Abstract：
:A total of 20 putative strains of Erwinia amylovora originating from 11 samples of host plants with symptoms of fire blight were analyzed in detail using commercial polyclonal antibodies in immunochemical tests. Fourteen strains reacted negatively in all tests; 6 strains reacted positively with a polyclonal antibody for PTA-ELISA (plate-trapped antigen-enzyme linked immunosorbent assay) at a concentration corresponding to A620 = 0.1, while at A620 readings of 0.01 and 0.001 the results were negative. Five strains reacted positively with a polyclonal antibody for indirect immunofluorescence test at all tested concentrations. Three of those strains were positive in the PCR test with AMSbL and AMSbR primers designed for detection of E. amylovora. In hypersensitivity test in tobacco and in immature pear fruit assay, all putative strains were negative while a known reference strain of E. amylovora gave a typical hypersensitive-reaction response. On a medium with 5% sucrose the reference strain of E. amylovora produced levan while putative strains did not. After modification of the PCR protocol, 3 putative strains reacted as negatives. Optimization of PCR test was achieved by finding the optimum annealing temperature and time for primers. The recommended annealing temperature (49 degrees C) for these primers was increased to 55 degrees C and the annealing time was reduced from 2 min to 30 s. Using the microbial identification system Biolog those 3 strains were identified as Pantoea dispersa (1 strain) and Pantoea agglomerans (2 strains). The strains are supposed to be white variants of the species P. dispersa and P. agglomerans occurring less frequently than the yellow variants. Since there were positive reactions in our immunochemical tests these strains could cause false positives in routine screening of plant samples.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Kokosková B,Mráz Idoi
10.1007/BF02931569keywords:
subject
Has Abstractpub_date
2005-01-01 00:00:00pages
217-21issue
3eissn
0015-5632issn
1874-9356journal_volume
50pub_type
杂志文章abstract：:Genetic diversity of 89 isolates of Rhizoctonia solani isolated from different pulse crops representing 21 states from 16 agro-ecological regions of India, 49 morphological, and 7 anastomosis groups (AGs) was analyzed using 12 universal rice primers (URPs), 22 random amplified polymorphic DNA (RAPD), and 23 inter-simp...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-012-0165-y
更新日期：2012-11-01 00:00:00
abstract：:The prevalence of potential virulence markers was determined among the population of Polish Campylobacter jejuni and Campylobacter coli isolates from children, chickens, pigs and dogs. The presence of the flaA, flaB, cdtA, cdtB, cdtC, cdtABC, virB11, and cj0588 genes among 74 C. jejuni and 15 C. coli isolates was dete...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-010-0025-6
更新日期：2010-03-01 00:00:00
abstract：:Lengths of permuted regions in the P22 and L phage genomes were estimated from the relative yields of DNA in many electrophoretic bands obtained using several restriction endonucleases. It was found that 3.6 kb (8.7%) of P22-DNA and 7.2 kb (17.8%) of L-DNA were circularly permuted. In both phages the sequential packag...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02933145
更新日期：1992-01-01 00:00:00
abstract：:The majority of antibiotics and substances with diverse biological activity used in medicine are produced by actinomycetes, nonfilamentous bacteria and fungi. Other microorganisms, such as myxobacteria, pseudomonads, nocardias, basidiomycetes, marine microorganisms, enterobacteria, halobacteria, hyperthermophiles etc....
