Glucosylglycerate is an osmotic solute and an extracellular metabolite produced by Streptomyces caelestis.

Abstract:

:Streptomyces caelestis DSM 40084 produces two osmolytes, viz. 2-O-(alpha-D-glucopyranosyl)-zeta-glyceric acid (GG) and trehalose. Both compounds were isolated and identified by nuclear magnetic resonance spectroscopy and mass spectrometry. A very sensitive regulation of the cell osmolytes was demonstrated in exponentially growing cultures. The intracellular levels of GG and trehalose increased 2x in response to a step change of medium osmolarity caused by 0.3% NaCl. 1H NMR analysis of the cell extracts did not confirm the presence of additional osmolytes. GG is a S. caelestis metabolite commonly released from the cells; its concentration reached 3 g/L during the cultivation in a yeast extract--(NH4)2SO4-glycerol medium. This is the first report on the occurrence of the ionic osmolyte GG in the genus Streptomyces and on its free excretion to the medium.

journal_title

Folia microbiologica

authors

Pospísl S,Halada P,Petrícek M,Sedmera P

doi

10.1007/BF02932103

subject

Has Abstract

pub_date

2007-01-01 00:00:00

pages

451-6

issue

5

eissn

0015-5632

issn

1874-9356

journal_volume

52

pub_type

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