Abstract：
:Analysis of actA gene sequence polymorphism has been shown to be an effective and relatively inexpensive method for subtyping Listeria monocytogenes isolates, allowing the division of the population of this species into two deeply separate lineages. This sequence-based method as well as PCR-mediated fingerprinting were applied here for the differentiation of 49 isolates of food and clinical origin. Correlation between these two typing approaches was high. Both methods divided the isolates into two lineages, designated I (33 isolates) and II (16 isolates). All the 33 lineage I isolates were assigned to the same, or closely related, six clusters by both typing methods. For the lineage II isolates, PCR fingerprinting was found to be more discriminatory. The isolates were characterized by cell invasion assay. All highly invasive isolates were assigned to lineage I, which constituted a heterogeneous group also containing low-invasive isolates. High-invasive isolates were not found in the genetically determined lineage II. A particular actA cluster, designated Ha, contained all the isolates showing the lowest invasiveness. A common trait of the isolates belonging to this cluster was the presence of a threonine-441 of the deduced ActA sequence instead of the alanine-441 present in the remaining isolates. Thirteen human isolates were classified to lineage I and five to lineage II. A PCR-based method can therefore differentiate L. monocytogenes isolates in accordance with the current phylogenetic model of the evolution of this species.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Bania J,Zarczyńska A,Molenda J,Dabrowska A,Kosek-Paszkowska K,Wieckowska-Szakiel M,Rózalska Bdoi
10.1007/s12223-009-0003-zsubject
Has Abstractpub_date
2009-01-01 00:00:00pages
17-24issue
1eissn
0015-5632issn
1874-9356journal_volume
54pub_type
杂志文章abstract：:Single chain Fv (scFv) antibodies (generated by phage display technology, molecules representing new and efficient tools in the research and diagnostics of infectious diseases) against the capsid protein (p25) of Maedi-Visna virus were selected. Several clones of p25 specific scFv antibodies were identified; one of th...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931380
更新日期：2003-01-01 00:00:00
abstract：:Viridans group streptococci bloodstream infections (VGS BSI) remain a significant cause of mortality and morbidity in patients with severe neutropenia. The goal of our study was to evaluate clinical course and microbiological susceptibility of VGS BSI at our center. Retrospective analysis of all microbiologically docu...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-017-0542-7
更新日期：2018-03-01 00:00:00
abstract：:Using fluorescein-labelled antibodies against gamma, mu and alpha chains, Ig-containing cells in palatine tonsils were studied in 120 patients. The aim of this study was to determine the most frequently repeated typical findings as regards the numbers and localisation of these cells in tonsils and to confront the data...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02927432
更新日期：1981-01-01 00:00:00
abstract：:The production of bacterial enzymes in batch fermentations is compared with results obtained in continuous culture. When studying the production of alpha-amylase in Bacillus subtilis it was found that instability of the enzyme synthesis was due to nonhomogeneity of the population rather than to "the culture's history"...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02883137
更新日期：1982-01-01 00:00:00
abstract：:Fifty Escherichia coli strains isolated from stool samples of 51 healthy children, 143 strains isolated from stool samples of 327 children with diarrhea and 24 strains isolated from stool samples of 21 children with suspected hemolytic uremic syndrome were examined for the presence of Shiga toxin-producing E. coli vir...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02817680
更新日期：2002-01-01 00:00:00
abstract：:The aim of the study was to compare the phenotype of lymphocyte subpopulations of the GALT (gut-associated lymphatic tissue) in germfree (GF) and conventionally (CV) reared rats, i.e. to analyze the effect of microbial colonization on the development of intestinal lymphocyte subsets. Surface marker characteristics wer...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02820814
更新日期：1998-01-01 00:00:00
abstract：:Several amino acids affected the amount of bacitracin produced by Bacillus licheniformis ATCC 10716 and the high-yielding mutant B. licheniformis AL. The possibility of an amino acid control mechanism for bacitracin biosynthesis is discussed. The two strains showed a different response to the addition of some amino ac...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02926454
