Abstract:
:Ultrastructural changes induced by Rickettsia slovaca standard type (ST) and wild type (WT) were examined during their life cycle in L929 and Vero cells. R. slovaca invaded the cytoplasm of the host cell by phagocytosis on the 1st d p.i. Rickettsiae adhering to the cytoplasmic membrane were engulfed by cellular extensions and occurred in phagocytic vacuoles. Binary fission of rickettsia was observed. The nuclear chromatin of eukaryotic cells was rearranged and condensed during 3rd and 6th d p.i. Finally, loss of the plasma membrane integrity, destruction of cytoplasm and nucleus resulted in cell lysis. Degeneration of the host cell caused by WT and ST was observed after 4 and 5 d p.i. in L929 cells and after 3 and 6 d p.i. in Vero cells, respectively. WT type was able to penetrate into the nucleus of the host cell and was responsible for dilatation of the perinuclear space and endoplasmic reticulum, causing more pronounced and different cytopathological changes than the ST.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Boldis V,Strus J,Kocianová E,Tusek-Znidaric M,Stefanidesová K,Spitalská Edoi
10.1007/s12223-009-0019-4subject
Has Abstractpub_date
2009-01-01 00:00:00pages
130-6issue
2eissn
0015-5632issn
1874-9356journal_volume
54pub_type
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