Preparation of highly purified mitochondria of Neurospora crassa on a Percoll gradient.

Abstract:

:Mitochondria isolated from Neurospora crassa were purified by centrifugation in a Percoll density gradient. Enzyme activities and cytochrome differential spectra revealed a high purity of the mitochondria. As compared with a crude mitochondrial fraction the purified mitochondria exhibited a high respiratory activity and a fine ADP/O ratio. Electrophoresis of nucleic acids demonstrated the absence of cytoplasmic rRNA.

journal_title

Folia microbiologica

authors

Fecíková H,Turna J,Baráth Z

doi

10.1007/BF02879491

subject

Has Abstract

pub_date

1983-01-01 00:00:00

pages

409-13

issue

5

eissn

0015-5632

issn

1874-9356

journal_volume

28

pub_type

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