Abstract：
:Due to the nature of Vibrio vulnificus infections (i.e., gastroenteritis and septicemia), only very few studies of a biofilm-associated form in this pathogen's life cycle have been conducted. We proposed that biofilm production by clinical strains of V. vulnificus would be higher than by environmental strains. Biofilm formation by clinical and environmental reference strains was tested under different temperatures (24, 30, and 37 °C), pH (5.5, 7.5, and 8.5) and iron concentrations (18, 30, 50, 100, and 200 μM). Biofilm production by clinical strains was consistently higher (p < 0.001) at 24 °C than by environmental strains. Higher biofilm production was observed at pH 5.5 by all strains. Growth rates were lowest at pH 5.5 for environmental strains but for clinical strains there were no differences at pH 5.5, 7.5, and 8.5, demonstrating a tolerance to acidic and alkaline conditions. There was a strong, direct correlation between iron concentration in the growth medium and biofilm production by all strains tested. The current study indicates that biofilm formation might be important for the survival of V. vulnificus in vivo as well as in the marine environment. With regard to temperature and pH, higher biofilm production appears to be a trait of clinical strains and could be considered a virulence determinant in V. vulnificus.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Çam S,Brinkmeyer Rdoi
10.1007/s12223-019-00761-9subject
Has Abstractpub_date
2020-06-01 00:00:00pages
557-566issue
3eissn
0015-5632issn
1874-9356pii
10.1007/s12223-019-00761-9journal_volume
65pub_type
杂志文章abstract：:A high infective dose of Taxocara canis eggs (2,500 eggs per mouse) induced a partial immunosuppression in mice, manifested by inhibition of the proliferative response of splenic T and B cells to polyclonal activators. A glucan immunomodulator given to infected animals at the beginning of the experiment showed a marke...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02820794
更新日期：1998-01-01 00:00:00
abstract：:Antibody response to major porin proteins of S. Typhi (OmpC and OmpF) was evaluated in sera of typhoid patients (culture positive, n = 28; Widal positive, n = 16). Sera from fever patients (n = 6) having etiology other than Salmonella, and normal healthy human controls (n = 18) were also included. No significant diffe...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-010-0086-6
更新日期：2010-09-01 00:00:00
abstract：:In this study, a method combining Raman spectroscopy with chemometric analysis was developed for detection of phage presence in raw milk and discrimination of Streptococcus thermophilus and Lactobacillus bulgaricus phages which are among the main phages causing problems in dairy industry. For this purpose, S. thermoph...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-018-0604-5
更新日期：2018-09-01 00:00:00
abstract：:The present paper reports a modified method for isolation of lysostaphin--a bacteriolytic agent with specific affinity for staphylococcal cell wall. The proposed purification scheme includes three steps. The first procedure is ultrafiltration through a membrane filter giving a yield of 75.6%. The result of ultrafiltra...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814386
更新日期：1993-01-01 00:00:00
abstract：:Mutants of Streptomyces coeruleorubidus, blocked in the biosynthesis of anthracycline antibiotics of the daunomycine complex, were isolated from the production strains after treatment with UV light, gamma-radiation, nitrous acid, and after natural selection; according to their different biosynthetic activity the mutan...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02876678
更新日期：1978-01-01 00:00:00
abstract：:Inactivation of seven strains derived from Escherichia coli B differing in their capacity to repair damage to their DNA (exc, pol, rec) after irradiation with far (254 nm) and middle and near (300 to 380 and 320--400 nm) UV light was investigated. The same bacterial strains were also used as hosts for the UV-irradiate...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02876690
更新日期：1980-01-01 00:00:00
abstract：:Mycobacterium avium paratuberculosis (Map) is a pathogen which causes a chronic progressive granulomatous enteritis known as paratuberculosis or Johne's disease and it primarily affects wild and domestic ruminants. The aim of this research was to examine a flock which consisted of 294 goats and was located in Garfagna...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-017-0518-7
更新日期：2017-11-01 00:00:00
