Abstract:
:The plasmid R1drd-19 Km- and its derivative pON5300 were split by the PstI restriction enzyme; they differed in a 2.4 Mg/mol fragment which was present only in the pON5300. Both plasmids were present in 1-2 copies per chromosomal equivalent in E. coli JC5455 cells. The pON5300 is stable in its original mutagenized host but it segregates the enlarged region of the plasmid molecule after transformation into non-mutagenized E. coli JC5455. The possible explanation of this instability is discussed.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Navrátil O,Benada O,Braná H,Hubácek Jdoi
10.1007/BF02879554subject
Has Abstractpub_date
1983-01-01 00:00:00pages
249-52issue
4eissn
0015-5632issn
1874-9356journal_volume
28pub_type
杂志文章abstract::During cultivation in a minimal medium with glucose Alternaria tenuissima and Aspergillus vesicolor produce constitutively alpha- and beta-glucanases. Fractions of beta-1,3-glucanases exhibiting affinity for laminarin were separated by means of gel filtration chromatography. Two neutral beta-1,3-glucanases with affini...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02876394
更新日期:1980-01-01 00:00:00
abstract::The effect of subinhibitory concentrations (subMICs) of new organic ammonium salts of four homologous series of alkylammonium bromides (32 compounds) was determined with respect to the induction of lysogenic strain prophage, influence of permeability reactions in a rabbit skin test and cytotoxic changes of monolayers ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02815414
更新日期:1995-01-01 00:00:00
abstract::Kocuria spp. are widely distributed in nature. They are Gram-positive, coagulase-negative, coccoid bacteria belonging to the family Micrococcaceae, suborder Micrococcineae, order Actinomycetales, class Actinobacteria. In general, limited knowledge exists concerning the properties associated with the representants of t...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-015-0431-x
更新日期:2016-05-01 00:00:00
abstract::Phytoplankton successions under ice cover (January-March) were determined in four oligotrophic lakes (Burtjärn, Aspen, Vialamptjärn and Storkorstjärn) located in North-Eastern Sweden. The total phosphorus concentration in the lakes was less than 10 micrograms/L. Lake Burtjärn (reference lake) had a similar hydrology a...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931370
更新日期:2003-01-01 00:00:00
abstract::We isolated and identified functional groups of bacteria in the rumen of Creole goats involved in ruminal fermentation of native forage shrubs. The functional bacterial groups were evaluated by comparing the total viable, total anaerobic, cellulolytic, hemicellulolytic, and amylolytic bacterial counts in the samples t...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-012-0219-1
更新日期:2013-09-01 00:00:00
abstract::Using simultaneous fusion of protoplasts of three strains the auxotrophic K3 killer designated MH1 was prepared at a very low frequency and further employed for the transfer of the K3 killer character into a commercial wine yeast. A novel Saccharomyces Bratislava 1K3 strain with desirable technological and anti-yeast ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02814566
更新日期:1992-01-01 00:00:00
abstract::Uropathogenic Escherichia coli (UPEC) isolates contain large genomic segments, termed pathogenicity islands (PAIs), that contribute to their virulence. A total of 150 UPEC and 50 commensal E. coli isolates from outpatients were investigated for antimicrobial susceptibility and the presence of eight PAI markers. One hu...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-015-0433-8
更新日期:2016-05-01 00:00:00
abstract::The efficacy of the agents was evaluated on the basis of minimum inhibitory concentration (MIC) and time-kill curve analysis in Pseudomonas aeruginosa, Staphylococcus aureus, Acinetobacter baumannii, Mycobacterium smegmatis, and Citrobacter braakii; MIC was found to be 0.5, 4.0, 0.015625, 0.0078125, and 0.0625 mg/L in...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-009-0054-1
更新日期:2009-09-01 00:00:00
abstract::Superoxide dismutases, both cytosolic Cu, Zn-SOD encoded by SOD1 and mitochondrial Mn-SOD encoded by SOD2, serve Saccharomyces cerevisiae cells for defense against the superoxide radical but the phenotypes of sod1A and sod2delta mutant strains are different. Compared with the parent strain and the sod1delta mutant, th...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02932150
更新日期:2007-01-01 00:00:00
abstract::Cyanobacteria are one of the most successful and oldest forms of life that are present on Earth. They are prokaryotic photoautotrophic microorganisms that colonize so diverse environments as soil, seawater, and freshwater, but also stones, plants, or extreme habitats such as snow and ice as well as hot springs. This d...
