Abstract:
:The temperature dependence of fluorescence anisotropy, lifetime and differential tangent of 1,6-diphenyl-1,3,5-hexatriene (DPH) and its polar trimethylammonium derivative (TMA-DPH) were investigated in cytoplasmic membranes of Bacillus subtilis. The fluorescence parameters were compared in the two types of membranes prepared from bacteria cultivated at 20 and 40 degrees C. Steady-state anisotropy measurements showed that within a broad range of temperatures, membranes cultivated at 20 degrees C exhibit significantly lower values than those prepared from cells cultivated at 40 degrees C. The temperature dependence of lifetime and differential tangent measurements (differential polarized phase fluorimetry) were fully consistent with steady-state anisotropy data of both DPH and TMA-DPH. The low anisotropy values in the case of TMA-DPH could be explained by a shorter lifetime and higher temperature-induced decrease as compared with DPH. Surprisingly, the temperature dependence of rotational rate R calculated according to the model of hindered rotations (Lakowicz 1983) gave misleading results. When increasing the temperature from 5 to 25 degrees C, a marked drop of rotational relaxation rate was observed. The minimum R values were measured between 25 and 30 degrees C and further increase of temperature (up to 60 degrees C) was reflected as increase of the R values. Therefore, a new model of "heterogeneous rotations" was developed. This model assumes that even at low temperatures (approaching 0 degrees C) where the differential tangent reaches zero, a fraction of fast rotating molecules exists. The ratio between fast and slowly rotating molecules may be expressed by this model, the newly calculated rotational rates are fully consistent with anisotropy, lifetime and differential tangent measurements and represent the monotonically increasing function of temperature.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Konopásek I,Svobodová J,Toptygin DD,Svoboda Pdoi
10.1007/BF02821406subject
Has Abstractpub_date
1990-01-01 00:00:00pages
371-83issue
5eissn
0015-5632issn
1874-9356journal_volume
35pub_type
杂志文章abstract::We report a novel application of calcein-acetomethyl ester in flow cytometry for rapid estimation of the number of G+-bacteria in rodent feces (Apodemus sylvaticus and Mus sp.f. muridae). We also use the combined application of flow cytometry and Syto-13 or Sypro Orange staining to count rapidly the total bacterial po...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931538
更新日期:2004-01-01 00:00:00
abstract::The emergence of spa types and spa-clonal complexes (CC) among clinical methicillin-resistant Staphylococcus aureus isolates collected from the University Clinical Center in Gdańsk between 2008 and 2009 were investigated. Phage typing was used as the initial screening in the study. The basic set of phages and the addi...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-012-0148-z
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journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02820804
更新日期:1998-01-01 00:00:00
abstract::Biological activity of secondary metabolites produced by a plant-growth-promoting Pseudomonas fluorescens was evaluated. The strain produced antibiotics phenazine (PHE), 2,4-diacetylphloroglucinol (PHL) and siderophore pyoverdin (PYO) in standard King's B and succinic acid media, respectively. After extraction, PYO wa...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02818690
更新日期:2002-01-01 00:00:00
abstract::Streptococcus dysgalactiae subsp. equisimilis (SDSE), belonging to the group C and G streptococci, are human pathogens reported to cause clinical manifestations similar to infections caused by Streptococcus pyogenes. To scrutinize the distribution of gene coding for S. pyogenes virulence factors in SDSE, 255 isolates ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-018-0595-2
更新日期:2018-09-01 00:00:00
abstract::Five trisubstituted quinazolones and eight trisubstituted quinazoline-4-thiones have been tested for antibacterial effects by a microdilution method. Four derivatives exerted a significant effect on E. coli, P. aeruginosa, S. aureus and B. subtilis (IC50 < 100 mg/L). In the bacterium P. aeruginosa six quinazolines sho...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02817411
更新日期:2000-01-01 00:00:00
abstract::Mathematical expressions were derived for the dilution rate and concentration of algae determining the maximum biomass production in a semicontinuous algal culture. ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02926655
更新日期:1979-01-01 00:00:00
abstract::Investigation of the dependence of cell yield of Candida boidinii in a chemostat and specific growth rate in a turbidostat on temperature was carried out using methanol as substrate. Each of the curves obtained had only one maximum, the optimum temperature for yield (Ys) and for specific growth rate (micron) being som...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02885600
更新日期:1977-01-01 00:00:00
abstract::In the present study, 193 Aspergillus strains were isolated from a total of 100 soil samples of pistachio orchards, which all of them were identified as Aspergillus flavus as the most abundant species of Aspergillus section Flavi existing in the environment. Approximately 59%, 81%, and 61% of the isolates were capable...