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/BF02993460
更新日期：2003-01-01 00:00:00
abstract：:The potentiality of twenty-five fungal species belonging to 14 genera isolated from Egyptian soils to produce mevinolin, a hypocholesterolemic agent, when grown on selected substrates was tested. For the first screening samples of culture filtrates were tested by TLC and the positive results were further estimated by ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02826557
更新日期：1997-01-01 00:00:00
abstract：:NaN3 was found to inhibit transformation but not the irreversible binding of donor 3H-DNA in competent cells of the original low-transformable strain Bacillus subtilis 168 trp2. Addition of NaN3 to cells of two mutants Bacillus subtilis HT39 and HT46 with an increased transformability decreased substantially the irrev...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02877669
更新日期：1977-01-01 00:00:00
abstract：:This study presents the effects of the probiotic and enterocin M-producing strain Enterococcus faecium AL41 on microbiota, phagocytic activity (PA), oxidative stress, performance and biochemical parameters when applied individually or in combination with Eleutherococcus senticosus in rabbits. The novelty of the study ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-015-0423-x
更新日期：2016-03-01 00:00:00
abstract：:The color of light (white, red, blue, and green) had a significant effect on the growth and reproductive processes (both in the nucleocytoplasmic and chloroplast compartment of the cells) in synchronous cultures of Scenedesmus obliquus. This effect decreased in the order red > white > blue > green. In the same order, ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931828
更新日期：2006-01-01 00:00:00
abstract：:The pathogenesis of bacillary dysentery can be studied at different levels of integration of the cellular components that constitute the colonic mucosal barrier. We considered the interaction of Shigella flexneri in three experimental systems that provide complementary information and a scheme of events occurring in h...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/BF02818608
更新日期：1998-01-01 00:00:00
abstract：:Strains of Rhodospordium torloides produced two peptides into the culture filtrate. Peptide I contained 6 amino acids and peptide II contained 5 amino acids. The peptides however, are, not homologous to the alpha-factor, and become assimilated at a later phase of growth. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02876614
更新日期：1980-01-01 00:00:00
abstract：:Cryptococcus neoformans is encapsulated yeast that causes cryptococcosis. The cryptococcal meningitis may cause neuropsychiatric symptoms. Here, we evaluated the in vitro activity of amphotericin B (AMB), chlorpromazine (CLOR), and sertraline (SERT) alone or in combination against clinical isolates of C. neoformans co...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-016-0449-8
更新日期：2016-09-01 00:00:00
abstract：:Cardiolipin synthesis was studied during the aerobic synchronous growth of aerobically grown yeast Saccharomyces cerevisiae. The time course of the synthesis was stepwise and the rise in cardiolipin level in cells coincided in time with the increase in cytochrome oxidase activity. This finding supports the notion of d...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02876990
更新日期：1977-01-01 00:00:00
abstract：:An experimental model in white mice, infected with a mildly virulent strain of Klebsiella pneumoniae, was elaborated for studies on local immunity of the respiratory tract. Instillation of klebsiella into the supralaryngeal space of anaesthetised animals proved to be more suitable than the commonly used method of intr...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02879494
更新日期：1983-01-01 00:00:00
abstract：:A total of 40 substances were tested for their inhibitory effect on the multiplication of a bacteriophage in a growing culture of Bacillus licheniformis and their influence on bacitracin production. Acriflavine was the only substance which, at a concentration of 3 microgram ml-1, completely suppressed phage multiplica...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02876597
更新日期：1978-01-01 00:00:00
abstract：:The ability of the rumen ciliates to utilize β-glucans other than cellulose and xylan is currently being recognized. The objective of the present study was to characterize the ability of the ciliate Diploplastron affine to digest some pachyman, laminarin, pustulan, curdlan and lichean. The protozoa were isolated from ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-012-0120-y
更新日期：2012-07-01 00:00:00
abstract：:Lactobacillus bulgaricus 59 and L. casei RTS and their respective nitrosoguanidine-induced mutants were estimated for titratable and volatile acidities, production of biacetyl and acetoin and for proteolytic activity. Both the mutants showed a marked increase in proteolytic activity as compared to the parents. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02897216
更新日期：1980-01-01 00:00:00
abstract：:Single chain Fv (scFv) antibodies (generated by phage display technology, molecules representing new and efficient tools in the research and diagnostics of infectious diseases) against the capsid protein (p25) of Maedi-Visna virus were selected. Several clones of p25 specific scFv antibodies were identified; one of th...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931380
更新日期：2003-01-01 00:00:00