更新日期：1979-01-01 00:00:00
abstract：:The mean lengths of G1, S, G2 and M phases of the cell cycle were determined on the basis of the population distribution of Candida utilis grown in a continuous culture under steady-state conditions by using an original mathematical method. The length of the G2 phase was proportional to that of G1; the length of M was...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02821281
更新日期：1990-01-01 00:00:00
abstract：:Bacteria accumulate high amounts of potassium in the cytoplasm. For studying transport of K+ (with 86Rb as a marker) in bacteria (Staphylococcus aureus 17810S), the cells were depleted of the internal K+ pool by a DNP treatment. Kinetics and energetics of 86Rb transport was assayed with glucose as an exogenous energy ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02816378
更新日期：1998-01-01 00:00:00
abstract：:The present study was conducted to determine the gene responsible for beta-glucosidase (BGL) production and to generate a full-length complementary DNA (cDNA) of one of the putative BGL genes, which showed a significant expression level when Schizophyllum commune KUC9397 was grown in optimized medium. The relative exp...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-016-0484-5
更新日期：2017-05-01 00:00:00
abstract：:Crude lipids isolated from Bacillus firmus, but not from other bacilli, were previously found to induce significant resistance against Listeria monocytogenes infection in mice. In this study, formaldehyde- and heat-killed bacterins of eight Bacillus species and some cellular fractions of B. firmus were prepared and te...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02906811
更新日期：1994-01-01 00:00:00
abstract：:Mycobacterium microti-infected mouse peritoneal macrophages produced high amounts of prostaglandin E2 (PGE2) and nitric oxide (NO) when activated with interferon-gamma (IFN-gamma). In order to understand the relation between PGE2 and NO production and the expression of interleukin-12 (IL-12), interleukin-10 (IL-10) an...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02818541
更新日期：2001-01-01 00:00:00
abstract：:Among 18 tested strains of Claviceps spp., 7 produced significant amounts of exocellular polysaccharide (EPS). The maximum production of EPS was found in fermentation broth of Claviceps viridis. The kinetics of growth, substrate consumption, and EPS production in the batch, aerobic, submerged culture of this fungus we...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02993470
更新日期：2003-01-01 00:00:00
abstract：:Phytoplankton successions under ice cover (January-March) were determined in four oligotrophic lakes (Burtjärn, Aspen, Vialamptjärn and Storkorstjärn) located in North-Eastern Sweden. The total phosphorus concentration in the lakes was less than 10 micrograms/L. Lake Burtjärn (reference lake) had a similar hydrology a...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931370
更新日期：2003-01-01 00:00:00
abstract：:Staphylococcus epidermidis is a biofilm-forming bacterial strain that can cause major problems as an agent of nosocomial infections. Bacteria in biofilms are shielded from the environment and can survive high doses of antibiotics. We here test the antibiotic susceptibility of Staphylococcus epidermidis to rising genta...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-017-0568-x
更新日期：2018-05-01 00:00:00
abstract：:The soil microbial population, determined over a period of 19 months and the elemental chemical composition measured by X-ray fluorescence techniques were followed in two uncultivated salt-affected areas of Greece, namely Lantza and Klidi. The ecological conditions at Lantza remained stable during the period of study ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02877387
更新日期：1983-01-01 00:00:00
abstract：:Cyanobacteria are one of the most successful and oldest forms of life that are present on Earth. They are prokaryotic photoautotrophic microorganisms that colonize so diverse environments as soil, seawater, and freshwater, but also stones, plants, or extreme habitats such as snow and ice as well as hot springs. This d...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/s12223-018-0626-z
更新日期：2018-09-01 00:00:00
abstract：:Using mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine, a number of homoserine auxotrophs have been isolated from a glutamate-producing Arthrobacter globiformis excreting L-lysine in good amounts. For further improvement, mutants resistant to the lysine analog S-(2-aminoethyl)-L-cysteine have been isolated from h...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02935823
更新日期：1991-01-01 00:00:00
