Abstract:
:Fifty Escherichia coli strains isolated from stool samples of 51 healthy children, 143 strains isolated from stool samples of 327 children with diarrhea and 24 strains isolated from stool samples of 21 children with suspected hemolytic uremic syndrome were examined for the presence of Shiga toxin-producing E. coli virulence factors (shiga toxin 1 and 2, intimin and enterohemolysin) and their genes. Vero-cell assay and latex agglutination were used for detection of Shiga toxin 1 and 2, TSB agar with washed erythrocytes was used for detection of enterohemolysin; genes encoding shiga toxin 1 and 2, intimin and enterohemolysin were detected using multiplex PCR. The presence of E. coli strains harboring genes encoding shiga toxin 1 and 2 (12 strains), intimin (34 strains) and enterohemolysin (12 strains) was demonstrated.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Liptáková A,Siegfried L,Podracká L,Sabol M,Sehnálková H,Bogyiová E,Rosocha J,Kmetová M,Kerestesová H,Kotulová Ddoi
10.1007/BF02817680keywords:
subject
Has Abstractpub_date
2002-01-01 00:00:00pages
185-8issue
2eissn
0015-5632issn
1874-9356journal_volume
47pub_type
杂志文章abstract::Frequency of heterocytes and nitrogenase activity (NA) under light and dark cultivation conditions was determined in 12 cyanobacterial strains isolated from various soil habitats. In spite of a high variability, significant differences in NA among the strains were found in response of light and dark cultivation. Relat...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931606
更新日期:2004-01-01 00:00:00
abstract::The effect of foliar application of 2% urea and 0.6% benomyl on changes in colonization of the rhizosphere by microorganisms and of roots by the fungus Gaeumannomyces graminis (Sacc.) Arx et Olivier var. tritici Walker was followed in vegetation glass-house experiments. Treatment with a urea solution resulted in incre...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02897213
更新日期:1980-01-01 00:00:00
abstract::Eleven purified lectins were compared in their IgE-precipitating, mast-cell-degranulating and histamine-releasing activity and in eliciting skin allergic reactions in atopic or normal people. A significantly positive relationship could be proved between their IgE-precipitating activity and degranulation of IgE-sensiti...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02923516
更新日期:1985-01-01 00:00:00
abstract::Enzyme synthesis of tryptophan from indole, pyruvate and ammonium salts was studied using Escherichia coli cells exhibiting a significant tryptophanase activity. In addition to the effect of cultivation medium composition and cultivation conditions, factors affecting the course of the conversion were investigated. Pro...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02820485
更新日期:1990-01-01 00:00:00
abstract::Phytoplankton successions under ice cover (January-March) were determined in four oligotrophic lakes (Burtjärn, Aspen, Vialamptjärn and Storkorstjärn) located in North-Eastern Sweden. The total phosphorus concentration in the lakes was less than 10 micrograms/L. Lake Burtjärn (reference lake) had a similar hydrology a...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931370
更新日期:2003-01-01 00:00:00
abstract::The microbial fuel cells (MFCs) are recognized to be highly effective for the biodegradation of phenol. For isolating the phenol-degrading bacteria, the sample containing 500 mg/L phenol was collected from the MFCs. The strain (WL027) was identified basing on the 16S rRNA gene analysis and phylogenetic analysis as Bac...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-017-0536-5
更新日期:2018-01-01 00:00:00
abstract::Vinasse, a recalcitrant waste of the ethanol industry was employed for the production of polyhydroxyalkanoate (PHA) by the extremely halophilic archaeon, Haloarcula marismortui in shake flasks. The PHA was recovered by osmotic lysis of the cells and subsequent purification by sodium hypochlorite and organic solvents. ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-011-0092-3
更新日期:2012-01-01 00:00:00
abstract::Staphylococcus aureus is a leading cause of bloodstream infections (BSI) and diseases that may be caused by hematogenous spread. The staphylococcal adhesin, for which the association with the infections emerging as a complication of septicemia has been well documented, is a bone sialoprotein-binding protein (Bbp). The...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-014-0321-7
更新日期:2014-11-01 00:00:00
abstract::Strains ofRhodotorula sp.,Candida spp. andLangermania sp. cultivated on polyunsaturated oil preferentially incorporated more unsaturated fatty acids. These fatty acids were used mainly for growth needs whereas the saturated ones accumulated in the microbial cell. The cellular oil and the remaining oil in the culture h...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02898718
更新日期:1997-04-01 00:00:00
abstract::Pullulanase of Klebsiella oxytoca is one of a wide variety of extracellular proteins that are secreted by Gram-negative bacteria by the complex main terminal branch (MTB) of the general secretory pathway. The roles of some of the 14 components of the MTB are now becoming clear. In this review it is proposed that most ...
