Abstract:
:Crude lipids isolated from Bacillus firmus, but not from other bacilli, were previously found to induce significant resistance against Listeria monocytogenes infection in mice. In this study, formaldehyde- and heat-killed bacterins of eight Bacillus species and some cellular fractions of B. firmus were prepared and tested for further immunomodulatory activities. Crude lipids, their aqueous extract, LTA, Protodyne and Pex-residue preparations exhibited a strong anti-infection activity, whereas Pextract, P40 and all bacterins tested had no effect. Formaldehyde-killed bacterins, live bacteria and the P40 preparation of both B. firmus strains, as well as bacterins of both B. subtilis strains, induced pronounced splenomegaly in mice. Peptidoglycan and Pex-residue induced significant depression of cytochrome P-450 in mouse liver microsomes after application of 0.1 mg per mouse. Optimal conditions for obtaining a bacterial suspension exhibiting these immunomodulatory properties were elaborated.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Mára M,Ocenásková J,Nováková M,Julák J,Mencíková Edoi
10.1007/BF02906811subject
Has Abstractpub_date
1994-01-01 00:00:00pages
147-51issue
2eissn
0015-5632issn
1874-9356journal_volume
39pub_type
杂志文章abstract::A method is presented for the successful decontamination of sparingly soluble pteridine derivatives by microwave irradiation. The method is nondestructive, rapid and effective in eliminating contamination. ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02814514
更新日期:1991-01-01 00:00:00
abstract::One-hundred patients with candidosis of the skin, mucous membranes and nail plates (confirmed by cultivation) were examined. Topical or systemic antimycotic treatment was successful in 58 patients. After a complete evaluation, 42 patients were found to suffer from factors supporting candidosis--diabetes mellitus (12),...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02815627
更新日期:2001-01-01 00:00:00
abstract::Glutamate was converted to glutamine by Corynebacterium glutamicum permeabilized by ethanol (10%). ATP was supplied to the reaction by dried Saccharomyces cerevisiae or regenerated by acetyl kinase. High glutamine synthetase activity in C. glutamicum was induced by cultivation of the microorganism in media containing ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02816248
更新日期:1999-01-01 00:00:00
abstract::Cardiolipin synthesis was studied during the aerobic synchronous growth of aerobically grown yeast Saccharomyces cerevisiae. The time course of the synthesis was stepwise and the rise in cardiolipin level in cells coincided in time with the increase in cytochrome oxidase activity. This finding supports the notion of d...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02876990
更新日期:1977-01-01 00:00:00
abstract::Manganese peroxidase (MnP) was produced by shallow stationary cultures of Phanerochaete chrysosporium growing on N-limited medium. Decolorization of sulfonphthalein (SP) dyes by MnP was investigated. The MnP activity profile and decolorization of SP dyes was correlated and almost all dyes were decolorized at pH 4.0. T...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931512
更新日期:2003-01-01 00:00:00
abstract::In the present study, the cDNA sequences of Hsp70 and Hsp90 genes of Isaria farinosa (designated IFHSP70 and IFHSP90) were cloned and characterized using multiple techniques of molecular biology and bioinformatics. The genetic differentiation of the two genes was investigated among 10 geographically separated populati...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-018-00676-x
更新日期:2019-07-01 00:00:00
abstract::One hundred clinical isolates of Pseudomonas aeruginosa were checked for their sensitivity towards silver nitrate. Majority of the isolates were resistant at 20 mg/L and the resistance decreased with increasing concentration of silver nitrate, only 5% of the organisms showed resistance above 70 mg/L. These silver-resi...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02820752
更新日期:1989-01-01 00:00:00
abstract::Uropathogenic Escherichia coli (UPEC) isolates contain large genomic segments, termed pathogenicity islands (PAIs), that contribute to their virulence. A total of 150 UPEC and 50 commensal E. coli isolates from outpatients were investigated for antimicrobial susceptibility and the presence of eight PAI markers. One hu...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-015-0433-8
更新日期:2016-05-01 00:00:00
abstract::A selection of tert-butylhydroperoxide (tBOOH)-tolerant Candida albicans mutants showed increased tolerances to 19 different stress conditions. These mutants are characterized by a constitutively upregulated antioxidative defense system and, therefore, adaptation to oxidative stress may play an important role in gaini...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-014-0305-7
更新日期:2014-07-01 00:00:00
abstract::Extrusion of metabolites (glycerol, lactic, malic, and succinic acid) during the medium acidification caused by resting baker's yeast supplied with 200 mM glucose was studied under aerobic and anaerobic conditions and in the absence and presence of 14 mM KCl. The maximum levels of glycerol and of the sum of acids (abo...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02876611
更新日期:1980-01-01 00:00:00
abstract::Data on the production of clostridial toxins in dialyzed cultures are summarized. Principal modifications of this cultivation technique suitable for both research and production are shown. If toxins are released from the cells by autolysis (neurotoxins of Clostridium tetani, C. botulinum; lethal factors of C. novyi an...
