Abstract:
:In the present study, three Arcanobacterium pluranimalium strains isolated from bovine milk samples of three cows of three farms (two cows with subclinical mastitis) could successfully be identified by phenotypical investigations, by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis and genotypically by sequencing the molecular targets 16S rDNA, 16S-23S rDNA intergenic spacer region (ISR), the β subunit of bacterial RNA polymerase encoding gene rpoB, the glyceraldehyde 3-phosphate dehydrogenase encoding gene gap, the elongation factor tu encoding gene tuf, and the pluranimaliumlysin encoding gene pla. The latter could also be identified by a loop-mediated isothermal amplification (LAMP) assay. The presented phenotypic and genotypic approaches might support the identification of A. pluranimalium in future and might help to understand the role this species plays in bovine mastitis.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Wickhorst JP,Hassan AA,Sammra O,Huber-Schlenstedt R,Lämmler C,Prenger-Berninghoff E,Timke M,Abdulmawjood Adoi
10.1007/s12223-016-0450-2subject
Has Abstractpub_date
2016-09-01 00:00:00pages
405-12issue
5eissn
0015-5632issn
1874-9356pii
10.1007/s12223-016-0450-2journal_volume
61pub_type
杂志文章abstract::Mineral nutrition of crop plants is one of the major challenges faced by modern agriculture, particularly in arid and semi-arid regions. In alkaline calcareous soils, the availability of phosphorus and zinc is critically less due to their fixation and precipitation as complexes. Farmers use fertilizers to fulfill crop...
journal_title:Folia microbiologica
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更新日期:2020-10-24 00:00:00
abstract::Using mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine, a number of homoserine auxotrophs have been isolated from a glutamate-producing Arthrobacter globiformis excreting L-lysine in good amounts. For further improvement, mutants resistant to the lysine analog S-(2-aminoethyl)-L-cysteine have been isolated from h...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02935823
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abstract::Pseudomonas aeruginosa is an opportunistic pathogen that causes serious infections, especially in patients with immunodeficiency. It exhibits multiple mechanisms of resistance, including efflux pumps, antibiotic modifying enzymes and limited membrane permeability. The primary reason for the development of novel therap...
journal_title:Folia microbiologica
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journal_title:Folia microbiologica
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更新日期:2020-08-01 00:00:00
abstract::Moderately halophilic bacteria utilizing phenol as the sole carbon source were isolated by selective enrichment from sea water. The isolate (Gram-negative motile rods) was identified as Deleya venusta. It grew well in the presence of up to 1600 mg/L of phenol and 8% NaCl under aerobic conditions. When the cells were t...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02815617
更新日期:2001-01-01 00:00:00
abstract::Investigation of the dependence of cell yield of Candida boidinii in a chemostat and specific growth rate in a turbidostat on temperature was carried out using methanol as substrate. Each of the curves obtained had only one maximum, the optimum temperature for yield (Ys) and for specific growth rate (micron) being som...
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doi:10.1007/BF02885600
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abstract::When investigating the effect of aeration on the utilization of a lipid substrate from the cultivation broth and production of salinomycin in Streptomyces albus it was demonstrated that a higher aeration results in a better utilization of soya oil and a higher production of the antibiotic. ...
journal_title:Folia microbiologica
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doi:10.1007/BF02879490
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abstract::Due to the nature of Vibrio vulnificus infections (i.e., gastroenteritis and septicemia), only very few studies of a biofilm-associated form in this pathogen's life cycle have been conducted. We proposed that biofilm production by clinical strains of V. vulnificus would be higher than by environmental strains. Biofilm...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-019-00761-9
更新日期:2020-06-01 00:00:00
abstract::It is proposed that the transcription of the c-2 repressor gene initiates at two promoters, pre and prm, similarly to the transcription of the c-I gene of lambda phage. In the P22 phage, these promoters are defined by mutations of c54 and K5. ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02876780
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abstract::Numerous antibodies with a known mechanism of action are utilized as possible means for studying morphogenesis and differentiation. Inhibitors of biosynthesis of nucleic acids and proteins, compounds intervening with the synthesis and/or function of cell walls and membranes or compounds influencing the energy metaboli...
journal_title:Folia microbiologica
pub_type: 杂志文章,评审
doi:10.1007/BF02897219
更新日期:1980-01-01 00:00:00
abstract::Structural and functional properties of prokaryotic DNA methyltransferases are summarized. The different aspects of the role of DNA methylation which influences DNA-protein interaction in restriction and modification of DNA and in mismatch repair, DNA replication and gene expression are discussed. ...
