Abstract：
:A new strain of Bacillus sp. was isolated from a hot water spring in India. This strain generated a high activity of extracellular beta-galactosidase at 37 degrees C in shake flasks. The beta-galactosidase activity was found to increase continuously but the production rate was slower than with some other organisms reported in the literature. There were noteworthy differences in the time-domain profiles of bacterial concentration and beta-galactosidase activity when the starting concentration of substrate (glucose) was tripled from 10 g/L. These differences may be explained in terms of the relative rates of enzyme synthesis and its diffusion across the cell wall. The enzyme produced by this organism is more stable than other beta-galactosidases; its half-life is 408 h at 50 degrees C and 94 h at 55 degrees C, while the reported enzymes showed perceptible loss of activity within 2 h.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Sani RK,Chakraborti S,Sobti RC,Patnaik PR,Banerjee UCdoi
10.1007/BF02903706keywords:
subject
Has Abstractpub_date
1999-01-01 00:00:00pages
367-71issue
4eissn
0015-5632issn
1874-9356journal_volume
44pub_type
杂志文章abstract：:Rumen ciliate protozoa intensively engulf bacteria. However, their ability to utilize murein which is the main polysaccharide of bacterial cell wall has hardly been recognized. The present study concerns the ability of the rumen protozoa Diploplastron affine to digest and ferment murein. The ciliates were isolated fro...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-010-0047-0
更新日期：2010-07-01 00:00:00
abstract：:There is a growing interest in the use of bioinoculants to assist mineral fertilizers in improving crop production and yield. Azotobacter and Pseudomonas are two agriculturally relevant strains of bacteria which have been established as efficient bioinoculants. An experiment involving addition of graded concentrations...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-017-0495-x
更新日期：2017-05-01 00:00:00
abstract：:Glycerol-based medium (BM) with and without the addition of 1 g/L ascorbic acid (Asc) and/or 100 mg/L (+/-)-catechin (Cat) was tested for the storage of three commercial wine yeasts at -20 degrees C. The medium supplemented with Asc was also used to store 706 strains to verify the maintenance of the liquid state. A de...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-009-0036-3
更新日期：2009-01-01 00:00:00
abstract：:The root nodules of Phaseolus mungo (a herbaceous leguminous pulse) contained a high amount of 3-indolylacetic acid (IAA). A tryptophan pool present in the nodule might play the role of precursor for IAA production. From the root nodule a Rhizobium sp. was isolated. The symbiont produced a large amount of IAA (142 mic...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-008-0054-6
更新日期：2008-01-01 00:00:00
abstract：:Three methods were used for the isolation of fungi in the present investigation: the dilution-plate method, hair-baiting technique with horse hairs and the "exposed plate" method. Sabouraud's glucose agar at 28 degrees C was also used as isolation medium. 102 species and 2 species varieties belonging to 36 genera were...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02821282
更新日期：1990-01-01 00:00:00
abstract：:The biosynthetic gene cluster of porothramycin, a sequence-selective DNA alkylating compound, was identified in the genome of producing strain Streptomyces albus subsp. albus (ATCC 39897) and sequentially characterized. A 39.7 kb long DNA region contains 27 putative genes, 18 of them revealing high similarity with hom...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-014-0339-x
更新日期：2014-11-01 00:00:00
abstract：:Among 18 tested strains of Claviceps spp., 7 produced significant amounts of exocellular polysaccharide (EPS). The maximum production of EPS was found in fermentation broth of Claviceps viridis. The kinetics of growth, substrate consumption, and EPS production in the batch, aerobic, submerged culture of this fungus we...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02993470
更新日期：2003-01-01 00:00:00
abstract：:This work was aimed to develop a multiplex PCR assay to detect infectious agents such as Clavibacter michiganensis subsp. michiganensis, Fusarium sp, Leveillula taurica, and begomoviruses in tomato (Solanum lycopersicum) plants. Specific primer sets of each pathogen were designed based on intergenic ribosomal RNA sequ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-012-0206-6
更新日期：2013-07-01 00:00:00