journal_title:Folia microbiologica
pub_type: 杂志文章,评审
doi:10.1007/BF02818976
更新日期:1997-01-01 00:00:00
abstract::Fungal cells of Aureobasidium pullulans ATCC 201253 were immobilized by entrapment in chitosan beads, and the immobilized cells were investigated for their ability to produce the polysaccharide pullulan using batch fermentation. The 1% chitosan-entrapped fungal cells were capable of producing pullulan for two cycles o...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-011-0048-7
更新日期:2011-07-01 00:00:00
abstract::Sixty four biopsy specimens of oral Candida leukoplakias were examined. Histological sections were stained with periodic acid-Schiff (PAS) reagent and by Grocott's silver method. C. albicans isolates were cultivated from all the patients but fungal hyphae were proved histologically only in 23 of them. PAS-positive and...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02814437
更新日期:2001-01-01 00:00:00
abstract::Virulence factors were compared in 15 Aeromonas spp. isolated from faeces of patients with Aeromonas-associated gastroenteritis and in 81 strains isolated from food. Strains from food did not show differences in the distribution of virulence factors when compared with strains isolated from faeces. However, 88.8% of Ae...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02814323
更新日期:1994-01-01 00:00:00
abstract::The highest level of secreted brefeldin A was present in glucose-grown cultures, intermediate levels in glucose-fructose, and xylose cultures and low levels in fructose- and galactose-grown cultures of Curvularia lunata. The biomass decreased from glucose, fructose, xylose, glucose-fructose to galactose cultures. Bref...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02876993
更新日期:1977-01-01 00:00:00
abstract::The bacterial strain KDF8 capable of growth in the presence of diclofenac and codeine analgesics was obtained after chemical mutagenesis of nature isolates from polluted soils. The strain KDF8 was identified as Raoultella sp. based on its morphology, biochemical properties, and 16S rRNA gene sequence. It was deposited...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-017-0563-2
更新日期:2018-05-01 00:00:00
abstract::The color of light (white, red, blue, and green) had a significant effect on the growth and reproductive processes (both in the nucleocytoplasmic and chloroplast compartment of the cells) in synchronous cultures of Scenedesmus obliquus. This effect decreased in the order red > white > blue > green. In the same order, ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931828
更新日期:2006-01-01 00:00:00
abstract::The present paper reports a modified method for isolation of lysostaphin--a bacteriolytic agent with specific affinity for staphylococcal cell wall. The proposed purification scheme includes three steps. The first procedure is ultrafiltration through a membrane filter giving a yield of 75.6%. The result of ultrafiltra...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02814386
更新日期:1993-01-01 00:00:00
abstract::Levels of phenotypic and genotypic diversity among seven Megasphaera elsdenii strains recovered from rumen contents of cattle, sheep and lambs were determined by a combination of antibiotic-resistance analysis and PCR fingerprint techniques targeted both to the ribosomal RNA operon (ARDRA, RISA) and the whole genome (...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931818
更新日期:2006-01-01 00:00:00
abstract::Activity of purified alanylaminopeptidase of Pseudomonas sp. measured in the presence of the alanine derivative of 2-naphthoic acid (NA-Ala) is inhibited by 1,10-phenanthroline, EDTA, bestatin and amastatin; this finding supports the conclusion that this enzyme is a metallo-aminopeptidase. A decrease of its activity i...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02817643
更新日期:2002-01-01 00:00:00
abstract::Vancomycin is often used in orthopedic surgery as a local prophylaxis of bacterial infection. The aim of this work was to compare the release of vancomycin and its biologically inactive crystalline degradation products (CDP-1) during in vitro experiments from different types of local antibiotic delivery systems (bone ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-019-00752-w
更新日期:2020-06-01 00:00:00
abstract::Contemporary aspects of cerebrospinal fluid analysis are discussed, including the relationship to neuro-infective, autoimmune and other neurological diseases. The actual state of cerebrospinal fluid microbiological and cytological investigation and analysis of cerebrospinal fluid protein fractions are described in det...
