Abstract：
:Beauveria bassiana is widely studied as an alternative to chemical acaricides in controlling the cattle tick Rhipicephalus microplus. Although its biocontrol efficiency has been proved in laboratory and field scales, there is a need to a better understanding of host interaction process at molecular level related to biocontrol activity. In this work, applying a proteomic technique multidimensional protein identification technology (MudPIT), the differential secretome of B. bassiana induced by the host R. microplus cuticle was evaluated. The use of the host cuticle in a culture medium, mimicking an infection condition, is an established experimental model that triggers the secretion of inducible enzymes. From a total of 236 proteins, 50 proteins were identified exclusively in infection condition, assigned to different aspects of infection like host adhesion, cuticle penetration and fungal defense, and stress. Other 32 proteins were considered up- or down-regulated. In order to get a meaningful global view of the secretome, several bioinformatic analyses were performed. Regarding molecular function classification, the highest number of proteins in the differential secretome was assigned in to hydrolase activity, enzyme class of all cuticle-degrading enzymes like lipases and proteases. These activities were also further validated through enzymatic assays. The results presented here reveal dozens of specific proteins and different processes potentially implicated in cattle tick infection improving the understanding of molecular basis of biocontrol of B. bassiana against R. microplus.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Santi L,Coutinho-Rodrigues CJB,Berger M,Klein LAS,De Souza EM,Rosa RL,Guimarães JA,Yates JR 3rd,Perinotto WMS,Bittencourt VREP,Beys-da-Silva WOdoi
10.1007/s12223-018-0659-3subject
Has Abstractpub_date
2019-05-01 00:00:00pages
361-372issue
3eissn
0015-5632issn
1874-9356pii
10.1007/s12223-018-0659-3journal_volume
64pub_type
杂志文章abstract：:Fungal cells of Aureobasidium pullulans ATCC 201253 were immobilized by entrapment in chitosan beads, and the immobilized cells were investigated for their ability to produce the polysaccharide pullulan using batch fermentation. The 1% chitosan-entrapped fungal cells were capable of producing pullulan for two cycles o...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-011-0048-7
更新日期：2011-07-01 00:00:00
abstract：:The biological activity and the presence of genes sfp and ituD (surfactin and iturin A) among Bacillus strains isolated from the Amazon basin were determined. Bacillus spp. were tested for hemolytic activity and inhibition of fungal growth by agar plate assays in parallel with PCR for identification of sfp and ituD ge...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-011-0056-7
更新日期：2011-07-01 00:00:00
abstract：:Aminoglycosides at 2x or 4x minimum inhibitory concentration induced postantibiotic effects against Pseudomonas aeruginosa lasting 3.5-4.9 h (gentamicin) and 0.5-3.7 h (selemycin). Postantibiotic effects of subinhibitory concentrations of the aminoglycosides tested were substantially longer. Some combinations of supra...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814691
更新日期：1996-01-01 00:00:00
abstract：:A strain of Aspergillus giganteus cultivated in a medium with xylan produced two xylanases (xylanase I and II) which were purified to homogeneity. Their molar mass, estimated by SDS-PAGE, were 21 and 24 kDa, respectively. Both enzymes are glycoproteins with 50 degrees C temperature optimum; optimum pH was 6.0-6.5 for ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931639
更新日期：2004-01-01 00:00:00
abstract：:The ribosomal stalk is involved directly in the interaction of the elongation factors with the ribosome during protein synthesis. The stalk is formed by a complex of five proteins, four small acidic polypeptides and a larger protein which directly interacts with the rRNA at the GTPase center. In eukaryotes, the acidic...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/BF02816234
更新日期：1999-01-01 00:00:00
abstract：:A case report of ventriculoperitoneal shunt infection caused by Candida lusitaniae in a 6-year-old patient with cerebral astrocytoma and obstructive hydrocephalus is presented briefly with emphasis on the course of antifungal treatment. Seven isolates recovered subsequently from the cerebrospinal fluid were studied re...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-011-0063-8
更新日期：2011-09-01 00:00:00
abstract：:The effect of paraquat on the activities of antioxidant defense and detoxifying enzymes of the white-rot fungus Funalia trogii was determined. Paraquat increased the activities of glutathione reductase (GR), glutathione transferase (GT) and superoxide dismutase at 1 mmol/L, while at 0.1 mmol/L it did not affect the ac...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02818700
