Abstract:
:Vancomycin is often used in orthopedic surgery as a local prophylaxis of bacterial infection. The aim of this work was to compare the release of vancomycin and its biologically inactive crystalline degradation products (CDP-1) during in vitro experiments from different types of local antibiotic delivery systems (bone grafts and bone cements). The concentrations of vancomycin and its crystalline degradation products were determined by high-performance liquid chromatography. Each experiment was performed in a phosphate buffer solution over 21 days. Morselized bone grafts, synthetic bone cements Palacos and Copal, and synthetic bone grafts were tested as local carriers of vancomycin. The highest concentration approximately 670 mg/L of vancomycin was released from synthetic bone grafts Actifuse. Even after 21 days, the concentration of vancomycin was still above the minimum inhibitory concentration (MIC). The maximum concentration of vancomycin released in two experiments with human bone grafts exceeded 600 mg/L during the first day and was still above MIC level 21 days later when the experiment was concluded. By comparing the synthetic bone cements Palacos and Copal, Copal had the average maximum concentration of only 32.4 mg/L and Palacos 35.7 mg/L. The concentration of vancomycin fell below the MIC for vancomycin-resistant Staphylococcus aureus (VRSA) on the seventh day with Palacos and the ninth day with Copal. This study showed the insufficient concentration of released vancomycin from synthetic bone cements at the end of the experiment. For improvement of local prophylaxis, it would be beneficial to increase the amount of vancomycin in bone cements.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Klapkova E,Nescakova M,Melichercik P,Jahoda D,Dunovska K,Cepova J,Prusa Rdoi
10.1007/s12223-019-00752-wsubject
Has Abstractpub_date
2020-06-01 00:00:00pages
475-482issue
3eissn
0015-5632issn
1874-9356pii
10.1007/s12223-019-00752-wjournal_volume
65pub_type
杂志文章abstract::Some Bacillus subtilis mutants with different levels of homoserine dehydrogenase were described. Strains that do not accumulate methionine have a high homoserine dehydrogenase activity. Low activity was detected in mutants where cell growth was completely inhibited by 0.7 mmol/L methionine. A low concentration of dime...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02884064
更新日期:1991-01-01 00:00:00
abstract::Analysis of actA gene sequence polymorphism has been shown to be an effective and relatively inexpensive method for subtyping Listeria monocytogenes isolates, allowing the division of the population of this species into two deeply separate lineages. This sequence-based method as well as PCR-mediated fingerprinting wer...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-009-0003-z
更新日期:2009-01-01 00:00:00
abstract::Using phenol--water extraction, a lipopeptidopolysaccharide complex (LPPS) was isolated from Listeria monocytogenes. Some biological and immunological properties of LPPS were compared with lipopolysaccharide isolated by the same procedure from Salmonella typhimurium. LPPS possesses low pyrogenicity, but the immunologi...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02923515
更新日期:1985-01-01 00:00:00
abstract::Oxidizing and assimilating ability of the yeast Candida utilis 49 was tested with 21 different low-boiling organic compounds which come as components of raw synthetic ethanol. The highest yields of yeast dry weight were obtained with ethanol (72.0%), propanol (48.2%), ethyl acetate (43.4%) and acetic acid (34.2%). To ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02876965
更新日期:1976-01-01 00:00:00
abstract::Using mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine, a number of homoserine auxotrophs have been isolated from a glutamate-producing Arthrobacter globiformis excreting L-lysine in good amounts. For further improvement, mutants resistant to the lysine analog S-(2-aminoethyl)-L-cysteine have been isolated from h...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02935823
更新日期:1991-01-01 00:00:00
abstract::Blastoschizomyces capitatus is an uncommon, opportunistic pathogenic fungus, which causes invasive and disseminated infections. This microorganism is normally present in both environmental and normal human flora. Within a host, B. capitatus is able to grow in both unicellular yeast and multicellular filamentous growth...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-013-0273-3
更新日期:2014-03-01 00:00:00
abstract::Mice (strains Balb/c and A/J) received an intravenous infusion of bilirubin for a 1 d period. The infusion was delivered at various phases of the primary reaction; the degree of the immune response was expressed as the number of antibody-forming cells against sheep erythrocytes. Bilirubin infusion during both the indu...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02897214
更新日期:1980-01-01 00:00:00
abstract::A total of 152 lactic acid bacteria (LAB) were isolated from pickles produced in the Ankara-Çubuk region. These isolates were clustered into eight groups on the basis of their phenotypic characteristics including cell morphology, CO2 production from glucose, growth at 10 and 45 °C, growth in 6.5 % NaCl, and growth at ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-014-0363-x
更新日期:2015-05-01 00:00:00
abstract::Spatial distribution of ectomycorrhizae-associated basidiomycetes was determined in oakbirch forest using terminal restriction fragment length polymorphism (T-RFLP) analysis. The data were correlated with actual soil humidity, pH, electric conductivity of the soil extract, absorbance A(465) and A(665) of water and alk...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-010-0076-8
