Abstract：
:Microorganisms that cause chronic infections exist predominantly as surface-attached stable communities known as biofilms. Microbial cells in biofilms are highly resistant to conventional antibiotics and other forms of antimicrobial treatment; therefore, modern medicine tries to develop new drugs that exhibit anti-biofilm activity. We investigated the influence of a plant polyphenolic compound resveratrol (representative of the stilbene family) on the opportunistic pathogen Trichosporon cutaneum. Besides the influence on the planktonic cells of T. cutaneum, the ability to inhibit biofilm formation and to eradicate mature biofilm was studied. We have tested resveratrol as pure compound, as well as resveratrol in complex plant extract-the commercially available dietary supplement Regrapex-R-forte, which contains the extract of Vitis vinifera grape and extract of Polygonum cuspidatum root. Regrapex-R-forte is rich in stilbenes and other biologically active substances. Light microscopy imaging, confocal microscopy, and crystal violet staining were used to quantify and visualize the biofilm. The metabolic activity of biofilm-forming cells was studied by the tetrazolium salt assay. Amphotericin B had higher activity against planktonic cells; however, resveratrol and Regrapex-R-forte showed anti-biofilm effects, both in inhibition of biofilm formation and in the eradication of mature biofilm. The minimum biofilm eradicating concentration (MBEC80) for Regrapex-R-forte was found to be 2222 mg/L (in which resveratrol concentration is 200 mg/L). These methods demonstrated that Regrapex-R-forte can be employed as an anti-biofilm agent, as it has similar effect as amphotericin B (MBEC80 = 700 mg/L), which is routinely used in clinical practice.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Paldrychová M,Kolouchová I,Vaňková E,Maťátková O,Šmidrkal J,Krmela A,Schulzová V,Hajšlová J,Masák Jdoi
10.1007/s12223-018-0633-0subject
Has Abstractpub_date
2019-01-01 00:00:00pages
73-81issue
1eissn
0015-5632issn
1874-9356pii
10.1007/s12223-018-0633-0journal_volume
64pub_type
杂志文章abstract：:Two classes of newly synthesized amphiphilic compounds, phenolic antioxidants ("phenolics") and N-oxides exert in vivo antioxidant effects on live S. cerevisiae cells. Both groups have low toxicity, phenolics being more toxic than N-oxides and compounds with a longer alkyl chain having higher toxicity than those with ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02932187
更新日期：2007-01-01 00:00:00
abstract：:Killer strains of Saccharomyces cerevisiae producing killer toxin K1 kill sensitive cells but are resistant to their own toxin. It is assumed that in the producer, an effective interaction between the external toxin and its plasma membrane receptor or the final effector is not possible on the grounds of a conformation...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02932122
更新日期：2006-01-01 00:00:00
abstract：:The kinetics of extracellular neutral proteinase synthesis by an isogenic stringent (IS58) and a relaxed (IS56) strain of B. subtilis were compared. The specific enzyme formation rate by the stringent strain was higher than that of the relaxed one. Norvaline addition (1 mg/mL) induced the formation of pppGpp and ppGpp...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02883234
更新日期：1987-01-01 00:00:00
abstract：:The activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSP) as well as of succinate dehydrogenase (SDG), NADH dehydrogenase (NDG) and fumarate hydratase (FHT) were examined in relation to mitochondrial ultrastructure changes in Aspergillus niger exposed to N,N-bis(3-aminopropyl)dodecy...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931448
更新日期：2006-01-01 00:00:00
abstract：:Investigation of the dependence of cell yield of Candida boidinii in a chemostat and specific growth rate in a turbidostat on temperature was carried out using methanol as substrate. Each of the curves obtained had only one maximum, the optimum temperature for yield (Ys) and for specific growth rate (micron) being som...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02885600
更新日期：1977-01-01 00:00:00
abstract：:Virulence factors were compared in 15 Aeromonas spp. isolated from faeces of patients with Aeromonas-associated gastroenteritis and in 81 strains isolated from food. Strains from food did not show differences in the distribution of virulence factors when compared with strains isolated from faeces. However, 88.8% of Ae...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814323
更新日期：1994-01-01 00:00:00
abstract：:Antibody response to major porin proteins of S. Typhi (OmpC and OmpF) was evaluated in sera of typhoid patients (culture positive, n = 28; Widal positive, n = 16). Sera from fever patients (n = 6) having etiology other than Salmonella, and normal healthy human controls (n = 18) were also included. No significant diffe...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-010-0086-6
