Abstract：
:The present study was conducted to determine the gene responsible for beta-glucosidase (BGL) production and to generate a full-length complementary DNA (cDNA) of one of the putative BGL genes, which showed a significant expression level when Schizophyllum commune KUC9397 was grown in optimized medium. The relative expression levels of seven genes encoding BGL of S. commune KUC9397 were determined with real-time quantitative reverse transcription PCR in cellulose-containing optimized medium (OM) compared to glucose-containing basal medium (BM). The most abundant transcript was bgl3a in OM. The transcript number of the bgl3a increased more than 57.60-fold when S. commune KUC9397 was grown on cellulose-containing OM compared to that on glucose-containing BM. The bgl3a was identified, and a deduced amino acid sequence of bgl3a shared homology (97%) with GH3 BGL of S. commune H4-8. This is the first report showing the transcription levels of genes encoding BGL and identification of full-length cDNA of glycoside hydrolase 3 (GH3) BGL from S. commune. Furthermore, this study is one of the steps for consolidated bioprocessing of lignocellulosic biomass to bioethanol.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Lee YM,Lee H,Heo YM,Lee H,Hong JH,Kim JJdoi
10.1007/s12223-016-0484-5subject
Has Abstractpub_date
2017-05-01 00:00:00pages
191-196issue
3eissn
0015-5632issn
1874-9356pii
10.1007/s12223-016-0484-5journal_volume
62pub_type
杂志文章abstract：:The addition of a nitrogen (nitrate) and carbon sources (acetate, citrate and fructose) and phosphate deficiency (nitrate medium deficient in phosphate) under unaerated conditions induced akinete differentiation in Anabaena torulosa. Aerated cultures of this organism in these nutrients did not differentiate akinetes. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02817617
更新日期：2000-01-01 00:00:00
abstract：:Virus-like particles (VLPs) were isolated from the yeast Wickerhamia fluorescens strain CCY61-1-1. The VLPs are approximately 42 nm in diameter and contain only one species of dsRNA molecule. The apparent length of the dsRNA determined by native agarose gel electrophoresis was 4.6 kbp. Analysis of protein content of t...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814620
更新日期：1996-01-01 00:00:00
abstract：:Acmycin, a new polyene antibiotic, was fungistatic at low and fungicidal at high concentrations. It produced malformations in conidia of Helminthosporium oryzae and Curvularia lunata and caused leakage of yeast cells. Acmycin was fairly nontoxic to germinating seeds. The intravenous LD50 was 300-400 and intraperitonea...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02879559
更新日期：1983-01-01 00:00:00
abstract：:Plenty of metagenomic studies have suggested possible associations between microbiome composition and colorectal cancer (CRC). However, these techniques are not economic enough for routine use so far. Therefore, we explored the possibility to detect species associated with colorectal cancer by conventional culture fro...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-020-00807-3
更新日期：2020-12-01 00:00:00
abstract：:Strains ofRhodotorula sp.,Candida spp. andLangermania sp. cultivated on polyunsaturated oil preferentially incorporated more unsaturated fatty acids. These fatty acids were used mainly for growth needs whereas the saturated ones accumulated in the microbial cell. The cellular oil and the remaining oil in the culture h...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02898718
更新日期：1997-04-01 00:00:00
abstract：:Ten thermophilic Gram-positive bacteria were isolated from various soils of Saudi Arabia. The strains are spore-forming rods belonging to the species Bacillus stearothermophilus. The cells are motile, strictly aerobic, catalase and oxidase positive. The sporangia appear to be swollen and their position varies from ter...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02927328
更新日期：1981-01-01 00:00:00
abstract：:The effects of pH on denitrifying enzyme activity (DEA) and on the ratio of the denitrification products, N(2)O and N(2), were determined in three pasture soils differing in cattle impact. The linkage between intrinsic differences in the denitrifying communities and pH effects on relative N(2)O production was also ass...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-011-0045-x
更新日期：2011-05-01 00:00:00
abstract：:This is the first report describing precipitation of bacteriophage particles with zinc chloride as a method of choice to isolate infectious lytic bacteriophages against Pectobacterium spp. and Dickeya spp. from environmental samples. The isolated bacteriophages are ready to use to study various (ecological) aspects of...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-015-0411-1
