Abstract：
:Fungal cells of Aureobasidium pullulans ATCC 201253 were immobilized by entrapment in chitosan beads, and the immobilized cells were investigated for their ability to produce the polysaccharide pullulan using batch fermentation. The 1% chitosan-entrapped fungal cells were capable of producing pullulan for two cycles of 168 h using corn syrup as a carbon source. Pullulan production by the immobilized cells increased by 1.6-fold during the second production cycle (5.0 g/l) relative to the first production cycle (3.1 g/l) with the difference in production being statistically significant after 168 h. The productivity of the immobilized cells increased during the second production cycle while its pullulan content decreased. The level of cell leakage from the support remained unchanged for both production cycles.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
West TPdoi
10.1007/s12223-011-0048-7subject
Has Abstractpub_date
2011-07-01 00:00:00pages
335-8issue
4eissn
0015-5632issn
1874-9356journal_volume
56pub_type
杂志文章abstract：:Competitive PCR method was developed for the detection and enumeration of Butyrivibrio fibrisolvens. Sequences of 16S rDNA were obtained from our isolates (serving as a source of data for primer design) and were distinguished into nine different groups of butyrivibria. Specific primers for two distinct groups were des...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02825888
更新日期：2001-01-01 00:00:00
abstract：:A naturally deficient thiamine and methionine requiring strain of Bacillus coagulans (Ms 5) accumulates lysine in medium only when exogenous pyridoxine (optimal concentration, 0.1 mu g/ml) are supplied. Threonine exerts an inhibitory effect at higher concentrations but pyridoxine does not. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02877085
更新日期：1975-01-01 00:00:00
abstract：:3-(1,1-Dioxadibenzothien-4-oyl)acrylic acid (1) was condensed with compounds containing active methylene groups under Michael reaction conditions to furnish the Michael adducts (lactones 2a-c, lactams 3a-c, ketones 4a,b). The behavior of these adducts toward the action of hydrazine hydrate were investigated. The compo...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02817670
更新日期：2002-01-01 00:00:00
abstract：:Virus-like particles (VLPs) were isolated from the yeast Wickerhamia fluorescens strain CCY61-1-1. The VLPs are approximately 42 nm in diameter and contain only one species of dsRNA molecule. The apparent length of the dsRNA determined by native agarose gel electrophoresis was 4.6 kbp. Analysis of protein content of t...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814620
更新日期：1996-01-01 00:00:00
abstract：:Escherichia coli strain HB101 harboring an expression plasmid bearing calf prochymosin gene under the control of the tac promoter was grown in the presence of IPTG with or without novobiocin at 28 and 40 degrees C, respectively. The differential rates of synthesis of prochymosin inclusions, and, for comparison, of bet...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02815545
更新日期：1998-01-01 00:00:00
abstract：:Superoxide dismutases, both cytosolic Cu, Zn-SOD encoded by SOD1 and mitochondrial Mn-SOD encoded by SOD2, serve Saccharomyces cerevisiae cells for defense against the superoxide radical but the phenotypes of sod1A and sod2delta mutant strains are different. Compared with the parent strain and the sod1delta mutant, th...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02932150
更新日期：2007-01-01 00:00:00
abstract：:A method is presented for the successful decontamination of sparingly soluble pteridine derivatives by microwave irradiation. The method is nondestructive, rapid and effective in eliminating contamination. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814514
更新日期：1991-01-01 00:00:00
abstract：:The biosynthetic gene cluster of porothramycin, a sequence-selective DNA alkylating compound, was identified in the genome of producing strain Streptomyces albus subsp. albus (ATCC 39897) and sequentially characterized. A 39.7 kb long DNA region contains 27 putative genes, 18 of them revealing high similarity with hom...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-014-0339-x
更新日期：2014-11-01 00:00:00
abstract：:The synthesis of exocellular proteinase decreases with increasing concentration of amino acids in the growth medium. After removal of amino acids the enzyme synthesis is gradually restored to normal values. The presence of inhibitors of transcription (actinomycin D) or translation (chloramphenicol) blocks the restorat...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02875957