journal_title:Folia microbiologica
pub_type: 杂志文章,评审
doi:10.1007/BF02931522
更新日期:2003-01-01 00:00:00
abstract::The sorption ability of Candida utilis biomass for cadmium ions with accumulating competence of dried cells and cells in alginate was compared. After an optimization of process conditions (pH 5.5, biomass concentration 1 g/L and c0 50 mg/L), the cadmium sorption capacity of dried yeast biomass was perceptibly higher t...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931807
更新日期:2006-01-01 00:00:00
abstract::Hydroxy, n-saturated, branched, dioic, and unsaturated fatty acids in six freshwater wild cyanobacteria (Chroococcus minutus, Lyngbya ceylanica, Merismopedia glauca, Nodularia sphaerocarpa, Nostoc linckia, and Synechococcus aeruginosus) collected from different lakes and springs of Israel have been identified by gas c...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931279
更新日期:2003-01-01 00:00:00
abstract::Treatment of Helicobacter pylori cells with several chaotropic agents resulted in different degrees of inhibition in the binding of the bacteria to hemin and Congo-red dye. Polyanions also yielded a >50% inhibitory effect. Furthermore, hydrophobic interaction chromatography was used to determine the relative surface h...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-011-0043-z
更新日期:2011-05-01 00:00:00
abstract::Two Bacillus cereus feather-degrading strains (23/1 and 6/2) were transformed using a recombinant plasmid p5.2 carrying the alkaline proteinase gene (aprE). A high level of the aprE gene expression was observed when the recombinant strains were grown on sporulation medium. The expression of the aprE gene proceeded dur...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-010-0004-y
更新日期:2010-01-01 00:00:00
abstract::Mineral nutrition of crop plants is one of the major challenges faced by modern agriculture, particularly in arid and semi-arid regions. In alkaline calcareous soils, the availability of phosphorus and zinc is critically less due to their fixation and precipitation as complexes. Farmers use fertilizers to fulfill crop...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-020-00831-3
更新日期:2020-10-24 00:00:00
abstract::Among 18 tested strains of Claviceps spp., 7 produced significant amounts of exocellular polysaccharide (EPS). The maximum production of EPS was found in fermentation broth of Claviceps viridis. The kinetics of growth, substrate consumption, and EPS production in the batch, aerobic, submerged culture of this fungus we...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02993470
更新日期:2003-01-01 00:00:00
abstract::Rumen ciliate protozoa intensively engulf bacteria. However, their ability to utilize murein which is the main polysaccharide of bacterial cell wall has hardly been recognized. The present study concerns the ability of the rumen protozoa Diploplastron affine to digest and ferment murein. The ciliates were isolated fro...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-010-0047-0
更新日期:2010-07-01 00:00:00
abstract::The pathogenesis of bacillary dysentery can be studied at different levels of integration of the cellular components that constitute the colonic mucosal barrier. We considered the interaction of Shigella flexneri in three experimental systems that provide complementary information and a scheme of events occurring in h...
journal_title:Folia microbiologica
pub_type: 杂志文章,评审
doi:10.1007/BF02818608
更新日期:1998-01-01 00:00:00
abstract::This study was undertaken to develop a new Gram-staining machine controlled by a micro-controller and to investigate the quality of slides that were stained in the machine. The machine was designed and produced by the authors. It uses standard 220 V AC. Staining, washing, and drying periods are controlled by a timer b...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02818557
更新日期:1999-01-01 00:00:00