abstract：:The uvrE (E. coli KS 114) cells carry a mutation in the gene that codes for helicase II. This is the protein responsible for replicative unwinding of double-helical DNA. The repair mode of such cells may be altered as compared with the wild type. The survival of uvrE cells during postirradiation incubation under inhib...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02922491
更新日期：1985-01-01 00:00:00
abstract：:Three indigenous plasmids designated pRK1, pRK2 and pRK3 were identified among producers of penicillin G acylase (PGA) derived from the strain Escherichia coli W ATCC 9637. Their size and copy number (CN) in E. coli W were determined (kb; CN): pRK1 (80; 3.4), pRK2 (5.1; 71), and pRK3 (4.8; 13.7). Strain E. coli RE2 ha...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02818544
更新日期：1999-01-01 00:00:00
abstract：:Mycobacterium avium paratuberculosis (Map) is a pathogen which causes a chronic progressive granulomatous enteritis known as paratuberculosis or Johne's disease and it primarily affects wild and domestic ruminants. The aim of this research was to examine a flock which consisted of 294 goats and was located in Garfagna...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-017-0518-7
更新日期：2017-11-01 00:00:00
abstract：:As a part of a program to develop starter cultures aiding in the spoilage control and sanitation of African fermented foods, a cereal-based food ('ogi' and its solid form 'agidi' or 'eko') was prepared using a bacteriocin-producing Lactobacillus strain as the starter culture. The survival of an enterotoxigenic Escheri...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02898642
更新日期：1997-01-01 00:00:00
abstract：:The gene encoding the coat protein (CP) of a potato virus Y (PVY) was cloned into expression vector pMPM-A4Omega. PVY CP was expressed in Escherichia coli and the purified recombinant protein was used for raising rabbit polyclonal antibodies. The sera and antibodies were tested for the detection of PVY in the laborato...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-008-0067-1
更新日期：2008-01-01 00:00:00
abstract：:An economic method for a rapid estimation of the number of copies of plasmid R6K delta 1 in E. coli cells using lysis of the cells directly on the agarose gel and electrophoretic separation of radioactively labelled plasmid and chromosomal DNA's is described. The method, particularly useful for screening purposes, per...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02879773
更新日期：1982-01-01 00:00:00
abstract：:The rosettes formed by mouse peritoneal macrophages or DCH-5 cells and TNP-erythrocytes coated with anti-TNP antibodies of different isotypes were inhibited to various extent by monosaccharides. The most effective inhibitors were N-acetylglucosamine, glucosamine, mannose and N-acetylneuraminic acid in 1-5 mmol/L conce...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814584
更新日期：1992-01-01 00:00:00
abstract：:The present paper reports a modified method for isolation of lysostaphin--a bacteriolytic agent with specific affinity for staphylococcal cell wall. The proposed purification scheme includes three steps. The first procedure is ultrafiltration through a membrane filter giving a yield of 75.6%. The result of ultrafiltra...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814386
更新日期：1993-01-01 00:00:00
abstract：:Inhibition of methanogenesis in ruminal cultures was attempted by hindering thiamine availability through its degradation by 'polyphenols' and competition for active sites on enzymes and transporters using thiamine structural analogs. Effects on fermentation were small and not consistently reversed by adding thiamine....
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-009-0075-9
更新日期：2009-11-01 00:00:00
abstract：:Four in vitro experiments were set up to verify the colonization potential of ectomycorrhizal (EcM) Cenococcum geophilum FR. (strain CGE-4), saprotrophic Geomyces pannorum (LINK) SIGLER & CARMICHAEL (GPA-1) and a frequent root-associated, potentially ericoid mycorrhiza (ErM)-forming Meliniomyces variabilis Hambleton &...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02932096
更新日期：2007-01-01 00:00:00
abstract：:Composition of sterol fractions of nystatin-resistant Candida maltosa strains was determined. Using UV-spectrometry, TLC and GLC-MS it was demonstrated that resistance to nystatin is connected with the composition alterations of yeast cell sterols. Block of different stages of ergosterol biosynthesis was revealed in s...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814494
更新日期：1991-01-01 00:00:00
abstract：:Propionate at concentrations up to 0.05% decreased at 25 degrees C the growth and sporulation of Penicillium aurantiogriseum. The standard size of conidiogenous structures (metulae, phialids) and conidia was diminished. The effect was more pronounced at a higher temperature (30 degrees C). Inhibition of ochratoxin pro...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814202
更新日期：1995-01-01 00:00:00
abstract：:Ultrastructural changes induced by Rickettsia slovaca standard type (ST) and wild type (WT) were examined during their life cycle in L929 and Vero cells. R. slovaca invaded the cytoplasm of the host cell by phagocytosis on the 1st d p.i. Rickettsiae adhering to the cytoplasmic membrane were engulfed by cellular extens...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-009-0019-4
更新日期：2009-01-01 00:00:00