更新日期：1984-01-01 00:00:00
abstract：:The prevalence of extended-spectrum beta-lactamases (ESBL) was determined among isolates of Escherichia coli (n = 63) isolated from hospitalized (43) and healthy (20) children. Ten isolates (21%) were ESBL-positive for two screening tests, the double disk-synergy test and the Oxoid Combination Disk method. One ESBL-po...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02930963
更新日期：2003-01-01 00:00:00
abstract：:The efficacy of the agents was evaluated on the basis of minimum inhibitory concentration (MIC) and time-kill curve analysis in Pseudomonas aeruginosa, Staphylococcus aureus, Acinetobacter baumannii, Mycobacterium smegmatis, and Citrobacter braakii; MIC was found to be 0.5, 4.0, 0.015625, 0.0078125, and 0.0625 mg/L in...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-009-0054-1
更新日期：2009-09-01 00:00:00
abstract：:Resistance determinants for HgCl2 and CdCl2 were eliminated along with a number of antibiotic resistance factors from Acinetobacter lwoffi and Micrococcus sp. at 44 degrees C. These organisms were orginally resistant to HgCl2, merbromin, CdCl2, Pb(NO3)2, benzylpenicillin, erythromycin, carbenicillin, tetracycline and ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02925907
更新日期：1988-01-01 00:00:00
abstract：:The present study was conducted to determine the gene responsible for beta-glucosidase (BGL) production and to generate a full-length complementary DNA (cDNA) of one of the putative BGL genes, which showed a significant expression level when Schizophyllum commune KUC9397 was grown in optimized medium. The relative exp...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-016-0484-5
更新日期：2017-05-01 00:00:00
abstract：:The biochemical basis of a defective bioenergetic system was attempted to be determined in N,N'-dicyclohexylcarbodiimide (DCCD)-resistant mutant of Methanothermobacter thermautotrophicus. Components participating in the maintenance of methanoarchaeal membrane structure and function, such as the composition of the mixt...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-008-0034-x
更新日期：2008-01-01 00:00:00
abstract：:The aim of this study was to determine the prevalence of Listeria sp. and Listeria monocytogenes in soil samples with reference to type of fertilizers (natural and artificial) and distance from places intensively exploited by men, as well as to determine the relationship between the presence of L. monocytogenes in the...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-013-0260-8
更新日期：2014-01-01 00:00:00
abstract：:Three types of heterogeneous preparations and four types of preparations of polysaccharide nature were obtained in studies aimed at the isolation of active compounds from Aspergillus flavus conidia bearing their biological stimulatory activity. Extraction with trichloroacetic acid at 0 degrees C yielded a preparation ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02927425
更新日期：1981-01-01 00:00:00
abstract：:Using an in vitro cytotoxicity assay (51Cr-release), it was shown that bilirubin exerts a cytotoxic effect on both adult and newborn human lymphocytes after a 1-d incubation of cells with bilirubin. The effect of bilirubin on human granulocytes was less pronounced; the association of the cytotoxic effect with a functi...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02923520
更新日期：1985-01-01 00:00:00
abstract：:Sporulation was repressed in the parent strain by various carbon sources whereas glucose-resistant mutants were resistant to them but not to glycerol 2-phosphate. Both mycobacillin and dipicolinic acid synthesis were repressed in the parent by some of the compounds tested, viz. glucose, pyruvate and glycerol 2-phospha...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02928000
更新日期：1986-01-01 00:00:00
abstract：:Three indigenous plasmids designated pRK1, pRK2 and pRK3 were identified among producers of penicillin G acylase (PGA) derived from the strain Escherichia coli W ATCC 9637. Their size and copy number (CN) in E. coli W were determined (kb; CN): pRK1 (80; 3.4), pRK2 (5.1; 71), and pRK3 (4.8; 13.7). Strain E. coli RE2 ha...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02818544
更新日期：1999-01-01 00:00:00