更新日期：2016-01-01 00:00:00
abstract：:The ability of six bifidobacterial strains (3 of human origin and 3 isolates from fermented milk products) to utilize glucose, lactose, melezitose, sucrose, raffinose, and stachyose was determined. Dairy-related bifidobacterial strains were identified as Bifidobacterium animalis (2 strains) or as B. pseudolongum (1 st...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02818784
更新日期：2002-01-01 00:00:00
abstract：:Viridans group streptococci bloodstream infections (VGS BSI) remain a significant cause of mortality and morbidity in patients with severe neutropenia. The goal of our study was to evaluate clinical course and microbiological susceptibility of VGS BSI at our center. Retrospective analysis of all microbiologically docu...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-017-0542-7
更新日期：2018-03-01 00:00:00
abstract：:In the present study, three Arcanobacterium pluranimalium strains isolated from bovine milk samples of three cows of three farms (two cows with subclinical mastitis) could successfully be identified by phenotypical investigations, by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-T...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-016-0450-2
更新日期：2016-09-01 00:00:00
abstract：:Biodegradation of two amphiphilic "soft" antimicrobially active derivatives of lauric (dodecanoic) acid, a quaternary ammonium salt and an amine oxide bearing an amide or ester group, was followed using microorganisms from activated sludge. Primary biodegradation was determined by ion-selective electrodes, total biode...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814548
更新日期：1993-01-01 00:00:00
abstract：:Mineral nutrition of crop plants is one of the major challenges faced by modern agriculture, particularly in arid and semi-arid regions. In alkaline calcareous soils, the availability of phosphorus and zinc is critically less due to their fixation and precipitation as complexes. Farmers use fertilizers to fulfill crop...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-020-00831-3
更新日期：2020-10-24 00:00:00
abstract：:Using simultaneous fusion of protoplasts of three strains the auxotrophic K3 killer designated MH1 was prepared at a very low frequency and further employed for the transfer of the K3 killer character into a commercial wine yeast. A novel Saccharomyces Bratislava 1K3 strain with desirable technological and anti-yeast ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814566
更新日期：1992-01-01 00:00:00
abstract：:Strains of genus Citrobacter (152 isolates from 1950 to 1988 deposited in the Czech National Collection of Type Cultures, Prague) were re-classified using biological and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) methods. One-hundred thirty-six strains (ca. 90 %) were i...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-014-0340-4
更新日期：2015-01-01 00:00:00
abstract：:Lactobacillus bulgaricus 59 and L. casei RTS and their respective nitrosoguanidine-induced mutants were estimated for titratable and volatile acidities, production of biacetyl and acetoin and for proteolytic activity. Both the mutants showed a marked increase in proteolytic activity as compared to the parents. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02897216
更新日期：1980-01-01 00:00:00
abstract：:Vancomycin is often used in orthopedic surgery as a local prophylaxis of bacterial infection. The aim of this work was to compare the release of vancomycin and its biologically inactive crystalline degradation products (CDP-1) during in vitro experiments from different types of local antibiotic delivery systems (bone ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-019-00752-w
更新日期：2020-06-01 00:00:00
abstract：:The sorption ability of Candida utilis biomass for cadmium ions with accumulating competence of dried cells and cells in alginate was compared. After an optimization of process conditions (pH 5.5, biomass concentration 1 g/L and c0 50 mg/L), the cadmium sorption capacity of dried yeast biomass was perceptibly higher t...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931807
更新日期：2006-01-01 00:00:00
abstract：:Sixty four biopsy specimens of oral Candida leukoplakias were examined. Histological sections were stained with periodic acid-Schiff (PAS) reagent and by Grocott's silver method. C. albicans isolates were cultivated from all the patients but fungal hyphae were proved histologically only in 23 of them. PAS-positive and...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814437
