Abstract：
:Cryptosporidiosis belongs to the important parasitic infections with zoonotic potential and the occurrence in European countries is rare. The first cases of cryptosporidiosis caused by Cryptosporidium hominis detected in the Slovak republic were described here. Collection of examined humans consisted of five family members. Faecal specimens were examined by formalin sedimentation, by the Sheather's sugar flotation and by immunochromatography and visualised by the Ziehl-Neelsen acid fast stain. A fragment of the Cryptosporidium small subunit ribosomal RNA gene was amplified by nested polymerase chain reaction and species was determined by restriction fragment length polymorphism analysis with the endonucleases SspI and VspI. C. hominis was found in faeces of two immunocompetent siblings (a 7-year-old boy and a 2-year-old girl). The symptoms occurred only in the boy as gastrointestinal disorders lasting 5 days, and manifested by abdominal pain, an elevated body temperature (37.2 °C), mild diarrhoea, accompanied by lassitude, depression and anorexia. Ultrasonic scan revealed enlarged spleen and mezenteric lymph nodes. Microscopic examination of the stool sample revealed numerous Cryptosporidium oocysts. The DNA typing identified C. hominis subtype IbA10G2. Cryptosporidium was also detected in the boy's sister without any complications and symptoms. Their father, mother and grandmother were parasitologically negative. The source of infection remained unknown. Human cases in present study reflect necessity of systematic attention on intestinal parasites diagnostic inclusive of cryptosporidia.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Ondriska F,Vrabcová I,Brinďáková S,Kváč M,Ditrich O,Boldiš V,Bastlová Mdoi
10.1007/s12223-012-0182-xsubject
Has Abstractpub_date
2013-01-01 00:00:00pages
69-73issue
1eissn
0015-5632issn
1874-9356journal_volume
58pub_type
杂志文章abstract：:Hemolytic factor production by pathogenic Candida species is considered an important attribute in promoting survival within the mammal host through the ability to assimilate iron from the hemoglobin-heme group. Hemolytic capability has been evaluated for Candida species based on hemolysis zones on plate assay, analysi...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/s12223-018-0584-5
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abstract：:An extracellular acid phosphatase isolated from the culture of a wild strain Aspergillus niger, producing the dephosphorylating 3-phytase, was obtained in a homogeneous form by sequential application of ultrafiltration through PS 50 membrane, gel filtration on Sephadex G-100 and ion exchange chromatography on DEAE-Sep...
journal_title：Folia microbiologica
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doi：10.1007/BF02931618
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journal_title：Folia microbiologica
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doi：10.1007/BF02876433
更新日期：1978-01-01 00:00:00
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journal_title：Folia microbiologica
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doi：10.1007/BF02931569
更新日期：2005-01-01 00:00:00
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journal_title：Folia microbiologica
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更新日期：2002-01-01 00:00:00
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journal_title：Folia microbiologica
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更新日期：1980-01-01 00:00:00
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journal_title：Folia microbiologica
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doi：10.1007/s12223-019-00704-4
更新日期：2019-11-01 00:00:00
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journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02817680
更新日期：2002-01-01 00:00:00
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journal_title：Folia microbiologica
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doi：10.1007/BF02927304
更新日期：1979-01-01 00:00:00
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journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-019-00752-w
更新日期：2020-06-01 00:00:00
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journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931330
更新日期：2003-01-01 00:00:00
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journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02927365
更新日期：1981-01-01 00:00:00
abstract：:Fibrobacter succinogenes is a major cellulolytic species in the rumen. On the basis of molecular data, this species can be classified into four phylogenetic groups. Recently gathered ecological and physiological data have revealed the importance of this species, particularly phylogenetic group 1, in rumen fiber digest...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/s12223-008-0024-z
更新日期：2008-01-01 00:00:00
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journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931628
更新日期：2006-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s12223-017-0573-0
更新日期：2018-05-01 00:00:00
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journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-010-0076-8
更新日期：2010-09-01 00:00:00
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journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-010-0029-2
更新日期：2010-05-01 00:00:00
abstract：:Rumen ciliate protozoa intensively engulf bacteria. However, their ability to utilize murein which is the main polysaccharide of bacterial cell wall has hardly been recognized. The present study concerns the ability of the rumen protozoa Diploplastron affine to digest and ferment murein. The ciliates were isolated fro...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-010-0047-0
更新日期：2010-07-01 00:00:00
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journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814494
更新日期：1991-01-01 00:00:00
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journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02927178
更新日期：1979-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/BF02876611
更新日期：1980-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：1980-01-01 00:00:00
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journal_title：Folia microbiologica
pub_type： 杂志文章
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更新日期：2002-01-01 00:00:00
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doi：10.1007/BF02877396
更新日期：1982-01-01 00:00:00
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journal_title：Folia microbiologica
pub_type： 杂志文章
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更新日期：2003-01-01 00:00:00
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journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02926452
更新日期：1979-01-01 00:00:00
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journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02879491
更新日期：1983-01-01 00:00:00
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journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931639
更新日期：2004-01-01 00:00:00
abstract：:We constructed a SmaI genomic library of Cellulomonas biazotea DNA in E. coli and in the S. cerevisiae shuttle vector, YEP 24. Three clone were identified that conferred the ability for E. coli or S. cerevisiae transformants to produce carboxymethylcellulase (CMCase). Cells transformed with these clones were compared ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814307
更新日期：1994-01-01 00:00:00
