Abstract：
:Competitive PCR method was developed for the detection and enumeration of Butyrivibrio fibrisolvens. Sequences of 16S rDNA were obtained from our isolates (serving as a source of data for primer design) and were distinguished into nine different groups of butyrivibria. Specific primers for two distinct groups were designed with the help of BioEdit program. These primers were tested with DNA of 20 strains of ruminal B. fibrisolvens isolates. Annealing temperature 58 degrees C showed a little specificity but a better selectivity was found after raising it up to 65 degrees C. A group 1 competitive fragment of 16S rDNA of different length was constructed using restriction cutting with MspI followed by ligation; the size of the resulting fragment was cut down by 75 bp. The fragment worked in the presence of the original 16S rDNA fragment of B. fibrisolvens JK 609.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Mrázek J,Kopecný Jdoi
10.1007/BF02825888keywords:
subject
Has Abstractpub_date
2001-01-01 00:00:00pages
63-5issue
1eissn
0015-5632issn
1874-9356journal_volume
46pub_type
杂志文章abstract：:Differences in the effect of volatile and gaseous metabolites of germinating pea seeds on the germination of spores of Mucor racemosus and macroconidia of Fusarium oxysporum are described. Germination of spores of M. racemosus was inhibited by seed metabolites whereas germination of macroconidia of F. oxysporum was st...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02933019
更新日期：1980-01-01 00:00:00
abstract：:One-week dextran sulfate treatment of conventional (CV) immunodeficient (SCID) mice gave rise to acute colitis in the colon mucosa; germ-free (GF) SCID mice did not exhibit any changes in colon morphology. Dextran sulfate application to CV immunocompetent (BALB/c) mice did induce substantial changes in the colon mucos...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02818004
更新日期：2001-01-01 00:00:00
abstract：:The diversity and structure of bacterial and actinobacteral diversity and communities were determined in a metallophytic grassland soil from an upland site in northern England. The community profiles were subjected to multivariate analyses using correspondence and cluster analyses. The total bacterial community divers...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-009-0042-5
更新日期：2009-01-01 00:00:00
abstract：:Streptomyces aureofaciens glucosidizes 1,2,4-trihydroxy-9,10-anthraquinone (purpurin) added to the cultivation medium to yield the corresponding 2-beta-D-glucoside. The identity of the glucoside was demonstrated by comparing its physico-chemical properties with data of an authentic sample prepared synthetically. A fur...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02873156
更新日期：1984-01-01 00:00:00
abstract：:The levels of anti-Candida antibodies were determined in experimental animals immunized with 4 different yeast doses (0.3, 0.6, 1.2, 2.4 x 10(8) CFU) at weekly intervals. After immunization (sampling intervals 1, 2, and 3 months), the intravenous blood was examined for the presence of serum anti-Candida antibodies. Af...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931340
更新日期：2003-01-01 00:00:00
abstract：:The reserves of fossil-based fuels, which currently seem sufficient to meet the global demands, is inevitably on the verge of exhaustion. Contemporary raw material for alternate fuel like biodiesel is usually edible plant commodity oils, whose increasing public consumption rate raises the need of finding a non-edible ...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/s12223-018-0602-7
更新日期：2018-09-01 00:00:00
abstract：:In this report, Gluconobacter strains were screened for coenzyme Q10 (CoQ10) production. A thermotolerant strain, Gluconobacter japonicus FM10, was eventually employed for CoQ10 production optimization. To do so, a two-step optimization strategy was used. The first step focused on biomass increase and the second step ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-018-0666-4
更新日期：2019-07-01 00:00:00
abstract：:Dithiocarbamates were found to enhance the production of citric acid under solid-state fermentation conditions by Aspergillus niger. Maximum increase was observed with tetramethylthiuram disulfide (TMTD). Percent increases observed were 74.2% with 2.5 micrograms/mL of TMTD, 19.6% with 2.5 micrograms/mL of sodium dimet...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02926953
更新日期：1986-01-01 00:00:00