journal_title:Folia microbiologica
pub_type: 杂志文章,评审
doi:10.1007/BF02928083
更新日期:1988-01-01 00:00:00
abstract::Dithiocarbamates were found to enhance the production of citric acid under solid-state fermentation conditions by Aspergillus niger. Maximum increase was observed with tetramethylthiuram disulfide (TMTD). Percent increases observed were 74.2% with 2.5 micrograms/mL of TMTD, 19.6% with 2.5 micrograms/mL of sodium dimet...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02926953
更新日期:1986-01-01 00:00:00
abstract::The emergence of spa types and spa-clonal complexes (CC) among clinical methicillin-resistant Staphylococcus aureus isolates collected from the University Clinical Center in Gdańsk between 2008 and 2009 were investigated. Phage typing was used as the initial screening in the study. The basic set of phages and the addi...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-012-0148-z
更新日期:2012-05-01 00:00:00
abstract::A total of 40 substances were tested for their inhibitory effect on the multiplication of a bacteriophage in a growing culture of Bacillus licheniformis and their influence on bacitracin production. Acriflavine was the only substance which, at a concentration of 3 microgram ml-1, completely suppressed phage multiplica...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02876597
更新日期:1978-01-01 00:00:00
abstract::This work was aimed to develop a multiplex PCR assay to detect infectious agents such as Clavibacter michiganensis subsp. michiganensis, Fusarium sp, Leveillula taurica, and begomoviruses in tomato (Solanum lycopersicum) plants. Specific primer sets of each pathogen were designed based on intergenic ribosomal RNA sequ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-012-0206-6
更新日期:2013-07-01 00:00:00
abstract::Ultrastructural changes induced by Rickettsia slovaca standard type (ST) and wild type (WT) were examined during their life cycle in L929 and Vero cells. R. slovaca invaded the cytoplasm of the host cell by phagocytosis on the 1st d p.i. Rickettsiae adhering to the cytoplasmic membrane were engulfed by cellular extens...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-009-0019-4
更新日期:2009-01-01 00:00:00
abstract::A strain of Aspergillus giganteus cultivated in a medium with xylan produced two xylanases (xylanase I and II) which were purified to homogeneity. Their molar mass, estimated by SDS-PAGE, were 21 and 24 kDa, respectively. Both enzymes are glycoproteins with 50 degrees C temperature optimum; optimum pH was 6.0-6.5 for ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931639
更新日期:2004-01-01 00:00:00
abstract::Inducible pigmentation changes were observed in pigmented strains of Brevibacterium sp. M27 and B. flavum treated with N-methyl-N'-nitro-N-nitrosoguanidine. The highest frequency of induction was reached already at a survival of 30-40% with the maximal yield of 6-10%. As compared with the initial yellow colour, three ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02928007
更新日期:1988-01-01 00:00:00
abstract::Two classes of newly synthesized amphiphilic compounds, phenolic antioxidants ("phenolics") and N-oxides exert in vivo antioxidant effects on live S. cerevisiae cells. Both groups have low toxicity, phenolics being more toxic than N-oxides and compounds with a longer alkyl chain having higher toxicity than those with ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02932187
更新日期:2007-01-01 00:00:00
abstract::Inhibition of methanogenesis in ruminal cultures was attempted by hindering thiamine availability through its degradation by 'polyphenols' and competition for active sites on enzymes and transporters using thiamine structural analogs. Effects on fermentation were small and not consistently reversed by adding thiamine....
