Abstract：
:An economic method for a rapid estimation of the number of copies of plasmid R6K delta 1 in E. coli cells using lysis of the cells directly on the agarose gel and electrophoretic separation of radioactively labelled plasmid and chromosomal DNA's is described. The method, particularly useful for screening purposes, permits detection of both the CCC and OC forms of plasmids of molar mass 2-150 Mg/mol and it can be applied to other bacterial systems.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Hochmannová J,Nesvera Jdoi
10.1007/BF02879773subject
Has Abstractpub_date
1982-01-01 00:00:00pages
138-41issue
2eissn
0015-5632issn
1874-9356journal_volume
27pub_type
杂志文章abstract：:Vinasse, a recalcitrant waste of the ethanol industry was employed for the production of polyhydroxyalkanoate (PHA) by the extremely halophilic archaeon, Haloarcula marismortui in shake flasks. The PHA was recovered by osmotic lysis of the cells and subsequent purification by sodium hypochlorite and organic solvents. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-011-0092-3
更新日期：2012-01-01 00:00:00
abstract：:In the present study, 193 Aspergillus strains were isolated from a total of 100 soil samples of pistachio orchards, which all of them were identified as Aspergillus flavus as the most abundant species of Aspergillus section Flavi existing in the environment. Approximately 59%, 81%, and 61% of the isolates were capable...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-011-0090-5
更新日期：2012-01-01 00:00:00
abstract：:Aeromonads represent bacteria thought to be primarily mostly autochthonous to aquatic environments. This study was focused on the relation with antibiotics and enterocins of identified Aeromonas species isolated from the intestine of trouts living in Slovakian aquatic sources. Intestinal samples from 50 trouts (3 Salm...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-018-0616-1
更新日期：2018-11-01 00:00:00
abstract：:The chloramphenicol resistance gene from pSa was introduced into T-DNA of pTi T37 of Agrobacterium tumefaciens by cointegration with intermediary plasmid based on pBR322. The resulting intermediary vector was mobilized to A. tumefaciens T37 by conjugative plasmid pRK2. The RK2 plasmid also forms contegrates with pTi d...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02926824
更新日期：1986-01-01 00:00:00
abstract：:The addition of a nitrogen (nitrate) and carbon sources (acetate, citrate and fructose) and phosphate deficiency (nitrate medium deficient in phosphate) under unaerated conditions induced akinete differentiation in Anabaena torulosa. Aerated cultures of this organism in these nutrients did not differentiate akinetes. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02817617
更新日期：2000-01-01 00:00:00
abstract：:Biotechnological conversion of low-cost agro-industrial by-products, such as industrial waste or terpenes from the distillation of essential oils from plants into more valuable oxygenated derivatives, can be achieved by using microbial cells or enzymes. In Argentina, the essential oil industry produces several tons of...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-015-0422-y
更新日期：2016-03-01 00:00:00
abstract：:Genetic mapping of the chromosome of the bacteriophage PS8 of Agrobacterium tumefaciens using temperature sensitive (ts) mutants of the bacteriophage is described. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02927304
更新日期：1979-01-01 00:00:00
abstract：:The gene encoding the coat protein (CP) of a potato virus Y (PVY) was cloned into expression vector pMPM-A4Omega. PVY CP was expressed in Escherichia coli and the purified recombinant protein was used for raising rabbit polyclonal antibodies. The sera and antibodies were tested for the detection of PVY in the laborato...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-008-0067-1
更新日期：2008-01-01 00:00:00
abstract：:Oxygen uptake by the spores of Fusarium moniliforme, F. oxysporum, F. semitectum, F. solani, Mucor racemosus and Trichoderma viride was increased in the presence of volatile substances extracted from Origanum majorana and Ocimum basilicum. This increase was greater in the presence of volatile substances from O. basili...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02885580
更新日期：1978-01-01 00:00:00