journal_title:Folia microbiologica
pub_type: 杂志文章,评审
doi:10.1007/s12223-018-0626-z
更新日期:2018-09-01 00:00:00
abstract::A decrease in the content of ochratoxin A (OA) was observed in milk samples fermented by yoghurt bacteria and bifidobacteria. OA was added to the milk before fermentation at a rate of 0.05-1.5 mg/L. No residues of OA were found in samples containing 0.05 and 0.1 mg/L of OA, fermented by S. Salivarius subsp. thermophil...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02816335
更新日期:1996-01-01 00:00:00
abstract::When investigating the possible antitrichomodal effect of 41 phenylsazones, nitro- and dinitro-phenylhydrazones a significant inhibitory effect on multiplication of Trichomonas foetus could be detected in 14 derivatives; eight derivatives were ineffective, other derivatives exhibited only a slight effect. The inhibito...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02883838
更新日期:1982-01-01 00:00:00
abstract::Cryptosporidiosis belongs to the important parasitic infections with zoonotic potential and the occurrence in European countries is rare. The first cases of cryptosporidiosis caused by Cryptosporidium hominis detected in the Slovak republic were described here. Collection of examined humans consisted of five family me...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-012-0182-x
更新日期:2013-01-01 00:00:00
abstract::The diversity and structure of bacterial and actinobacteral diversity and communities were determined in a metallophytic grassland soil from an upland site in northern England. The community profiles were subjected to multivariate analyses using correspondence and cluster analyses. The total bacterial community divers...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-009-0042-5
更新日期:2009-01-01 00:00:00
abstract::Using the method of two separate UV exposures the increase of UV resistance after various induction fluences in growing and resting Escherichia coli B/r Hcr+ was followed. In resting cells, the optimum induction energy fluence was found to be 30 J/m2. In exponentially growing cells testing of induction has proved to b...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02926452
更新日期:1979-01-01 00:00:00
abstract::The cellular immune response (MIF, E-rosette formation and changes in nucleolar morphology of lymphocytes) was followed as related to age and antigenic stimulation. MIF in healthy infants increased from the 2nd to the 12th week of life as compared with the first week, probably due to BCG vaccination. The total and act...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02876400
更新日期:1980-01-01 00:00:00
abstract::Procedures to introduce point mutations, restriction sites and insert or delete DNA fragments are very important tools to study protein function. We describe here two-step PCR-based method for generating single or multiple mutations, insertions and deletions in a small region of the sequence. In the first step, a uniq...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02816213
更新日期:1999-01-01 00:00:00
abstract::Secreted aspartyl proteinase (Sap) distribution among different C. albicans isolates was determined using SAP-specific primers in polymerase chain reaction (PCR) assay. SAP1, SAP2, and SAP3 were detected in 13 of 40 (32.5%), SAP4 in 38/40 (95%), SAP5 were detected in 30/40 (75%), SAP6 in 23/40 (57.5%) of C. albicans s...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931422
更新日期:2005-01-01 00:00:00
abstract::The sorption ability of Candida utilis biomass for cadmium ions with accumulating competence of dried cells and cells in alginate was compared. After an optimization of process conditions (pH 5.5, biomass concentration 1 g/L and c0 50 mg/L), the cadmium sorption capacity of dried yeast biomass was perceptibly higher t...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931807
更新日期:2006-01-01 00:00:00
abstract::Coxiella burnetii (C. burnetii), an intracellular zoonotic bacterium causing Q fever, occurs widely in cattle herds. After invasion of the pregnant uterus and initial localization in the placenta, active C. burnetii infections may spread to the fetus hematogenously or by the amniotic-oral route and thus may cause abor...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-017-0553-4
