Modified simplified method for isolation of lysostaphin from the culture filtrate of Staphylococcus staphylolyticus.

Abstract:

:The present paper reports a modified method for isolation of lysostaphin--a bacteriolytic agent with specific affinity for staphylococcal cell wall. The proposed purification scheme includes three steps. The first procedure is ultrafiltration through a membrane filter giving a yield of 75.6%. The result of ultrafiltration is a concentrated, 10-times purified preparation of lysostaphin with specific activity 0.62 U/mg which can be used for digestion of S. aureus cells. Further step, performed by ion-exchange chromatography on DEAE-cellulose, yields a 60-times purified preparation containing a mixture of enzyme components of lysostaphin. The yield of this step is 47.2%, the preparation contains 3.54 U/mg protein. Using gel filtration on Sephadex G-50 a component with hexosaminidase activity was separated from the endopeptidase component on the basis of molar mass difference. A 270-times purified preparation of lysostaphin-endopeptidase with minimum of contaminating substances was obtained in this step. The yield of gel filtration was 22.1%, specific activity increased up to 16.3 U/mg protein.

journal_title

Folia microbiologica

authors

Márová I,Dadák V

doi

10.1007/BF02814386

subject

Has Abstract

pub_date

1993-01-01 00:00:00

pages

245-52

issue

3

eissn

0015-5632

issn

1874-9356

journal_volume

38

pub_type

杂志文章
  • Biological activity of secondary metabolites produced by a strain of Pseudomonas fluorescens.

    abstract::Biological activity of secondary metabolites produced by a plant-growth-promoting Pseudomonas fluorescens was evaluated. The strain produced antibiotics phenazine (PHE), 2,4-diacetylphloroglucinol (PHL) and siderophore pyoverdin (PYO) in standard King's B and succinic acid media, respectively. After extraction, PYO wa...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02818690

    authors: Boruah HP,Kumar BS

    更新日期:2002-01-01 00:00:00

  • Potential of the strain Raoultella sp. KDF8 for removal of analgesics.

    abstract::The bacterial strain KDF8 capable of growth in the presence of diclofenac and codeine analgesics was obtained after chemical mutagenesis of nature isolates from polluted soils. The strain KDF8 was identified as Raoultella sp. based on its morphology, biochemical properties, and 16S rRNA gene sequence. It was deposited...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/s12223-017-0563-2

    authors: Palyzová A,Zahradník J,Marešová H,Sokolová L,Kyslíková E,Grulich M,Štěpánek V,Řezanka T,Kyslík P

    更新日期:2018-05-01 00:00:00

  • Inhibition of lymphoproliferative response and its restoration with a glucan immunomodulator in mice with experimental larval toxocarosis.

    abstract::A high infective dose of Taxocara canis eggs (2,500 eggs per mouse) induced a partial immunosuppression in mice, manifested by inhibition of the proliferative response of splenic T and B cells to polyclonal activators. A glucan immunomodulator given to infected animals at the beginning of the experiment showed a marke...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02820794

    authors: Borosková Z,Reiterová K,Dubinský P,Tomasovicová O,Machnicka B

    更新日期:1998-01-01 00:00:00

  • Correction to: Diversity of culturable bacterial communities in the intestinal tracts of goldfish (Carassius auratus) and their ability to produce N-acyl homoserine lactone.

    abstract::In the original publication, the number of the NCTC strain used was published incorrectly. The authors apologize for the error. The error has no effect on the validity of the results and is corrected for accuracy. ...

    journal_title:Folia microbiologica

    pub_type: 杂志文章,已发布勘误

    doi:10.1007/s12223-017-0558-z

    authors: Sugita H,Kitao S,Narisawa S,Minamishima R,Itoi S

    更新日期:2018-01-01 00:00:00

  • Effect of pH on the denitrifying enzyme activity in pasture soils in relation to the intrinsic differences in denitrifier communities.

    abstract::The effects of pH on denitrifying enzyme activity (DEA) and on the ratio of the denitrification products, N(2)O and N(2), were determined in three pasture soils differing in cattle impact. The linkage between intrinsic differences in the denitrifying communities and pH effects on relative N(2)O production was also ass...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/s12223-011-0045-x

    authors: Cuhel J,Simek M

    更新日期:2011-05-01 00:00:00

  • Species identification of strains belonging to genus Citrobacter using the biochemical method and MALDI-TOF mass spectrometry.

