying to wha...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-011-0093-2
更新日期:2012-09-01 00:00:00
abstract::In ostriches and pheasants, there is still limited information relating to staphylococci and their properties. Biogenic amines (BAs) are nitrogenous low-molecular-weight substances with biological functions in animals, plants and microorganisms. In this study, we focused on BA production by targeted faecal staphylococ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-017-0492-0
更新日期:2017-05-01 00:00:00
abstract::Plenty of metagenomic studies have suggested possible associations between microbiome composition and colorectal cancer (CRC). However, these techniques are not economic enough for routine use so far. Therefore, we explored the possibility to detect species associated with colorectal cancer by conventional culture fro...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-020-00807-3
更新日期:2020-12-01 00:00:00
abstract::The kinetics of extracellular neutral proteinase synthesis by an isogenic stringent (IS58) and a relaxed (IS56) strain of B. subtilis were compared. The specific enzyme formation rate by the stringent strain was higher than that of the relaxed one. Norvaline addition (1 mg/mL) induced the formation of pppGpp and ppGpp...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02883234
更新日期:1987-01-01 00:00:00
abstract::Candida bracarensis is an emerging cryptic species within the Candida glabrata clade. To date, little is known about its epidemiology, virulence, and antifungal susceptibility. This study documents the occurrence of C. bracarensis for the first time in Mexico and focuses on its in vitro production of hydrolytic enzyme...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-018-0592-5
更新日期:2018-07-01 00:00:00
abstract::In the present study, the cDNA sequences of Hsp70 and Hsp90 genes of Isaria farinosa (designated IFHSP70 and IFHSP90) were cloned and characterized using multiple techniques of molecular biology and bioinformatics. The genetic differentiation of the two genes was investigated among 10 geographically separated populati...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-018-00676-x
更新日期:2019-07-01 00:00:00
abstract::The effect of dental plaque bacteria on LFA-1 beta expression on peripheral blood leukocytes was studied in 20 patients with early-onset periodontitis and in 10 healthy controls. Stimulation of PMN with selected dental plaque bacteria which play a role in the pathogeny of periodontitis significantly increased the expr...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02815697
更新日期:1996-01-01 00:00:00
abstract::Data on the production of clostridial toxins in dialyzed cultures are summarized. Principal modifications of this cultivation technique suitable for both research and production are shown. If toxins are released from the cells by autolysis (neurotoxins of Clostridium tetani, C. botulinum; lethal factors of C. novyi an...
journal_title:Folia microbiologica
pub_type: 杂志文章,评审
doi:10.1007/BF02928083
更新日期:1988-01-01 00:00:00
abstract::Some Bacillus subtilis mutants with different levels of homoserine dehydrogenase were described. Strains that do not accumulate methionine have a high homoserine dehydrogenase activity. Low activity was detected in mutants where cell growth was completely inhibited by 0.7 mmol/L methionine. A low concentration of dime...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02884064
更新日期:1991-01-01 00:00:00
abstract::Fibrobacter succinogenes is a major cellulolytic species in the rumen. On the basis of molecular data, this species can be classified into four phylogenetic groups. Recently gathered ecological and physiological data have revealed the importance of this species, particularly phylogenetic group 1, in rumen fiber digest...
journal_title:Folia microbiologica
pub_type: 杂志文章,评审
doi:10.1007/s12223-008-0024-z
更新日期:2008-01-01 00:00:00
abstract::The production of bacterial enzymes in batch fermentations is compared with results obtained in continuous culture. When studying the production of alpha-amylase in Bacillus subtilis it was found that instability of the enzyme synthesis was due to nonhomogeneity of the population rather than to "the culture's history"...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02883137
更新日期:1982-01-01 00:00:00
abstract::Biodegradation of two amphiphilic "soft" antimicrobially active derivatives of lauric (dodecanoic) acid, a quaternary ammonium salt and an amine oxide bearing an amide or ester group, was followed using microorganisms from activated sludge. Primary biodegradation was determined by ion-selective electrodes, total biode...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02814548
更新日期:1993-01-01 00:00:00
abstract::Phosphatidylcholine is one of the most frequent phospholipid components of the inner mitochondrial membrane of Neurospora crassa. Quantitative analysis of phosphlipids of the wild strain of Neurospora crassa and of its two cho mutants showed that these strains did not significantly differ in the content of phosphatidy...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02879763
更新日期:1982-01-01 00:00:00
abstract::Glycerol-based medium (BM) with and without the addition of 1 g/L ascorbic acid (Asc) and/or 100 mg/L (+/-)-catechin (Cat) was tested for the storage of three commercial wine yeasts at -20 degrees C. The medium supplemented with Asc was also used to store 706 strains to verify the maintenance of the liquid state. A de...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-009-0036-3
更新日期:2009-01-01 00:00:00