更新日期：2010-09-01 00:00:00
abstract：:Secreted aspartyl proteinase (Sap) distribution among different C. albicans isolates was determined using SAP-specific primers in polymerase chain reaction (PCR) assay. SAP1, SAP2, and SAP3 were detected in 13 of 40 (32.5%), SAP4 in 38/40 (95%), SAP5 were detected in 30/40 (75%), SAP6 in 23/40 (57.5%) of C. albicans s...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931422
更新日期：2005-01-01 00:00:00
abstract：:The biological activity and the presence of genes sfp and ituD (surfactin and iturin A) among Bacillus strains isolated from the Amazon basin were determined. Bacillus spp. were tested for hemolytic activity and inhibition of fungal growth by agar plate assays in parallel with PCR for identification of sfp and ituD ge...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-011-0056-7
更新日期：2011-07-01 00:00:00
abstract：:Vinasse, a recalcitrant waste of the ethanol industry was employed for the production of polyhydroxyalkanoate (PHA) by the extremely halophilic archaeon, Haloarcula marismortui in shake flasks. The PHA was recovered by osmotic lysis of the cells and subsequent purification by sodium hypochlorite and organic solvents. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-011-0092-3
更新日期：2012-01-01 00:00:00
abstract：:Procedures to introduce point mutations, restriction sites and insert or delete DNA fragments are very important tools to study protein function. We describe here two-step PCR-based method for generating single or multiple mutations, insertions and deletions in a small region of the sequence. In the first step, a uniq...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02816213
更新日期：1999-01-01 00:00:00
abstract：:Strains of fungi imperfecti of genera Aspergillus and Rhizopus were tested for the ability to produce alpha-mannosidases. The most suitable alpha-mannosidase producer of a total of 20 strains under study was Aspergillus Ravus Link 69. The parameters studied during the cultivation included the growth rate expressed as ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02876433
更新日期：1978-01-01 00:00:00
abstract：:The screening of 17 SAg genes of S. aureus isolated from the sputum of cystic fibrosis (CF) patients revealed that among 47 genetically different strains, 39 (83 %) carried SAg genes. Superantigens forming enterotoxin gene cluster were detected in 20 strains. The 2nd most common superantigen type was selk detected in ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931628
更新日期：2006-01-01 00:00:00
abstract：:The ability of the rumen ciliates to utilize β-glucans other than cellulose and xylan is currently being recognized. The objective of the present study was to characterize the ability of the ciliate Diploplastron affine to digest some pachyman, laminarin, pustulan, curdlan and lichean. The protozoa were isolated from ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-012-0120-y
更新日期：2012-07-01 00:00:00
abstract：:Mice (strains Balb/c and A/J) received an intravenous infusion of bilirubin for a 1 d period. The infusion was delivered at various phases of the primary reaction; the degree of the immune response was expressed as the number of antibody-forming cells against sheep erythrocytes. Bilirubin infusion during both the indu...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02897214
更新日期：1980-01-01 00:00:00
abstract：:Brefeldin A has been recently characterized to act as an inhibitor of intracellular protein export with profound effects on the structure and function of the Golgi apparatus in animal cells. Manifold activities of the antibiotic (under different names) published in the 1960's and 1970's are reviewed: effects on fungal...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/BF02814572
更新日期：1992-01-01 00:00:00
abstract：:Sixty four biopsy specimens of oral Candida leukoplakias were examined. Histological sections were stained with periodic acid-Schiff (PAS) reagent and by Grocott's silver method. C. albicans isolates were cultivated from all the patients but fungal hyphae were proved histologically only in 23 of them. PAS-positive and...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814437
更新日期：2001-01-01 00:00:00
abstract：:We report the complete nucleotide sequence and characterization of a small cryptic plasmid of Moraxella macacae 0408225, a newly described bacterial species within the family Moraxellaceae and a causative agent of epistaxis in macaques. The complete nucleotide sequence of the plasmid pMoma1 was determined and found to...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-014-0364-9
更新日期：2015-05-01 00:00:00
