Abstract:
:Killer strains of Saccharomyces cerevisiae producing killer toxin K1 kill sensitive cells but are resistant to their own toxin. It is assumed that in the producer, an effective interaction between the external toxin and its plasma membrane receptor or the final effector is not possible on the grounds of a conformation change of the receptor or its absence in a membrane. Therefore, it is possible that some mutants with defects in intracellular protein transport and degradation can show a suicidal phenotype during K1 toxin production. We have examined these mutants in a collection of S. cerevisiae strains with deletions in various genes transformed by the pYX213+M1 vector carrying cDNA coding for the K1 toxin under the control of the GAL1 promoter. Determination of the quantity of dead cells in colony population showed that (1) the toxin production from the vector did not support full immunity of producing cells, (2) the suicidal phenotype was not connected with a defect in endocytosis or autophagy, (3) deletants in genes VPS1, VPS23, VPS51 and VAC8 required for the protein degradation pathway between the Golgi body and the vacuole exhibited the highest mortality. These results suggest that interacting molecule(s) on the plasma membrane in the producer might be diverted from the secretion pathway to degradation in the vacuole.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Valis K,Masek T,Novotná D,Pospísek M,Janderová Bdoi
10.1007/BF02932122subject
Has Abstractpub_date
2006-01-01 00:00:00pages
196-202issue
3eissn
0015-5632issn
1874-9356journal_volume
51pub_type
杂志文章abstract::The effect of paraquat on the activities of antioxidant defense and detoxifying enzymes of the white-rot fungus Funalia trogii was determined. Paraquat increased the activities of glutathione reductase (GR), glutathione transferase (GT) and superoxide dismutase at 1 mmol/L, while at 0.1 mmol/L it did not affect the ac...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02818700
更新日期:2002-01-01 00:00:00
abstract::The aim of this study was to evaluate the contribution of plasmid-mediated genes and efflux to fluoroquinolone resistance in collection of Achromobacter spp. gathered during a 3-year period. Susceptibility to ciprofloxacin and levofloxacin was tested by disk diffusion and microdilution tests for a collection of 98 Ach...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-018-0639-7
更新日期:2019-03-01 00:00:00
abstract::Small plasmid pKST23 was isolated from sheep ruminal Escherichia coli population. Plasmid sequence was determined to be 2,779 bp in length and was found to have an overall 42 % of GC pairs. However, its sequence can be divided into two regions based on genetic composition and the GC content. It was found that the high...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-012-0124-7
更新日期:2012-07-01 00:00:00
abstract::Isolates of Stenotrophomonas maltophilia species display the feature "temperature-dependent susceptibility" (TDS) to antibiotics. Both 30TDS strains (at least 4 times lower value of minimum inhibitory concentration (MIC) of an antibiotic at 30 than at 37 degrees C) and 37TDS strains (at least 4 times lower value of MI...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02818682
更新日期:2002-01-01 00:00:00
abstract::Mineral nutrition of crop plants is one of the major challenges faced by modern agriculture, particularly in arid and semi-arid regions. In alkaline calcareous soils, the availability of phosphorus and zinc is critically less due to their fixation and precipitation as complexes. Farmers use fertilizers to fulfill crop...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-020-00831-3
更新日期:2020-10-24 00:00:00
abstract::A quarantine organism, "Candidatus Phytoplasma mali," is the causal agent of apple proliferation, one of the most important apple diseases in Europe. The genetic diversity of this pathogen in Central and Southern Europe has already been reported; however, almost no data exists from Eastern Europe. In this study, "Ca. ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-018-0660-x
更新日期:2019-05-01 00:00:00
abstract::The level of an antibiotic capable of inhibiting the etiological agent at the site of infection is an essential prerequisite for successful antibiotic therapy. In some cases, locally applied antibiotics may compensate for limitations of systemic administration and shorten systemic therapy. We aimed at verifying to wha...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-011-0093-2
更新日期:2012-09-01 00:00:00
