Abstract：
:Enterococcus faecalis is a ubiquitous bacterium of the gut that is observed in persistent periradicular infections. Its pathogenicity is associated with biofilm formation and the ability to survive under nutrient-poor (starvation) conditions. However, characteristics of chemical composition of biofilm cells developed by starved E. faecalis cells remain poorly understood. In this study, E. faecalis cells in exponential, stationary, and starvation phases were prepared and separately cultured to form biofilms. Confocal laser scanning microscopy was performed to verify biofilm formation. Raman microscopy was used to investigate the chemical composition of cells within the biofilms. Compared to cells in exponential or stationary phase, starved cells developed biofilms with fewer culturable cells (P < 0.05). Raman analysis revealed that cells produced in the biofilms from starved planktonic cells contained more protein and less nucleic acids than either the corresponding planktonic cells or the cells in biofilms from planktonic cells in exponential or stationary phases, suggesting that biofilm-grown cells from the starvation phase were characterized by increased synthesis of proteins and decreased nucleic acids. This study provides an insight into the chemical composition of biofilm cells developed by starved E. faecalis.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Liu H,Xu Q,Huo L,Wei X,Ling Jdoi
10.1007/s12223-014-0319-1subject
Has Abstractpub_date
2014-09-01 00:00:00pages
447-53issue
5eissn
0015-5632issn
1874-9356journal_volume
59pub_type
杂志文章abstract：:A collection of 34 lactococcal strains were characterized using the polymerase chain reaction (PCR) for the acmA gene, and for the 16S rDNA gene, and DNA fingerprinting methods for randomly amplified polymorphic DNA (RAPD) and repetitive extragenic palindrome-PCR (rep-PCR). PCR experiments corroborated the genotypic i...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931464
更新日期：2005-01-01 00:00:00
abstract：:Isolation and structure elucidation was carried out of flavonoid constituents found in fractionated extracts of the seeds and green leaves of Daucus carota L. var. boissieri (Apiaceae). The flavonoids are mainly apigenin, luteolin, their glycosidic precursors and 2,4,5-trimethoxybenzaldehyde. Fatty acids, hydrocarbons...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02903720
更新日期：1999-01-01 00:00:00
abstract：:Mutations occurring in viral polymerase gene of hepatitis B virus (HBV) due to the use of nucleos(t)id analogs reduce the activity of the drugs by causing antiviral resistance. In this study, it was aimed to evaluate mutations responsible for drug resistance and drug resistance mutation rates in patients followed up b...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-018-0650-z
更新日期：2019-03-01 00:00:00
abstract：:A decrease in the content of ochratoxin A (OA) was observed in milk samples fermented by yoghurt bacteria and bifidobacteria. OA was added to the milk before fermentation at a rate of 0.05-1.5 mg/L. No residues of OA were found in samples containing 0.05 and 0.1 mg/L of OA, fermented by S. Salivarius subsp. thermophil...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02816335
更新日期：1996-01-01 00:00:00
abstract：:The mean lengths of G1, S, G2 and M phases of the cell cycle were determined on the basis of the population distribution of Candida utilis grown in a continuous culture under steady-state conditions by using an original mathematical method. The length of the G2 phase was proportional to that of G1; the length of M was...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02821281
更新日期：1990-01-01 00:00:00
abstract：:As a part of a program to develop starter cultures aiding in the spoilage control and sanitation of African fermented foods, a cereal-based food ('ogi' and its solid form 'agidi' or 'eko') was prepared using a bacteriocin-producing Lactobacillus strain as the starter culture. The survival of an enterotoxigenic Escheri...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02898642
更新日期：1997-01-01 00:00:00
abstract：:Asymptomatic uropathogenic Escherichia coli (UPECs) are the leading cause of asymptomatic bacteriuria (ABU) in humans. So this study aimed to identify and characterize ABU UPECs from hospitalized patients of Kolkata, India, with respect to their antibiogram profile, phylogeny, pathogenicity islands, and virulence fact...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-019-00685-4
更新日期：2019-07-01 00:00:00
