Abstract:
:Secreted aspartyl proteinase (Sap) distribution among different C. albicans isolates was determined using SAP-specific primers in polymerase chain reaction (PCR) assay. SAP1, SAP2, and SAP3 were detected in 13 of 40 (32.5%), SAP4 in 38/40 (95%), SAP5 were detected in 30/40 (75%), SAP6 in 23/40 (57.5%) of C. albicans strains isolated from blood cultures. SAP1-SAP3 were detected in 37 of 40 (92.5%), SAP4 were detected in 3/40 (7.5%), SAP5 in 3/40 (7.5%), SAP6 in 5/40 (12.5%) of C. albicans strains isolated from vaginal swab cultures. Sap1, Sap2 and Sap3 isoenzymes were found to be related to the vaginopathic potential of C. albicans; Sap4, Sap5 and Sap6 isoenzymes were found to be correlated with systemic infections.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Kalkanci A,Bozdayi G,Biri A,Kustimur Sdoi
10.1007/BF02931422keywords:
subject
Has Abstractpub_date
2005-01-01 00:00:00pages
409-13issue
5eissn
0015-5632issn
1874-9356journal_volume
50pub_type
杂志文章abstract::Three types of heterogeneous preparations and four types of preparations of polysaccharide nature were obtained in studies aimed at the isolation of active compounds from Aspergillus flavus conidia bearing their biological stimulatory activity. Extraction with trichloroacetic acid at 0 degrees C yielded a preparation ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02927425
更新日期:1981-01-01 00:00:00
abstract::A hexokinase preparation was obtained from a Saccharomyces cerevisiae mutant strain deficient in glucosephosphate isomerase (GPI) and mannosephosphate isomerase (MPI) by precipitation with ammonium sulfate. The supernatant fraction corresponding to 40-60 % saturation showed the lowest content in GPI and MPI activity. ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02927364
更新日期:1981-01-01 00:00:00
abstract::A total of 20 putative strains of Erwinia amylovora originating from 11 samples of host plants with symptoms of fire blight were analyzed in detail using commercial polyclonal antibodies in immunochemical tests. Fourteen strains reacted negatively in all tests; 6 strains reacted positively with a polyclonal antibody f...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931569
更新日期:2005-01-01 00:00:00
abstract::Contemporary aspects of cerebrospinal fluid analysis are discussed, including the relationship to neuro-infective, autoimmune and other neurological diseases. The actual state of cerebrospinal fluid microbiological and cytological investigation and analysis of cerebrospinal fluid protein fractions are described in det...
journal_title:Folia microbiologica
pub_type: 杂志文章,评审
doi:10.1007/BF02931522
更新日期:2003-01-01 00:00:00
abstract::Biological activity of secondary metabolites produced by a plant-growth-promoting Pseudomonas fluorescens was evaluated. The strain produced antibiotics phenazine (PHE), 2,4-diacetylphloroglucinol (PHL) and siderophore pyoverdin (PYO) in standard King's B and succinic acid media, respectively. After extraction, PYO wa...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02818690
更新日期:2002-01-01 00:00:00
abstract::A new simple method used to eliminate polysaccharides that cause problems during DNA isolation was established for 6 different white-rot fungi using 1% hexadecyltrimethylammonium bromide (CTAB) as wash buffer and followed by centrifugation. Variation in the DNA yield and quality was ascertained using precipitating age...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931383
更新日期:2004-01-01 00:00:00
abstract::Composition of sterol fractions of nystatin-resistant Candida maltosa strains was determined. Using UV-spectrometry, TLC and GLC-MS it was demonstrated that resistance to nystatin is connected with the composition alterations of yeast cell sterols. Block of different stages of ergosterol biosynthesis was revealed in s...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02814494
更新日期:1991-01-01 00:00:00
abstract::The biochemical basis of a defective bioenergetic system was attempted to be determined in N,N'-dicyclohexylcarbodiimide (DCCD)-resistant mutant of Methanothermobacter thermautotrophicus. Components participating in the maintenance of methanoarchaeal membrane structure and function, such as the composition of the mixt...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-008-0034-x
更新日期:2008-01-01 00:00:00
abstract::The chloramphenicol resistance gene from pSa was introduced into T-DNA of pTi T37 of Agrobacterium tumefaciens by cointegration with intermediary plasmid based on pBR322. The resulting intermediary vector was mobilized to A. tumefaciens T37 by conjugative plasmid pRK2. The RK2 plasmid also forms contegrates with pTi d...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02926824
