Abstract:
:Small plasmid pKST23 was isolated from sheep ruminal Escherichia coli population. Plasmid sequence was determined to be 2,779 bp in length and was found to have an overall 42 % of GC pairs. However, its sequence can be divided into two regions based on genetic composition and the GC content. It was found that the high GC region spanning approximately from nucleotide 1,300 to 2,750 was identical to a group of small Escherichia coli plasmids and encoded a putative replication protein identical to plasmid pKL1 Rep protein. The part with lower GC pairs seemed to be more specific as it showed no similarity to the GenBank database. Computational analysis revealed four open reading frames, two of which showed considerable homology to replication proteins. PCR primers targeting parts of the two different regions of plasmid pKST23 were used to assess the occurrence of related plasmids within ruminal E. coli population.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Fecskeová L,Kovařík M,Javorský P,Pristaš Pdoi
10.1007/s12223-012-0124-7subject
Has Abstractpub_date
2012-07-01 00:00:00pages
277-9issue
4eissn
0015-5632issn
1874-9356journal_volume
57pub_type
杂志文章abstract::Mineral nutrition of crop plants is one of the major challenges faced by modern agriculture, particularly in arid and semi-arid regions. In alkaline calcareous soils, the availability of phosphorus and zinc is critically less due to their fixation and precipitation as complexes. Farmers use fertilizers to fulfill crop...
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journal_title:Folia microbiologica
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journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02817448
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journal_title:Folia microbiologica
pub_type: 杂志文章,评审
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doi:10.1007/BF02931279
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abstract::Vancomycin is often used in orthopedic surgery as a local prophylaxis of bacterial infection. The aim of this work was to compare the release of vancomycin and its biologically inactive crystalline degradation products (CDP-1) during in vitro experiments from different types of local antibiotic delivery systems (bone ...
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pub_type: 杂志文章
doi:10.1007/s12223-019-00752-w
更新日期:2020-06-01 00:00:00
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doi:10.1007/s12223-010-0004-y
更新日期:2010-01-01 00:00:00
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journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-015-0408-9
更新日期:2016-01-01 00:00:00
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doi:10.1007/s12223-011-0056-7
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journal_title:Folia microbiologica
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doi:10.1007/BF02927251
更新日期:1981-01-01 00:00:00
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journal_title:Folia microbiologica
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journal_title:Folia microbiologica
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