journal_title:Folia microbiologica
pub_type: 杂志文章,评审
doi:10.1007/BF02815658
更新日期:1992-01-01 00:00:00
abstract::As a part of a program to develop starter cultures aiding in the spoilage control and sanitation of African fermented foods, a cereal-based food ('ogi' and its solid form 'agidi' or 'eko') was prepared using a bacteriocin-producing Lactobacillus strain as the starter culture. The survival of an enterotoxigenic Escheri...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02898642
更新日期:1997-01-01 00:00:00
abstract::Rumen ciliate protozoa intensively engulf bacteria. However, their ability to utilize murein which is the main polysaccharide of bacterial cell wall has hardly been recognized. The present study concerns the ability of the rumen protozoa Diploplastron affine to digest and ferment murein. The ciliates were isolated fro...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-010-0047-0
更新日期:2010-07-01 00:00:00
abstract::Excessive use of antibiotics contributes to the selection of resistant bacteria and intestinal colonization with multiresistant pathogens poses a risk factor for subsequent infections. The present study assessed vancomycin-resistant enterococci (VRE) carriage rates in patients admitted to our tertiary care hospital. S...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-019-00751-x
更新日期:2020-06-01 00:00:00
abstract::The aim of our study was to find out the optimal conditions for short-term storage of cerebrospinal fluid (CSF) samples for direct diagnosis of Lyme disease. A mixture of Borrelia-negative CSFs spiked with a defined amount of cultured Borrelia garinii was used. Borrelia stability was investigated over 7 days at four d...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-015-0408-9
更新日期:2016-01-01 00:00:00
abstract::A collection of 34 lactococcal strains were characterized using the polymerase chain reaction (PCR) for the acmA gene, and for the 16S rDNA gene, and DNA fingerprinting methods for randomly amplified polymorphic DNA (RAPD) and repetitive extragenic palindrome-PCR (rep-PCR). PCR experiments corroborated the genotypic i...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931464
更新日期:2005-01-01 00:00:00
abstract::The present paper reports a modified method for isolation of lysostaphin--a bacteriolytic agent with specific affinity for staphylococcal cell wall. The proposed purification scheme includes three steps. The first procedure is ultrafiltration through a membrane filter giving a yield of 75.6%. The result of ultrafiltra...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02814386
更新日期:1993-01-01 00:00:00
abstract::This study presents the effects of the probiotic and enterocin M-producing strain Enterococcus faecium AL41 on microbiota, phagocytic activity (PA), oxidative stress, performance and biochemical parameters when applied individually or in combination with Eleutherococcus senticosus in rabbits. The novelty of the study ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-015-0423-x
更新日期:2016-03-01 00:00:00
abstract::The color of light (white, red, blue, and green) had a significant effect on the growth and reproductive processes (both in the nucleocytoplasmic and chloroplast compartment of the cells) in synchronous cultures of Scenedesmus obliquus. This effect decreased in the order red > white > blue > green. In the same order, ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931828
更新日期:2006-01-01 00:00:00
abstract::An experimental model in white mice, infected with a mildly virulent strain of Klebsiella pneumoniae, was elaborated for studies on local immunity of the respiratory tract. Instillation of klebsiella into the supralaryngeal space of anaesthetised animals proved to be more suitable than the commonly used method of intr...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02879494
更新日期:1983-01-01 00:00:00
abstract::A gram-negative bacterium GXGL-4A was originally isolated from maize roots. It displayed nitrogen-fixing (NF) ability under nitrogen-free culture condition, and had a significant promotion effect on cucumber growth in the pot inoculation test. The preliminary physiological and biochemical traits of GXGL-4A were charac...
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doi:10.1007/s12223-018-0608-1
更新日期:2018-11-01 00:00:00
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journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-014-0340-4
更新日期:2015-01-01 00:00:00
abstract::One hundred clinical isolates of Pseudomonas aeruginosa were checked for their sensitivity towards silver nitrate. Majority of the isolates were resistant at 20 mg/L and the resistance decreased with increasing concentration of silver nitrate, only 5% of the organisms showed resistance above 70 mg/L. These silver-resi...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02820752
更新日期:1989-01-01 00:00:00
abstract::A quarantine organism, "Candidatus Phytoplasma mali," is the causal agent of apple proliferation, one of the most important apple diseases in Europe. The genetic diversity of this pathogen in Central and Southern Europe has already been reported; however, almost no data exists from Eastern Europe. In this study, "Ca. ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-018-0660-x
更新日期:2019-05-01 00:00:00
abstract::Propionate at concentrations up to 0.05% decreased at 25 degrees C the growth and sporulation of Penicillium aurantiogriseum. The standard size of conidiogenous structures (metulae, phialids) and conidia was diminished. The effect was more pronounced at a higher temperature (30 degrees C). Inhibition of ochratoxin pro...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02814202
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abstract::Mathematical expressions were derived for the dilution rate and concentration of algae determining the maximum biomass production in a semicontinuous algal culture. ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02926655
更新日期:1979-01-01 00:00:00
abstract::The properties of the 37400 oncogenic strain of Agrobacterium tumefaciens are described. This strain was derived from the VI lysogenic strain originally isolated by Hamilton from a Zinnia elegans tumour. Strain 37400 has a number of properties which render it suitable for quantitative and genetic studies. It is cured ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02876963
更新日期:1976-01-01 00:00:00
abstract::Cryptococcus neoformans is encapsulated yeast that causes cryptococcosis. The cryptococcal meningitis may cause neuropsychiatric symptoms. Here, we evaluated the in vitro activity of amphotericin B (AMB), chlorpromazine (CLOR), and sertraline (SERT) alone or in combination against clinical isolates of C. neoformans co...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-016-0449-8
更新日期:2016-09-01 00:00:00
abstract::Poly-gamma-L-glutamic acid was synthesized in optimized fermentor cultivation of Bacillus licheniformis. The polypeptide obtained was esterified using benzyl- and butylbromides. Benzyl esters with 30, 60 and 100% degree of esterification were prepared. Solubility of the products in dimethyl sulfoxide, chloroform, meth...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02818530
更新日期:2001-01-01 00:00:00
abstract::In the present study, 193 Aspergillus strains were isolated from a total of 100 soil samples of pistachio orchards, which all of them were identified as Aspergillus flavus as the most abundant species of Aspergillus section Flavi existing in the environment. Approximately 59%, 81%, and 61% of the isolates were capable...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-011-0090-5
更新日期:2012-01-01 00:00:00