abstract：:As phospholipases of mycoplasma species may play a role in the pathogenesis of respiratory tract and urogenital tract diseases Mycoplasma mycoides and Acholeplasma laidlawii were examined as to the production of phospholipase A2 (PLA) and attempts were made to purify and characterize it. Both species produced PLA. The...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814471
更新日期：1989-01-01 00:00:00
abstract：:Lactobacillus pobuzihii is a novel species which has been previously found in pobuzihi (fermented cummingcordia), a traditional fermented food in Taiwan. However, the lactic acid bacteria (LAB) microflora in pobuzihi has not been studied in detail. In this study, LAB from pobuzihi were isolated, identified, and charac...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-012-0188-4
更新日期：2013-03-01 00:00:00
abstract：:Strains of fungi imperfecti of genera Aspergillus and Rhizopus were tested for the ability to produce alpha-mannosidases. The most suitable alpha-mannosidase producer of a total of 20 strains under study was Aspergillus Ravus Link 69. The parameters studied during the cultivation included the growth rate expressed as ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02876433
更新日期：1978-01-01 00:00:00
abstract：:In yeast, the STB5 gene encodes a transcriptional factor belonging to binuclear cluster class (Zn2Cys6) of transcriptional regulators specific to ascomycetes. In this study, we prepared the Kluyveromyces lactis stb5Δ strain and assessed its responses to different stresses. We showed that KlSTB5 gene is able to complem...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-019-00682-7
更新日期：2019-07-01 00:00:00
abstract：:The bioluminescence (BLM) and colony-forming units (CFU) of Pseudomonas fluorescens HK44 were monitored during encapsulation into pre-polymerized Si(OMe)₄. The non-induced BLM of free cells was increased in the presence of 0.5-2.5 % MeOH. After mixing silica sol with the cell suspension, both BLM and CFU dropped to 1-...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-010-0091-9
更新日期：2010-11-01 00:00:00
abstract：:The present study was designed to characterize six Trueperella (T.) abortisuis strains, cultured over a period of 5 months from fetus and abortion material of six pigs of a single farm in Mecklenburg-West Pomerania federal state, Germany. It was of interest to investigate the epidemiological relationships of the six s...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-019-00753-9
更新日期：2020-06-01 00:00:00
abstract：:Based on the ability to attach to polymeric surfaces, the formation of biofilms was determined in 5 wild-type strains (Pseudomonas aeruginosa, Klebsiella pneumoniae, Acinetobacter baumanii, Escherichia coli, Staphylococcus warneri). Using modified Christensen method, minimum regrowth concentration (MRC) of piperacilli...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931650
更新日期：2004-01-01 00:00:00
abstract：:Uropathogenic Escherichia coli (UPEC) isolates contain large genomic segments, termed pathogenicity islands (PAIs), that contribute to their virulence. A total of 150 UPEC and 50 commensal E. coli isolates from outpatients were investigated for antimicrobial susceptibility and the presence of eight PAI markers. One hu...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-015-0433-8
更新日期：2016-05-01 00:00:00
abstract：:Using fermentation with a selected strain of Acetomonas oxydans it was possible to convert 2-deoxy-2-fluro-D-glucitol to 5-deoxy-5-fluoro-L-sorbose, in agreement with Bertrand's and Hudson's rule. The last-named compound was isolated in a yield of 88%. Both compounds were little toxic against Acetomonas oxydans. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02877659
更新日期：1977-01-01 00:00:00
abstract：:Hemolytic factor production by pathogenic Candida species is considered an important attribute in promoting survival within the mammal host through the ability to assimilate iron from the hemoglobin-heme group. Hemolytic capability has been evaluated for Candida species based on hemolysis zones on plate assay, analysi...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/s12223-018-0584-5
更新日期：2018-07-01 00:00:00
abstract：:Five trisubstituted quinazolones and eight trisubstituted quinazoline-4-thiones have been tested for antibacterial effects by a microdilution method. Four derivatives exerted a significant effect on E. coli, P. aeruginosa, S. aureus and B. subtilis (IC50 < 100 mg/L). In the bacterium P. aeruginosa six quinazolines sho...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02817411
