DNA synthesis in Escherichia coli B/r after UV-irradiation.

Abstract:

:When studying the kinetics of DNA synthesis, growth and cell division in Escherichia coli B/r after irradiation with different doses of UV-radiation (254 nm) we could demonstrate, by means of pulse incorporation of 3H-thymidine, a lag in DNA synthesis after the irradiation. The relative rate of the restored DNA synthesis (related to the number of viable cells) was higher than in the non-irradiated culture. After 3 h the rate of DNA synthesis settled at a constant value, which was identical with the control rate up to the "critical dose" of 20 J/m2. The irradiated cell population is heterogenous and contains basically two categories of cells--surviving and non-surviving. Cells of both types contribute to DNA synthesis restored after the lag period to a different extent. During the first hour after the irradiation even the nonviable portion of the population, i.e. cells that do not form colonies but are still penicillin-sensitive, is involved in the DNA synthesis.

journal_title

Folia microbiologica

authors

Kucerová H,Vĕchet B

doi

10.1007/BF02876446

subject

Has Abstract

pub_date

1982-01-01 00:00:00

pages

370-6

issue

6

eissn

0015-5632

issn

1874-9356

journal_volume

27

pub_type

杂志文章
  • The detection of anti-Candida antibodies on experimental animal models.

    abstract::The levels of anti-Candida antibodies were determined in experimental animals immunized with 4 different yeast doses (0.3, 0.6, 1.2, 2.4 x 10(8) CFU) at weekly intervals. After immunization (sampling intervals 1, 2, and 3 months), the intravenous blood was examined for the presence of serum anti-Candida antibodies. Af...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02931340

    authors: Pilipcinec E,Dorko E,Tkáciková L,Wantrubová A,Bugarský A

    更新日期:2003-01-01 00:00:00

  • A Raman-spectroscopy-based approach for detection and discrimination of Streptococcus thermophilus and Lactobacillus bulgaricus phages at low titer in raw milk.

    abstract::In this study, a method combining Raman spectroscopy with chemometric analysis was developed for detection of phage presence in raw milk and discrimination of Streptococcus thermophilus and Lactobacillus bulgaricus phages which are among the main phages causing problems in dairy industry. For this purpose, S. thermoph...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/s12223-018-0604-5

    authors: Tayyarcan EK,Acar Soykut E,Boyaci IH

    更新日期:2018-09-01 00:00:00

  • Determination of minimal regrowth concentration (MRC) in clinical isolates of various biofilm-forming bacteria.

    abstract::Based on the ability to attach to polymeric surfaces, the formation of biofilms was determined in 5 wild-type strains (Pseudomonas aeruginosa, Klebsiella pneumoniae, Acinetobacter baumanii, Escherichia coli, Staphylococcus warneri). Using modified Christensen method, minimum regrowth concentration (MRC) of piperacilli...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02931650

    authors: Cernohorská L,Votava M

    更新日期:2004-01-01 00:00:00

  • Formation of extracellular neutral proteinase and the stringent response in Bacillus subtilis.

    abstract::The kinetics of extracellular neutral proteinase synthesis by an isogenic stringent (IS58) and a relaxed (IS56) strain of B. subtilis were compared. The specific enzyme formation rate by the stringent strain was higher than that of the relaxed one. Norvaline addition (1 mg/mL) induced the formation of pppGpp and ppGpp...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02883234

    authors: Riedel K,Schroeter A,Liebs P,Graba JP,Hecker M,Schrapel D

    更新日期:1987-01-01 00:00:00

  • Semicontinuous cultivation of autotrophic algae.

    abstract::Mathematical expressions were derived for the dilution rate and concentration of algae determining the maximum biomass production in a semicontinuous algal culture. ...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02926655

    authors: Lívanský K

    更新日期:1979-01-01 00:00:00

  • The use of antibiotics for studies of morphogenesis and differentiation in microorganisms.

    abstract::Numerous antibodies with a known mechanism of action are utilized as possible means for studying morphogenesis and differentiation. Inhibitors of biosynthesis of nucleic acids and proteins, compounds intervening with the synthesis and/or function of cell walls and membranes or compounds influencing the energy metaboli...

    journal_title:Folia microbiologica

    pub_type: 杂志文章,评审

    doi:10.1007/BF02897219

    authors: Betina V

    更新日期:1980-01-01 00:00:00

  • The effect of length of incubation on adherence of group B streptococci to epithelial cells.

