Abstract：
:Streptococcus dysgalactiae subsp. equisimilis (SDSE), belonging to the group C and G streptococci, are human pathogens reported to cause clinical manifestations similar to infections caused by Streptococcus pyogenes. To scrutinize the distribution of gene coding for S. pyogenes virulence factors in SDSE, 255 isolates were collected from humans infected with SDSE in Vellore, a region in southern India, with high incidence of SDSE infections. Initial evaluation indicated SDSE isolates comprising of 82.35% group G and 17.64% group C. A multiplex PCR system was used to detect 21 gene encoding virulence-associated factors of S. pyogenes, like superantigens, DNases, proteinases, and other immune modulatory toxins. As validated by DNA sequencing of the PCR products, sequences homologous to speC, speG, speH, speI, speL, ssa and smeZ of the family of superantigen coding genes and for DNases like sdaD and sdc were detected in the SDSE collection. Furthermore, there was high abundance (48.12% in group G and 86.6% in group C SDSE) of scpA, the gene coding for C5a peptidase in these isolates. Higher abundance of S. pyogenes virulence factor genes was observed in SDSE of Lancefield group C as compared to group G, even though the incidence rates in former were lower. This study not only substantiates detection of S. pyogenes virulence factor genes in whole genome sequenced SDSE but also makes significant contribution towards the understanding of SDSE and its increasing virulence potential.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Babbar A,Itzek A,Pieper DH,Nitsche-Schmitz DPdoi
10.1007/s12223-018-0595-2subject
Has Abstractpub_date
2018-09-01 00:00:00pages
581-586issue
5eissn
0015-5632issn
1874-9356pii
10.1007/s12223-018-0595-2journal_volume
63pub_type
杂志文章abstract：:Inactivation of seven strains derived from Escherichia coli B differing in their capacity to repair damage to their DNA (exc, pol, rec) after irradiation with far (254 nm) and middle and near (300 to 380 and 320--400 nm) UV light was investigated. The same bacterial strains were also used as hosts for the UV-irradiate...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02876690
更新日期：1980-01-01 00:00:00
abstract：:3-(1,1-Dioxadibenzothien-4-oyl)acrylic acid (1) was condensed with compounds containing active methylene groups under Michael reaction conditions to furnish the Michael adducts (lactones 2a-c, lactams 3a-c, ketones 4a,b). The behavior of these adducts toward the action of hydrazine hydrate were investigated. The compo...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02817670
更新日期：2002-01-01 00:00:00
abstract：:The uvrE (E. coli KS 114) cells carry a mutation in the gene that codes for helicase II. This is the protein responsible for replicative unwinding of double-helical DNA. The repair mode of such cells may be altered as compared with the wild type. The survival of uvrE cells during postirradiation incubation under inhib...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02922491
更新日期：1985-01-01 00:00:00
abstract：:The screening of 17 SAg genes of S. aureus isolated from the sputum of cystic fibrosis (CF) patients revealed that among 47 genetically different strains, 39 (83 %) carried SAg genes. Superantigens forming enterotoxin gene cluster were detected in 20 strains. The 2nd most common superantigen type was selk detected in ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931628
更新日期：2006-01-01 00:00:00
abstract：:The geographical distribution of Bacillus anthracis strains and isolates bearing some of the same genetic markers as the Amerithrax Ames isolate was examined and evaluated. At least one mechanism for the horizontal movement of genetic markers was shown amongst isolates and closely related species and the effect of suc...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-008-0074-2
更新日期：2008-01-01 00:00:00
abstract：:Mitochondria isolated from Neurospora crassa were purified by centrifugation in a Percoll density gradient. Enzyme activities and cytochrome differential spectra revealed a high purity of the mitochondria. As compared with a crude mitochondrial fraction the purified mitochondria exhibited a high respiratory activity a...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02879491
更新日期：1983-01-01 00:00:00
abstract：:Propionate at concentrations up to 0.05% decreased at 25 degrees C the growth and sporulation of Penicillium aurantiogriseum. The standard size of conidiogenous structures (metulae, phialids) and conidia was diminished. The effect was more pronounced at a higher temperature (30 degrees C). Inhibition of ochratoxin pro...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814202
更新日期：1995-01-01 00:00:00