更新日期：2002-01-01 00:00:00
abstract：:Regulatory mechanism of primary metabolism responsible for the biosynthesis of peptide ergot alkaloids was proposed on the basis of experimental results from the production phase of Claviceps purpurea (FR.) Tul. in parasitic and saprophytic cultures. The production-phase metabolism is characterized by uncoupling of gl...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02927365
更新日期：1981-01-01 00:00:00
abstract：:Bacteria accumulate high amounts of potassium in the cytoplasm. For studying transport of K+ (with 86Rb as a marker) in bacteria (Staphylococcus aureus 17810S), the cells were depleted of the internal K+ pool by a DNP treatment. Kinetics and energetics of 86Rb transport was assayed with glucose as an exogenous energy ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02816378
更新日期：1998-01-01 00:00:00
abstract：:Altered cell signaling is the molecular basis for cell proliferation occurring in association with several gamma herpesvirus infections. Three gamma herpesviruses, namely EBV/HHV-4, KSHV/HHV-8 and the MHV-68 (and/or MHV-72) and their unusual cell-pirated gene products are discussed in this respect. The EBV, KSHV as we...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/BF02931360
更新日期：2003-01-01 00:00:00
abstract：:Enzyme synthesis of tryptophan from indole, pyruvate and ammonium salts was studied using Escherichia coli cells exhibiting a significant tryptophanase activity. In addition to the effect of cultivation medium composition and cultivation conditions, factors affecting the course of the conversion were investigated. Pro...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02820485
更新日期：1990-01-01 00:00:00
abstract：:Chemical and microscopic features of wood decay by the basidiomycete Coriolopsis occidentalis are described. The fungus was grown on blocks of poplar, oak, and fir wood and caused significant mass, lignin, and saccharide losses in all kinds of wood. Poplar wood was particularly strongly affected. Twelve weeks after in...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814527
更新日期：1994-01-01 00:00:00
abstract：:We constructed a SmaI genomic library of Cellulomonas biazotea DNA in E. coli and in the S. cerevisiae shuttle vector, YEP 24. Three clone were identified that conferred the ability for E. coli or S. cerevisiae transformants to produce carboxymethylcellulase (CMCase). Cells transformed with these clones were compared ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814307
更新日期：1994-01-01 00:00:00
abstract：:Pullulanase of Klebsiella oxytoca is one of a wide variety of extracellular proteins that are secreted by Gram-negative bacteria by the complex main terminal branch (MTB) of the general secretory pathway. The roles of some of the 14 components of the MTB are now becoming clear. In this review it is proposed that most ...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/BF02818976
更新日期：1997-01-01 00:00:00
abstract：:For the first time, it was reported in France a cluster of autochthonous severe community-acquired (CA) infections due to the USA300 methicillin-resistant Staphylococcus aureus (MRSA) clone. The three cases belonged to the same family without any identified clue of abroad importation pathway. The domestic spread of US...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-014-0322-6
更新日期：2014-11-01 00:00:00
abstract：:Isolation and structure elucidation was carried out of flavonoid constituents found in fractionated extracts of the seeds and green leaves of Daucus carota L. var. boissieri (Apiaceae). The flavonoids are mainly apigenin, luteolin, their glycosidic precursors and 2,4,5-trimethoxybenzaldehyde. Fatty acids, hydrocarbons...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02903720
更新日期：1999-01-01 00:00:00
abstract：:Mutations occurring in viral polymerase gene of hepatitis B virus (HBV) due to the use of nucleos(t)id analogs reduce the activity of the drugs by causing antiviral resistance. In this study, it was aimed to evaluate mutations responsible for drug resistance and drug resistance mutation rates in patients followed up b...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-018-0650-z
更新日期：2019-03-01 00:00:00
abstract：:The submerged culture of the Claviceps purpurea strain studied was polymorphous. In the process of alkaloid synthesis, cytodifferentiation preceded biochemical differentiation. The onset of the alkaloid phase was characterized by: predominance of chlamydospores in the culture, the presence of vegetative cells with red...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02876766
更新日期：1975-01-01 00:00:00
