Abstract：
:A 6.5 kb DNA fragment containing the gene (thrC) encoding threonine synthase, the last enzyme of the threonine biosynthetic pathway, has been cloned from the DNA of Bacillus sp. ULM1 by complementation of Escherichia coli and Brevibacterium lactofermentum thrC auxotrophs. Complementation studies showed that the thrB gene (encoding homoserine kinase) is found downstream from the thrC gene, and analysis of nucleotide sequences indicated that the hom gene (encoding homoserine dehydrogenase) is located upstream of the thrC gene. The organization of this cluster of genes is similar to the Bacillus subtilis threonine operon (hom-thrC-thrB). An 1.9 kb BclI fragment from the Bacillus sp. ULM1 DNA insert 351 amino acids was found corresponding to a protein of 37462 Da. The thrC gene showed a low G + C content (39.4%) and the encoded threonine synthase is very similar to the B. subtilis enzyme. Expression of the 1.9 kb BcI DNA fragment in E. coli minicells resulted in the formation of a 37 kDa protein. The upstream region of this gene shows promoter activity in E. coli but not in corynebacteria. A peptide sequence, including a lysine that is known to bind the pyridoxal phosphate cofactor, is conserved in all threonine synthase sequences and also in the threonine and serine dehydratase genes. Amino acid comparison of nine threonine synthases revealed evolutionary relationships between different groups of bacteria.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Malumbres M,Mateos LM,Guerrero C,Martín JFdoi
10.1007/BF02818515subject
Has Abstractpub_date
1995-01-01 00:00:00pages
595-606issue
6eissn
0015-5632issn
1874-9356journal_volume
40pub_type
杂志文章abstract：:The aim of our study was to find out the optimal conditions for short-term storage of cerebrospinal fluid (CSF) samples for direct diagnosis of Lyme disease. A mixture of Borrelia-negative CSFs spiked with a defined amount of cultured Borrelia garinii was used. Borrelia stability was investigated over 7 days at four d...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-015-0408-9
更新日期：2016-01-01 00:00:00
abstract：:Lengths of permuted regions in the P22 and L phage genomes were estimated from the relative yields of DNA in many electrophoretic bands obtained using several restriction endonucleases. It was found that 3.6 kb (8.7%) of P22-DNA and 7.2 kb (17.8%) of L-DNA were circularly permuted. In both phages the sequential packag...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02933145
更新日期：1992-01-01 00:00:00
abstract：:Isolation and structure elucidation was carried out of flavonoid constituents found in fractionated extracts of the seeds and green leaves of Daucus carota L. var. boissieri (Apiaceae). The flavonoids are mainly apigenin, luteolin, their glycosidic precursors and 2,4,5-trimethoxybenzaldehyde. Fatty acids, hydrocarbons...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02903720
更新日期：1999-01-01 00:00:00
abstract：:Cord and newborn sera were found to possess a significantly decreased in vitro bactericidal activity to Escherichia coli as compared to sera of adult individuals. Hyperbilirubinaemia in neonates suffering from both Rh and ABO incompatibility results in a further significant impairment of the bactericidal activity of t...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02927298
更新日期：1979-01-01 00:00:00
abstract：:The chloramphenicol resistance gene from pSa was introduced into T-DNA of pTi T37 of Agrobacterium tumefaciens by cointegration with intermediary plasmid based on pBR322. The resulting intermediary vector was mobilized to A. tumefaciens T37 by conjugative plasmid pRK2. The RK2 plasmid also forms contegrates with pTi d...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02926824
更新日期：1986-01-01 00:00:00
abstract：:Acmycin, a new polyene antibiotic, was fungistatic at low and fungicidal at high concentrations. It produced malformations in conidia of Helminthosporium oryzae and Curvularia lunata and caused leakage of yeast cells. Acmycin was fairly nontoxic to germinating seeds. The intravenous LD50 was 300-400 and intraperitonea...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02879559
更新日期：1983-01-01 00:00:00
abstract：:Diseases of the central nervous system (CNS) mean for the human organism a potentially dangerous situation. An investigation of cerebrospinal fluid (CSF) provides important information about a character of CNS impairment in the decision-making diagnostic and therapeutic algorithm. The authors present a brief overview ...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/s12223-018-0663-7
更新日期：2019-05-01 00:00:00
