Abstract：
:Activity of purified alanylaminopeptidase of Pseudomonas sp. measured in the presence of the alanine derivative of 2-naphthoic acid (NA-Ala) is inhibited by 1,10-phenanthroline, EDTA, bestatin and amastatin; this finding supports the conclusion that this enzyme is a metallo-aminopeptidase. A decrease of its activity in the presence of iodoacetamide and its activation by thiols points to the significant role of -SH groups in the regulation of its activity. Co2+, Ca2+ and Mg2+ ions increased the enzyme activity while Zn2+, Cd2+ and Pb2+ markedly inhibited the enzyme even at low concentrations. A high thermal stability of alanylaminopeptidase depended on the presence of 1 mmol/L Co2+ and of 1 mmol/L L-cysteine in the incubation mixture.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Jankiewicz U,Bielawski Wdoi
10.1007/BF02817643keywords:
subject
Has Abstractpub_date
2002-01-01 00:00:00pages
230-4issue
3eissn
0015-5632issn
1874-9356journal_volume
47pub_type
杂志文章abstract：:A rifampicin-resistant variant of two strains of Lactobacillus plantarum, one strain of Pediococcus acidilactici, and one strain of Enterococcus faecium were used for the experimental production of lucerne silage. Laboratory silage without inoculants served as a control. Counts of total anaerobes, total lactic acid ba...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-012-0142-5
更新日期：2012-07-01 00:00:00
abstract：:Streptomyces aureofaciens glucosidizes 1,2,4-trihydroxy-9,10-anthraquinone (purpurin) added to the cultivation medium to yield the corresponding 2-beta-D-glucoside. The identity of the glucoside was demonstrated by comparing its physico-chemical properties with data of an authentic sample prepared synthetically. A fur...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02873156
更新日期：1984-01-01 00:00:00
abstract：:Composition of sterol fractions of nystatin-resistant Candida maltosa strains was determined. Using UV-spectrometry, TLC and GLC-MS it was demonstrated that resistance to nystatin is connected with the composition alterations of yeast cell sterols. Block of different stages of ergosterol biosynthesis was revealed in s...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814494
更新日期：1991-01-01 00:00:00
abstract：:Purification of streptococcal extracellular NAD+ nucleosidase is associated with changes of kinetic properties. A high-molecular weight component is required for a full activity of the enzyme. The component is not produced by bacteria and is present in the Todd-Hewitt cultivation medium, the beef-heart extract serving...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02876396
更新日期：1980-01-01 00:00:00
abstract：:A new simple method used to eliminate polysaccharides that cause problems during DNA isolation was established for 6 different white-rot fungi using 1% hexadecyltrimethylammonium bromide (CTAB) as wash buffer and followed by centrifugation. Variation in the DNA yield and quality was ascertained using precipitating age...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931383
更新日期：2004-01-01 00:00:00
abstract：:The mean lengths of G1, S, G2 and M phases of the cell cycle were determined on the basis of the population distribution of Candida utilis grown in a continuous culture under steady-state conditions by using an original mathematical method. The length of the G2 phase was proportional to that of G1; the length of M was...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02821281
更新日期：1990-01-01 00:00:00
abstract：:Patients suffering from periodontitis or periodontosis were selected for the study. Further subdivision of these groups was based on the presence or absence of herpes and/or adenoviruses in their oral lymphocytes and epithelial cells. The phagocytic and bactericidal activities of oral leukocytes isolated from the same...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02887577
更新日期：1987-01-01 00:00:00
abstract：:Biochemical properties of a termostable alkaline phosphatase obtained from the mycelium extract of A. caespitosus were described. The enzyme was purified 42-fold with 32% recovery by DEAE-cellulose and concanavalin A-Sepharose chromatography. The molar mass estimated by Sephacryl S-200 or by 7% SDS-PAGE was 138 kDa an...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931303
更新日期：2007-01-01 00:00:00