abstract：:A new metabolite was isolated from the culture of Penicillium rugulosum and its structure was determined from physico-chemical data. Accompanying metabolites skyrin and rugulosin were characterized by UV, IR, CD, mass and NMR spectra. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02876598
更新日期：1978-01-01 00:00:00
abstract：:Many serious diseases caused by Staphylococcus aureus appear to be associated with biofilms. Therefore, we investigated the biofilm-forming ability of the methicillin-resistant S. aureus (MRSA) isolates collected from hospitalized patients. As many as 96 % strains had the ability to form biofilm in vitro. The majority...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-012-0175-9
更新日期：2013-01-01 00:00:00
abstract：:Cultures of 33 basidiomycete strains out of 35 tested were viable with unchanged characteristics after four years of maintenance on perlite in cryovials. These cultures can be a good substitute for agar cultures in long-term maintenance of fungi. For comparison, the storage under oil was evaluated but it turned out to...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-008-0084-0
更新日期：2008-01-01 00:00:00
abstract：:The efficacy of the agents was evaluated on the basis of minimum inhibitory concentration (MIC) and time-kill curve analysis in Pseudomonas aeruginosa, Staphylococcus aureus, Acinetobacter baumannii, Mycobacterium smegmatis, and Citrobacter braakii; MIC was found to be 0.5, 4.0, 0.015625, 0.0078125, and 0.0625 mg/L in...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-009-0054-1
更新日期：2009-09-01 00:00:00
abstract：:beta-Glucosidase from the fungus Thermoascus aurantiacus grown on semi-solid fermentation medium (using ground corncob as substrate) was partially purified in 5 steps--ultrafiltration, ethanol precipitation, gel filtration and 2 anion exchange chromatography runs, and characterized. After the first anion exchange chro...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02818672
更新日期：2002-01-01 00:00:00
abstract：:Sodium fluoride showed pH-dependent physiological responses in the two test microalgae Anabaena khannae and Chlorococcum humicola. A. khannae showed severe membrane damage with fluoride at low pH with leakage of pigments and electrolytes. Annihilation of photosynthesis along with inhibition in 14C uptake was observed ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931045
更新日期：2004-01-01 00:00:00
abstract：:A comparative examination of reduced methyl [MV·](+) and benzyl [BV·](+) viologens (as artificial electron donors for quantitative estimation of the respiratory periplasmic (Nap) and membrane-embedded (Nar) nitrate reductases) using a newly constructed nap mutant strain of Paracocccus denitrificans was done. The activ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-011-0004-6
更新日期：2011-01-01 00:00:00
abstract：:Changes in leukocyte counts and in the gut microflora of laboratory rats irradiated with single whole-body dose of gamma rays (5.0 Gy) were determined. The number of leukocytes was lower especially 1 and 2 weeks after irradiation. A significant decrease in lymphocytes was observed 1 week and in monocytes 1 and 2 weeks...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02818710
更新日期：2002-01-01 00:00:00
abstract：:The synergistic anticandidal activity of three diketopiperazines [cyclo-(L-Pro-L-Leu) (1), cyclo-(D-Pro-L-Leu) (2), and cyclo-(D-Pro-L-Tyr) (3)] purified from a Bacillus sp. N strain associated with entomopathogenic nematode Rhabditis (Oscheius) in combination with amphotericin B and clotrimazole was investigated usin...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-013-0234-x
更新日期：2013-11-01 00:00:00
abstract：:Using fluorescein-labelled antibodies against gamma, mu and alpha chains, Ig-containing cells in palatine tonsils were studied in 120 patients. The aim of this study was to determine the most frequently repeated typical findings as regards the numbers and localisation of these cells in tonsils and to confront the data...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02927432
更新日期：1981-01-01 00:00:00
abstract：:A hexokinase preparation was obtained from a Saccharomyces cerevisiae mutant strain deficient in glucosephosphate isomerase (GPI) and mannosephosphate isomerase (MPI) by precipitation with ammonium sulfate. The supernatant fraction corresponding to 40-60 % saturation showed the lowest content in GPI and MPI activity. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02927364
更新日期：1981-01-01 00:00:00
