Abstract：
:The synergistic anticandidal activity of three diketopiperazines [cyclo-(L-Pro-L-Leu) (1), cyclo-(D-Pro-L-Leu) (2), and cyclo-(D-Pro-L-Tyr) (3)] purified from a Bacillus sp. N strain associated with entomopathogenic nematode Rhabditis (Oscheius) in combination with amphotericin B and clotrimazole was investigated using the macrodilution method. The minimum inhibitory concentration and minimum fungicidal concentration of the diketopiperazines was compared with that of the standard antibiotics. The synergistic anticandidal activities of diketopiperazines with amphotericin B or clotrimazole were assessed using the checkerboard and time-kill methods. The results of the present study showed that the combined effects of diketopiperazines with amphotericin B or clotrimazole predominantly recorded synergistic (<0.5). Time-kill study showed that the growth of the Candida was completely attenuated after 12-24 h of treatment with 50:50 ratios of diketopiperazines and antibiotics. These results suggest that diketopiperazines combined with antibiotics may be microbiologically beneficial and not antagonistic. These findings have potential implications in delaying the development of resistance as the anticandidal effect is achieved with lower concentrations of both drugs (diketopiperazines and antibiotics). The cytotoxicity of diketopiperazines was also tested against two normal human cell lines (L231 lung epithelial and FS normal fibroblast) and no cytotoxicity was recorded for diketopiperazines up to 200 μg/mL. The in vitro synergistic activity of diketopiperazines with antibiotics against Candida albicans is reported here for the first time.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Kumar SN,Nambisan B,Mohandas C,Sundaresan Adoi
10.1007/s12223-013-0234-xsubject
Has Abstractpub_date
2013-11-01 00:00:00pages
475-82issue
6eissn
0015-5632issn
1874-9356journal_volume
58pub_type
杂志文章abstract：:Hydroxy, n-saturated, branched, dioic, and unsaturated fatty acids in six freshwater wild cyanobacteria (Chroococcus minutus, Lyngbya ceylanica, Merismopedia glauca, Nodularia sphaerocarpa, Nostoc linckia, and Synechococcus aeruginosus) collected from different lakes and springs of Israel have been identified by gas c...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931279
更新日期：2003-01-01 00:00:00
abstract：:Composition of sterol fractions of nystatin-resistant Candida maltosa strains was determined. Using UV-spectrometry, TLC and GLC-MS it was demonstrated that resistance to nystatin is connected with the composition alterations of yeast cell sterols. Block of different stages of ergosterol biosynthesis was revealed in s...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814494
更新日期：1991-01-01 00:00:00
abstract：:As phospholipases of mycoplasma species may play a role in the pathogenesis of respiratory tract and urogenital tract diseases Mycoplasma mycoides and Acholeplasma laidlawii were examined as to the production of phospholipase A2 (PLA) and attempts were made to purify and characterize it. Both species produced PLA. The...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814471
更新日期：1989-01-01 00:00:00
abstract：:Numerous antibodies with a known mechanism of action are utilized as possible means for studying morphogenesis and differentiation. Inhibitors of biosynthesis of nucleic acids and proteins, compounds intervening with the synthesis and/or function of cell walls and membranes or compounds influencing the energy metaboli...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/BF02897219
更新日期：1980-01-01 00:00:00
abstract：:Verotoxin-producing Escherichia coli (VTEC) are nowadays among the most important emerging group of food-borne pathogens (VTEC strains cause gastroenteritis that can be complicated by the hemorrhagic colitis or hemolytic uremic syndrome, HUS). Escherichia coli 026 producing verotoxin 2 was isolated and its identity co...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931454
更新日期：2005-01-01 00:00:00
abstract：:Lactobacillus pobuzihii is a novel species which has been previously found in pobuzihi (fermented cummingcordia), a traditional fermented food in Taiwan. However, the lactic acid bacteria (LAB) microflora in pobuzihi has not been studied in detail. In this study, LAB from pobuzihi were isolated, identified, and charac...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-012-0188-4
更新日期：2013-03-01 00:00:00
abstract：:Physiological and biochemical properties were tested in 45 isolates of Aspergillus egyptiacus (16 isolates), Emericella nidulans (16) and Aspergillus versicolor (13). The three fungal species exhibited common and similar features. The big similarity between A. egyptiacus and E. nidulans was greater than between A. egy...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814449