更新日期:1986-01-01 00:00:00
abstract::Addition of glucose to a resting cell suspension of the yeast Saccharomyces cerevisiae was accompanied by marked shifts of the G alpha-protein subunits from the plasma membrane to the cell interior. This process was rapid with half-times between < 10 and 20 s. The decrease of the plasma membrane pool of the Gi alpha/G...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02814427
更新日期:2001-01-01 00:00:00
abstract::The highest level of secreted brefeldin A was present in glucose-grown cultures, intermediate levels in glucose-fructose, and xylose cultures and low levels in fructose- and galactose-grown cultures of Curvularia lunata. The biomass decreased from glucose, fructose, xylose, glucose-fructose to galactose cultures. Bref...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02876993
更新日期:1977-01-01 00:00:00
abstract::We isolated and identified functional groups of bacteria in the rumen of Creole goats involved in ruminal fermentation of native forage shrubs. The functional bacterial groups were evaluated by comparing the total viable, total anaerobic, cellulolytic, hemicellulolytic, and amylolytic bacterial counts in the samples t...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-012-0219-1
更新日期:2013-09-01 00:00:00
abstract::Enzyme synthesis of tryptophan from indole, pyruvate and ammonium salts was studied using Escherichia coli cells exhibiting a significant tryptophanase activity. In addition to the effect of cultivation medium composition and cultivation conditions, factors affecting the course of the conversion were investigated. Pro...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02820485
更新日期:1990-01-01 00:00:00
abstract::Staphylococcus epidermidis is a biofilm-forming bacterial strain that can cause major problems as an agent of nosocomial infections. Bacteria in biofilms are shielded from the environment and can survive high doses of antibiotics. We here test the antibiotic susceptibility of Staphylococcus epidermidis to rising genta...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-017-0568-x
更新日期:2018-05-01 00:00:00
abstract::Using phenol--water extraction, a lipopeptidopolysaccharide complex (LPPS) was isolated from Listeria monocytogenes. Some biological and immunological properties of LPPS were compared with lipopolysaccharide isolated by the same procedure from Salmonella typhimurium. LPPS possesses low pyrogenicity, but the immunologi...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02923515
更新日期:1985-01-01 00:00:00
abstract::Using an in vitro cytotoxicity assay (51Cr-release), it was shown that bilirubin exerts a cytotoxic effect on both adult and newborn human lymphocytes after a 1-d incubation of cells with bilirubin. The effect of bilirubin on human granulocytes was less pronounced; the association of the cytotoxic effect with a functi...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02923520
更新日期:1985-01-01 00:00:00
abstract::The prevalence of potential virulence markers was determined among the population of Polish Campylobacter jejuni and Campylobacter coli isolates from children, chickens, pigs and dogs. The presence of the flaA, flaB, cdtA, cdtB, cdtC, cdtABC, virB11, and cj0588 genes among 74 C. jejuni and 15 C. coli isolates was dete...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-010-0025-6
更新日期:2010-03-01 00:00:00
abstract::Lactobacillus pobuzihii is a novel species which has been previously found in pobuzihi (fermented cummingcordia), a traditional fermented food in Taiwan. However, the lactic acid bacteria (LAB) microflora in pobuzihi has not been studied in detail. In this study, LAB from pobuzihi were isolated, identified, and charac...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-012-0188-4
更新日期:2013-03-01 00:00:00
abstract::The plasmid R1drd-19 Km- and its derivative pON5300 were split by the PstI restriction enzyme; they differed in a 2.4 Mg/mol fragment which was present only in the pON5300. Both plasmids were present in 1-2 copies per chromosomal equivalent in E. coli JC5455 cells. The pON5300 is stable in its original mutagenized hos...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02879554
更新日期:1983-01-01 00:00:00
abstract::Diseases of the central nervous system (CNS) mean for the human organism a potentially dangerous situation. An investigation of cerebrospinal fluid (CSF) provides important information about a character of CNS impairment in the decision-making diagnostic and therapeutic algorithm. The authors present a brief overview ...