更新日期:2010-09-01 00:00:00
abstract::In this study, a method combining Raman spectroscopy with chemometric analysis was developed for detection of phage presence in raw milk and discrimination of Streptococcus thermophilus and Lactobacillus bulgaricus phages which are among the main phages causing problems in dairy industry. For this purpose, S. thermoph...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-018-0604-5
更新日期:2018-09-01 00:00:00
abstract::Sporulation was repressed in the parent strain by various carbon sources whereas glucose-resistant mutants were resistant to them but not to glycerol 2-phosphate. Both mycobacillin and dipicolinic acid synthesis were repressed in the parent by some of the compounds tested, viz. glucose, pyruvate and glycerol 2-phospha...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02928000
更新日期:1986-01-01 00:00:00
abstract::Single chain Fv (scFv) antibodies (generated by phage display technology, molecules representing new and efficient tools in the research and diagnostics of infectious diseases) against the capsid protein (p25) of Maedi-Visna virus were selected. Several clones of p25 specific scFv antibodies were identified; one of th...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931380
更新日期:2003-01-01 00:00:00
abstract::Fourteen of 200 Lactobacillus isolates from African fermented foods, viz. 'wara', 'kenkey', 'ugba', 'ogi', 'kunuzarki', 'fufu' and 'iru' were found to produce bacteriocins against L. plantarum and only three bacteriocinogenic isolates inhibited some of the food pathogens. Plasmid analysis of the 14 bacteriocin-produci...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02814645
更新日期:1994-01-01 00:00:00
abstract::Bacterial metabolites with communicative functions could provide protection against stress conditions to members of the same species. Yet, information remains limited about protection provided by metabolites in Bacillus cereus and inter-species. This study investigated the effect of extracellular compounds derived fro...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-016-0487-2
更新日期:2017-05-01 00:00:00
abstract::The regulation of gene transcription allows yeast cells to respond properly to changing environmental conditions. Several protein complexes take part in this process. They involve RNA polymerase complexes, chromatin remodeling complexes, mediators, general transcription factors and specific transcriptional regulators....
journal_title:Folia microbiologica
pub_type: 杂志文章,评审
doi:10.1007/s12223-008-0044-8
更新日期:2008-01-01 00:00:00
abstract::The synthesis of exocellular proteinase decreases with increasing concentration of amino acids in the growth medium. After removal of amino acids the enzyme synthesis is gradually restored to normal values. The presence of inhibitors of transcription (actinomycin D) or translation (chloramphenicol) blocks the restorat...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02875957
更新日期:1984-01-01 00:00:00
abstract::Due to the nature of Vibrio vulnificus infections (i.e., gastroenteritis and septicemia), only very few studies of a biofilm-associated form in this pathogen's life cycle have been conducted. We proposed that biofilm production by clinical strains of V. vulnificus would be higher than by environmental strains. Biofilm...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-019-00761-9
更新日期:2020-06-01 00:00:00
abstract::Using an in vitro cytotoxicity assay (51Cr-release), it was shown that bilirubin exerts a cytotoxic effect on both adult and newborn human lymphocytes after a 1-d incubation of cells with bilirubin. The effect of bilirubin on human granulocytes was less pronounced; the association of the cytotoxic effect with a functi...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02923520
更新日期:1985-01-01 00:00:00
abstract::There is a growing interest in the use of bioinoculants to assist mineral fertilizers in improving crop production and yield. Azotobacter and Pseudomonas are two agriculturally relevant strains of bacteria which have been established as efficient bioinoculants. An experiment involving addition of graded concentrations...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-017-0495-x
更新日期:2017-05-01 00:00:00
abstract::Strains of Rhodospordium torloides produced two peptides into the culture filtrate. Peptide I contained 6 amino acids and peptide II contained 5 amino acids. The peptides however, are, not homologous to the alpha-factor, and become assimilated at a later phase of growth. ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02876614
更新日期:1980-01-01 00:00:00
abstract::We isolated and identified functional groups of bacteria in the rumen of Creole goats involved in ruminal fermentation of native forage shrubs. The functional bacterial groups were evaluated by comparing the total viable, total anaerobic, cellulolytic, hemicellulolytic, and amylolytic bacterial counts in the samples t...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-012-0219-1
更新日期:2013-09-01 00:00:00
abstract::Ovine and caprine scrapie occupies a unique place among animal transmissive spongiform encephalopathies (TSE). It is an object of intensive biomedicinal, ecological and economical studies. Its causative agents are demonstrably associated with the development of TSE in farmed minks, goats and moufflons. Ovine strains o...