更新日期:2018-03-01 00:00:00
abstract::Biofilms represent mixed communities present in a diverse range of environments; however, their utility as inoculants is less investigated. Our investigation was aimed towards in vitro development of biofilms using fungal mycelia (Trichoderma viride) as matrices and nitrogen-fixing and P-solubilizing bacteria as partn...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-012-0154-1
更新日期:2012-09-01 00:00:00
abstract::Cryptococcus neoformans is encapsulated yeast that causes cryptococcosis. The cryptococcal meningitis may cause neuropsychiatric symptoms. Here, we evaluated the in vitro activity of amphotericin B (AMB), chlorpromazine (CLOR), and sertraline (SERT) alone or in combination against clinical isolates of C. neoformans co...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-016-0449-8
更新日期:2016-09-01 00:00:00
abstract::Strains of Rhodospordium torloides produced two peptides into the culture filtrate. Peptide I contained 6 amino acids and peptide II contained 5 amino acids. The peptides however, are, not homologous to the alpha-factor, and become assimilated at a later phase of growth. ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02876614
更新日期:1980-01-01 00:00:00
abstract::A strain of Aspergillus giganteus cultivated in a medium with xylan produced two xylanases (xylanase I and II) which were purified to homogeneity. Their molar mass, estimated by SDS-PAGE, were 21 and 24 kDa, respectively. Both enzymes are glycoproteins with 50 degrees C temperature optimum; optimum pH was 6.0-6.5 for ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931639
更新日期:2004-01-01 00:00:00
abstract::A method is presented for the successful decontamination of sparingly soluble pteridine derivatives by microwave irradiation. The method is nondestructive, rapid and effective in eliminating contamination. ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02814514
更新日期:1991-01-01 00:00:00
abstract::The addition of a nitrogen (nitrate) and carbon sources (acetate, citrate and fructose) and phosphate deficiency (nitrate medium deficient in phosphate) under unaerated conditions induced akinete differentiation in Anabaena torulosa. Aerated cultures of this organism in these nutrients did not differentiate akinetes. ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02817617
更新日期:2000-01-01 00:00:00
abstract::Cord and newborn sera were found to possess a significantly decreased in vitro bactericidal activity to Escherichia coli as compared to sera of adult individuals. Hyperbilirubinaemia in neonates suffering from both Rh and ABO incompatibility results in a further significant impairment of the bactericidal activity of t...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02927298
更新日期:1979-01-01 00:00:00
abstract::We report the complete nucleotide sequence and characterization of a small cryptic plasmid of Moraxella macacae 0408225, a newly described bacterial species within the family Moraxellaceae and a causative agent of epistaxis in macaques. The complete nucleotide sequence of the plasmid pMoma1 was determined and found to...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-014-0364-9
更新日期:2015-05-01 00:00:00
abstract::The biosynthetic gene cluster of porothramycin, a sequence-selective DNA alkylating compound, was identified in the genome of producing strain Streptomyces albus subsp. albus (ATCC 39897) and sequentially characterized. A 39.7 kb long DNA region contains 27 putative genes, 18 of them revealing high similarity with hom...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-014-0339-x
更新日期:2014-11-01 00:00:00
abstract::Using both monoclonal and polyclonal antibodies against mammalian plectin (multifunctional protein cross-linking cytoskeletal structures, mainly intermediate filaments, in mammalian cells), several putative isoforms of plectin-like proteins were found in protein extracts from the green alga Chlamydomonas eugametos (Vo...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02818794
更新日期:2002-01-01 00:00:00