abstract：:Three types of heterogeneous preparations and four types of preparations of polysaccharide nature were obtained in studies aimed at the isolation of active compounds from Aspergillus flavus conidia bearing their biological stimulatory activity. Extraction with trichloroacetic acid at 0 degrees C yielded a preparation ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02927425
更新日期：1981-01-01 00:00:00
abstract：:Activity of purified alanylaminopeptidase of Pseudomonas sp. measured in the presence of the alanine derivative of 2-naphthoic acid (NA-Ala) is inhibited by 1,10-phenanthroline, EDTA, bestatin and amastatin; this finding supports the conclusion that this enzyme is a metallo-aminopeptidase. A decrease of its activity i...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02817643
更新日期：2002-01-01 00:00:00
abstract：:There is a growing interest in the use of bioinoculants to assist mineral fertilizers in improving crop production and yield. Azotobacter and Pseudomonas are two agriculturally relevant strains of bacteria which have been established as efficient bioinoculants. An experiment involving addition of graded concentrations...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-017-0495-x
更新日期：2017-05-01 00:00:00
abstract：:An extracellular acid phosphatase isolated from the culture of a wild strain Aspergillus niger, producing the dephosphorylating 3-phytase, was obtained in a homogeneous form by sequential application of ultrafiltration through PS 50 membrane, gel filtration on Sephadex G-100 and ion exchange chromatography on DEAE-Sep...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931618
更新日期：2006-01-01 00:00:00
abstract：:Microorganisms that cause chronic infections exist predominantly as surface-attached stable communities known as biofilms. Microbial cells in biofilms are highly resistant to conventional antibiotics and other forms of antimicrobial treatment; therefore, modern medicine tries to develop new drugs that exhibit anti-bio...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-018-0633-0
更新日期：2019-01-01 00:00:00
abstract：:One hundred clinical isolates of Pseudomonas aeruginosa were checked for their sensitivity towards silver nitrate. Majority of the isolates were resistant at 20 mg/L and the resistance decreased with increasing concentration of silver nitrate, only 5% of the organisms showed resistance above 70 mg/L. These silver-resi...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02820752
更新日期：1989-01-01 00:00:00
abstract：:Five arsenic-resistant bacterial strains (designated MP1400, MP1400a, MP1400d, APSLA3, and BPSLA3) were isolated from soils collected at the Alps region (Italy), which showed no contamination by arsenic. Phylogenetic analysis of the 16S rRNA gene sequences assigned them to the genera Pseudomonas and Bacillus. Bacillus...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-011-0010-8
更新日期：2011-01-01 00:00:00
abstract：:The temperature dependence of fluorescence anisotropy, lifetime and differential tangent of 1,6-diphenyl-1,3,5-hexatriene (DPH) and its polar trimethylammonium derivative (TMA-DPH) were investigated in cytoplasmic membranes of Bacillus subtilis. The fluorescence parameters were compared in the two types of membranes p...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02821406
更新日期：1990-01-01 00:00:00
abstract：:The vacA and cagA genotypes of Helicobacter pylori exhibited distinct geographic distribution and correlation with severity of disease. In the above genotypes (obtained from 150 H. pylori-positive patients--139 with gastritis, 10 with ulcer and 1 patient with gastric cancer) combinations vacA s1/m1 and s2/m2 were dete...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02932113
更新日期：2007-01-01 00:00:00
abstract：:During cultivation in a minimal medium with glucose Alternaria tenuissima and Aspergillus vesicolor produce constitutively alpha- and beta-glucanases. Fractions of beta-1,3-glucanases exhibiting affinity for laminarin were separated by means of gel filtration chromatography. Two neutral beta-1,3-glucanases with affini...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02876394
更新日期：1980-01-01 00:00:00
abstract：:Five trisubstituted quinazolones and eight trisubstituted quinazoline-4-thiones have been tested for antibacterial effects by a microdilution method. Four derivatives exerted a significant effect on E. coli, P. aeruginosa, S. aureus and B. subtilis (IC50 < 100 mg/L). In the bacterium P. aeruginosa six quinazolines sho...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02817411
更新日期：2000-01-01 00:00:00
abstract：:Secreted aspartyl proteinase (Sap) distribution among different C. albicans isolates was determined using SAP-specific primers in polymerase chain reaction (PCR) assay. SAP1, SAP2, and SAP3 were detected in 13 of 40 (32.5%), SAP4 in 38/40 (95%), SAP5 were detected in 30/40 (75%), SAP6 in 23/40 (57.5%) of C. albicans s...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931422