更新日期：2001-01-01 00:00:00
abstract：:Genome projects produce a huge amount of sequence information. As a result, the focus of genomics research is turning toward deduction of functional information about newly discovered genes. Thus structural genomics paves the way for a new discipline called functional genomics by providing the information required for...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/BF02931546
更新日期：2004-01-01 00:00:00
abstract：:The prevalence of extended-spectrum beta-lactamases (ESBL) was determined among isolates of Escherichia coli (n = 63) isolated from hospitalized (43) and healthy (20) children. Ten isolates (21%) were ESBL-positive for two screening tests, the double disk-synergy test and the Oxoid Combination Disk method. One ESBL-po...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02930963
更新日期：2003-01-01 00:00:00
abstract：:Uropathogenic Escherichia coli (UPEC) isolates contain large genomic segments, termed pathogenicity islands (PAIs), that contribute to their virulence. A total of 150 UPEC and 50 commensal E. coli isolates from outpatients were investigated for antimicrobial susceptibility and the presence of eight PAI markers. One hu...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-015-0433-8
更新日期：2016-05-01 00:00:00
abstract：:Streptococcus dysgalactiae subsp. equisimilis (SDSE), belonging to the group C and G streptococci, are human pathogens reported to cause clinical manifestations similar to infections caused by Streptococcus pyogenes. To scrutinize the distribution of gene coding for S. pyogenes virulence factors in SDSE, 255 isolates ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-018-0595-2
更新日期：2018-09-01 00:00:00
abstract：:A small-colony forming variant of Escherichia coli with a mutation in the ncf gene was analysed. The alternation of the protein composition in the cytoplasmic membrane and the interaction with K and E group colicins indicated a membrane mutation. The effect of this mutation on some membrane-bound processes, the activi...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02885601
更新日期：1977-01-01 00:00:00
abstract：:A total of 20 putative strains of Erwinia amylovora originating from 11 samples of host plants with symptoms of fire blight were analyzed in detail using commercial polyclonal antibodies in immunochemical tests. Fourteen strains reacted negatively in all tests; 6 strains reacted positively with a polyclonal antibody f...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931569
更新日期：2005-01-01 00:00:00
abstract：:An extracellular acid phosphatase isolated from the culture of a wild strain Aspergillus niger, producing the dephosphorylating 3-phytase, was obtained in a homogeneous form by sequential application of ultrafiltration through PS 50 membrane, gel filtration on Sephadex G-100 and ion exchange chromatography on DEAE-Sep...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931618
更新日期：2006-01-01 00:00:00
abstract：:Changes in leukocyte counts and in the gut microflora of laboratory rats irradiated with single whole-body dose of gamma rays (5.0 Gy) were determined. The number of leukocytes was lower especially 1 and 2 weeks after irradiation. A significant decrease in lymphocytes was observed 1 week and in monocytes 1 and 2 weeks...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02818710
更新日期：2002-01-01 00:00:00
abstract：:Using an in vitro cytotoxicity assay (51Cr-release), it was shown that bilirubin exerts a cytotoxic effect on both adult and newborn human lymphocytes after a 1-d incubation of cells with bilirubin. The effect of bilirubin on human granulocytes was less pronounced; the association of the cytotoxic effect with a functi...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02923520
更新日期：1985-01-01 00:00:00
abstract：:Different mechanistic models for potassium ion uptake are analyzed by an equilibrium-thermodynamic formalism in terms of their comparative efficiency in setting chemical potential differences of the potassium ion of different magnitudes across the plasma membrane of lower eukaryotic cells. The possible adaptive advant...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/BF02925852
更新日期：1988-01-01 00:00:00
abstract：:The minimum inhibitory concentrations (MICs) of 24 antibiotics were determined for 45 Stenotrophomonas maltophilia strains by the microdilution method at 37 and 30 degrees C (after 24 h and 48 h of incubation). The isolates were obtained from mouth swabs and pus of 116 captive snakes whereas the identical strains (bas...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-010-0014-9
更新日期：2010-01-01 00:00:00