journal_title:Folia microbiologica
pub_type: 杂志文章,评审
doi:10.1007/BF02818713
更新日期:2000-01-01 00:00:00
abstract::The bioluminescence (BLM) and colony-forming units (CFU) of Pseudomonas fluorescens HK44 were monitored during encapsulation into pre-polymerized Si(OMe)₄. The non-induced BLM of free cells was increased in the presence of 0.5-2.5 % MeOH. After mixing silica sol with the cell suspension, both BLM and CFU dropped to 1-...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-010-0091-9
更新日期:2010-11-01 00:00:00
abstract::Plenty of metagenomic studies have suggested possible associations between microbiome composition and colorectal cancer (CRC). However, these techniques are not economic enough for routine use so far. Therefore, we explored the possibility to detect species associated with colorectal cancer by conventional culture fro...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-020-00807-3
更新日期:2020-12-01 00:00:00
abstract::Antibacterial effect of chitooligosaccharides (COS) and low molar mass chitosans (LMWC) is considered as one of the most important characteristics of chitosan (CS) hydrolysates. Here, we show the in vitro effect of different COS, LMWC, and CS on representative anaerobic bacteria isolated from human colon as a possibil...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-012-0138-1
更新日期:2012-07-01 00:00:00
abstract::Using simultaneous fusion of protoplasts of three strains the auxotrophic K3 killer designated MH1 was prepared at a very low frequency and further employed for the transfer of the K3 killer character into a commercial wine yeast. A novel Saccharomyces Bratislava 1K3 strain with desirable technological and anti-yeast ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02814566
更新日期:1992-01-01 00:00:00
abstract::A quarantine organism, "Candidatus Phytoplasma mali," is the causal agent of apple proliferation, one of the most important apple diseases in Europe. The genetic diversity of this pathogen in Central and Southern Europe has already been reported; however, almost no data exists from Eastern Europe. In this study, "Ca. ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-018-0660-x
更新日期:2019-05-01 00:00:00
abstract::The mean lengths of G1, S, G2 and M phases of the cell cycle were determined on the basis of the population distribution of Candida utilis grown in a continuous culture under steady-state conditions by using an original mathematical method. The length of the G2 phase was proportional to that of G1; the length of M was...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02821281
更新日期:1990-01-01 00:00:00
abstract::The treatment of three patients suffering from chronic bacterial prostatitis who were qualified for an experimental phage therapy protocol managed at the Phage Therapy Unit in Wrocław is described. They had previously been treated unsuccessfully with long-term targeted antibiotics, autovaccines, and laser biostimulati...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-009-0064-z
更新日期:2009-09-01 00:00:00
abstract::A rifampicin-resistant variant of two strains of Lactobacillus plantarum, one strain of Pediococcus acidilactici, and one strain of Enterococcus faecium were used for the experimental production of lucerne silage. Laboratory silage without inoculants served as a control. Counts of total anaerobes, total lactic acid ba...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-012-0142-5
更新日期:2012-07-01 00:00:00