abstract：:The uvrE (E. coli KS 114) cells carry a mutation in the gene that codes for helicase II. This is the protein responsible for replicative unwinding of double-helical DNA. The repair mode of such cells may be altered as compared with the wild type. The survival of uvrE cells during postirradiation incubation under inhib...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02922491
更新日期：1985-01-01 00:00:00
abstract：:Propionate at concentrations up to 0.05% decreased at 25 degrees C the growth and sporulation of Penicillium aurantiogriseum. The standard size of conidiogenous structures (metulae, phialids) and conidia was diminished. The effect was more pronounced at a higher temperature (30 degrees C). Inhibition of ochratoxin pro...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814202
更新日期：1995-01-01 00:00:00
abstract：:Using the method of two separate UV exposures the increase of UV resistance after various induction fluences in growing and resting Escherichia coli B/r Hcr+ was followed. In resting cells, the optimum induction energy fluence was found to be 30 J/m2. In exponentially growing cells testing of induction has proved to b...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02926452
更新日期：1979-01-01 00:00:00
abstract：:Dithiocarbamates were found to enhance the production of citric acid under solid-state fermentation conditions by Aspergillus niger. Maximum increase was observed with tetramethylthiuram disulfide (TMTD). Percent increases observed were 74.2% with 2.5 micrograms/mL of TMTD, 19.6% with 2.5 micrograms/mL of sodium dimet...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02926953
更新日期：1986-01-01 00:00:00
abstract：:Using phenol--water extraction, a lipopeptidopolysaccharide complex (LPPS) was isolated from Listeria monocytogenes. Some biological and immunological properties of LPPS were compared with lipopolysaccharide isolated by the same procedure from Salmonella typhimurium. LPPS possesses low pyrogenicity, but the immunologi...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02923515
更新日期：1985-01-01 00:00:00
abstract：:Cord and newborn sera were found to possess a significantly decreased in vitro bactericidal activity to Escherichia coli as compared to sera of adult individuals. Hyperbilirubinaemia in neonates suffering from both Rh and ABO incompatibility results in a further significant impairment of the bactericidal activity of t...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02927298
更新日期：1979-01-01 00:00:00
abstract：:Numerous antibodies with a known mechanism of action are utilized as possible means for studying morphogenesis and differentiation. Inhibitors of biosynthesis of nucleic acids and proteins, compounds intervening with the synthesis and/or function of cell walls and membranes or compounds influencing the energy metaboli...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/BF02897219
更新日期：1980-01-01 00:00:00
abstract：:Bifidobacteria (246 strains in total) were isolated from rectal samples of infants and adult humans and animals, and from intestinal samples of calves. Twenty-five strains grew well on mucin: 20 from infants, two from adults, and three from goatlings. Poor or no growth on mucin was observed in 156 bifidobacterial stra...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-011-0022-4
更新日期：2011-03-01 00:00:00
abstract：:A selection of tert-butylhydroperoxide (tBOOH)-tolerant Candida albicans mutants showed increased tolerances to 19 different stress conditions. These mutants are characterized by a constitutively upregulated antioxidative defense system and, therefore, adaptation to oxidative stress may play an important role in gaini...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-014-0305-7
更新日期：2014-07-01 00:00:00
abstract：:Different mechanistic models for potassium ion uptake are analyzed by an equilibrium-thermodynamic formalism in terms of their comparative efficiency in setting chemical potential differences of the potassium ion of different magnitudes across the plasma membrane of lower eukaryotic cells. The possible adaptive advant...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/BF02925852
更新日期：1988-01-01 00:00:00
abstract：:Virulence factors were compared in 15 Aeromonas spp. isolated from faeces of patients with Aeromonas-associated gastroenteritis and in 81 strains isolated from food. Strains from food did not show differences in the distribution of virulence factors when compared with strains isolated from faeces. However, 88.8% of Ae...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814323
更新日期：1994-01-01 00:00:00
