Use of nalidixic acid for constructing the replication map of the Mycobacterium phlei chromosome.

Abstract:

:Nalidixic acid was used for describing more accurately the terminal replication region of the Mycobacterium phlei chromosome. Cell division in synchronized cultures was not sensitive to this acid any more between 185-190 min, i.e. about 10 min after replication of the ser gene, the last of 24 genes of the replication map described so far. The replication of the chromosome was controlled by determining the position of the bac gene. Microscopic studies in phase contrast of the cells that were subjected for long time periods to nalidixic acid treatment at a bactericidal concentration showed elongated cells. The electronmicroscopic observation showed that a portion of the population influenced by nalidixic acid lyses, whereas other cells remain intact and resemble control cells.

journal_title

Folia microbiologica

authors

Konícek J,Konícková-Radochová M,Cáslavská J,Wolf A

doi

10.1007/BF02927251

subject

Has Abstract

pub_date

1981-01-01 00:00:00

pages

277-82

issue

4

eissn

0015-5632

issn

1874-9356

journal_volume

26

pub_type

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