    abstract::Strains of genus Citrobacter (152 isolates from 1950 to 1988 deposited in the Czech National Collection of Type Cultures, Prague) were re-classified using biological and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) methods. One-hundred thirty-six strains (ca. 90 %) were i...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/s12223-014-0340-4

    authors: Kolínská R,Spanělová P,Dřevínek M,Hrabák J,Zemličková H

    更新日期:2015-01-01 00:00:00

  • Hemolytic uremic syndrome caused by verotoxin-producing Escherichia coli O26. Case report.

    abstract::Verotoxin-producing Escherichia coli (VTEC) are nowadays among the most important emerging group of food-borne pathogens (VTEC strains cause gastroenteritis that can be complicated by the hemorrhagic colitis or hemolytic uremic syndrome, HUS). Escherichia coli 026 producing verotoxin 2 was isolated and its identity co...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02931454

    authors: Liptáková A,Siegfried L,Kmetová M,Birosová E,Kotulová D,Bencátová A,Kosecká M,Bánovcin P

    更新日期:2005-01-01 00:00:00

  • Streptococcal extracellular NAD+ nucleosidase. Characterization of changes occurring during purification.

    abstract::Purification of streptococcal extracellular NAD+ nucleosidase is associated with changes of kinetic properties. A high-molecular weight component is required for a full activity of the enzyme. The component is not produced by bacteria and is present in the Todd-Hewitt cultivation medium, the beef-heart extract serving...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02876396

    authors: Zahradník FJ

    更新日期:1980-01-01 00:00:00

  • Lowering of ochratoxin A level in milk by yoghurt bacteria and bifidobacteria.

    abstract::A decrease in the content of ochratoxin A (OA) was observed in milk samples fermented by yoghurt bacteria and bifidobacteria. OA was added to the milk before fermentation at a rate of 0.05-1.5 mg/L. No residues of OA were found in samples containing 0.05 and 0.1 mg/L of OA, fermented by S. Salivarius subsp. thermophil...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02816335

    authors: Skrinjar M,Rasić JL,Stojicić V

    更新日期:1996-01-01 00:00:00

  • Conversion of glutamate to glutamine by permeabilized Corynebacterium glutamicum.

    abstract::Glutamate was converted to glutamine by Corynebacterium glutamicum permeabilized by ethanol (10%). ATP was supplied to the reaction by dried Saccharomyces cerevisiae or regenerated by acetyl kinase. High glutamine synthetase activity in C. glutamicum was induced by cultivation of the microorganism in media containing ...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02816248

    authors: Behenský P,Stenzl V,Adámek F,Bĕhal V

    更新日期:1999-01-01 00:00:00

  • Cardiolipin synthesis during the cell cycle of the yeast Saccharomyces cervisiae.

    abstract::Cardiolipin synthesis was studied during the aerobic synchronous growth of aerobically grown yeast Saccharomyces cerevisiae. The time course of the synthesis was stepwise and the rise in cardiolipin level in cells coincided in time with the increase in cytochrome oxidase activity. This finding supports the notion of d...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02876990

    authors: Greksák M,Nejedlý K,Zborowski J

    更新日期:1977-01-01 00:00:00

  • Membrane proteins and squalene-hydrosqualene profile in methanoarchaeon Methanothermobacter thermautotrophicus resistant to N,N'-dicyclohexylcarbodiimide.

    abstract::The biochemical basis of a defective bioenergetic system was attempted to be determined in N,N'-dicyclohexylcarbodiimide (DCCD)-resistant mutant of Methanothermobacter thermautotrophicus. Components participating in the maintenance of methanoarchaeal membrane structure and function, such as the composition of the mixt...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/s12223-008-0034-x

    authors: Nováková Z,Surín S,Blasko J,Majerník A,Smigán P

    更新日期:2008-01-01 00:00:00

  • Protein overexport in a Saccharomyces cerevisiae mutant is not due to facilitated release of cell-surface proteins.

    abstract::Saccharomyces cerevisiae strain MW11 is a temperature-sensitive mutant which exports twenty times more proteins at 37 degrees C than parental or wild-type strains do. To understand the mechanism underlying the protein overexport in the mutant the possibility of an altered cell-wall structure leading to facilitated rel...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02908954

    authors: Alexieva KI,Venkov PV

    更新日期:2000-01-01 00:00:00

  • Production of extracellular lysine by a strain of Bacillus coagulans. Identification and requirements for growth and lysine accumulation.

    abstract::A naturally deficient thiamine and methionine requiring strain of Bacillus coagulans (Ms 5) accumulates lysine in medium only when exogenous pyridoxine (optimal concentration, 0.1 mu g/ml) are supplied. Threonine exerts an inhibitory effect at higher concentrations but pyridoxine does not. ...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02877085

    authors: Chatterjee S,Banerjee AK

    更新日期:1975-01-01 00:00:00

  • Local distribution of ectomycorrhizae-associated basidiomycetes in forest soil correlates with the degree of soil organic matter humification and available electrolytes.

