Abstract：
:The present study was designed to characterize six Trueperella (T.) abortisuis strains, cultured over a period of 5 months from fetus and abortion material of six pigs of a single farm in Mecklenburg-West Pomerania federal state, Germany. It was of interest to investigate the epidemiological relationships of the six strains among each other and whether a single bacterial clone was responsible for the abortion situation of the single farm. All six strains were identified phenotypically, by MALDI-TOF MS analysis and by phylogenetic analysis based on 16S rRNA gene and gap (encoding the glyceraldehyde 3-phosphate dehydrogenase) and tuf (encoding elongation factor tu) gene sequencing. Further genotypic comparison was performed using different genomic DNA fingerprint methods including BOX-PCR, (GTG)5-PCR, and three RAPD-PCRs. The sequence analysis of the genes gap and tuf and the genomic DNA fingerprinting results revealed, as noval findings, that the six T. abortisuis strains cultured from a single farm represent six different bacterial clones showing a genetic variability of this bacterial species in the pig population. All six T. abortisuis strains were isolated in mixed culture with several other bacterial species. However, the T. abortisuis strain, generally found in high numbers, seemed to be responsible for the abortion situation in the farm.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Alssahen M,Hassan AA,Wickhorst JP,Sammra O,Lämmler C,Glaeser SP,Kämpfer P,Timke M,Prenger-Berninghoff E,Abdulmawjood Adoi
10.1007/s12223-019-00753-9subject
Has Abstractpub_date
2020-06-01 00:00:00pages
491-496issue
3eissn
0015-5632issn
1874-9356pii
10.1007/s12223-019-00753-9journal_volume
65pub_type
杂志文章abstract：:This is the first report describing precipitation of bacteriophage particles with zinc chloride as a method of choice to isolate infectious lytic bacteriophages against Pectobacterium spp. and Dickeya spp. from environmental samples. The isolated bacteriophages are ready to use to study various (ecological) aspects of...
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journal_title：Folia microbiologica
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pub_type： 杂志文章
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doi：10.1007/BF02931528
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