更新日期：1994-01-01 00:00:00
abstract：:Common variable immunodeficiency (CVID) comprises a heterogeneous group of patients with as yet undefined genetic defects. Patients with CVID have in common a decrease in the levels of one or more serum immunoglobulin isotypes and a severe defect in the production of specific antibodies. Typically, the patients suffer...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/BF02814744
更新日期：1995-01-01 00:00:00
abstract：:Ultrastructural changes induced by Rickettsia slovaca standard type (ST) and wild type (WT) were examined during their life cycle in L929 and Vero cells. R. slovaca invaded the cytoplasm of the host cell by phagocytosis on the 1st d p.i. Rickettsiae adhering to the cytoplasmic membrane were engulfed by cellular extens...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-009-0019-4
更新日期：2009-01-01 00:00:00
abstract：:For the first time, it was reported in France a cluster of autochthonous severe community-acquired (CA) infections due to the USA300 methicillin-resistant Staphylococcus aureus (MRSA) clone. The three cases belonged to the same family without any identified clue of abroad importation pathway. The domestic spread of US...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-014-0322-6
更新日期：2014-11-01 00:00:00
abstract：:Mutants of Streptomyces coeruleorubidus, blocked in the biosynthesis of anthracycline antibiotics of the daunomycine complex, were isolated from the production strains after treatment with UV light, gamma-radiation, nitrous acid, and after natural selection; according to their different biosynthetic activity the mutan...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02876678
更新日期：1978-01-01 00:00:00
abstract：:Inhibition of methanogenesis in ruminal cultures was attempted by hindering thiamine availability through its degradation by 'polyphenols' and competition for active sites on enzymes and transporters using thiamine structural analogs. Effects on fermentation were small and not consistently reversed by adding thiamine....
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-009-0075-9
更新日期：2009-11-01 00:00:00
abstract：:Resistance determinants for HgCl2 and CdCl2 were eliminated along with a number of antibiotic resistance factors from Acinetobacter lwoffi and Micrococcus sp. at 44 degrees C. These organisms were orginally resistant to HgCl2, merbromin, CdCl2, Pb(NO3)2, benzylpenicillin, erythromycin, carbenicillin, tetracycline and ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02925907
更新日期：1988-01-01 00:00:00
abstract：:The plasma membrane and mitochondria of bottom fermenting brewer's yeast obtained as a by-product of industrial beer production were isolated and the lipid fraction was analyzed. The phospholipid content accounted for 78 mg/g protein in the plasma membrane and 59 mg/g protein in the mitochondria. Major phospholipids i...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931290
更新日期：2005-01-01 00:00:00
abstract：:A total of 40 substances were tested for their inhibitory effect on the multiplication of a bacteriophage in a growing culture of Bacillus licheniformis and their influence on bacitracin production. Acriflavine was the only substance which, at a concentration of 3 microgram ml-1, completely suppressed phage multiplica...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02876597
更新日期：1978-01-01 00:00:00
abstract：:Among 18 tested strains of Claviceps spp., 7 produced significant amounts of exocellular polysaccharide (EPS). The maximum production of EPS was found in fermentation broth of Claviceps viridis. The kinetics of growth, substrate consumption, and EPS production in the batch, aerobic, submerged culture of this fungus we...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02993470
更新日期：2003-01-01 00:00:00
abstract：:Treatment of Helicobacter pylori cells with several chaotropic agents resulted in different degrees of inhibition in the binding of the bacteria to hemin and Congo-red dye. Polyanions also yielded a >50% inhibitory effect. Furthermore, hydrophobic interaction chromatography was used to determine the relative surface h...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-011-0043-z
更新日期：2011-05-01 00:00:00
abstract：:The submerged culture of the Claviceps purpurea strain studied was polymorphous. In the process of alkaloid synthesis, cytodifferentiation preceded biochemical differentiation. The onset of the alkaloid phase was characterized by: predominance of chlamydospores in the culture, the presence of vegetative cells with red...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02876766
更新日期：1975-01-01 00:00:00