abstract：:The plasmid R1drd-19 Km- and its derivative pON5300 were split by the PstI restriction enzyme; they differed in a 2.4 Mg/mol fragment which was present only in the pON5300. Both plasmids were present in 1-2 copies per chromosomal equivalent in E. coli JC5455 cells. The pON5300 is stable in its original mutagenized hos...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02879554
更新日期：1983-01-01 00:00:00
abstract：:The activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSP) as well as of succinate dehydrogenase (SDG), NADH dehydrogenase (NDG) and fumarate hydratase (FHT) were examined in relation to mitochondrial ultrastructure changes in Aspergillus niger exposed to N,N-bis(3-aminopropyl)dodecy...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931448
更新日期：2006-01-01 00:00:00
abstract：:Fungal cells of Aureobasidium pullulans ATCC 201253 were immobilized by entrapment in chitosan beads, and the immobilized cells were investigated for their ability to produce the polysaccharide pullulan using batch fermentation. The 1% chitosan-entrapped fungal cells were capable of producing pullulan for two cycles o...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-011-0048-7
更新日期：2011-07-01 00:00:00
abstract：:The gene encoding the coat protein (CP) of a potato virus Y (PVY) was cloned into expression vector pMPM-A4Omega. PVY CP was expressed in Escherichia coli and the purified recombinant protein was used for raising rabbit polyclonal antibodies. The sera and antibodies were tested for the detection of PVY in the laborato...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-008-0067-1
更新日期：2008-01-01 00:00:00
abstract：:Three methods were used for the isolation of fungi in the present investigation: the dilution-plate method, hair-baiting technique with horse hairs and the "exposed plate" method. Sabouraud's glucose agar at 28 degrees C was also used as isolation medium. 102 species and 2 species varieties belonging to 36 genera were...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02821282
更新日期：1990-01-01 00:00:00
abstract：:Inhibition of methanogenesis in ruminal cultures was attempted by hindering thiamine availability through its degradation by 'polyphenols' and competition for active sites on enzymes and transporters using thiamine structural analogs. Effects on fermentation were small and not consistently reversed by adding thiamine....
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-009-0075-9
更新日期：2009-11-01 00:00:00
abstract：:Among 18 tested strains of Claviceps spp., 7 produced significant amounts of exocellular polysaccharide (EPS). The maximum production of EPS was found in fermentation broth of Claviceps viridis. The kinetics of growth, substrate consumption, and EPS production in the batch, aerobic, submerged culture of this fungus we...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02993470
更新日期：2003-01-01 00:00:00
abstract：:Microorganisms in the midgut and rectum of the honeybee were enumerated and characterized. Counts of aerobic microorganisms were distinctly lower than counts of anaerobes (10(5)-10(6) viable cells per g of intestinal content vs. 10(8)-10(9) per g). Total numbers of anaerobic microorganisms were almost identical with t...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931394
更新日期：2004-01-01 00:00:00
abstract：:Ecosystems worldwide are exposed to pollutants connected to the industrial production of pharmaceuticals. The objective of this study was to study the composition and characteristics of the soil microbial communities that had been exposed to long-term selection pressure caused by the industrial production of penicilli...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-020-00819-z
更新日期：2020-12-01 00:00:00
abstract：:The effect of the newly synthesized phosphonic compound dibutyl 2-octylamino-2-propanephosphonate (DBOP) on the growth of the aquatic plant Spirodela oligorrhiza and stability of red blood cells (RBC) and planar lipid membranes (BLM) was studied to determine its physiological activity and, if possible, correlate this ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02908944
更新日期：2000-01-01 00:00:00
abstract：:Uropathogenic Escherichia coli (UPEC) isolates contain large genomic segments, termed pathogenicity islands (PAIs), that contribute to their virulence. A total of 150 UPEC and 50 commensal E. coli isolates from outpatients were investigated for antimicrobial susceptibility and the presence of eight PAI markers. One hu...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-015-0433-8
更新日期：2016-05-01 00:00:00