abstract：:Ecosystems worldwide are exposed to pollutants connected to the industrial production of pharmaceuticals. The objective of this study was to study the composition and characteristics of the soil microbial communities that had been exposed to long-term selection pressure caused by the industrial production of penicilli...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-020-00819-z
更新日期：2020-12-01 00:00:00
abstract：:Many serious diseases caused by Staphylococcus aureus appear to be associated with biofilms. Therefore, we investigated the biofilm-forming ability of the methicillin-resistant S. aureus (MRSA) isolates collected from hospitalized patients. As many as 96 % strains had the ability to form biofilm in vitro. The majority...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-012-0175-9
更新日期：2013-01-01 00:00:00
abstract：:A proteus strain inhibited mycelial growth of Fusarium oxysporum in vitro. Seed bacterization showed significant plant growth promotion and Fusarium-wilt suppression activity of Phaseolus mungo in a gnotobiotic system. The culture filtrate of this strain exhibited three prominent bands in UV-VIS spectra between 300 an...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02818723
更新日期：2000-01-01 00:00:00
abstract：:The present paper reports a modified method for isolation of lysostaphin--a bacteriolytic agent with specific affinity for staphylococcal cell wall. The proposed purification scheme includes three steps. The first procedure is ultrafiltration through a membrane filter giving a yield of 75.6%. The result of ultrafiltra...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814386
更新日期：1993-01-01 00:00:00
abstract：:Anaerobic ammonium-oxidizing (anammox) bacteria play an essential part in nitrogen removal in constructed wetlands. The objective of the present study was to explore the spatiotemporal dynamics of anammox bacterial communities and the associated factors in a full-scale constructed wetland for the treatment of polluted...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-019-00726-y
更新日期：2020-04-01 00:00:00
abstract：:Altered cell signaling is the molecular basis for cell proliferation occurring in association with several gamma herpesvirus infections. Three gamma herpesviruses, namely EBV/HHV-4, KSHV/HHV-8 and the MHV-68 (and/or MHV-72) and their unusual cell-pirated gene products are discussed in this respect. The EBV, KSHV as we...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/BF02931360
更新日期：2003-01-01 00:00:00
abstract：:Several amino acids affected the amount of bacitracin produced by Bacillus licheniformis ATCC 10716 and the high-yielding mutant B. licheniformis AL. The possibility of an amino acid control mechanism for bacitracin biosynthesis is discussed. The two strains showed a different response to the addition of some amino ac...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02926454
更新日期：1979-01-01 00:00:00
abstract：:This study was undertaken to develop a new Gram-staining machine controlled by a micro-controller and to investigate the quality of slides that were stained in the machine. The machine was designed and produced by the authors. It uses standard 220 V AC. Staining, washing, and drying periods are controlled by a timer b...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02818557
更新日期：1999-01-01 00:00:00
abstract：:The effect of subinhibitory concentrations (subMICs) of new organic ammonium salts of four homologous series of alkylammonium bromides (32 compounds) was determined with respect to the induction of lysogenic strain prophage, influence of permeability reactions in a rabbit skin test and cytotoxic changes of monolayers ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02815414
更新日期：1995-01-01 00:00:00
abstract：:Purification of streptococcal extracellular NAD+ nucleosidase is associated with changes of kinetic properties. A high-molecular weight component is required for a full activity of the enzyme. The component is not produced by bacteria and is present in the Todd-Hewitt cultivation medium, the beef-heart extract serving...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02876396
更新日期：1980-01-01 00:00:00
abstract：:Fourteen of 200 Lactobacillus isolates from African fermented foods, viz. 'wara', 'kenkey', 'ugba', 'ogi', 'kunuzarki', 'fufu' and 'iru' were found to produce bacteriocins against L. plantarum and only three bacteriocinogenic isolates inhibited some of the food pathogens. Plasmid analysis of the 14 bacteriocin-produci...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814645
更新日期：1994-01-01 00:00:00