journal_title:Folia microbiologica
pub_type: 杂志文章,评审
doi:10.1007/s12223-018-0663-7
更新日期:2019-05-01 00:00:00
abstract::Sixty four biopsy specimens of oral Candida leukoplakias were examined. Histological sections were stained with periodic acid-Schiff (PAS) reagent and by Grocott's silver method. C. albicans isolates were cultivated from all the patients but fungal hyphae were proved histologically only in 23 of them. PAS-positive and...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02814437
更新日期:2001-01-01 00:00:00
abstract::The uvrE (E. coli KS 114) cells carry a mutation in the gene that codes for helicase II. This is the protein responsible for replicative unwinding of double-helical DNA. The repair mode of such cells may be altered as compared with the wild type. The survival of uvrE cells during postirradiation incubation under inhib...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02922491
更新日期:1985-01-01 00:00:00
abstract::The root nodules of Phaseolus mungo (a herbaceous leguminous pulse) contained a high amount of 3-indolylacetic acid (IAA). A tryptophan pool present in the nodule might play the role of precursor for IAA production. From the root nodule a Rhizobium sp. was isolated. The symbiont produced a large amount of IAA (142 mic...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-008-0054-6
更新日期:2008-01-01 00:00:00
abstract::A method is presented for the successful decontamination of sparingly soluble pteridine derivatives by microwave irradiation. The method is nondestructive, rapid and effective in eliminating contamination. ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02814514
更新日期:1991-01-01 00:00:00
abstract::Virulence factors were compared in 15 Aeromonas spp. isolated from faeces of patients with Aeromonas-associated gastroenteritis and in 81 strains isolated from food. Strains from food did not show differences in the distribution of virulence factors when compared with strains isolated from faeces. However, 88.8% of Ae...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02814323
更新日期:1994-01-01 00:00:00
abstract::Inhibition activity of the alkaloid (-)-corypalmine on spore germination of plant pathogenic and saprophytic fungi (Alternaria solani, A brassicicola, A. brassicae, A. melongenae, Curvularia pallescens, C. lunata, C. maculans, Curvularisa sp., Colletotrichum sp., Helminthosporium speciferum, H. frumentacei, H. pennise...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02817654
更新日期:2002-01-01 00:00:00
abstract::Candida bracarensis is an emerging cryptic species within the Candida glabrata clade. To date, little is known about its epidemiology, virulence, and antifungal susceptibility. This study documents the occurrence of C. bracarensis for the first time in Mexico and focuses on its in vitro production of hydrolytic enzyme...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-018-0592-5
更新日期:2018-07-01 00:00:00
abstract::Biochemical properties of a termostable alkaline phosphatase obtained from the mycelium extract of A. caespitosus were described. The enzyme was purified 42-fold with 32% recovery by DEAE-cellulose and concanavalin A-Sepharose chromatography. The molar mass estimated by Sephacryl S-200 or by 7% SDS-PAGE was 138 kDa an...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931303
更新日期:2007-01-01 00:00:00
abstract::The temperature dependence of fluorescence anisotropy, lifetime and differential tangent of 1,6-diphenyl-1,3,5-hexatriene (DPH) and its polar trimethylammonium derivative (TMA-DPH) were investigated in cytoplasmic membranes of Bacillus subtilis. The fluorescence parameters were compared in the two types of membranes p...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02821406
更新日期:1990-01-01 00:00:00
abstract::In the present study, the cDNA sequences of Hsp70 and Hsp90 genes of Isaria farinosa (designated IFHSP70 and IFHSP90) were cloned and characterized using multiple techniques of molecular biology and bioinformatics. The genetic differentiation of the two genes was investigated among 10 geographically separated populati...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-018-00676-x
更新日期:2019-07-01 00:00:00