更新日期：2005-01-01 00:00:00
abstract：:Inducible pigmentation changes were observed in pigmented strains of Brevibacterium sp. M27 and B. flavum treated with N-methyl-N'-nitro-N-nitrosoguanidine. The highest frequency of induction was reached already at a survival of 30-40% with the maximal yield of 6-10%. As compared with the initial yellow colour, three ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02928007
更新日期：1988-01-01 00:00:00
abstract：:Effect of oxygen and metabolic substrates (glucose, ethanol) on the catalase activity of anaerobically grown Schizosaccharomyces pombe cells was assessed and compared with that of Saccharomyces cerevisiae in order to determine the catalase activity regulation in S. pombe. In contrast to S. cerevisiae, the total catala...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02818575
更新日期：1998-01-01 00:00:00
abstract：:Extrusion of metabolites (glycerol, lactic, malic, and succinic acid) during the medium acidification caused by resting baker's yeast supplied with 200 mM glucose was studied under aerobic and anaerobic conditions and in the absence and presence of 14 mM KCl. The maximum levels of glycerol and of the sum of acids (abo...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02876611
更新日期：1980-01-01 00:00:00
abstract：:A new strain of Bacillus sp. was isolated from a hot water spring in India. This strain generated a high activity of extracellular beta-galactosidase at 37 degrees C in shake flasks. The beta-galactosidase activity was found to increase continuously but the production rate was slower than with some other organisms rep...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02903706
更新日期：1999-01-01 00:00:00
abstract：:The microflora of the chernozem soil mineralized 62% of the lactose retained on a column consisting of three 10-g layers of the soil at a daily flow of 48 mg of the sugar. Only 45% of the sugar was mineralized when the daily flow was 136 mg. The highest value of the beta-galactosidase activity in the system of heteroc...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02927259
更新日期：1981-01-01 00:00:00
abstract：:The root nodules of Phaseolus mungo (a herbaceous leguminous pulse) contained a high amount of 3-indolylacetic acid (IAA). A tryptophan pool present in the nodule might play the role of precursor for IAA production. From the root nodule a Rhizobium sp. was isolated. The symbiont produced a large amount of IAA (142 mic...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-008-0054-6
更新日期：2008-01-01 00:00:00
abstract：:Pullulanase of Klebsiella oxytoca is one of a wide variety of extracellular proteins that are secreted by Gram-negative bacteria by the complex main terminal branch (MTB) of the general secretory pathway. The roles of some of the 14 components of the MTB are now becoming clear. In this review it is proposed that most ...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/BF02818976
更新日期：1997-01-01 00:00:00
abstract：:Biodegradation of two amphiphilic "soft" antimicrobially active derivatives of lauric (dodecanoic) acid, a quaternary ammonium salt and an amine oxide bearing an amide or ester group, was followed using microorganisms from activated sludge. Primary biodegradation was determined by ion-selective electrodes, total biode...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814548
更新日期：1993-01-01 00:00:00
abstract：:Enterococcus faecalis is a ubiquitous bacterium of the gut that is observed in persistent periradicular infections. Its pathogenicity is associated with biofilm formation and the ability to survive under nutrient-poor (starvation) conditions. However, characteristics of chemical composition of biofilm cells developed ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-014-0319-1
更新日期：2014-09-01 00:00:00
abstract：:The chloramphenicol resistance gene from pSa was introduced into T-DNA of pTi T37 of Agrobacterium tumefaciens by cointegration with intermediary plasmid based on pBR322. The resulting intermediary vector was mobilized to A. tumefaciens T37 by conjugative plasmid pRK2. The RK2 plasmid also forms contegrates with pTi d...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02926824
更新日期：1986-01-01 00:00:00
abstract：:Nalidixic acid was used for describing more accurately the terminal replication region of the Mycobacterium phlei chromosome. Cell division in synchronized cultures was not sensitive to this acid any more between 185-190 min, i.e. about 10 min after replication of the ser gene, the last of 24 genes of the replication ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02927251
更新日期：1981-01-01 00:00:00
