Abstract:
:The present study was designed to characterize phenotypically and genotypically a Trueperella pyogenes strain isolated from a brain abscess of an adult roebuck (Capreolus capreolus). The species identity could be confirmed by phenotypical investigations, by MALDI-TOF MS analysis, and by sequencing the 16S ribosomal RNA (rRNA) gene, the 16S-23S rRNA intergenic spacer region (ISR); by sequencing the target genes rpoB, gap, and tuf; and by detection of T. pyogenes chaperonin-encoding gene cpn60 with a previously developed loop-mediated isothermal amplification (LAMP) assay. The T. pyogenes strain could additionally be characterized by PCR-mediated amplification of several known and putative virulence factor-encoding genes which revealed the presence of the genes plo encoding pyolysin and nanH and nanP encoding neuraminidases; the genes fimA, fimC, and fimE encoding the fimbrial subunits FimA, FimC, and FimE; and the gene cbpA encoding collagen-binding protein CbpA. The present data give a detailed characterization of a T. pyogenes strain isolated from a brain abscess of a roebuck. However, the route of infection of the roebuck remains unclear.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Wickhorst JP,Hassan AA,Sheet OH,Eisenberg T,Sammra O,Alssahen M,Lämmler C,Prenger-Berninghoff E,Zschöck M,Timke M,Abdulmawjood Adoi
10.1007/s12223-017-0533-8subject
Has Abstractpub_date
2018-01-01 00:00:00pages
17-22issue
1eissn
0015-5632issn
1874-9356pii
10.1007/s12223-017-0533-8journal_volume
63pub_type
杂志文章abstract::Data on the production of clostridial toxins in dialyzed cultures are summarized. Principal modifications of this cultivation technique suitable for both research and production are shown. If toxins are released from the cells by autolysis (neurotoxins of Clostridium tetani, C. botulinum; lethal factors of C. novyi an...
journal_title:Folia microbiologica
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journal_title:Folia microbiologica
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journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02814710
更新日期:1996-01-01 00:00:00
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journal_title:Folia microbiologica
pub_type: 杂志文章
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更新日期:2005-01-01 00:00:00
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pub_type: 杂志文章,评审
doi:10.1007/BF02925852
更新日期:1988-01-01 00:00:00
abstract::Using fluorescein-labelled antibodies against gamma, mu and alpha chains, Ig-containing cells in palatine tonsils were studied in 120 patients. The aim of this study was to determine the most frequently repeated typical findings as regards the numbers and localisation of these cells in tonsils and to confront the data...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02927432
更新日期:1981-01-01 00:00:00
abstract::Regulatory mechanism of primary metabolism responsible for the biosynthesis of peptide ergot alkaloids was proposed on the basis of experimental results from the production phase of Claviceps purpurea (FR.) Tul. in parasitic and saprophytic cultures. The production-phase metabolism is characterized by uncoupling of gl...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02927365
更新日期:1981-01-01 00:00:00
abstract::Substitution with NO2, Cl, Br and I into position 6 of 2-benzthiazolthiol derivatives increased their antimicrobial efficiency while the SH group is preserved. This increase affected both G+ and G--bacteria, mycobacteria and some fungi. The degree of efficiency of the benzthiazol derivatives is also influenced by the ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02876583
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doi:10.1007/BF02876690
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abstract::A case report of ventriculoperitoneal shunt infection caused by Candida lusitaniae in a 6-year-old patient with cerebral astrocytoma and obstructive hydrocephalus is presented briefly with emphasis on the course of antifungal treatment. Seven isolates recovered subsequently from the cerebrospinal fluid were studied re...
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abstract::We constructed a SmaI genomic library of Cellulomonas biazotea DNA in E. coli and in the S. cerevisiae shuttle vector, YEP 24. Three clone were identified that conferred the ability for E. coli or S. cerevisiae transformants to produce carboxymethylcellulase (CMCase). Cells transformed with these clones were compared ...
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abstract::Cardiolipin synthesis was studied during the aerobic synchronous growth of aerobically grown yeast Saccharomyces cerevisiae. The time course of the synthesis was stepwise and the rise in cardiolipin level in cells coincided in time with the increase in cytochrome oxidase activity. This finding supports the notion of d...
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