Phenotypic and genotypic approach to characterize Arcanobacterium pluranimalium isolated from bovine milk samples.

Abstract:

:In the present study, three Arcanobacterium pluranimalium strains isolated from bovine milk samples of three cows of three farms (two cows with subclinical mastitis) could successfully be identified by phenotypical investigations, by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis and genotypically by sequencing the molecular targets 16S rDNA, 16S-23S rDNA intergenic spacer region (ISR), the β subunit of bacterial RNA polymerase encoding gene rpoB, the glyceraldehyde 3-phosphate dehydrogenase encoding gene gap, the elongation factor tu encoding gene tuf, and the pluranimaliumlysin encoding gene pla. The latter could also be identified by a loop-mediated isothermal amplification (LAMP) assay. The presented phenotypic and genotypic approaches might support the identification of A. pluranimalium in future and might help to understand the role this species plays in bovine mastitis.

journal_title

Folia microbiologica

authors

Wickhorst JP,Hassan AA,Sammra O,Huber-Schlenstedt R,Lämmler C,Prenger-Berninghoff E,Timke M,Abdulmawjood A

doi

10.1007/s12223-016-0450-2

subject

Has Abstract

pub_date

2016-09-01 00:00:00

pages

405-12

issue

5

eissn

0015-5632

issn

1874-9356

pii

10.1007/s12223-016-0450-2

journal_volume

61

pub_type

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