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-009-0075-9
更新日期:2009-11-01 00:00:00
abstract::Over the years, quinones or its derivatives have been extensively studied due to their broad therapeutic spectrum. However, due to the significant structural differences between the individual naturally occurring quinones, investigation of the precise mechanism of their action is essential. In this context, we have an...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-019-00722-2
更新日期:2020-02-01 00:00:00
abstract::It is proposed that the transcription of the c-2 repressor gene initiates at two promoters, pre and prm, similarly to the transcription of the c-I gene of lambda phage. In the P22 phage, these promoters are defined by mutations of c54 and K5. ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02876780
更新日期:1975-01-01 00:00:00
abstract::In the present study, 193 Aspergillus strains were isolated from a total of 100 soil samples of pistachio orchards, which all of them were identified as Aspergillus flavus as the most abundant species of Aspergillus section Flavi existing in the environment. Approximately 59%, 81%, and 61% of the isolates were capable...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-011-0090-5
更新日期:2012-01-01 00:00:00
abstract::Frequency of heterocytes and nitrogenase activity (NA) under light and dark cultivation conditions was determined in 12 cyanobacterial strains isolated from various soil habitats. In spite of a high variability, significant differences in NA among the strains were found in response of light and dark cultivation. Relat...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931606
更新日期:2004-01-01 00:00:00
abstract::Single chain Fv (scFv) antibodies (generated by phage display technology, molecules representing new and efficient tools in the research and diagnostics of infectious diseases) against the capsid protein (p25) of Maedi-Visna virus were selected. Several clones of p25 specific scFv antibodies were identified; one of th...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931380
更新日期:2003-01-01 00:00:00
abstract::Changes in leukocyte counts and in the gut microflora of laboratory rats irradiated with single whole-body dose of gamma rays (5.0 Gy) were determined. The number of leukocytes was lower especially 1 and 2 weeks after irradiation. A significant decrease in lymphocytes was observed 1 week and in monocytes 1 and 2 weeks...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02818710
更新日期:2002-01-01 00:00:00
abstract::Cryptosporidiosis belongs to the important parasitic infections with zoonotic potential and the occurrence in European countries is rare. The first cases of cryptosporidiosis caused by Cryptosporidium hominis detected in the Slovak republic were described here. Collection of examined humans consisted of five family me...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-012-0182-x
更新日期:2013-01-01 00:00:00
abstract::The root nodules of Phaseolus mungo (a herbaceous leguminous pulse) contained a high amount of 3-indolylacetic acid (IAA). A tryptophan pool present in the nodule might play the role of precursor for IAA production. From the root nodule a Rhizobium sp. was isolated. The symbiont produced a large amount of IAA (142 mic...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-008-0054-6
更新日期:2008-01-01 00:00:00
abstract::Candida bracarensis is an emerging cryptic species within the Candida glabrata clade. To date, little is known about its epidemiology, virulence, and antifungal susceptibility. This study documents the occurrence of C. bracarensis for the first time in Mexico and focuses on its in vitro production of hydrolytic enzyme...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-018-0592-5
更新日期:2018-07-01 00:00:00
abstract::beta-Glucosidase from the fungus Thermoascus aurantiacus grown on semi-solid fermentation medium (using ground corncob as substrate) was partially purified in 5 steps--ultrafiltration, ethanol precipitation, gel filtration and 2 anion exchange chromatography runs, and characterized. After the first anion exchange chro...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02818672
更新日期:2002-01-01 00:00:00