journal_title:Folia microbiologica
pub_type: 杂志文章
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更新日期:2012-01-01 00:00:00
abstract::Fifty Escherichia coli strains isolated from stool samples of 51 healthy children, 143 strains isolated from stool samples of 327 children with diarrhea and 24 strains isolated from stool samples of 21 children with suspected hemolytic uremic syndrome were examined for the presence of Shiga toxin-producing E. coli vir...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02817680
更新日期:2002-01-01 00:00:00
abstract::Candida bracarensis is an emerging cryptic species within the Candida glabrata clade. To date, little is known about its epidemiology, virulence, and antifungal susceptibility. This study documents the occurrence of C. bracarensis for the first time in Mexico and focuses on its in vitro production of hydrolytic enzyme...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-018-0592-5
更新日期:2018-07-01 00:00:00
abstract::In the original publication, the number of the NCTC strain used was published incorrectly. The authors apologize for the error. The error has no effect on the validity of the results and is corrected for accuracy. ...
journal_title:Folia microbiologica
pub_type: 杂志文章,已发布勘误
doi:10.1007/s12223-017-0558-z
更新日期:2018-01-01 00:00:00
abstract::The induction of tryptophanase was not affected by rifampicin in the rifampicin-resistant mutant of Vibrio el tor while the antibiotic inhibited the induction of tryptophanase in the normal strain at level of ribonucleic acid and protein synthesis. ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02877677
更新日期:1977-01-01 00:00:00
abstract::Chemical and microscopic features of wood decay by the basidiomycete Coriolopsis occidentalis are described. The fungus was grown on blocks of poplar, oak, and fir wood and caused significant mass, lignin, and saccharide losses in all kinds of wood. Poplar wood was particularly strongly affected. Twelve weeks after in...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02814527
更新日期:1994-01-01 00:00:00
abstract::A high infective dose of Taxocara canis eggs (2,500 eggs per mouse) induced a partial immunosuppression in mice, manifested by inhibition of the proliferative response of splenic T and B cells to polyclonal activators. A glucan immunomodulator given to infected animals at the beginning of the experiment showed a marke...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02820794
更新日期:1998-01-01 00:00:00
abstract::The effect of dental plaque bacteria on LFA-1 beta expression on peripheral blood leukocytes was studied in 20 patients with early-onset periodontitis and in 10 healthy controls. Stimulation of PMN with selected dental plaque bacteria which play a role in the pathogeny of periodontitis significantly increased the expr...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02815697
更新日期:1996-01-01 00:00:00
abstract::Fibrobacter succinogenes is a major cellulolytic species in the rumen. On the basis of molecular data, this species can be classified into four phylogenetic groups. Recently gathered ecological and physiological data have revealed the importance of this species, particularly phylogenetic group 1, in rumen fiber digest...
journal_title:Folia microbiologica
pub_type: 杂志文章,评审
doi:10.1007/s12223-008-0024-z
更新日期:2008-01-01 00:00:00
abstract::Resistance determinants for HgCl2 and CdCl2 were eliminated along with a number of antibiotic resistance factors from Acinetobacter lwoffi and Micrococcus sp. at 44 degrees C. These organisms were orginally resistant to HgCl2, merbromin, CdCl2, Pb(NO3)2, benzylpenicillin, erythromycin, carbenicillin, tetracycline and ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02925907
更新日期:1988-01-01 00:00:00
abstract::Genetic diversity of 89 isolates of Rhizoctonia solani isolated from different pulse crops representing 21 states from 16 agro-ecological regions of India, 49 morphological, and 7 anastomosis groups (AGs) was analyzed using 12 universal rice primers (URPs), 22 random amplified polymorphic DNA (RAPD), and 23 inter-simp...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-012-0165-y
更新日期:2012-11-01 00:00:00
abstract::The aim of this study was to determine the prevalence of Listeria sp. and Listeria monocytogenes in soil samples with reference to type of fertilizers (natural and artificial) and distance from places intensively exploited by men, as well as to determine the relationship between the presence of L. monocytogenes in the...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-013-0260-8
更新日期:2014-01-01 00:00:00
abstract::The majority of antibiotics and substances with diverse biological activity used in medicine are produced by actinomycetes, nonfilamentous bacteria and fungi. Other microorganisms, such as myxobacteria, pseudomonads, nocardias, basidiomycetes, marine microorganisms, enterobacteria, halobacteria, hyperthermophiles etc....