abstract：:Over the years, quinones or its derivatives have been extensively studied due to their broad therapeutic spectrum. However, due to the significant structural differences between the individual naturally occurring quinones, investigation of the precise mechanism of their action is essential. In this context, we have an...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-019-00722-2
更新日期：2020-02-01 00:00:00
abstract：:Submerged cultures of the low-production strain of Tolypocladium inflatum DSM 63544 formed a mixture of cyclosporins (CS) consisting of CS-A, CS-B, "CS-3" and "CS-4". Glucose, sucrose and maltose were highly favored for biomass production but provided a different physiological state necessary for CS biosynthesis. Not ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02884035
更新日期：1991-01-01 00:00:00
abstract：:Physiological and biochemical properties were tested in 45 isolates of Aspergillus egyptiacus (16 isolates), Emericella nidulans (16) and Aspergillus versicolor (13). The three fungal species exhibited common and similar features. The big similarity between A. egyptiacus and E. nidulans was greater than between A. egy...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814449
更新日期：1994-01-01 00:00:00
abstract：:Extrusion of metabolites (glycerol, lactic, malic, and succinic acid) during the medium acidification caused by resting baker's yeast supplied with 200 mM glucose was studied under aerobic and anaerobic conditions and in the absence and presence of 14 mM KCl. The maximum levels of glycerol and of the sum of acids (abo...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02876611
更新日期：1980-01-01 00:00:00
abstract：:The majority of antibiotics and substances with diverse biological activity used in medicine are produced by actinomycetes, nonfilamentous bacteria and fungi. Other microorganisms, such as myxobacteria, pseudomonads, nocardias, basidiomycetes, marine microorganisms, enterobacteria, halobacteria, hyperthermophiles etc....
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/BF02993460
更新日期：2003-01-01 00:00:00
abstract：:The vacA and cagA genotypes of Helicobacter pylori exhibited distinct geographic distribution and correlation with severity of disease. In the above genotypes (obtained from 150 H. pylori-positive patients--139 with gastritis, 10 with ulcer and 1 patient with gastric cancer) combinations vacA s1/m1 and s2/m2 were dete...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02932113
更新日期：2007-01-01 00:00:00
abstract：:Fibrobacter succinogenes is a major cellulolytic species in the rumen. On the basis of molecular data, this species can be classified into four phylogenetic groups. Recently gathered ecological and physiological data have revealed the importance of this species, particularly phylogenetic group 1, in rumen fiber digest...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/s12223-008-0024-z
更新日期：2008-01-01 00:00:00
abstract：:Effect of oxygen and metabolic substrates (glucose, ethanol) on the catalase activity of anaerobically grown Schizosaccharomyces pombe cells was assessed and compared with that of Saccharomyces cerevisiae in order to determine the catalase activity regulation in S. pombe. In contrast to S. cerevisiae, the total catala...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02818575
更新日期：1998-01-01 00:00:00
abstract：:A proteus strain inhibited mycelial growth of Fusarium oxysporum in vitro. Seed bacterization showed significant plant growth promotion and Fusarium-wilt suppression activity of Phaseolus mungo in a gnotobiotic system. The culture filtrate of this strain exhibited three prominent bands in UV-VIS spectra between 300 an...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02818723
更新日期：2000-01-01 00:00:00
abstract：:The plasmid R1drd-19 Km- and its derivative pON5300 were split by the PstI restriction enzyme; they differed in a 2.4 Mg/mol fragment which was present only in the pON5300. Both plasmids were present in 1-2 copies per chromosomal equivalent in E. coli JC5455 cells. The pON5300 is stable in its original mutagenized hos...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02879554
更新日期：1983-01-01 00:00:00
abstract：:Biodegradation of two amphiphilic "soft" antimicrobially active derivatives of lauric (dodecanoic) acid, a quaternary ammonium salt and an amine oxide bearing an amide or ester group, was followed using microorganisms from activated sludge. Primary biodegradation was determined by ion-selective electrodes, total biode...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814548
更新日期：1993-01-01 00:00:00