    abstract::Spatial distribution of ectomycorrhizae-associated basidiomycetes was determined in oakbirch forest using terminal restriction fragment length polymorphism (T-RFLP) analysis. The data were correlated with actual soil humidity, pH, electric conductivity of the soil extract, absorbance A(465) and A(665) of water and alk...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/s12223-010-0076-8

    authors: Gryndler M,Soukupová L,Gryndlerová H,Baldrian P,Hršelová H

    更新日期:2010-09-01 00:00:00

  • Antifungal efficacy of bacteria isolated from marine sedentary organisms.

    abstract::The antibiotic-producing ability of 57 bacteria isolated from 8 marine sedentary organisms, 6 sponges (Spirastrella sp., Phyllospongia sp., Ircinia sp., Aaptos sp., Azorica sp., Axinella sp.), 1 soft coral (Lobophytum sp.) and 1 alga (Sargassum sp.), was evaluated against 6 phytopathogenic fungi (Helminthosporium oryz...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02818565

    authors: Mohapatra BR,Bapuji M,Sree A

    更新日期:2002-01-01 00:00:00

  • Colocalization of cortical microtubules and F-actin in Dipodascus magnusii using confocal laser scanning microscopy.

    abstract::Distribution of microtubules and F-actin in aerobically growing cells of Dipodascus magnusii, belonging to the class Saccharomycetes was analyzed using immunofluorescence microscopy and labeling with rhodamine-tagged phalloidin. A conspicuous system of permanent cytoplasmic microtubules was observed in association wit...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02930952

    authors: Hasek J,Trachtulcová P,Kohlwein SD,Streiblová E

    更新日期:2003-01-01 00:00:00

  • Biotransformation of 1,8-cineole by solid-state fermentation of Eucalyptus waste from the essential oil industry using Pleurotus ostreatus and Favolus tenuiculus.

    abstract::Biotechnological conversion of low-cost agro-industrial by-products, such as industrial waste or terpenes from the distillation of essential oils from plants into more valuable oxygenated derivatives, can be achieved by using microbial cells or enzymes. In Argentina, the essential oil industry produces several tons of...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/s12223-015-0422-y

    authors: Omarini A,Dambolena JS,Lucini E,Jaramillo Mejía S,Albertó E,Zygadlo JA

    更新日期:2016-03-01 00:00:00

  • The influence of Lactobacillus acidophilus-derived surfactants on staphylococcal adhesion and biofilm formation.

    abstract::The ability of surfactants obtained from three Lactobacillus acidophilus strains to inhibit Staphylococcus aureus and S. epidermidis biofilms was evaluated. Their influence was determined on bacterial initial adhesion, biofilm formation and dispersal using MTT-reduction assay, confocal laser scanning microscopy and im...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/s12223-008-0009-y

    authors: Walencka E,Rózalska S,Sadowska B,Rózalska B

    更新日期:2008-01-01 00:00:00

  • Structure and function of the stalk, a putative regulatory element of the yeast ribosome. Role of stalk protein phosphorylation.

    abstract::The ribosomal stalk is involved directly in the interaction of the elongation factors with the ribosome during protein synthesis. The stalk is formed by a complex of five proteins, four small acidic polypeptides and a larger protein which directly interacts with the rRNA at the GTPase center. In eukaryotes, the acidic...

    journal_title:Folia microbiologica

    pub_type: 杂志文章,评审

    doi:10.1007/BF02816234

    authors: Rodriguez-Gabriel MA,Bou G,Briones E,Zambrano R,Remacha M,Ballesta JP

    更新日期:1999-01-01 00:00:00

  • Degradation of wood by the basidiomycete Coriolopsis occidentalis.

    abstract::Chemical and microscopic features of wood decay by the basidiomycete Coriolopsis occidentalis are described. The fungus was grown on blocks of poplar, oak, and fir wood and caused significant mass, lignin, and saccharide losses in all kinds of wood. Poplar wood was particularly strongly affected. Twelve weeks after in...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02814527

    authors: Homolka L,Nerud F,Kofronová O,Novotná E,Machurová V

    更新日期:1994-01-01 00:00:00

  • Comparative study of the life cycle dependent post-translation modifications of protein synthesis elongation factor Tu present in the membrane proteome of streptomycetes and mycobacteria.