    abstract::Adhesion of group B streptococci to human epithelial vaginal and buccal cells proceeded in three phases which differed qualitatively. Maximum adhesion took place within 10 min of interaction, during the second phase (10-15 min), the percentage of adherent cells decreased significantly (P less than 0.05) whereas during...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02884088

    authors: Kubín V,Jirásková Z,Franĕk J

    更新日期:1983-01-01 00:00:00

  • Phenolic acids reduce the genotoxicity of acridine orange and ofloxacin in Salmonella typhimurium.

    abstract::Naturally occurring plant phenolics, p-coumaric acid (PA), caffeic acid (CA), ferulic acid (FA) and gentisic acid (GA) (25-100 nmol/L) had protective effects on acridine orange (AO; 216 mumol/L)- and ofloxacin (3 mumol/L)-induced genotoxicity in Salmonella typhimurium. FA, GA and CA exhibited a significant concentrati...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02817994

    authors: Belicová A,Krizková L,Nagy M,Krajcovic J,Ebringer L

    更新日期:2001-01-01 00:00:00

  • Survival of and wheat-root colonization by alginate encapsulated Herbaspirillum spp.

    abstract::The survival of Herbaspirillum spp. cells added directly or encapsulated in alginate beads and colonization of wheat roots was evaluated in soil microcosms. Cells entrapped in alginate in the presence of JNFb-broth and introduced into unplanted non-sterile clay loamy and sandy soils survived better than cells added di...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02825879

    authors: el-Komy HM

    更新日期:2001-01-01 00:00:00

  • Phenomenological theory of substrate-induced acidification with application to Candida utilis dissimilating ethanol.

    abstract::The mathematical theory of substrate-induced acidification by microorganisms was based on special formulation of mass and charge conservation laws for distinct species. The strength of the theory was tested on the ethanol-induced acidification by Candida utilis in pure water and in phthalate buffer. We showed that the...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02836618

    authors: Votruba J,Páca J

    更新日期:1992-01-01 00:00:00

  • Phenotypic and genotypic approach to characterize Arcanobacterium pluranimalium isolated from bovine milk samples.

    abstract::In the present study, three Arcanobacterium pluranimalium strains isolated from bovine milk samples of three cows of three farms (two cows with subclinical mastitis) could successfully be identified by phenotypical investigations, by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-T...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/s12223-016-0450-2

    authors: Wickhorst JP,Hassan AA,Sammra O,Huber-Schlenstedt R,Lämmler C,Prenger-Berninghoff E,Timke M,Abdulmawjood A

    更新日期:2016-09-01 00:00:00

  • Effects of temperature and novobiocin on the expression of calf prochymosin gene and on plasmid copy number in recombinant Escherichia coli.

    abstract::Escherichia coli strain HB101 harboring an expression plasmid bearing calf prochymosin gene under the control of the tac promoter was grown in the presence of IPTG with or without novobiocin at 28 and 40 degrees C, respectively. The differential rates of synthesis of prochymosin inclusions, and, for comparison, of bet...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02815545

    authors: Kaprálek F,Tichý PJ,Fábry M,Sedlácek J

    更新日期:1998-01-01 00:00:00

  • Detection of Shiga toxins, intimin and enterohemolysin in Escherichia coli strains isolated from children in eastern Slovakia.

    abstract::Fifty Escherichia coli strains isolated from stool samples of 51 healthy children, 143 strains isolated from stool samples of 327 children with diarrhea and 24 strains isolated from stool samples of 21 children with suspected hemolytic uremic syndrome were examined for the presence of Shiga toxin-producing E. coli vir...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02817680

    authors: Liptáková A,Siegfried L,Podracká L,Sabol M,Sehnálková H,Bogyiová E,Rosocha J,Kmetová M,Kerestesová H,Kotulová D

    更新日期:2002-01-01 00:00:00

  • Effects of increasing doses of UV-B radiation on photosynthesis and motility in Chlamydomonas reinhardtii.

    abstract::Effects of increasing doses of UV-B radiation on photosynthetic efficiency (PE) and motility in Chlamydomonas reinhardtii were investigated. The exposure time ranged from 10 to 120 min at 0.73 W/m2 UV-B radiation. A decrease in compensation points (CP) was followed by an increase in PE on extending the UV-B treatment ...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02817448

    authors: Danilov RA,Ekelund NG

    更新日期:2000-01-01 00:00:00

  • Some properties of Streptococcus thermophilus bacteriophages.