abstract::Physiological and biochemical properties were tested in 45 isolates of Aspergillus egyptiacus (16 isolates), Emericella nidulans (16) and Aspergillus versicolor (13). The three fungal species exhibited common and similar features. The big similarity between A. egyptiacus and E. nidulans was greater than between A. egy...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02814449
更新日期:1994-01-01 00:00:00
abstract::This is the first report describing precipitation of bacteriophage particles with zinc chloride as a method of choice to isolate infectious lytic bacteriophages against Pectobacterium spp. and Dickeya spp. from environmental samples. The isolated bacteriophages are ready to use to study various (ecological) aspects of...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-015-0411-1
更新日期:2016-01-01 00:00:00
abstract::The resistance to killing by free radicals of two mutants ofPhaffia rhodozyma was determined. Mutant 5-7 did not produce astaxanthin but produced beta-carotene, while mutant 3-4 did not produce any carotenoid pigments. The resistance of mutant 5-7 was the same as that of the wild type but mutant 3-4 was rapidly killed...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02816505
更新日期:1998-01-01 00:00:00
abstract::The gene encoding the coat protein (CP) of a potato virus Y (PVY) was cloned into expression vector pMPM-A4Omega. PVY CP was expressed in Escherichia coli and the purified recombinant protein was used for raising rabbit polyclonal antibodies. The sera and antibodies were tested for the detection of PVY in the laborato...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-008-0067-1
更新日期:2008-01-01 00:00:00
abstract::A high infective dose of Taxocara canis eggs (2,500 eggs per mouse) induced a partial immunosuppression in mice, manifested by inhibition of the proliferative response of splenic T and B cells to polyclonal activators. A glucan immunomodulator given to infected animals at the beginning of the experiment showed a marke...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02820794
更新日期:1998-01-01 00:00:00
abstract::Fourteen of 200 Lactobacillus isolates from African fermented foods, viz. 'wara', 'kenkey', 'ugba', 'ogi', 'kunuzarki', 'fufu' and 'iru' were found to produce bacteriocins against L. plantarum and only three bacteriocinogenic isolates inhibited some of the food pathogens. Plasmid analysis of the 14 bacteriocin-produci...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02814645
更新日期:1994-01-01 00:00:00
abstract::Ultrastructural changes induced by Rickettsia slovaca standard type (ST) and wild type (WT) were examined during their life cycle in L929 and Vero cells. R. slovaca invaded the cytoplasm of the host cell by phagocytosis on the 1st d p.i. Rickettsiae adhering to the cytoplasmic membrane were engulfed by cellular extens...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-009-0019-4
更新日期:2009-01-01 00:00:00
abstract::Differences in the effect of volatile and gaseous metabolites of germinating pea seeds on the germination of spores of Mucor racemosus and macroconidia of Fusarium oxysporum are described. Germination of spores of M. racemosus was inhibited by seed metabolites whereas germination of macroconidia of F. oxysporum was st...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02933019
更新日期:1980-01-01 00:00:00
abstract::During cultivation in a minimal medium with glucose Alternaria tenuissima and Aspergillus vesicolor produce constitutively alpha- and beta-glucanases. Fractions of beta-1,3-glucanases exhibiting affinity for laminarin were separated by means of gel filtration chromatography. Two neutral beta-1,3-glucanases with affini...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02876394
更新日期:1980-01-01 00:00:00
abstract::Oxygen uptake by the spores of Fusarium moniliforme, F. oxysporum, F. semitectum, F. solani, Mucor racemosus and Trichoderma viride was increased in the presence of volatile substances extracted from Origanum majorana and Ocimum basilicum. This increase was greater in the presence of volatile substances from O. basili...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02885580
更新日期:1978-01-01 00:00:00
abstract::The microbial fuel cells (MFCs) are recognized to be highly effective for the biodegradation of phenol. For isolating the phenol-degrading bacteria, the sample containing 500 mg/L phenol was collected from the MFCs. The strain (WL027) was identified basing on the 16S rRNA gene analysis and phylogenetic analysis as Bac...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-017-0536-5
更新日期:2018-01-01 00:00:00
abstract::Secreted aspartyl proteinase (Sap) distribution among different C. albicans isolates was determined using SAP-specific primers in polymerase chain reaction (PCR) assay. SAP1, SAP2, and SAP3 were detected in 13 of 40 (32.5%), SAP4 in 38/40 (95%), SAP5 were detected in 30/40 (75%), SAP6 in 23/40 (57.5%) of C. albicans s...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931422