abstract：:Aminoglycosides at 2x or 4x minimum inhibitory concentration induced postantibiotic effects against Pseudomonas aeruginosa lasting 3.5-4.9 h (gentamicin) and 0.5-3.7 h (selemycin). Postantibiotic effects of subinhibitory concentrations of the aminoglycosides tested were substantially longer. Some combinations of supra...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814691
更新日期：1996-01-01 00:00:00
abstract：:The validity of the principle of homeoviscous adaptation for Bacillus subtilis was tested by comparing fluorescence anisotropy (1,6-diphenyl-1,3,5-hexatriene) and electron-spin resonance (16-doxylstearate) measurements carried out in isolated plasma membranes and in phospholipid fractions. The physical measurements we...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02925901
更新日期：1988-01-01 00:00:00
abstract：:Inducible pigmentation changes were observed in pigmented strains of Brevibacterium sp. M27 and B. flavum treated with N-methyl-N'-nitro-N-nitrosoguanidine. The highest frequency of induction was reached already at a survival of 30-40% with the maximal yield of 6-10%. As compared with the initial yellow colour, three ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02928007
更新日期：1988-01-01 00:00:00
abstract：:When studying the kinetics of DNA synthesis, growth and cell division in Escherichia coli B/r after irradiation with different doses of UV-radiation (254 nm) we could demonstrate, by means of pulse incorporation of 3H-thymidine, a lag in DNA synthesis after the irradiation. The relative rate of the restored DNA synthe...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02876446
更新日期：1982-01-01 00:00:00
abstract：:Numerous antibodies with a known mechanism of action are utilized as possible means for studying morphogenesis and differentiation. Inhibitors of biosynthesis of nucleic acids and proteins, compounds intervening with the synthesis and/or function of cell walls and membranes or compounds influencing the energy metaboli...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/BF02897219
更新日期：1980-01-01 00:00:00
abstract：:Overpopulation of domestic pigeons is considered to be one of the major problems of urban centers, as these birds are responsible for the dissemination of relevant pathogens to animal and human health. The aim of this study was to detect potentially pathogenic Escherichia coli and Salmonella spp. in domestic pigeons c...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-020-00781-w
更新日期：2020-08-01 00:00:00
abstract：:Raoultella spp. representatives are Gram-negative capsulated, nonmotile rods. These bacteria are found in the natural environment: plants, water, soil and insects. R. ornithinolytica is one of the three species of Raoultella. R. ornithinolytica is the only species within the genus which has the ability to produce orni...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-015-0390-2
更新日期：2015-11-01 00:00:00
abstract：:Cryptosporidiosis belongs to the important parasitic infections with zoonotic potential and the occurrence in European countries is rare. The first cases of cryptosporidiosis caused by Cryptosporidium hominis detected in the Slovak republic were described here. Collection of examined humans consisted of five family me...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-012-0182-x
更新日期：2013-01-01 00:00:00
abstract：:Biofilms represent mixed communities present in a diverse range of environments; however, their utility as inoculants is less investigated. Our investigation was aimed towards in vitro development of biofilms using fungal mycelia (Trichoderma viride) as matrices and nitrogen-fixing and P-solubilizing bacteria as partn...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-012-0154-1
更新日期：2012-09-01 00:00:00
abstract：:The sorption ability of Candida utilis biomass for cadmium ions with accumulating competence of dried cells and cells in alginate was compared. After an optimization of process conditions (pH 5.5, biomass concentration 1 g/L and c0 50 mg/L), the cadmium sorption capacity of dried yeast biomass was perceptibly higher t...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931807
更新日期：2006-01-01 00:00:00
abstract：:The geographical distribution of Bacillus anthracis strains and isolates bearing some of the same genetic markers as the Amerithrax Ames isolate was examined and evaluated. At least one mechanism for the horizontal movement of genetic markers was shown amongst isolates and closely related species and the effect of suc...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-008-0074-2
更新日期：2008-01-01 00:00:00
abstract：:The ability of Phanerochaete chrysosporium, Trametes versicolor, Coriolopsis polyzona, and Pleurotus ostreatus growing in a mitogen-limited mineral medium (NMM) to degrade PCBs in a commercial, Delor 106 mixture at a concentration of 0.9 ppm was compared. The respective amount of PCBs removed from the fungal cultures ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02898723