abstract：:The resistance to killing by free radicals of two mutants ofPhaffia rhodozyma was determined. Mutant 5-7 did not produce astaxanthin but produced beta-carotene, while mutant 3-4 did not produce any carotenoid pigments. The resistance of mutant 5-7 was the same as that of the wild type but mutant 3-4 was rapidly killed...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02816505
更新日期：1998-01-01 00:00:00
abstract：:Gut microbiome lives in the intestinal tract of animals and plays an important role in almost all life processes. Gut microbiome balance is beneficial to health, and imbalance leads to many diseases, one of which is obesity epidemic. However, gut microbiome is also influenced by host hormone, and different gut microbi...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-019-00704-4
更新日期：2019-11-01 00:00:00
abstract：:The geographical distribution of Bacillus anthracis strains and isolates bearing some of the same genetic markers as the Amerithrax Ames isolate was examined and evaluated. At least one mechanism for the horizontal movement of genetic markers was shown amongst isolates and closely related species and the effect of suc...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-008-0074-2
更新日期：2008-01-01 00:00:00
abstract：:Antibacterial effect of chitooligosaccharides (COS) and low molar mass chitosans (LMWC) is considered as one of the most important characteristics of chitosan (CS) hydrolysates. Here, we show the in vitro effect of different COS, LMWC, and CS on representative anaerobic bacteria isolated from human colon as a possibil...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-012-0138-1
更新日期：2012-07-01 00:00:00
abstract：:We present the results of analysis of membrane phosphoproteomes from individual morphological stages of Streptomyces coelicolor that reflect developmentally dependent heterogeneity and phosphorylation of intrinsic and externally added purified Strepomyces aureofaciens EF-Tu. Fast growing nonpathogenic Mycobacterium sm...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-010-0029-2
更新日期：2010-05-01 00:00:00
abstract：:A rifampicin-resistant variant of two strains of Lactobacillus plantarum, one strain of Pediococcus acidilactici, and one strain of Enterococcus faecium were used for the experimental production of lucerne silage. Laboratory silage without inoculants served as a control. Counts of total anaerobes, total lactic acid ba...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-012-0142-5
更新日期：2012-07-01 00:00:00
abstract：:Biochemical properties of a termostable alkaline phosphatase obtained from the mycelium extract of A. caespitosus were described. The enzyme was purified 42-fold with 32% recovery by DEAE-cellulose and concanavalin A-Sepharose chromatography. The molar mass estimated by Sephacryl S-200 or by 7% SDS-PAGE was 138 kDa an...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931303
更新日期：2007-01-01 00:00:00
abstract：:Infiltration of immune cells into CNS is one of the essential events in multiple sclerosis (MS) development. Adhesion molecules like the intercellular adhesion molecule 1 (ICAM-1) play critical role in this process. Therefore, the ICAM1 gene containing two important single-nucleotide polymorphisms (SNPs) belongs to ca...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-017-0499-6
更新日期：2017-07-01 00:00:00
abstract：:Candida bracarensis is an emerging cryptic species within the Candida glabrata clade. To date, little is known about its epidemiology, virulence, and antifungal susceptibility. This study documents the occurrence of C. bracarensis for the first time in Mexico and focuses on its in vitro production of hydrolytic enzyme...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-018-0592-5
更新日期：2018-07-01 00:00:00
abstract：:Thirty-three fully breast-fed infants aged between 1 and 12 weeks were screened for bifidobacteria in feces. Bifidobacteria counts in most fecal samples determined both by TPY agar and FISH procedure ranged from 10(8) to 10(11) CFU/g. Three infants did not contain any bifidobacteria in their fecal samples. One child w...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931404
更新日期：2004-01-01 00:00:00
abstract：:Lactobacillus bulgaricus 59 and L. casei RTS and their respective nitrosoguanidine-induced mutants were estimated for titratable and volatile acidities, production of biacetyl and acetoin and for proteolytic activity. Both the mutants showed a marked increase in proteolytic activity as compared to the parents. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02897216
更新日期：1980-01-01 00:00:00
abstract：:Mitochondria isolated from Neurospora crassa were purified by centrifugation in a Percoll density gradient. Enzyme activities and cytochrome differential spectra revealed a high purity of the mitochondria. As compared with a crude mitochondrial fraction the purified mitochondria exhibited a high respiratory activity a...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02879491
更新日期：1983-01-01 00:00:00