更新日期:2005-01-01 00:00:00
abstract::Aliphatic 1,2-alkanolamines inhibited beta-glucanase from Candida utilis. The highest inhibitory effect was observed with 1-dodecylamino-3-chloro-2-propanol (Ki = 1.0 mumol/L) and it was much higher than that of D-glucono-delta-lactone. Simple 1,2-alkanolamines, like 2-aminoethanol or 2-methylaminoethanol, did not exh...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02898763
更新日期:1993-01-01 00:00:00
abstract::Plenty of metagenomic studies have suggested possible associations between microbiome composition and colorectal cancer (CRC). However, these techniques are not economic enough for routine use so far. Therefore, we explored the possibility to detect species associated with colorectal cancer by conventional culture fro...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-020-00807-3
更新日期:2020-12-01 00:00:00
abstract::A selection of tert-butylhydroperoxide (tBOOH)-tolerant Candida albicans mutants showed increased tolerances to 19 different stress conditions. These mutants are characterized by a constitutively upregulated antioxidative defense system and, therefore, adaptation to oxidative stress may play an important role in gaini...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-014-0305-7
更新日期:2014-07-01 00:00:00
abstract::It is proposed that the transcription of the c-2 repressor gene initiates at two promoters, pre and prm, similarly to the transcription of the c-I gene of lambda phage. In the P22 phage, these promoters are defined by mutations of c54 and K5. ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02876780
更新日期:1975-01-01 00:00:00
abstract::NaN3 was found to inhibit transformation but not the irreversible binding of donor 3H-DNA in competent cells of the original low-transformable strain Bacillus subtilis 168 trp2. Addition of NaN3 to cells of two mutants Bacillus subtilis HT39 and HT46 with an increased transformability decreased substantially the irrev...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02877669
更新日期:1977-01-01 00:00:00
abstract::The treatment of three patients suffering from chronic bacterial prostatitis who were qualified for an experimental phage therapy protocol managed at the Phage Therapy Unit in Wrocław is described. They had previously been treated unsuccessfully with long-term targeted antibiotics, autovaccines, and laser biostimulati...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-009-0064-z
更新日期:2009-09-01 00:00:00
abstract::Three methods were used for the isolation of fungi in the present investigation: the dilution-plate method, hair-baiting technique with horse hairs and the "exposed plate" method. Sabouraud's glucose agar at 28 degrees C was also used as isolation medium. 102 species and 2 species varieties belonging to 36 genera were...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02821282
更新日期:1990-01-01 00:00:00
abstract::The majority of antibiotics and substances with diverse biological activity used in medicine are produced by actinomycetes, nonfilamentous bacteria and fungi. Other microorganisms, such as myxobacteria, pseudomonads, nocardias, basidiomycetes, marine microorganisms, enterobacteria, halobacteria, hyperthermophiles etc....
journal_title:Folia microbiologica
pub_type: 杂志文章,评审
doi:10.1007/BF02993460
更新日期:2003-01-01 00:00:00
abstract::Fifty Escherichia coli strains isolated from stool samples of 51 healthy children, 143 strains isolated from stool samples of 327 children with diarrhea and 24 strains isolated from stool samples of 21 children with suspected hemolytic uremic syndrome were examined for the presence of Shiga toxin-producing E. coli vir...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02817680
更新日期:2002-01-01 00:00:00
abstract::Staphylococcus epidermidis is a biofilm-forming bacterial strain that can cause major problems as an agent of nosocomial infections. Bacteria in biofilms are shielded from the environment and can survive high doses of antibiotics. We here test the antibiotic susceptibility of Staphylococcus epidermidis to rising genta...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-017-0568-x
更新日期:2018-05-01 00:00:00
abstract::Distribution of microtubules and F-actin in aerobically growing cells of Dipodascus magnusii, belonging to the class Saccharomycetes was analyzed using immunofluorescence microscopy and labeling with rhodamine-tagged phalloidin. A conspicuous system of permanent cytoplasmic microtubules was observed in association wit...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02930952
更新日期:2003-01-01 00:00:00