    abstract::The morphology, host range, structural proteins and serological properties of Streptococcus thermophilus phages isolated from Finnish cheese plants were investigated. The results show that all the nine phages belong morphologically to Ackermann's group B1. The host-phage reactions and plating efficiency justify the di...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02883235

    authors: Kivi S,Peltomäki T,Luomala K,Sarimo SS

    更新日期:1987-01-01 00:00:00

  • Phenol-water extracts of gram-positive Listeria monocytogenes and gram-negative Salmonella typhimurium. Comparison of biological activities.

    abstract::Using phenol--water extraction, a lipopeptidopolysaccharide complex (LPPS) was isolated from Listeria monocytogenes. Some biological and immunological properties of LPPS were compared with lipopolysaccharide isolated by the same procedure from Salmonella typhimurium. LPPS possesses low pyrogenicity, but the immunologi...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02923515

    authors: Hofman J,Pospísil M,Mára M,Hríbalová V

    更新日期:1985-01-01 00:00:00

  • Biotransformation of 1,8-cineole by solid-state fermentation of Eucalyptus waste from the essential oil industry using Pleurotus ostreatus and Favolus tenuiculus.

    abstract::Biotechnological conversion of low-cost agro-industrial by-products, such as industrial waste or terpenes from the distillation of essential oils from plants into more valuable oxygenated derivatives, can be achieved by using microbial cells or enzymes. In Argentina, the essential oil industry produces several tons of...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/s12223-015-0422-y

    authors: Omarini A,Dambolena JS,Lucini E,Jaramillo Mejía S,Albertó E,Zygadlo JA

    更新日期:2016-03-01 00:00:00

  • Introducing a thermotolerant Gluconobacter japonicus strain, potentially useful for coenzyme Q10 production.

    abstract::In this report, Gluconobacter strains were screened for coenzyme Q10 (CoQ10) production. A thermotolerant strain, Gluconobacter japonicus FM10, was eventually employed for CoQ10 production optimization. To do so, a two-step optimization strategy was used. The first step focused on biomass increase and the second step ...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/s12223-018-0666-4

    authors: Moghadami F,Fooladi J,Hosseini R

    更新日期:2019-07-01 00:00:00

  • Effective of Bacterial repair systems in near UV radiation-induced damage.

    abstract::Inactivation of seven strains derived from Escherichia coli B differing in their capacity to repair damage to their DNA (exc, pol, rec) after irradiation with far (254 nm) and middle and near (300 to 380 and 320--400 nm) UV light was investigated. The same bacterial strains were also used as hosts for the UV-irradiate...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02876690

    authors: Janovská E,Samoilova KA,Vízdalová M

    更新日期:1980-01-01 00:00:00

  • Pharmacodynamic parameters of aminoglycosides and their effect on exoenzymes of Pseudomonas aeruginosa.

    abstract::Aminoglycosides at 2x or 4x minimum inhibitory concentration induced postantibiotic effects against Pseudomonas aeruginosa lasting 3.5-4.9 h (gentamicin) and 0.5-3.7 h (selemycin). Postantibiotic effects of subinhibitory concentrations of the aminoglycosides tested were substantially longer. Some combinations of supra...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02814691

    authors: Hostacká A

    更新日期:1996-01-01 00:00:00

  • Genotyping and PCR detection of potential virulence genes in Campylobacter jejuni and Campylobacter coli isolates from different sources in Poland.

    abstract::The prevalence of potential virulence markers was determined among the population of Polish Campylobacter jejuni and Campylobacter coli isolates from children, chickens, pigs and dogs. The presence of the flaA, flaB, cdtA, cdtB, cdtC, cdtABC, virB11, and cj0588 genes among 74 C. jejuni and 15 C. coli isolates was dete...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/s12223-010-0025-6

    authors: Krutkiewicz A,Klimuszko D

    更新日期:2010-03-01 00:00:00

  • Expression of the LFA-1 beta molecule on peripheral blood leukocytes of patients with early-onset periodontitis: effects of dental plaque microbes.

    abstract::The effect of dental plaque bacteria on LFA-1 beta expression on peripheral blood leukocytes was studied in 20 patients with early-onset periodontitis and in 10 healthy controls. Stimulation of PMN with selected dental plaque bacteria which play a role in the pathogeny of periodontitis significantly increased the expr...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02815697

    authors: Procházková J,Bártová J,Bílková A,Kruzík P,Krejsa O,Dusková J,Mrklas L

    更新日期:1996-01-01 00:00:00

  • Biological properties of acmycin, a new polyene antibiotic.

