Abstract:
:Streptomyces caelestis DSM 40084 produces two osmolytes, viz. 2-O-(alpha-D-glucopyranosyl)-zeta-glyceric acid (GG) and trehalose. Both compounds were isolated and identified by nuclear magnetic resonance spectroscopy and mass spectrometry. A very sensitive regulation of the cell osmolytes was demonstrated in exponentially growing cultures. The intracellular levels of GG and trehalose increased 2x in response to a step change of medium osmolarity caused by 0.3% NaCl. 1H NMR analysis of the cell extracts did not confirm the presence of additional osmolytes. GG is a S. caelestis metabolite commonly released from the cells; its concentration reached 3 g/L during the cultivation in a yeast extract--(NH4)2SO4-glycerol medium. This is the first report on the occurrence of the ionic osmolyte GG in the genus Streptomyces and on its free excretion to the medium.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Pospísl S,Halada P,Petrícek M,Sedmera Pdoi
10.1007/BF02932103subject
Has Abstractpub_date
2007-01-01 00:00:00pages
451-6issue
5eissn
0015-5632issn
1874-9356journal_volume
52pub_type
杂志文章abstract::Dithiocarbamates were found to enhance the production of citric acid under solid-state fermentation conditions by Aspergillus niger. Maximum increase was observed with tetramethylthiuram disulfide (TMTD). Percent increases observed were 74.2% with 2.5 micrograms/mL of TMTD, 19.6% with 2.5 micrograms/mL of sodium dimet...
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journal_title:Folia microbiologica
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journal_title:Folia microbiologica
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