abstract：:The gene encoding the coat protein (CP) of a potato virus Y (PVY) was cloned into expression vector pMPM-A4Omega. PVY CP was expressed in Escherichia coli and the purified recombinant protein was used for raising rabbit polyclonal antibodies. The sera and antibodies were tested for the detection of PVY in the laborato...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-008-0067-1
更新日期：2008-01-01 00:00:00
abstract：:The rosettes formed by mouse peritoneal macrophages or DCH-5 cells and TNP-erythrocytes coated with anti-TNP antibodies of different isotypes were inhibited to various extent by monosaccharides. The most effective inhibitors were N-acetylglucosamine, glucosamine, mannose and N-acetylneuraminic acid in 1-5 mmol/L conce...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814584
更新日期：1992-01-01 00:00:00
abstract：:The submerged culture of the Claviceps purpurea strain studied was polymorphous. In the process of alkaloid synthesis, cytodifferentiation preceded biochemical differentiation. The onset of the alkaloid phase was characterized by: predominance of chlamydospores in the culture, the presence of vegetative cells with red...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02876766
更新日期：1975-01-01 00:00:00
abstract：:Ovine and caprine scrapie occupies a unique place among animal transmissive spongiform encephalopathies (TSE). It is an object of intensive biomedicinal, ecological and economical studies. Its causative agents are demonstrably associated with the development of TSE in farmed minks, goats and moufflons. Ovine strains o...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/BF02818713
更新日期：2000-01-01 00:00:00
abstract：:A case report of ventriculoperitoneal shunt infection caused by Candida lusitaniae in a 6-year-old patient with cerebral astrocytoma and obstructive hydrocephalus is presented briefly with emphasis on the course of antifungal treatment. Seven isolates recovered subsequently from the cerebrospinal fluid were studied re...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-011-0063-8
更新日期：2011-09-01 00:00:00
abstract：:The aim of this study was to evaluate the contribution of plasmid-mediated genes and efflux to fluoroquinolone resistance in collection of Achromobacter spp. gathered during a 3-year period. Susceptibility to ciprofloxacin and levofloxacin was tested by disk diffusion and microdilution tests for a collection of 98 Ach...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-018-0639-7
更新日期：2019-03-01 00:00:00
abstract：:Cryptosporidiosis belongs to the important parasitic infections with zoonotic potential and the occurrence in European countries is rare. The first cases of cryptosporidiosis caused by Cryptosporidium hominis detected in the Slovak republic were described here. Collection of examined humans consisted of five family me...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-012-0182-x
更新日期：2013-01-01 00:00:00
abstract：:Biochemical properties of a termostable alkaline phosphatase obtained from the mycelium extract of A. caespitosus were described. The enzyme was purified 42-fold with 32% recovery by DEAE-cellulose and concanavalin A-Sepharose chromatography. The molar mass estimated by Sephacryl S-200 or by 7% SDS-PAGE was 138 kDa an...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931303
更新日期：2007-01-01 00:00:00
abstract：:A new metabolite was isolated from the culture of Penicillium rugulosum and its structure was determined from physico-chemical data. Accompanying metabolites skyrin and rugulosin were characterized by UV, IR, CD, mass and NMR spectra. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02876598
更新日期：1978-01-01 00:00:00
abstract：:The kinetics of extracellular neutral proteinase synthesis by an isogenic stringent (IS58) and a relaxed (IS56) strain of B. subtilis were compared. The specific enzyme formation rate by the stringent strain was higher than that of the relaxed one. Norvaline addition (1 mg/mL) induced the formation of pppGpp and ppGpp...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02883234
更新日期：1987-01-01 00:00:00
abstract：:The aim of this study was to determine the prevalence of Listeria sp. and Listeria monocytogenes in soil samples with reference to type of fertilizers (natural and artificial) and distance from places intensively exploited by men, as well as to determine the relationship between the presence of L. monocytogenes in the...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-013-0260-8
更新日期：2014-01-01 00:00:00
abstract：:Biofilms represent mixed communities present in a diverse range of environments; however, their utility as inoculants is less investigated. Our investigation was aimed towards in vitro development of biofilms using fungal mycelia (Trichoderma viride) as matrices and nitrogen-fixing and P-solubilizing bacteria as partn...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-012-0154-1