abstract：:Several amino acids affected the amount of bacitracin produced by Bacillus licheniformis ATCC 10716 and the high-yielding mutant B. licheniformis AL. The possibility of an amino acid control mechanism for bacitracin biosynthesis is discussed. The two strains showed a different response to the addition of some amino ac...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02926454
更新日期：1979-01-01 00:00:00
abstract：:There is a growing interest in the use of bioinoculants to assist mineral fertilizers in improving crop production and yield. Azotobacter and Pseudomonas are two agriculturally relevant strains of bacteria which have been established as efficient bioinoculants. An experiment involving addition of graded concentrations...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-017-0495-x
更新日期：2017-05-01 00:00:00
abstract：:Genetic mapping of the chromosome of the bacteriophage PS8 of Agrobacterium tumefaciens using temperature sensitive (ts) mutants of the bacteriophage is described. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02927304
更新日期：1979-01-01 00:00:00
abstract：:Biochemical properties of a termostable alkaline phosphatase obtained from the mycelium extract of A. caespitosus were described. The enzyme was purified 42-fold with 32% recovery by DEAE-cellulose and concanavalin A-Sepharose chromatography. The molar mass estimated by Sephacryl S-200 or by 7% SDS-PAGE was 138 kDa an...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931303
更新日期：2007-01-01 00:00:00
abstract：:Antibacterial effect of chitooligosaccharides (COS) and low molar mass chitosans (LMWC) is considered as one of the most important characteristics of chitosan (CS) hydrolysates. Here, we show the in vitro effect of different COS, LMWC, and CS on representative anaerobic bacteria isolated from human colon as a possibil...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-012-0138-1
更新日期：2012-07-01 00:00:00
abstract：:A decrease in the content of ochratoxin A (OA) was observed in milk samples fermented by yoghurt bacteria and bifidobacteria. OA was added to the milk before fermentation at a rate of 0.05-1.5 mg/L. No residues of OA were found in samples containing 0.05 and 0.1 mg/L of OA, fermented by S. Salivarius subsp. thermophil...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02816335
更新日期：1996-01-01 00:00:00
abstract：:Microorganisms that cause chronic infections exist predominantly as surface-attached stable communities known as biofilms. Microbial cells in biofilms are highly resistant to conventional antibiotics and other forms of antimicrobial treatment; therefore, modern medicine tries to develop new drugs that exhibit anti-bio...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-018-0633-0
更新日期：2019-01-01 00:00:00
abstract：:Oxygen uptake by the spores of Fusarium moniliforme, F. oxysporum, F. semitectum, F. solani, Mucor racemosus and Trichoderma viride was increased in the presence of volatile substances extracted from Origanum majorana and Ocimum basilicum. This increase was greater in the presence of volatile substances from O. basili...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02885580
更新日期：1978-01-01 00:00:00
abstract：:Vibrio el tor cells contain a constitutive reductase enzyme which converts nitrofurantoin to an active principle that is responsible for the observed antibacterial activity of the drug. Acquisition of resistance of this strain towards nitrofurantoin is associated with the loss of this reductase. This enzyme is located...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02927181
更新日期：1979-01-01 00:00:00
abstract：:Viridans group streptococci bloodstream infections (VGS BSI) remain a significant cause of mortality and morbidity in patients with severe neutropenia. The goal of our study was to evaluate clinical course and microbiological susceptibility of VGS BSI at our center. Retrospective analysis of all microbiologically docu...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-017-0542-7
更新日期：2018-03-01 00:00:00
abstract：:Uropathogenic Escherichia coli (UPEC) isolates contain large genomic segments, termed pathogenicity islands (PAIs), that contribute to their virulence. A total of 150 UPEC and 50 commensal E. coli isolates from outpatients were investigated for antimicrobial susceptibility and the presence of eight PAI markers. One hu...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-015-0433-8
更新日期：2016-05-01 00:00:00
abstract：:Using the method of two separate UV exposures the increase of UV resistance after various induction fluences in growing and resting Escherichia coli B/r Hcr+ was followed. In resting cells, the optimum induction energy fluence was found to be 30 J/m2. In exponentially growing cells testing of induction has proved to b...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02926452
更新日期：1979-01-01 00:00:00
