Abstract:
:Distribution of microtubules and F-actin in aerobically growing cells of Dipodascus magnusii, belonging to the class Saccharomycetes was analyzed using immunofluorescence microscopy and labeling with rhodamine-tagged phalloidin. A conspicuous system of permanent cytoplasmic microtubules was observed in association with multiple nuclei. In elongating cells, helices of cytoplasmic microtubules appeared at the cell cortex. In cells approaching cytokinesis transversely oriented microtubules were revealed at incipient division sites. Confocal laser scanning microscopy showed a continuity of these transverse microtubules with the remaining microtubule network. The actin system of D. magnusii consisted of patches and filaments. Patches were found to accumulate at the tips of growing cells. Bands of fine actin filaments were usually observed before F-actin rings were established. A close cortical association of microtubules with the F-actin ring was documented on individual optical sections of labeled cells. Cells with developing septa showed medial F-actin discs associated at both sides with microtubules. Colocalization of cytoplasmic microtubules with actin filaments at the cortex of dividing cells supports a role of both cytoskeletal components in controlling cell wall growth and septum formation in D. magnusii.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Hasek J,Trachtulcová P,Kohlwein SD,Streiblová Edoi
10.1007/BF02930952keywords:
subject
Has Abstractpub_date
2003-01-01 00:00:00pages
177-82issue
2eissn
0015-5632issn
1874-9356journal_volume
48pub_type
杂志文章abstract::The vacA and cagA genotypes of Helicobacter pylori exhibited distinct geographic distribution and correlation with severity of disease. In the above genotypes (obtained from 150 H. pylori-positive patients--139 with gastritis, 10 with ulcer and 1 patient with gastric cancer) combinations vacA s1/m1 and s2/m2 were dete...
journal_title:Folia microbiologica
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journal_title:Folia microbiologica
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journal_title:Folia microbiologica
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journal_title:Folia microbiologica
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journal_title:Folia microbiologica
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doi:10.1007/BF02815697
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journal_title:Folia microbiologica
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journal_title:Folia microbiologica
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doi:10.1007/BF02818544
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journal_title:Folia microbiologica
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journal_title:Folia microbiologica
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doi:10.1007/s12223-009-0064-z
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abstract::A comparative examination of reduced methyl [MV·](+) and benzyl [BV·](+) viologens (as artificial electron donors for quantitative estimation of the respiratory periplasmic (Nap) and membrane-embedded (Nar) nitrate reductases) using a newly constructed nap mutant strain of Paracocccus denitrificans was done. The activ...
journal_title:Folia microbiologica
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更新日期:2011-01-01 00:00:00
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journal_title:Folia microbiologica
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doi:10.1007/BF02818004
更新日期:2001-01-01 00:00:00
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更新日期:1976-01-01 00:00:00
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journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02815414
更新日期:1995-01-01 00:00:00
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journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02817670
更新日期:2002-01-01 00:00:00
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journal_title:Folia microbiologica
pub_type: 杂志文章
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journal_title:Folia microbiologica
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journal_title:Folia microbiologica
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doi:10.1007/BF02884088
更新日期:1983-01-01 00:00:00
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journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931569
更新日期:2005-01-01 00:00:00
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journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02877669
更新日期:1977-01-01 00:00:00
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doi:10.1007/BF02931828
更新日期:2006-01-01 00:00:00
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journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-012-0182-x
更新日期:2013-01-01 00:00:00
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journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931639
更新日期:2004-01-01 00:00:00
abstract::A new simple method used to eliminate polysaccharides that cause problems during DNA isolation was established for 6 different white-rot fungi using 1% hexadecyltrimethylammonium bromide (CTAB) as wash buffer and followed by centrifugation. Variation in the DNA yield and quality was ascertained using precipitating age...
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更新日期:2020-02-01 00:00:00
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journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02876678
更新日期:1978-01-01 00:00:00
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