更新日期：2012-09-01 00:00:00
abstract：:Two classes of newly synthesized amphiphilic compounds, phenolic antioxidants ("phenolics") and N-oxides exert in vivo antioxidant effects on live S. cerevisiae cells. Both groups have low toxicity, phenolics being more toxic than N-oxides and compounds with a longer alkyl chain having higher toxicity than those with ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02932187
更新日期：2007-01-01 00:00:00
abstract：:Streptomyces aureofaciens glucosidizes 1,2,4-trihydroxy-9,10-anthraquinone (purpurin) added to the cultivation medium to yield the corresponding 2-beta-D-glucoside. The identity of the glucoside was demonstrated by comparing its physico-chemical properties with data of an authentic sample prepared synthetically. A fur...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02873156
更新日期：1984-01-01 00:00:00
abstract：:The prevalence of extended-spectrum beta-lactamases (ESBL) was determined among isolates of Escherichia coli (n = 63) isolated from hospitalized (43) and healthy (20) children. Ten isolates (21%) were ESBL-positive for two screening tests, the double disk-synergy test and the Oxoid Combination Disk method. One ESBL-po...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02930963
更新日期：2003-01-01 00:00:00
abstract：:A wild-type strain of Candida albicans (S1, ATCC 10261) was used to obtain stable auxotrophic colony morphological mutants (mutant M5 producing only true hyphae and mutant M2 containing 90 % blastospores and 10 % pseudohyphae) by induced mutagenesis. A hybrid was produced by somatic hybridization between these 2 mutan...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931528
更新日期：2004-01-01 00:00:00
abstract：:Manganese peroxidase (MnP) was produced by shallow stationary cultures of Phanerochaete chrysosporium growing on N-limited medium. Decolorization of sulfonphthalein (SP) dyes by MnP was investigated. The MnP activity profile and decolorization of SP dyes was correlated and almost all dyes were decolorized at pH 4.0. T...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931512
更新日期：2003-01-01 00:00:00
abstract：:The root nodules of Phaseolus mungo (a herbaceous leguminous pulse) contained a high amount of 3-indolylacetic acid (IAA). A tryptophan pool present in the nodule might play the role of precursor for IAA production. From the root nodule a Rhizobium sp. was isolated. The symbiont produced a large amount of IAA (142 mic...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-008-0054-6
更新日期：2008-01-01 00:00:00
abstract：:Using fluorescein-labelled antibodies against gamma, mu and alpha chains, Ig-containing cells in palatine tonsils were studied in 120 patients. The aim of this study was to determine the most frequently repeated typical findings as regards the numbers and localisation of these cells in tonsils and to confront the data...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02927432
更新日期：1981-01-01 00:00:00
abstract：:Staphylococcus aureus is a leading cause of bloodstream infections (BSI) and diseases that may be caused by hematogenous spread. The staphylococcal adhesin, for which the association with the infections emerging as a complication of septicemia has been well documented, is a bone sialoprotein-binding protein (Bbp). The...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-014-0321-7
更新日期：2014-11-01 00:00:00
abstract：:One-week dextran sulfate treatment of conventional (CV) immunodeficient (SCID) mice gave rise to acute colitis in the colon mucosa; germ-free (GF) SCID mice did not exhibit any changes in colon morphology. Dextran sulfate application to CV immunocompetent (BALB/c) mice did induce substantial changes in the colon mucos...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02818004
更新日期：2001-01-01 00:00:00
abstract：:Altered cell signaling is the molecular basis for cell proliferation occurring in association with several gamma herpesvirus infections. Three gamma herpesviruses, namely EBV/HHV-4, KSHV/HHV-8 and the MHV-68 (and/or MHV-72) and their unusual cell-pirated gene products are discussed in this respect. The EBV, KSHV as we...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/BF02931360
更新日期：2003-01-01 00:00:00
abstract：:White-rot fungi, Coriolus versicolor and Funalia trogii, produced laccase on media with diluted olive-oil mill wastewater and vinasse. Addition of spent cotton stalks enhanced the laccase activity with a maximum after 12 d of cultivation. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02873591
更新日期：2001-01-01 00:00:00