更新日期：2000-01-01 00:00:00
abstract：:A hexokinase preparation was obtained from a Saccharomyces cerevisiae mutant strain deficient in glucosephosphate isomerase (GPI) and mannosephosphate isomerase (MPI) by precipitation with ammonium sulfate. The supernatant fraction corresponding to 40-60 % saturation showed the lowest content in GPI and MPI activity. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02927364
更新日期：1981-01-01 00:00:00
abstract：:Aminoglycosides at 2x or 4x minimum inhibitory concentration induced postantibiotic effects against Pseudomonas aeruginosa lasting 3.5-4.9 h (gentamicin) and 0.5-3.7 h (selemycin). Postantibiotic effects of subinhibitory concentrations of the aminoglycosides tested were substantially longer. Some combinations of supra...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814691
更新日期：1996-01-01 00:00:00
abstract：:The biosynthetic gene cluster of porothramycin, a sequence-selective DNA alkylating compound, was identified in the genome of producing strain Streptomyces albus subsp. albus (ATCC 39897) and sequentially characterized. A 39.7 kb long DNA region contains 27 putative genes, 18 of them revealing high similarity with hom...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-014-0339-x
更新日期：2014-11-01 00:00:00
abstract：:Different mechanistic models for potassium ion uptake are analyzed by an equilibrium-thermodynamic formalism in terms of their comparative efficiency in setting chemical potential differences of the potassium ion of different magnitudes across the plasma membrane of lower eukaryotic cells. The possible adaptive advant...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/BF02925852
更新日期：1988-01-01 00:00:00
abstract：:Competitive PCR method was developed for the detection and enumeration of Butyrivibrio fibrisolvens. Sequences of 16S rDNA were obtained from our isolates (serving as a source of data for primer design) and were distinguished into nine different groups of butyrivibria. Specific primers for two distinct groups were des...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02825888
更新日期：2001-01-01 00:00:00
abstract：:Using phenol--water extraction, a lipopeptidopolysaccharide complex (LPPS) was isolated from Listeria monocytogenes. Some biological and immunological properties of LPPS were compared with lipopolysaccharide isolated by the same procedure from Salmonella typhimurium. LPPS possesses low pyrogenicity, but the immunologi...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02923515
更新日期：1985-01-01 00:00:00
abstract：:Numerous antibodies with a known mechanism of action are utilized as possible means for studying morphogenesis and differentiation. Inhibitors of biosynthesis of nucleic acids and proteins, compounds intervening with the synthesis and/or function of cell walls and membranes or compounds influencing the energy metaboli...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/BF02897219
更新日期：1980-01-01 00:00:00
abstract：:Candidiases, infections caused by germination forms of the Candida fungus, represent a heterogeneous group of diseases from systemic infection, through mucocutaneous form, to vulvovaginal form. Although caused by one organism, each form is controlled by distinct host immune mechanisms. Phagocytosis by polymorphonuclea...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/BF02931313
更新日期：2007-01-01 00:00:00
abstract：:Treatment of Helicobacter pylori cells with several chaotropic agents resulted in different degrees of inhibition in the binding of the bacteria to hemin and Congo-red dye. Polyanions also yielded a >50% inhibitory effect. Furthermore, hydrophobic interaction chromatography was used to determine the relative surface h...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-011-0043-z
更新日期：2011-05-01 00:00:00
abstract：:A total of 40 substances were tested for their inhibitory effect on the multiplication of a bacteriophage in a growing culture of Bacillus licheniformis and their influence on bacitracin production. Acriflavine was the only substance which, at a concentration of 3 microgram ml-1, completely suppressed phage multiplica...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02876597
更新日期：1978-01-01 00:00:00