abstract：:A method is presented for the successful decontamination of sparingly soluble pteridine derivatives by microwave irradiation. The method is nondestructive, rapid and effective in eliminating contamination. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814514
更新日期：1991-01-01 00:00:00
abstract：:Numerous antibodies with a known mechanism of action are utilized as possible means for studying morphogenesis and differentiation. Inhibitors of biosynthesis of nucleic acids and proteins, compounds intervening with the synthesis and/or function of cell walls and membranes or compounds influencing the energy metaboli...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/BF02897219
更新日期：1980-01-01 00:00:00
abstract：:The effects of pH on denitrifying enzyme activity (DEA) and on the ratio of the denitrification products, N(2)O and N(2), were determined in three pasture soils differing in cattle impact. The linkage between intrinsic differences in the denitrifying communities and pH effects on relative N(2)O production was also ass...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-011-0045-x
更新日期：2011-05-01 00:00:00
abstract：:Fungal cells of Aureobasidium pullulans ATCC 201253 were immobilized by entrapment in chitosan beads, and the immobilized cells were investigated for their ability to produce the polysaccharide pullulan using batch fermentation. The 1% chitosan-entrapped fungal cells were capable of producing pullulan for two cycles o...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-011-0048-7
更新日期：2011-07-01 00:00:00
abstract：:The rosettes formed by mouse peritoneal macrophages or DCH-5 cells and TNP-erythrocytes coated with anti-TNP antibodies of different isotypes were inhibited to various extent by monosaccharides. The most effective inhibitors were N-acetylglucosamine, glucosamine, mannose and N-acetylneuraminic acid in 1-5 mmol/L conce...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814584
更新日期：1992-01-01 00:00:00
abstract：:The green alga Scenedesmus intermedius was grown in synchronous culture under manganese or calcium deficiency for six successive generations. The growth rate, pigment and protein contents gradually decreased in comparison with the control. In Mn-deficient cells, the rate of oxygen evolution was sharply decreased. This...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02817561
更新日期：2000-01-01 00:00:00
abstract：:The addition of a nitrogen (nitrate) and carbon sources (acetate, citrate and fructose) and phosphate deficiency (nitrate medium deficient in phosphate) under unaerated conditions induced akinete differentiation in Anabaena torulosa. Aerated cultures of this organism in these nutrients did not differentiate akinetes. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02817617
更新日期：2000-01-01 00:00:00
abstract：:A 6.5 kb DNA fragment containing the gene (thrC) encoding threonine synthase, the last enzyme of the threonine biosynthetic pathway, has been cloned from the DNA of Bacillus sp. ULM1 by complementation of Escherichia coli and Brevibacterium lactofermentum thrC auxotrophs. Complementation studies showed that the thrB g...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02818515
更新日期：1995-01-01 00:00:00
abstract：:Streptomyces caelestis DSM 40084 produces two osmolytes, viz. 2-O-(alpha-D-glucopyranosyl)-zeta-glyceric acid (GG) and trehalose. Both compounds were isolated and identified by nuclear magnetic resonance spectroscopy and mass spectrometry. A very sensitive regulation of the cell osmolytes was demonstrated in exponenti...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02932103
更新日期：2007-01-01 00:00:00
abstract：:An economic method for a rapid estimation of the number of copies of plasmid R6K delta 1 in E. coli cells using lysis of the cells directly on the agarose gel and electrophoretic separation of radioactively labelled plasmid and chromosomal DNA's is described. The method, particularly useful for screening purposes, per...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02879773
更新日期：1982-01-01 00:00:00
abstract：:The potentiality of twenty-five fungal species belonging to 14 genera isolated from Egyptian soils to produce mevinolin, a hypocholesterolemic agent, when grown on selected substrates was tested. For the first screening samples of culture filtrates were tested by TLC and the positive results were further estimated by ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02826557
更新日期：1997-01-01 00:00:00
abstract：:Procedures to introduce point mutations, restriction sites and insert or delete DNA fragments are very important tools to study protein function. We describe here two-step PCR-based method for generating single or multiple mutations, insertions and deletions in a small region of the sequence. In the first step, a uniq...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02816213
更新日期：1999-01-01 00:00:00