abstract：:Sixty four biopsy specimens of oral Candida leukoplakias were examined. Histological sections were stained with periodic acid-Schiff (PAS) reagent and by Grocott's silver method. C. albicans isolates were cultivated from all the patients but fungal hyphae were proved histologically only in 23 of them. PAS-positive and...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814437
更新日期：2001-01-01 00:00:00
abstract：:The prevalence of extended-spectrum beta-lactamases (ESBL) was determined among isolates of Escherichia coli (n = 63) isolated from hospitalized (43) and healthy (20) children. Ten isolates (21%) were ESBL-positive for two screening tests, the double disk-synergy test and the Oxoid Combination Disk method. One ESBL-po...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02930963
更新日期：2003-01-01 00:00:00
abstract：:A series of experiments were performed with P. aeruginosa to demonstrate which of the biochemical mechanisms are responsible for the resistance to the beta-lactam antibiotics. The constitutive beta-lactamase was isolated and characterized for the strain used as type OXA whose pI was 7.1, with a molar mass of 49 kg/mol...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02877216
更新日期：1987-01-01 00:00:00
abstract：:A new metabolite was isolated from the culture of Penicillium rugulosum and its structure was determined from physico-chemical data. Accompanying metabolites skyrin and rugulosin were characterized by UV, IR, CD, mass and NMR spectra. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02876598
更新日期：1978-01-01 00:00:00
abstract：:Mice (strains Balb/c and A/J) received an intravenous infusion of bilirubin for a 1 d period. The infusion was delivered at various phases of the primary reaction; the degree of the immune response was expressed as the number of antibody-forming cells against sheep erythrocytes. Bilirubin infusion during both the indu...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02897214
更新日期：1980-01-01 00:00:00
abstract：:The newly described type strains Arcanobacterium pinnipediorum DSM 28752T and Arcanobacterium wilhelmae DSM 102162T, initially isolated from an anal swab of a harbor seal (Sammra et al. Int J Syst Evol Microbiol 65:4539-4543, 2015) and the genital tract of a rhinoceros (Sammra et al. Int J Syst Evol Microbiol 67:2093-...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-018-0610-7
更新日期：2018-11-01 00:00:00
abstract：:The validity of the principle of homeoviscous adaptation for Bacillus subtilis was tested by comparing fluorescence anisotropy (1,6-diphenyl-1,3,5-hexatriene) and electron-spin resonance (16-doxylstearate) measurements carried out in isolated plasma membranes and in phospholipid fractions. The physical measurements we...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02925901
更新日期：1988-01-01 00:00:00
abstract：:Changes in leukocyte counts and in the gut microflora of laboratory rats irradiated with single whole-body dose of gamma rays (5.0 Gy) were determined. The number of leukocytes was lower especially 1 and 2 weeks after irradiation. A significant decrease in lymphocytes was observed 1 week and in monocytes 1 and 2 weeks...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02818710
更新日期：2002-01-01 00:00:00
abstract：:The gene encoding the coat protein (CP) of a potato virus Y (PVY) was cloned into expression vector pMPM-A4Omega. PVY CP was expressed in Escherichia coli and the purified recombinant protein was used for raising rabbit polyclonal antibodies. The sera and antibodies were tested for the detection of PVY in the laborato...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-008-0067-1
更新日期：2008-01-01 00:00:00
abstract：:In the present study, the cDNA sequences of Hsp70 and Hsp90 genes of Isaria farinosa (designated IFHSP70 and IFHSP90) were cloned and characterized using multiple techniques of molecular biology and bioinformatics. The genetic differentiation of the two genes was investigated among 10 geographically separated populati...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-018-00676-x
更新日期：2019-07-01 00:00:00
abstract：:Differences in the effect of volatile and gaseous metabolites of germinating pea seeds on the germination of spores of Mucor racemosus and macroconidia of Fusarium oxysporum are described. Germination of spores of M. racemosus was inhibited by seed metabolites whereas germination of macroconidia of F. oxysporum was st...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02933019
更新日期：1980-01-01 00:00:00