abstract：:The yellow-green alga Trachydiscus minutus (class Xanthophyta) was cultivated in a standard medium and in media without sulfur and nitrogen. Its yield after a 16-d cultivation reached 13 g dry mass per 1 L medium. The content of oligoenoic ('polyenoic') fatty acid (PUFA), i.e. eicosapentaenoic (EPA), was in excess of ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-010-0039-0
更新日期：2010-05-01 00:00:00
abstract：:Mathematical expressions were derived for the dilution rate and concentration of algae determining the maximum biomass production in a semicontinuous algal culture. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02926655
更新日期：1979-01-01 00:00:00
abstract：:Candidiases, infections caused by germination forms of the Candida fungus, represent a heterogeneous group of diseases from systemic infection, through mucocutaneous form, to vulvovaginal form. Although caused by one organism, each form is controlled by distinct host immune mechanisms. Phagocytosis by polymorphonuclea...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/BF02931313
更新日期：2007-01-01 00:00:00
abstract：:Strains of fungi imperfecti of genera Aspergillus and Rhizopus were tested for the ability to produce alpha-mannosidases. The most suitable alpha-mannosidase producer of a total of 20 strains under study was Aspergillus Ravus Link 69. The parameters studied during the cultivation included the growth rate expressed as ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02876433
更新日期：1978-01-01 00:00:00
abstract：:Two Bacillus cereus feather-degrading strains (23/1 and 6/2) were transformed using a recombinant plasmid p5.2 carrying the alkaline proteinase gene (aprE). A high level of the aprE gene expression was observed when the recombinant strains were grown on sporulation medium. The expression of the aprE gene proceeded dur...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-010-0004-y
更新日期：2010-01-01 00:00:00
abstract：:In ostriches and pheasants, there is still limited information relating to staphylococci and their properties. Biogenic amines (BAs) are nitrogenous low-molecular-weight substances with biological functions in animals, plants and microorganisms. In this study, we focused on BA production by targeted faecal staphylococ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-017-0492-0
更新日期：2017-05-01 00:00:00
abstract：:Many serious diseases caused by Staphylococcus aureus appear to be associated with biofilms. Therefore, we investigated the biofilm-forming ability of the methicillin-resistant S. aureus (MRSA) isolates collected from hospitalized patients. As many as 96 % strains had the ability to form biofilm in vitro. The majority...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-012-0175-9
更新日期：2013-01-01 00:00:00
abstract：:The color of light (white, red, blue, and green) had a significant effect on the growth and reproductive processes (both in the nucleocytoplasmic and chloroplast compartment of the cells) in synchronous cultures of Scenedesmus obliquus. This effect decreased in the order red > white > blue > green. In the same order, ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931828
更新日期：2006-01-01 00:00:00
abstract：:The kinetics of extracellular neutral proteinase synthesis by an isogenic stringent (IS58) and a relaxed (IS56) strain of B. subtilis were compared. The specific enzyme formation rate by the stringent strain was higher than that of the relaxed one. Norvaline addition (1 mg/mL) induced the formation of pppGpp and ppGpp...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02883234
更新日期：1987-01-01 00:00:00
abstract：:A total of 40 substances were tested for their inhibitory effect on the multiplication of a bacteriophage in a growing culture of Bacillus licheniformis and their influence on bacitracin production. Acriflavine was the only substance which, at a concentration of 3 microgram ml-1, completely suppressed phage multiplica...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02876597
更新日期：1978-01-01 00:00:00
abstract：:Lactobacillus bulgaricus 59 and L. casei RTS and their respective nitrosoguanidine-induced mutants were estimated for titratable and volatile acidities, production of biacetyl and acetoin and for proteolytic activity. Both the mutants showed a marked increase in proteolytic activity as compared to the parents. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02897216
更新日期：1980-01-01 00:00:00
abstract：:Addition of glucose to a resting cell suspension of the yeast Saccharomyces cerevisiae was accompanied by marked shifts of the G alpha-protein subunits from the plasma membrane to the cell interior. This process was rapid with half-times between < 10 and 20 s. The decrease of the plasma membrane pool of the Gi alpha/G...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814427
更新日期：2001-01-01 00:00:00