journal_title:Folia microbiologica
pub_type: 杂志文章,评审
doi:10.1007/BF02993460
更新日期:2003-01-01 00:00:00
abstract::Mycobacterium avium paratuberculosis (Map) is a pathogen which causes a chronic progressive granulomatous enteritis known as paratuberculosis or Johne's disease and it primarily affects wild and domestic ruminants. The aim of this research was to examine a flock which consisted of 294 goats and was located in Garfagna...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-017-0518-7
更新日期:2017-11-01 00:00:00
abstract::Extrusion of metabolites (glycerol, lactic, malic, and succinic acid) during the medium acidification caused by resting baker's yeast supplied with 200 mM glucose was studied under aerobic and anaerobic conditions and in the absence and presence of 14 mM KCl. The maximum levels of glycerol and of the sum of acids (abo...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02876611
更新日期:1980-01-01 00:00:00
abstract::Substitution with NO2, Cl, Br and I into position 6 of 2-benzthiazolthiol derivatives increased their antimicrobial efficiency while the SH group is preserved. This increase affected both G+ and G--bacteria, mycobacteria and some fungi. The degree of efficiency of the benzthiazol derivatives is also influenced by the ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02876583
更新日期:1978-01-01 00:00:00
abstract::A case report of ventriculoperitoneal shunt infection caused by Candida lusitaniae in a 6-year-old patient with cerebral astrocytoma and obstructive hydrocephalus is presented briefly with emphasis on the course of antifungal treatment. Seven isolates recovered subsequently from the cerebrospinal fluid were studied re...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-011-0063-8
更新日期:2011-09-01 00:00:00
abstract::Three methods were used for the isolation of fungi in the present investigation: the dilution-plate method, hair-baiting technique with horse hairs and the "exposed plate" method. Sabouraud's glucose agar at 28 degrees C was also used as isolation medium. 102 species and 2 species varieties belonging to 36 genera were...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02821282
更新日期:1990-01-01 00:00:00
abstract::The purpose of this study was to isolate bioluminescent strains and to phenotypically and biochemically identify them based on the 16S rRNA gene sequence. 16S rRNA gene sequence analysis of the 11 isolates revealed that they belonged to Shewanella woodyi. Nevertheless, they were determined to exhibit various growth ch...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-013-0269-z
更新日期:2014-01-01 00:00:00
abstract::A naturally deficient thiamine and methionine requiring strain of Bacillus coagulans (Ms 5) accumulates lysine in medium only when exogenous pyridoxine (optimal concentration, 0.1 mu g/ml) are supplied. Threonine exerts an inhibitory effect at higher concentrations but pyridoxine does not. ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02877085
更新日期:1975-01-01 00:00:00
abstract::Oxygen uptake by the spores of Fusarium moniliforme, F. oxysporum, F. semitectum, F. solani, Mucor racemosus and Trichoderma viride was increased in the presence of volatile substances extracted from Origanum majorana and Ocimum basilicum. This increase was greater in the presence of volatile substances from O. basili...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02885580
更新日期:1978-01-01 00:00:00
abstract::The possibility of utilizing derivatives of 4-methylumbelliferone (4-MUBF) for the determination of activity of enzymes from the group of hydrolyses was tested in 20 strains of 12 species of mycobacteria. The method proved to be suitable especially for determination of arylsulphatase, beta-D-galactose and acid phospha...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02876698
更新日期:1980-01-01 00:00:00