abstract：:A wild-type strain of Candida albicans (S1, ATCC 10261) was used to obtain stable auxotrophic colony morphological mutants (mutant M5 producing only true hyphae and mutant M2 containing 90 % blastospores and 10 % pseudohyphae) by induced mutagenesis. A hybrid was produced by somatic hybridization between these 2 mutan...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931528
更新日期：2004-01-01 00:00:00
abstract：:Overpopulation of domestic pigeons is considered to be one of the major problems of urban centers, as these birds are responsible for the dissemination of relevant pathogens to animal and human health. The aim of this study was to detect potentially pathogenic Escherichia coli and Salmonella spp. in domestic pigeons c...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-020-00781-w
更新日期：2020-08-01 00:00:00
abstract：:The production of bacterial enzymes in batch fermentations is compared with results obtained in continuous culture. When studying the production of alpha-amylase in Bacillus subtilis it was found that instability of the enzyme synthesis was due to nonhomogeneity of the population rather than to "the culture's history"...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02883137
更新日期：1982-01-01 00:00:00
abstract：:Isolation and structure elucidation was carried out of flavonoid constituents found in fractionated extracts of the seeds and green leaves of Daucus carota L. var. boissieri (Apiaceae). The flavonoids are mainly apigenin, luteolin, their glycosidic precursors and 2,4,5-trimethoxybenzaldehyde. Fatty acids, hydrocarbons...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02903720
更新日期：1999-01-01 00:00:00
abstract：:Verotoxin-producing Escherichia coli (VTEC) are nowadays among the most important emerging group of food-borne pathogens (VTEC strains cause gastroenteritis that can be complicated by the hemorrhagic colitis or hemolytic uremic syndrome, HUS). Escherichia coli 026 producing verotoxin 2 was isolated and its identity co...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931454
更新日期：2005-01-01 00:00:00
abstract：:The present paper reports a modified method for isolation of lysostaphin--a bacteriolytic agent with specific affinity for staphylococcal cell wall. The proposed purification scheme includes three steps. The first procedure is ultrafiltration through a membrane filter giving a yield of 75.6%. The result of ultrafiltra...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814386
更新日期：1993-01-01 00:00:00
abstract：:Effect of oxygen and metabolic substrates (glucose, ethanol) on the catalase activity of anaerobically grown Schizosaccharomyces pombe cells was assessed and compared with that of Saccharomyces cerevisiae in order to determine the catalase activity regulation in S. pombe. In contrast to S. cerevisiae, the total catala...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02818575
更新日期：1998-01-01 00:00:00
abstract：:Biotechnological conversion of low-cost agro-industrial by-products, such as industrial waste or terpenes from the distillation of essential oils from plants into more valuable oxygenated derivatives, can be achieved by using microbial cells or enzymes. In Argentina, the essential oil industry produces several tons of...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-015-0422-y
更新日期：2016-03-01 00:00:00
abstract：:The effect of the newly synthesized phosphonic compound dibutyl 2-octylamino-2-propanephosphonate (DBOP) on the growth of the aquatic plant Spirodela oligorrhiza and stability of red blood cells (RBC) and planar lipid membranes (BLM) was studied to determine its physiological activity and, if possible, correlate this ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02908944
更新日期：2000-01-01 00:00:00
abstract：:In ostriches and pheasants, there is still limited information relating to staphylococci and their properties. Biogenic amines (BAs) are nitrogenous low-molecular-weight substances with biological functions in animals, plants and microorganisms. In this study, we focused on BA production by targeted faecal staphylococ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-017-0492-0
更新日期：2017-05-01 00:00:00
abstract：:Adhesion of group B streptococci to human epithelial vaginal and buccal cells proceeded in three phases which differed qualitatively. Maximum adhesion took place within 10 min of interaction, during the second phase (10-15 min), the percentage of adherent cells decreased significantly (P less than 0.05) whereas during...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02884088
更新日期：1983-01-01 00:00:00
abstract：:Euglena ignobilis cells in natural puddle water of pH 7.8, when kept at 21 +/- 2 degrees C and under continuous light (intensity of approximately 30 micromol m(-2) s(-1)) in a culture chamber, decreased their speed of movement from > or = 78000 microm/min (after a 12-h cultivation), to 850-1300 microm/min after 18 h. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931412
更新日期：2005-01-01 00:00:00