abstract：:The synthesis of exocellular proteinase decreases with increasing concentration of amino acids in the growth medium. After removal of amino acids the enzyme synthesis is gradually restored to normal values. The presence of inhibitors of transcription (actinomycin D) or translation (chloramphenicol) blocks the restorat...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02875957
更新日期：1984-01-01 00:00:00
abstract：:Isolates of Stenotrophomonas maltophilia species display the feature "temperature-dependent susceptibility" (TDS) to antibiotics. Both 30TDS strains (at least 4 times lower value of minimum inhibitory concentration (MIC) of an antibiotic at 30 than at 37 degrees C) and 37TDS strains (at least 4 times lower value of MI...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02818682
更新日期：2002-01-01 00:00:00
abstract：:Glycerol-based medium (BM) with and without the addition of 1 g/L ascorbic acid (Asc) and/or 100 mg/L (+/-)-catechin (Cat) was tested for the storage of three commercial wine yeasts at -20 degrees C. The medium supplemented with Asc was also used to store 706 strains to verify the maintenance of the liquid state. A de...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-009-0036-3
更新日期：2009-01-01 00:00:00
abstract：:For the first time, it was reported in France a cluster of autochthonous severe community-acquired (CA) infections due to the USA300 methicillin-resistant Staphylococcus aureus (MRSA) clone. The three cases belonged to the same family without any identified clue of abroad importation pathway. The domestic spread of US...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-014-0322-6
更新日期：2014-11-01 00:00:00
abstract：:The mathematical theory of substrate-induced acidification by microorganisms was based on special formulation of mass and charge conservation laws for distinct species. The strength of the theory was tested on the ethanol-induced acidification by Candida utilis in pure water and in phthalate buffer. We showed that the...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02836618
更新日期：1992-01-01 00:00:00
abstract：:The antifungal effect of 2-alkylthio-6-amino- and 2-alkylthio-6-formamidobenzothiazoles (22 derivatives in all) on Aspergillus niger and various Candida yeasts was tested. No significant effect was observed with A. niger. With the pathogenic yeast species C. albicans and C. guilliermondii, the most efficient derivativ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814461
更新日期：1989-01-01 00:00:00
abstract：:The addition of a nitrogen (nitrate) and carbon sources (acetate, citrate and fructose) and phosphate deficiency (nitrate medium deficient in phosphate) under unaerated conditions induced akinete differentiation in Anabaena torulosa. Aerated cultures of this organism in these nutrients did not differentiate akinetes. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02817617
更新日期：2000-01-01 00:00:00
abstract：:The assumption that the pyruvate decarboxylase activity of Saccharomyces carlsbergensis is the main limiting factor determining the formation rate and the total amount of d(--)-l-hydroxy-l-phenyl-propanone (phenylacetylcarbinol, PAC) produced was not confirmed. An increase of about 30% of the total amount of the PAC p...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02877396
更新日期：1982-01-01 00:00:00
abstract：:The effect of dental plaque bacteria on LFA-1 beta expression on peripheral blood leukocytes was studied in 20 patients with early-onset periodontitis and in 10 healthy controls. Stimulation of PMN with selected dental plaque bacteria which play a role in the pathogeny of periodontitis significantly increased the expr...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02815697
更新日期：1996-01-01 00:00:00
abstract：:Treatment of Helicobacter pylori cells with several chaotropic agents resulted in different degrees of inhibition in the binding of the bacteria to hemin and Congo-red dye. Polyanions also yielded a >50% inhibitory effect. Furthermore, hydrophobic interaction chromatography was used to determine the relative surface h...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-011-0043-z
更新日期：2011-05-01 00:00:00
abstract：:Bacillus subtilis strains expressing listeriolysin O (LLO) and simultaneously LLO and p60 protein were constructed. The effect of p60 protein on hemolytic activity and on the invasion of professional phagocytes was demonstrated in the absence of other virulence factors of L. monocytogenes. The hemolytic activity of LL...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931440
更新日期：2005-01-01 00:00:00