    abstract::Acmycin, a new polyene antibiotic, was fungistatic at low and fungicidal at high concentrations. It produced malformations in conidia of Helminthosporium oryzae and Curvularia lunata and caused leakage of yeast cells. Acmycin was fairly nontoxic to germinating seeds. The intravenous LD50 was 300-400 and intraperitonea...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02879559

    authors: Chakrabarti S,Chandra AL

    更新日期:1983-01-01 00:00:00

  • Intracellular colonization of Rhododendron and Vaccinium roots by Cenococcum geophilum, Geomyces pannorum and Meliniomyces variabilis.

    abstract::Four in vitro experiments were set up to verify the colonization potential of ectomycorrhizal (EcM) Cenococcum geophilum FR. (strain CGE-4), saprotrophic Geomyces pannorum (LINK) SIGLER & CARMICHAEL (GPA-1) and a frequent root-associated, potentially ericoid mycorrhiza (ErM)-forming Meliniomyces variabilis Hambleton &...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02932096

    authors: Vohník M,Fendrych M,Albrechtová J,Vosátka M

    更新日期:2007-01-01 00:00:00

  • Anaerobic ammonium-oxidizing bacteria in river water treatment wetland.

    abstract::Anaerobic ammonium-oxidizing (anammox) bacteria play an essential part in nitrogen removal in constructed wetlands. The objective of the present study was to explore the spatiotemporal dynamics of anammox bacterial communities and the associated factors in a full-scale constructed wetland for the treatment of polluted...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/s12223-019-00726-y

    authors: Tong T,Li B,Xie S

    更新日期:2020-04-01 00:00:00

  • Rapid G+ count and subpopulation assessment of the intestinal bacteria in Apodemus sylvaticus and Mus musculus by flow cytometry.

    abstract::We report a novel application of calcein-acetomethyl ester in flow cytometry for rapid estimation of the number of G+-bacteria in rodent feces (Apodemus sylvaticus and Mus sp.f. muridae). We also use the combined application of flow cytometry and Syto-13 or Sypro Orange staining to count rapidly the total bacterial po...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02931538

    authors: Moragues M,Comas-Riu J,Vives-Rego J

    更新日期:2004-01-01 00:00:00

  • Assessment of a bacteriocin-producing Lactobacillus strain in the control of spoilage of a cereal-based African fermented food.

    abstract::As a part of a program to develop starter cultures aiding in the spoilage control and sanitation of African fermented foods, a cereal-based food ('ogi' and its solid form 'agidi' or 'eko') was prepared using a bacteriocin-producing Lactobacillus strain as the starter culture. The survival of an enterotoxigenic Escheri...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02898642

    authors: Olasupo NA,Olukoya DK,Odunfa SA

    更新日期:1997-01-01 00:00:00

  • Effect of dithiocarbamates on citric acid production by Aspergillus niger.

    abstract::Dithiocarbamates were found to enhance the production of citric acid under solid-state fermentation conditions by Aspergillus niger. Maximum increase was observed with tetramethylthiuram disulfide (TMTD). Percent increases observed were 74.2% with 2.5 micrograms/mL of TMTD, 19.6% with 2.5 micrograms/mL of sodium dimet...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02926953

    authors: Khanna V,Gupta KG

    更新日期:1986-01-01 00:00:00

  • Alpha,beta-unsaturated 4-oxo acids in heterocylic synthesis. II. Behavior of 3-(1,1-dioxadibenzothein-4-oyl)acrylic acid towards carbon nucleophiles under Michael reaction condition.

    abstract::3-(1,1-Dioxadibenzothien-4-oyl)acrylic acid (1) was condensed with compounds containing active methylene groups under Michael reaction conditions to furnish the Michael adducts (lactones 2a-c, lactams 3a-c, ketones 4a,b). The behavior of these adducts toward the action of hydrazine hydrate were investigated. The compo...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02817670

    authors: Wasfy AA,Amine MS,Arief MM,Donia SG,Aly AA

    更新日期:2002-01-01 00:00:00

  • Structure and function of the stalk, a putative regulatory element of the yeast ribosome. Role of stalk protein phosphorylation.

    abstract::The ribosomal stalk is involved directly in the interaction of the elongation factors with the ribosome during protein synthesis. The stalk is formed by a complex of five proteins, four small acidic polypeptides and a larger protein which directly interacts with the rRNA at the GTPase center. In eukaryotes, the acidic...

    journal_title:Folia microbiologica

    pub_type: 杂志文章,评审

    doi:10.1007/BF02816234

    authors: Rodriguez-Gabriel MA,Bou G,Briones E,Zambrano R,Remacha M,Ballesta JP

    更新日期:1999-01-01 00:00:00