    abstract::We present the results of analysis of membrane phosphoproteomes from individual morphological stages of Streptomyces coelicolor that reflect developmentally dependent heterogeneity and phosphorylation of intrinsic and externally added purified Strepomyces aureofaciens EF-Tu. Fast growing nonpathogenic Mycobacterium sm...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/s12223-010-0029-2

    authors: Holub M,Bezousková S,Petrácková D,Kalachová L,Kofronová O,Benada O,Weiser J

    更新日期:2010-05-01 00:00:00

  • Microflora of the honeybee gastrointestinal tract.

    abstract::Microorganisms in the midgut and rectum of the honeybee were enumerated and characterized. Counts of aerobic microorganisms were distinctly lower than counts of anaerobes (10(5)-10(6) viable cells per g of intestinal content vs. 10(8)-10(9) per g). Total numbers of anaerobic microorganisms were almost identical with t...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02931394

    authors: Kacániová M,Chlebo R,Kopernický M,Trakovická A

    更新日期:2004-01-01 00:00:00

  • The bactericidal activity of sera of healthy neonates and of newborns with hyperbilirubinaemia to Escherichia coli.

    abstract::Cord and newborn sera were found to possess a significantly decreased in vitro bactericidal activity to Escherichia coli as compared to sera of adult individuals. Hyperbilirubinaemia in neonates suffering from both Rh and ABO incompatibility results in a further significant impairment of the bactericidal activity of t...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02927298

    authors: Miler I,Vondrácek J,Hromádková L

    更新日期:1979-01-01 00:00:00

  • Postantibiotic effects and postantibiotic sub-MIC effects of ciprofloxacin, pefloxacin and amikacin on the biological properties of Salmonella strains.

    abstract::The postantibiotic effect (PAE) and the postantibiotic sub-MIC effect (PASME) of ciprofloxacin, pefloxacin and amikacin were studied for Salmonella typhimurium and S. enteritidis strains. PAE was induced by 2 x and 4 x MIC of antibiotics studied for 0.5 h. After PAE and PASME their effect on prophage induction of a ly...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02816944

    authors: Majtán V,Majtánová L

    更新日期:1997-01-01 00:00:00

  • Different gut microbiome composition in obese Guizhou minipigs between female and castrated male.

    abstract::Gut microbiome lives in the intestinal tract of animals and plays an important role in almost all life processes. Gut microbiome balance is beneficial to health, and imbalance leads to many diseases, one of which is obesity epidemic. However, gut microbiome is also influenced by host hormone, and different gut microbi...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/s12223-019-00704-4

    authors: Yao G,Wu S,Zeng X,Zhao H,Wang G,Chen M,Qian N

    更新日期:2019-11-01 00:00:00

  • Aspergillus niger pH 2.1 optimum acid phosphatase with high affinity for phytate.

    abstract::An extracellular acid phosphatase isolated from the culture of a wild strain Aspergillus niger, producing the dephosphorylating 3-phytase, was obtained in a homogeneous form by sequential application of ultrafiltration through PS 50 membrane, gel filtration on Sephadex G-100 and ion exchange chromatography on DEAE-Sep...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02931618

    authors: Gargova S,Sariyska M,Angelov A,Stoilova I

    更新日期:2006-01-01 00:00:00

  • The K3 type killer strains of genus Saccharomyces for wine production.

    abstract::Using simultaneous fusion of protoplasts of three strains the auxotrophic K3 killer designated MH1 was prepared at a very low frequency and further employed for the transfer of the K3 killer character into a commercial wine yeast. A novel Saccharomyces Bratislava 1K3 strain with desirable technological and anti-yeast ...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02814566

    authors: Sulo P,Michalcáková S

    更新日期:1992-01-01 00:00:00

  • Effect of volatile substances from Origanum majorana and Ocimum basilicum on spore respiration and germination of some soil fungi.

    abstract::Oxygen uptake by the spores of Fusarium moniliforme, F. oxysporum, F. semitectum, F. solani, Mucor racemosus and Trichoderma viride was increased in the presence of volatile substances extracted from Origanum majorana and Ocimum basilicum. This increase was greater in the presence of volatile substances from O. basili...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02885580

    authors: Afifi AF,Dowidar AE

    更新日期:1978-01-01 00:00:00

  • Plant growth promotion and fungicidal activity in a siderophore-producing strain of Proteus sp.

    abstract::A proteus strain inhibited mycelial growth of Fusarium oxysporum in vitro. Seed bacterization showed significant plant growth promotion and Fusarium-wilt suppression activity of Phaseolus mungo in a gnotobiotic system. The culture filtrate of this strain exhibited three prominent bands in UV-VIS spectra between 300 an...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02818723

    authors: Barthakur M

    更新日期:2000-01-01 00:00:00