abstract：:The temperature dependence of fluorescence anisotropy, lifetime and differential tangent of 1,6-diphenyl-1,3,5-hexatriene (DPH) and its polar trimethylammonium derivative (TMA-DPH) were investigated in cytoplasmic membranes of Bacillus subtilis. The fluorescence parameters were compared in the two types of membranes p...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02821406
更新日期：1990-01-01 00:00:00
abstract：:Phytoplankton successions under ice cover (January-March) were determined in four oligotrophic lakes (Burtjärn, Aspen, Vialamptjärn and Storkorstjärn) located in North-Eastern Sweden. The total phosphorus concentration in the lakes was less than 10 micrograms/L. Lake Burtjärn (reference lake) had a similar hydrology a...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931370
更新日期：2003-01-01 00:00:00
abstract：:Vancomycin is often used in orthopedic surgery as a local prophylaxis of bacterial infection. The aim of this work was to compare the release of vancomycin and its biologically inactive crystalline degradation products (CDP-1) during in vitro experiments from different types of local antibiotic delivery systems (bone ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-019-00752-w
更新日期：2020-06-01 00:00:00
abstract：:Ten thermophilic Gram-positive bacteria were isolated from various soils of Saudi Arabia. The strains are spore-forming rods belonging to the species Bacillus stearothermophilus. The cells are motile, strictly aerobic, catalase and oxidase positive. The sporangia appear to be swollen and their position varies from ter...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02927328
更新日期：1981-01-01 00:00:00
abstract：:Studies on the production of extracellular cellulase in two aquatic hyphomycetes Lunulospora curvula and Flagellospora penicillioides have shown that several factors such as carbon source, nitrogen source, pH and temperature affect the production of the enzyme. Experiments have shown that carboxymethyl cellulose is th...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814357
更新日期：1991-01-01 00:00:00
abstract：:A 6.5 kb DNA fragment containing the gene (thrC) encoding threonine synthase, the last enzyme of the threonine biosynthetic pathway, has been cloned from the DNA of Bacillus sp. ULM1 by complementation of Escherichia coli and Brevibacterium lactofermentum thrC auxotrophs. Complementation studies showed that the thrB g...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02818515
更新日期：1995-01-01 00:00:00
abstract：:Raoultella spp. representatives are Gram-negative capsulated, nonmotile rods. These bacteria are found in the natural environment: plants, water, soil and insects. R. ornithinolytica is one of the three species of Raoultella. R. ornithinolytica is the only species within the genus which has the ability to produce orni...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-015-0390-2
更新日期：2015-11-01 00:00:00
abstract：:The emergence of spa types and spa-clonal complexes (CC) among clinical methicillin-resistant Staphylococcus aureus isolates collected from the University Clinical Center in Gdańsk between 2008 and 2009 were investigated. Phage typing was used as the initial screening in the study. The basic set of phages and the addi...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-012-0148-z
更新日期：2012-05-01 00:00:00
abstract：:Stabilized sewage sludge is applied to agricultural fields and farmland due to its high organic matter content. The aim of this study was to investigate the effects of two types of sludge stabilization, mesophilic anaerobic digestion (MAD) and thermophilic anaerobic digestion (TAD), on bacterial communities in sludge,...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-015-0396-9
更新日期：2015-11-01 00:00:00
abstract：:Pseudomonas aeruginosa is an opportunistic pathogen that causes serious infections, especially in patients with immunodeficiency. It exhibits multiple mechanisms of resistance, including efflux pumps, antibiotic modifying enzymes and limited membrane permeability. The primary reason for the development of novel therap...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-014-0333-3
更新日期：2015-01-01 00:00:00
abstract：:Inducible pigmentation changes were observed in pigmented strains of Brevibacterium sp. M27 and B. flavum treated with N-methyl-N'-nitro-N-nitrosoguanidine. The highest frequency of induction was reached already at a survival of 30-40% with the maximal yield of 6-10%. As compared with the initial yellow colour, three ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02928007
更新日期：1988-01-01 00:00:00
