Characterization of five newly isolated bacteriophages active against Pseudomonas aeruginosa clinical strains.

Abstract:

:Pseudomonas aeruginosa is an opportunistic pathogen that causes serious infections, especially in patients with immunodeficiency. It exhibits multiple mechanisms of resistance, including efflux pumps, antibiotic modifying enzymes and limited membrane permeability. The primary reason for the development of novel therapeutics for P. aeruginosa infections is the declining efficacy of conventional antibiotic therapy. These clinical problems caused a revitalization of interest in bacteriophages, which are highly specific and have very effective antibacterial activity as well as several other advantages over traditional antimicrobial agents. Above all, so far, no serious or irreversible side effects of phage therapy have been described. Five newly purified P. aeruginosa phages named vB_PaeM_WP1, vB_PaeM_WP2, vB_PaeM_WP3, vB_PaeM_WP4 and vB_PaeP_WP5 have been characterized as potential candidates for use in phage therapy. They are representatives of the Myoviridae and Podoviridae families. Their host range, genome size, structural proteins and stability in various physical and chemical conditions were tested. The results of these preliminary investigations indicate that the newly isolated bacteriophages may be considered for use in phagotherapy.

journal_title

Folia microbiologica

authors

Kwiatek M,Mizak L,Parasion S,Gryko R,Olender A,Niemcewicz M

doi

10.1007/s12223-014-0333-3

subject

Has Abstract

pub_date

2015-01-01 00:00:00

pages

7-14

issue

1

eissn

0015-5632

issn

1874-9356

journal_volume

60

pub_type

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