Properties of the cured oncogenic strain 37400 of Agrobacterium tumefaciens.

Abstract:

:The properties of the 37400 oncogenic strain of Agrobacterium tumefaciens are described. This strain was derived from the VI lysogenic strain originally isolated by Hamilton from a Zinnia elegans tumour. Strain 37400 has a number of properties which render it suitable for quantitative and genetic studies. It is cured of prophages and can serve as a universal sensitive indicator for a number of phages isolated from various lysogenic strains of Agrobacterium tumefaciens. Its good growth properties in synthetic media and at elevated temperatues enable the isolation of auxotrophic mutants and temperature sensitive phage mutants. Preliminary experiments show that strain 37400 will serve as suitable starting material for conjugation experiments under defined conditions.

journal_title

Folia microbiologica

authors

Bezdĕk M,Reich J,Tkadlecek L

doi

10.1007/BF02876963

subject

Has Abstract

pub_date

1976-01-01 00:00:00

pages

371-7

issue

5

eissn

0015-5632

issn

1874-9356

journal_volume

21

pub_type

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