Bioluminescence of Pseudomonas fluorescens HK44 in the course of encapsulation into silica gel. Effect of methanol.

Abstract:

:The bioluminescence (BLM) and colony-forming units (CFU) of Pseudomonas fluorescens HK44 were monitored during encapsulation into pre-polymerized Si(OMe)₄. The non-induced BLM of free cells was increased in the presence of 0.5-2.5 % MeOH. After mixing silica sol with the cell suspension, both BLM and CFU dropped to 1-3 and 8-18 %, respectively; both remained lowered as long as the silica biofilm contained residual MeOH. The kinetics of MeOH being released from silica biofilms (a thickness of 2-6 mm) were first-order. The decrease of bacterial activity due to encapsulation was proportional to the biofilm thickness. MeOH evolving during encapsulation is probably the principal stress factor but not the only one.

journal_title

Folia microbiologica

authors

Trögl J,Kuncová G,Kuráň P

doi

10.1007/s12223-010-0091-9

subject

Has Abstract

pub_date

2010-11-01 00:00:00

pages

569-75

issue

6

eissn

0015-5632

issn

1874-9356

journal_volume

55

pub_type

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