更新日期：1997-01-01 00:00:00
abstract：:Bacteria accumulate high amounts of potassium in the cytoplasm. For studying transport of K+ (with 86Rb as a marker) in bacteria (Staphylococcus aureus 17810S), the cells were depleted of the internal K+ pool by a DNP treatment. Kinetics and energetics of 86Rb transport was assayed with glucose as an exogenous energy ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02816378
更新日期：1998-01-01 00:00:00
abstract：:The screening of 17 SAg genes of S. aureus isolated from the sputum of cystic fibrosis (CF) patients revealed that among 47 genetically different strains, 39 (83 %) carried SAg genes. Superantigens forming enterotoxin gene cluster were detected in 20 strains. The 2nd most common superantigen type was selk detected in ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931628
更新日期：2006-01-01 00:00:00
abstract：:Effects of increasing doses of UV-B radiation on photosynthetic efficiency (PE) and motility in Chlamydomonas reinhardtii were investigated. The exposure time ranged from 10 to 120 min at 0.73 W/m2 UV-B radiation. A decrease in compensation points (CP) was followed by an increase in PE on extending the UV-B treatment ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02817448
更新日期：2000-01-01 00:00:00
abstract：:The effect of immersion time and cell concentration in the attachment of several lactic acid bacteria with antibacterial activity to beef-muscle surface was studied. The number of firmly attached bacteria increased with immersion time in the case of Pediococcus acidilacti, Lactobacillus sake, Lactococcus cremoris (two...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814710
更新日期：1996-01-01 00:00:00
abstract：:The ability of surfactants obtained from three Lactobacillus acidophilus strains to inhibit Staphylococcus aureus and S. epidermidis biofilms was evaluated. Their influence was determined on bacterial initial adhesion, biofilm formation and dispersal using MTT-reduction assay, confocal laser scanning microscopy and im...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-008-0009-y
更新日期：2008-01-01 00:00:00
abstract：:Ecosystems worldwide are exposed to pollutants connected to the industrial production of pharmaceuticals. The objective of this study was to study the composition and characteristics of the soil microbial communities that had been exposed to long-term selection pressure caused by the industrial production of penicilli...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-020-00819-z
更新日期：2020-12-01 00:00:00
abstract：:A proteus strain inhibited mycelial growth of Fusarium oxysporum in vitro. Seed bacterization showed significant plant growth promotion and Fusarium-wilt suppression activity of Phaseolus mungo in a gnotobiotic system. The culture filtrate of this strain exhibited three prominent bands in UV-VIS spectra between 300 an...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02818723
更新日期：2000-01-01 00:00:00
abstract：:Some Bacillus subtilis mutants with different levels of homoserine dehydrogenase were described. Strains that do not accumulate methionine have a high homoserine dehydrogenase activity. Low activity was detected in mutants where cell growth was completely inhibited by 0.7 mmol/L methionine. A low concentration of dime...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02884064
更新日期：1991-01-01 00:00:00
abstract：:The uvrE (E. coli KS 114) cells carry a mutation in the gene that codes for helicase II. This is the protein responsible for replicative unwinding of double-helical DNA. The repair mode of such cells may be altered as compared with the wild type. The survival of uvrE cells during postirradiation incubation under inhib...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02922491
更新日期：1985-01-01 00:00:00
abstract：:Fibrobacter succinogenes is a major cellulolytic species in the rumen. On the basis of molecular data, this species can be classified into four phylogenetic groups. Recently gathered ecological and physiological data have revealed the importance of this species, particularly phylogenetic group 1, in rumen fiber digest...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/s12223-008-0024-z
更新日期：2008-01-01 00:00:00
abstract：:We present the results of analysis of membrane phosphoproteomes from individual morphological stages of Streptomyces coelicolor that reflect developmentally dependent heterogeneity and phosphorylation of intrinsic and externally added purified Strepomyces aureofaciens EF-Tu. Fast growing nonpathogenic Mycobacterium sm...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-010-0029-2
更新日期：2010-05-01 00:00:00
