A simple procedure to obtain yeast hexokinase free of glucosephosphate isomerase and mannosephosphate isomerase.

Abstract:

:A hexokinase preparation was obtained from a Saccharomyces cerevisiae mutant strain deficient in glucosephosphate isomerase (GPI) and mannosephosphate isomerase (MPI) by precipitation with ammonium sulfate. The supernatant fraction corresponding to 40-60 % saturation showed the lowest content in GPI and MPI activity. The fraction was used without further purification in the determination of glucose, either free or in a mixture with fructose and mannose. The results were similar to those obtained with pure commercial hexokinase.

journal_title

Folia microbiologica

authors

Cabrera V,Pascual C,Herrera LS

doi

10.1007/BF02927364

subject

Has Abstract

pub_date

1981-01-01 00:00:00

pages

107-11

issue

2

eissn

0015-5632

issn

1874-9356

journal_volume

26

pub_type

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